- Tytuł:
- Production of yeast biomass with elevated content of glutathione
- Autorzy:
-
Udeh, K. O.
Achremowicz, B. - Powiązania:
- https://bibliotekanauki.pl/articles/1372456.pdf
- Data publikacji:
- 1994
- Wydawca:
- Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
- Tematy:
-
biochemia
cysteina
siarczan amonowy zob.siarczan amonu
siarczan amonu
biosynteza
glutation
biomasa
sacharoza
pozywki
Saccharomyces cerevisiae
aminokwasy - Opis:
- Research on biosynthesis of glutathione (GSH) by yeast Saccharomyces cerevisiae was undertaken with the aim of achieving high content of this compound intracellularly. The effect of GSH-amino-acids free and GSH-amino-acids enriched media added alone or in combination at different concentrations were examined. Furthermore, the combined effect of carbon and sulphur were also investigated. Cultivations were performed in shake-flasks at 28°C for 24-48 h in a synthetic medium with saccharose and ammonium sulphate as the sources of carbon and sulphur. The strain Saccharomyces cerevisiae cultured in GSH-amino-acids enriched media accumulated about 11-20 mg GSH/g d:ry biomass and the content of biomass was 6.5-12.5 g dry biomass/dm3 after 24 h of cultivation. Cysteine added alone to the medium was confirmed as the key amino-acid responsible for the increased biosynthesis of GSH in the yeast cell. In addition, increase in the sulphur content exerted a marked positive effect on its biosynthesis.
- Źródło:
-
Polish Journal of Food and Nutrition Sciences; 1994, 03, 1; 93-100
1230-0322
2083-6007 - Pojawia się w:
- Polish Journal of Food and Nutrition Sciences
- Dostawca treści:
- Biblioteka Nauki
Artykuł