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Tytuł:
A fast and effective protocol for obtaining genetically diverse stevia (Stevia rebaudiana Bertoni) regenerants through indirect organogenesis
Szybki i efektywny protokół otrzymywania zróżnicowanych genetycznie regenerantów stewii (Stevia rebaudiana Bertoni) drogą pośredniej organogenezy
Autorzy:
Dyduch-Siemińska, M.
Powiązania:
https://bibliotekanauki.pl/articles/13925501.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
stevia
Stevia rebaudiana
micropropagation
molecular marker
random amplified polymorphic DNA
shoot regeneration
somaclonal variation
Źródło:
Agronomy Science; 2021, 76, 4; 47-62
2544-4476
2544-798X
Pojawia się w:
Agronomy Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adventitious shoot regeneration from leaf explants of Malus domestica Borkh. in vitro
Autorzy:
Podwyszynska, M.
Sowik, I.
Powiązania:
https://bibliotekanauki.pl/articles/951259.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
shoot regeneration
leaf explant
Malus domestica
polyploidization
apple
Free Redstar cultivar
Gala Must cultivar
Sander cultivar
Red Chief cultivar
Pristina cultivar
growth regulator
cytological analysis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Artemisia species in vitro cultures for production of biologically active secondary metabolites
Autorzy:
Grech-Baran, M.
Pietrosiuk, A.
Powiązania:
https://bibliotekanauki.pl/articles/80902.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Artemisia
in vitro culture
hairy root
bioreactor
natural drug
arglabin
artemisinin
plant regeneration
callus culture
shoot culture
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological factors affecting regeneration of adventitious shoots from in vitro isolated ligulate florets of chrysanthemum
Biologiczne czynniki wpływające na regenerację pędów przybyszowych z izolowanych in vitro kwiatów języczkowatych chryzantemy
Autorzy:
Tymoszuk, A.
Zalewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/11542912.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
Dendranthema grandiflora
biological factor
plant regeneration
shoot
in vitro
ligulate floret
chrysanthemum
organogenesis
inflorescence
explant
inoculation
Opis:
In mutation breeding of chrysanthemum the regeneration in vitro of adventitious shoots from ligulate florets can lead to the separation of chimera components and, as a result, to producing a new original cultivar. The success of that method considerably depends on the result being the number of the shoots formed. The more is produced, the greater the chances for an effective separation of chimera components and creating a new stable cultivar. The present research defines the effect of such factors as the inflorescence development stage, the type of the explant as well as the position of its inoculation on the increase in the efficiency of adventitious shoots regeneration. The ligulate florets of Chrysanthemum × grandiflorum (Ramat.) Kitam. ‘Cool Time’ were inoculated on the Murashige and Skoog [1962] medium supplemented with 2 mg·dm-3 BAP and 0.5 mg·dm-3 NAA. There was shown no significant effect of the inflorescence development stage (incompletely open with a partially visible disk or with the entire visible disk in which tubular florets do not produce pollen or completely open in which two or half of the whorls of tubular florets produce pollen) on the shoot regeneration efficiency. Most shoots regenerate on transversely- or lengthwise-cut into half or on the entire pierced ligulate florets – horizontally inoculated, with the abaxial side on the medium.
W hodowli mutacyjnej chryzantem regeneracja in vitro pędów przybyszowych z kwiatów języczkowatych doprowadzić może do rozdzielenia komponentów chimer i w rezultacie do uzyskania nowych, oryginalnych odmian. W tej metodzie o sukcesie w dużej mierze decyduje wynik w postaci liczby uzyskanych pędów. Im większa jest ich liczba, tym większe są szanse na skuteczną separację komponentów chimer i uzyskanie nowej stabilnej odmiany. W badaniach określono wpływ takich czynników jak stadium rozwoju kwiatostanu, rodzaj eksplantatu oraz pozycja jego inokulacji na zwiększenie wydajności kaulogenezy przybyszowej. Kwiaty języczkowate Chrysanthemum × grandiflorum (Ramat.) Kitam. ‘Cool Time’ wykładano na pożywka Murashige i Skooga [1962] uzupełnioną 2 mg·dm-3 BAP i 0.5 mg·dm-3 NAA. Nie udowodniono wpływu stadium rozwoju kwiatostanu (niecałkowicie otwarty z widocznym częściowo oczkiem lub z widocznym całym oczkiem, w którym nie pylą kwiaty rurkowate albo całkowicie otwarty, w którym dwa lub połowa okółków kwiatów rurkowatych pyli) na wydajność regeneracji pędów. Najwięcej pędów powstaje na przeciętych poprzecznie lub podłużnie na pół albo na całych nakłuwanych kwiatach języczkowatych inokulowanych horyzontalnie stroną zewnętrzną.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 3; 155-165
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Conservation of the endangered medicinal plant Picrorhiza kurroa through in vitro multiple shoot regeneration
Autorzy:
Chaudhary, V.
