- Tytuł:
- Detection and molecular identification of Cryptosporidium species in laboratory rats (Rattus norvegicus) in Ibadan, Nigeria
- Autorzy:
-
Ayinmode, Adekunle Bamidele
Ogbonna, Nkeiruka Fortunate
Widmer, Giovanni - Powiązania:
- https://bibliotekanauki.pl/articles/972090.pdf
- Data publikacji:
- 2017
- Wydawca:
- Polskie Towarzystwo Parazytologiczne
- Tematy:
-
laboratory rat
microscopy
pcr
sequencing
Cryptosporidium species - Opis:
- To study the occurrence of Cryptosporidium infection in laboratory rats (Rattus norvegicus) raised for experimental usage, 134 faecal samples were obtained from two rearing houses in Ibadan and examined for the presence of Cryptosporidium oocyst using the modified acid fast staining technique. Cryptosporidium species in 2 samples positive for microscopy were further characterized by a nested polymerase chain reaction (PCR) amplifying the 18S rRNA gene. Two of 134 samples were positive for the Cryptosporidium oocysts. Sequencing of the small-subunit rRNA amplicons identified the species in the two PCR positive samples as Cryptosporidium andersoni and Cryptosporidium rat genotype. These findings showed that laboratory rat is a potential reservoir for diverse Cryptosporidium species and suggests that laboratory rats should be screened for Cryptosporidium infection prior to experiments, especially where pathogen free animals are not available. This the first report to identify Cryptosporidium species infecting laboratory rats in Nigeria.
- Źródło:
-
Annals of Parasitology; 2017, 63, 2; 105-109
0043-5163 - Pojawia się w:
- Annals of Parasitology
- Dostawca treści:
- Biblioteka Nauki
Artykuł