Autor: Łojkowska, Ewa - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Antibacterial activity of caffeine against plant pathogenic bacteria
Autorzy:: Sledz, Wojciech
Los, Emilia
Paczek, Agnieszka
Rischka, Jacek
Motyka, Agata
Zoledowska, Sabina
Piosik, Jacek
Lojkowska, Ewa
Powiązania:: https://bibliotekanauki.pl/articles/1039014.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: antibacterial activity
plant secondary metabolites
Ralstonia solanacearum
Clavibacter michiganesis subsp. sepedonicus
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas syringae pv. tomato and Xanthomonas campestris subsp. campestris
Opis:: The objective of the present study was to evaluate the antibacterial properties of a plant secondary metabolite - caffeine. Caffeine is present in over 100 plant species. Antibacterial activity of caffeine was examined against the following plant-pathogenic bacteria: Ralstonia solanacearum (Rsol), Clavibacter michiganesis subsp. sepedonicus (Cms), Dickeya solani (Dsol), Pectobacterium atrosepticum (Pba), Pectobacterium carotovorum subsp. carotovorum (Pcc), Pseudomonas syringae pv. tomato (Pst), and Xanthomonas campestris subsp. campestris (Xcc). MIC and MBC values ranged from 5 to 20 mM and from 43 to 100 mM, respectively. Caffeine increased the bacterial generation time of all tested species and caused changes in cell morphology. The influence of caffeine on the synthesis of DNA, RNA and proteins was investigated in cultures of plant pathogenic bacteria with labelled precursors: [3H]thymidine, [3H]uridine or 14C leucine, respectively. RNA biosynthesis was more affected than DNA or protein biosynthesis in bacterial cells treated with caffeine. Treatment of Pba with caffeine for 336 h did not induce resistance to this compound. Caffeine application reduced disease symptoms caused by Dsol on chicory leaves, potato slices, and whole potato tubers. The data presented indicate caffeine as a potential tool for the control of diseases caused by plant-pathogenic bacteria, especially under storage conditions.
Źródło:: Acta Biochimica Polonica; 2015, 62, 3; 605-612
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Triterpenoid α-amyrin stimulates proliferation of human keratinocytes but does not protect them against UVB damage
Autorzy:: Biskup, Edyta
Gołębiowski, Marek
Gniadecki, Robert
Stepnowski, Piotr
Łojkowska, Ewa
Powiązania:: https://bibliotekanauki.pl/articles/1039744.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: UVB
BrdU incorporation
Rhaponticum carthamoides
α-amyrin
antioxidant activity
Opis:: Rhaponticum carthamoides plants ("maral root") are widely used in Siberian folk medicine. The present study reports for the first time the presence of pentacyclic terpenoid, α-amyrin, in methanol extract from leaves of this plant. α-Amyrin induced proliferation of human keratinocytes (HaCaT) by about 18% while other extract components were ineffective. A panel of biochemical and cell-based assays testing the antioxidative and cytoprotective activites of α-amyrin indicated no antioxidative activity of this compound. α-Amyrin did not protect HaCaT cells against the damage caused by UVB radiation.
Źródło:: Acta Biochimica Polonica; 2012, 59, 2; 255-260
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Detection and identification of potentially toxic cyanobacteria in Polish water bodies
Autorzy:: Głowacka, Joanna
Szefel-Markowska, Magdalena
Waleron, Małgorzata
Łojkowska, Ewa
Waleron, Krzysztof
Powiązania:: https://bibliotekanauki.pl/articles/1039881.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: mcyB
microcystins
toxins
16S rRNA
Cyanobacteria
mcyE
rpoC1
Opis:: The main goal of this study was to determine the distribution of potentially toxic cyanobacteria in 39 selected Polish water bodies. From the water bodies with blooms and also from those in which blooms were not visible 87 samples were investigated. For the first time samples from ponds localized in villages with high agricultural activities were included. Lakes for which microcystin concentrations had been determined before were included as a reference for the research. The detection of cyanobacteria was conducted by microscopic observation as well as by PCR amplification of the rpoC1 gene fragment. Cyanobacteria were present in 75 out of 87 samples. The presence of potentially toxic cyanobacteria was detected by amplification of the mcyB and mcyE genes, which are involved in the biosynthesis of microcystins. Both genes were detected in 7 out of 9 blooms investigated. In the case of samples collected from water bodies in which blooms were not observed, the mcyB and mcyE genes were detected in 20 out of 36. In order to identify the cyanobacteria occurring in selected reservoirs, 16S plus ITS clone libraries were constructed. The method allowed distinguishing 18 different genotypes. After sequence analysis, cyanobacteria belonging to genera Microcystis, Planktothrix, Anabaena, Pseudanabaena, Synechocystis, Synechococcus and Woronichinia were identified. Results confirmed the usefulness of the rpoC1 and mcy genes for monitoring water bodies and detection of potentially toxic cyanobacteria. Application of molecular markers allowed detecting potentially toxic cyanobacteria before the bloom was visible. This is the first comprehensive study concerning cyanobacteria present in different types of Polish water bodies performed using molecular markers.
Źródło:: Acta Biochimica Polonica; 2011, 58, 3; 321-333
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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