Singh, S.
Sharma, R.
Singh, A.
Sharma, N.
Powiązania:
https://bibliotekanauki.pl/articles/80933.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
medicinal plant
Picrorhiza kurroa
endangered plant
shoot regeneration
micropropagation
conservation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of thidiazuron on in vitro shoot regeneration of Morus alba
Autorzy:
Rezaei-Zafarghandi, M.S.
Rahmati-Joneidabad, M.
Powiązania:
https://bibliotekanauki.pl/articles/2096960.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mulberry
cotyledon
plant growth regulator
organogenesis
in vitro-grown seedlings
Opis:
Mulberry (Morus alba L.) is an ornamental, medicinal, and fruit plant that belongs to the Moraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which enables mass production of pathogen-free plants. Cotyledon has a high potential for shoot regeneration; however, to the best of our knowledge, there are no reports on plant regeneration from cotyledon explants of mulberry. Therefore, this study aimed to evaluate the potential of seedling-derived cotyledon segments to obtain shoot multiplication of mulberry. Various concentrations (0, 0.25, 0.5, 1, 2, 3, and 4 mg/l) of thidiazuron (TDZ) in combination with indole butyric acid (IBA) were used in a completely randomized design in three replications. The results showed that the highest percentage of regeneration frequency (96.67%) and the maximum number of shoots (4.43) were obtained on Murashige and Skoog (MS) medium supplemented with 0.25 mg/l TDZ and 0.025 mg/l IBA. In the rooting experiment, the maximum rooting percentage (83.33%) and the maximum number of roots per shoot (4.36) were obtained on MS medium containing 2 mg/l IBA. In vitro-raised plantlets were placed in pots and kept in room temperature for 30 days, and the plantlets showed more than 90% survival rate. On the basis of our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 55-61
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects thidiazuron and paclobutrazol on regeneration potential of tulip flower stalk explants in vitro and subsequent shoot multiplication
Autorzy:
Podwyszynska, M
Marasek, A.
Powiązania:
https://bibliotekanauki.pl/articles/58430.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
flower
micropropagation
somatic embryogenesis
tulip
paclobutrazol
thidiazuron
regeneration
new cultivar
modern breeding
in vitro
tulip cultivar
shoot multiplication
Opis:
The effects of TDZ and paclobutrazol on the primary regeneration on tulip flower stalk explants of six cultivars and subsequent shoot multiplication were examined. Explants, flower stalk slices, were excised from cooled and subsequently forced bulbs. The explants were incubated for two months in darkness on medium containing NAA and cytokinins, 2iP and BAP, as control, or TDZ (0.5-4 mg l-1) and paclobutrazol (0.05-0.4 mg l-1). Then, the regenerating explants were subcultured on medium with TDZ and NAA applied at low concentrations. Different regeneration capabilities were found depending on cultivar and growth regulators. The percentage of explants forming leaf-like structures ranged, on the control medium, from 80% in 'Blue Parrot' and 'Prominence' to below 30% in 'Apeldoorn' and 'Mirjoran'. TDZ, applied at optimum for each cultivar concentration, greatly increased the regeneration potential up to 70-100%. Paclobutrazol, added to the TDZ-containing medium, significantly enhanced the response of explants, resulting in high numbers of leaf-like structures formed per explant (13.7-22.8). The structures developed gradually into characteristic forms: the growing up cotyledonary leaf, the probable root primordium formed at its base, the growing downwards stolon and the shoot meristem developed finely on its tip. It is suggested that such primary regeneration may have a nature of somatic embryogenesis. Then, the adventitious shoots developed and formed clusters, which were divided into 2-3 smaller ones every two months. The growth regulators, used at initial stage, markedly influenced subsequent shoot multiplication. Thus, the most intensive shoot formation was noted with TDZ at concentrations of 0.5-2 mg l-1 and paclobutrazol of 0.05-0.1 mg l-1.
