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    Wyświetlanie 1-2 z 2
    Tytuł:
    The influence of oxalate decarboxylase on the urinary oxalate excretion in swine model of nephrocalcinosis induced by hydroxyproline
    Autorzy:
    Pierzynowska, Katerina
    Pierzynowski, Stefan G.
    Lozinska, Liudamyla
    Jarmakiewicz, Sara
    Świeboda, Paulina
    Fedkiv, Olexandr
    Szwiec, Katarzyna
    Piedra, Jose Louis Valverde
    Filip, Rafal
    Powiązania:
    https://bibliotekanauki.pl/articles/454959.pdf
    Data publikacji:
    2017
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    Nephrocalcinosis
    Oxalate decarboxylase
    Hydroxyproline
    Pig model
    Opis:
    Introduction. Kidney stone formation may be a result of increased urinary oxalate supersaturation. Material and Methods. Eighteen pigs were randomly divided into: Control group, where standard cereal-based feed was supplemented with 4% HP only, Prevention group, where treatment with OxDc slurry started at the end of the adaptation period when pigs were switched to 4% HP diet, Reduction group, where the treatment with OxDc lyo powder started after pigs were already on a 4% HP diet for 6 days. Results. OxDc slurry prevented oxalate excretion in urine. The reduction effect of OxDc lyo feed addition was generally visible during the first two days of the therapy (p<0.05). Both dietary intake of 4% HP and OxDc preparations did not influence weight gain, water or feed intake, urine excretion and creatinine clearance. Conclusions. The capacity of OxDc in preventing induced hyperoxaluria was moderate. Most probably, this is due to the incoherent response of animals to the HP enriched diet dependent on their gut pH, since optimum pH for OxDc is around 5-6. A higher pH essentially reduces the activity of OxDc. The capacity of OxDc in reversing the hyperoxaluria induced by a HP enriched diet was significant during the first 2 days after introducing OxDc to the diet.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2017, 3; 206-2016
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Model development of hydroxyproline induced hyperoxaluria in young growing pigs
    Autorzy:
    Goncharova, Kateryna
    Filip, Rafał
    Świeboda, Paulina
    Prykhodko, Olena
    Fedkiv, Olexandr
    Szwiec, Katarzyna
    Piedra, Jose Luis Valverde
    Majda, Blanka
    Pierzynowski, Stefan Grzegorz
    Powiązania:
    https://bibliotekanauki.pl/articles/454832.pdf
    Data publikacji:
    2017
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    pig model
    hyperoxaluria
    kidney stones
    hydroxyproline
    Opis:
    Aim of the study. In this study, we sought to create a model of reversible hyperoxaluria in pigs by feeding with hydroxyproline (HP). Materials and methods. The experiment included 12 pigs divided into 2 groups (n = 6). The pigs were fed twice a day. At the beginning of the experiment, in the adaptation period, all pigs were given standard feed. In the next 7 days, an increasing amount of hydroxyproline (1–3% HP), was added to the feed. In next 14 days, 4% HP was administered in each pig meal. After 14 days of 4% HP diet, the pigs were randomly divided into 2 groups. For 6 pigs, 4% HP treatment had been continued for the next 14 days while the second group of pigs for the next 14 days received a standard HP free diet. 24h urine samples, blood and fecal samples were collected on particular days. Results. The addition of HP to the diet increased urinary oxalate excretion. A characteristic increase was noted after 12 days of treatment with 4% HP. During the removal period, oxalate excretion decreased in the group without HP in diet, while in the group which continued with a 4% HP diet, oxalate excretion significantly increased. Gross examination of kidneys showed that in the group which had 4% HP diet for 4 weeks, kidneys were fibrotic with enlarged cavities, and had small visible urinary stones. In second group, kidneys were relatively normal looking with no visible stones. Conclusion. Hyperoxaluria is reversible, if HP is removed 14 days after the start of 4% HP diet. Prolonged exposure up to 4 weeks causes pathologic changes in kidneys including crystals, sand and small stone formation.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2017, 1; 6-11
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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