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficient in vitro plant regeneration from cultured leaf and petiole explants of Isatis constricta Davis
Autorzy:
Özel, Ç.A.
Powiązania:
https://bibliotekanauki.pl/articles/11867960.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
Turkey
Brassicaceae
Isatis constricta
plant regeneration
shoot regeneration
leaf
petiole
in vitro method
plant propagation
micropropagation
Opis:
Isatis concstricta Davis (an endemic plant of Turkey) suffers from low propagation rates under natural conditions and is threatened due to fast unplanned urbanisation. The study compared the effects of variants of BA + NAA on shoot regeneration on leaf and petiole explants excised from one week old in vitro regenerated seedlings. MS medium containing 1 mg L–1 BA + 1 mg L–1 NAA induced maximum proliferation on petiole and leaf explants with 13.33 and 12.75 shoots per explant repectively. However, leaf explant induced shoots were sturdy and healthier compared to petiole explant induced shoots. These shoots were rooted on MS medium containing 0.5 mg L–1 IBA and the plants were acclimatized in peat moss and sand (v/v). They grew to flowering under ex vitro conditions. This system of regeneration is advantageous for conventional propagation and the results will help in establishment of a powerful and meaningful micropropagation system for I. constricta.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 3; 49-55
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enhanced mass regeneration of pro-vitamin A cassava (Manihot esculenta Crantz) varieties through multiple shoot induction from enlarged axillary buds
Autorzy:
Opabode, J.T.
Powiązania:
https://bibliotekanauki.pl/articles/80396.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mass propagation
provitamin A
cassava
Manihot esculenta
genetic fidelity
6-benzylaminopurine
alpha-naphthalene acetic acid
indole butyric acid
shoot induction
axillary bud
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro morphogenic events in culture of Lotus corniculatus L. seedling root explants
Autorzy:
Rybczynski, J J
Karolkowska, M.
Kaczmarek, Z.
Mikula, A.
Fiuk, A.
Powiązania:
https://bibliotekanauki.pl/articles/57212.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
in vitro culture
morphogenic event
Lotus corniculatus
seedling root explant
plant regeneration
cytokinin
callus proliferation
shoot differentiation
root explant
Opis:
The experiments were carried out on Lotus corniculatus (L.) seedling root explants of the cultivar varieties Skrzeszowicka, Caroll A10 and strain 175. Callus formation and shoot regeneration were the major explant response depended mainly on of the studied genotype and used plant growth regulators (PGRs). Primary cortex of proximal and distal end of explant was the most active tissue for callus proliferation. For shoot primordia differentiation deeper zones of cortex took a part. The process of meristematic centre initiation was not uniform and various level of shoot differentiation events were observed not earlier than 3 weeks of culture. Usually, the shoot primordia regeneration began on proximal rather than distal end of the explant. BAP rather than urea derivatives stimulated shoot proliferation in extended cultures. Increasing of BAP and TDZ concentrations brought about the explant polarity and expansion of the meristematic zones. The explant position in root did not have significant influence on the number of regenerated shoots. The cultures only had better bud formation by TDZ when compared to BAP. BAP stimulated bud formation and development of the shoots from them. Short term of TDZ treatment of explants stimulated meristem formation which developed into buds and shoots. CPPU stimulated callus proliferation and bud formation when explants pretreatment was prolonged from 12 to 36 hrs.
Źródło:
Acta Societatis Botanicorum Poloniae; 2006, 75, 3; 191-200
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis
Autorzy:
Chornobrov, Oksana
Bilous, Svitlana
Powiązania:
https://bibliotekanauki.pl/articles/2041281.pdf
Data publikacji:
2021
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
Christmas cactus
nutrient medium
explant
callus
shoot proliferation
microclonal propagation
Opis:
Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl2 for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2021, 63, 1; 68-73
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro regeneration of Centaurium erythraea Rafn from shoot tips and other seedling explants
Autorzy:
Piatczak, E
Wysokinska, H.
Powiązania:
https://bibliotekanauki.pl/articles/56437.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Centaurium erythraea
micropropagation
seedling explant
shoot tip
regeneration
organogenesis
in vitro
Gentianaceae
Opis:
Various explants from 30-day-old seedlings of Centaurium erythraea Rafn were evaluated for their morphogenetic capacity under in vitro culture conditions. Shoot formation from shoot tip explants was achieved mainly through adventitious bud differentiation. The highest number of shoots (up to 43.3 ± 2.2 from a single shoot tip) was obtained on Murashige and Skoog medium (MS) supplemented with indole-3-acetic acid (IAA) (0.57 μM) and 6-benzylaminopurine (BAP) (4.4 μM). Adventitious shoot regeneration was also achieved through organogenesis from calluses obtained from hypocotyls, cotyledons, roots and leaves on MS medium containing IAA (2.85 μM) and BAP (0.88 μM). Significant differences were noted between explant types in their effects on shoot regeneration. In the primary culture, the best response was obtained either from calluses derived from roots or leaves (44.4 ± 4.5 and 40.2 ± 6.0 shoots per callus, respectively). The number of subcultures of inoculated calluses affected both the multiplication rate (the number of shoots/explant) and shoot morphology (the frequency of shoot hyperhydricity). Shoots rooted with the frequency of 94-100% after culture on MS medium without growth regulators. Plantlets were successfully acclimatized (97%) under high relative humidity and then moved to the greenhouse.
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro regeneration of Plantago media L. from shoot tips and other seedling explants
Autorzy:
Budzianowska, A.
Malkiewicz, M.
Budzianowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/951282.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Plantago media
in vitro
regeneration
shoot tip
seedling explant
propagation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro regeneration of Vitellaria paradoxa from shoot tip explants
Autorzy:
Afful, N.T.
Abdulai, I.
Azu, E.
Elegba, W.
Annor, W.
Akama, C.
Asare, K.
Dentey, J.
Amoatey, H.M.
Powiązania:
https://bibliotekanauki.pl/articles/2096257.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Vitellaria paradoxa
shea
shoot
root
survival
Opis:
Shea tree (Vitellaria paradoxa) is an essential tree crop with great potential economic value mainly because of its seed oil (shea butter) which is of high demand for manufacturing assorted products in food, cosmetic, and rubber industries. Propagation of this species is, however, hindered by relative unavailability of seed (nuts), erratic seed germination, a long vegetative phase, and latex exudation from cuttings. Thus, another method of propagation through in vitro culture is recommended for rapid multiplication of shea genotypes for large-scale cultivation. In the present study, the effects of two cytokinins, namely, 6-benzylaminopurine (BAP) and kinetin (KIN), and one auxin, namely 1-naphthaleneacetic acid (NAA), on shoot and/or root induction in vitro were assessed at various combinations/concentrations. The inclusion of these growth regulators in the culture medium significantly improved (P < 0.05) shoot/root regeneration over the controls. The highest shoot regeneration percentage (100%) was obtained on Murashige and Skoog (MS) basal medium supplemented with 2 mg * dm^-3 KIN + 0.5 mg * dm^-3 NAA or 1.5 mg * dm^-3 KIN within 7/8 days of inoculation. This medium (2 mg * dm^-3 KIN + 0.5 mg * dm^-3 NAA) showed the highest mean shoot length of 3.24 cm. Compared to KIN, BAP was more effective in inducing vigorous shoot growth. However, rooting was induced only on MS medium modified with 1 mg * dm^-3 BAP + 0.5 mg * dm^-3 NAA. These findings can serve as baseline information for in vitro, commercial scale propagation of shea tree.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 1; 71-79
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants
Autorzy:
Jafari, M.
Daneshvar, M.H.
Lotfi, A.
Powiązania:
https://bibliotekanauki.pl/articles/80666.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Passiflora caerulea
shoot proliferation
in vitro
acclimatization
plant growth regulator
regeneration
rooting
shoot explant
shoot multiplication
indole butyric acid
thidiazuron
cytokinin
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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