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Tytuł:
Znaczenie aktywności proteazy kapsydowej CP w rozwoju infekcji alfawirusowych
The role of capsid protease CP activity in the development of alphaviral infections
Autorzy:
Torzyk, Karolina
Skoreński, Marcin
Sieńczyk, Marcin
Powiązania:
https://bibliotekanauki.pl/articles/2200548.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
alfawirusy
arbowirusy
proteazy serynowe
proteaza kapsydowa CP
inhibitory
alphaviruses
arboviruses
serine proteases
capsid protease CP
inhibitors
Opis:
Alphaviruses belong to the worldwide distributed Togaviridae family and Alphavirus genus. They are spherical, enveloped, single-stranded RNA arthropodborne viruses. Alphaviruses are mostly transmitted by mosquitoes (Aedes spp. and Anopheles spp.) and are geographically distributed in restricted areas where appropriate vectors are present (Fig.1.). The most recognized members of this genus are Sindbis (SINV), Semliki Forest (SFV), Venezuelan equine encephalitis (VEEV), Ross River (RRV), and Chikungunya (CHIKV) viruses. Alphaviruses are infection agents for humans and many animals. Clinically, most human infections with arthritogenic alphaviruses are associated with symptoms such as fever, headache, joint pain, rash, chronic arthritis, and encephalitis. Major events during the alphaviral infection are virus entry, replication, assembly, and budding of new virions. Alphaviral RNA encodes four nonstructural and five structural proteins. Nonstructural proteins are mainly involved in the replication process and virus pathogenesis, while structural proteins form new virions. Both groups of viral proteins are produced as single polyproteins which undergo autoproteolytic maturation. This process is carried out by the two viral proteases, cysteine protease nsP4 and C protein serine protease (CP), and is considered to be critical for virus replication. The capsid protease CP is a chymotrypsin-like serine protease with the catalytic triad including His145, Asp167, and Ser219. What is important, after a suicidal autoproteolytic event the side chain of Trp267 remains bound in a hydrophobic S1 pocket thus inhibiting further trans-proteolytic activity. Alphaviral capsid protein undergoes a single proteolytic reaction before maturation and then, after selfinactivation, it assembles to form a viral capsid shell. Inhibitors of the capsid protease have significant antiviral activity. Compounds belonging to this group can be good candidates for new antiviral drugs.
Źródło:
Wiadomości Chemiczne; 2022, 76, 5-6; 309--321
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ zakażenia larwami III stadium Anisakis simplex na aktywność proteaz w przewodzie pokarmowym świnek morskich
THE INFLUENCE OF INFECTION WITH III LARVAL STAGE OF ANISAKIS SIMPLEX ON THE ACTIVITY OF PROTEASES IN ALIMENTARY TRACT OF GUINEA PIGS*
Autorzy:
Dziekońska-Rynko, J.
Rokicki, J.
Jabłonowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2148919.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przewod pokarmowy
enzymy proteolityczne
Anisakis simplex
aktywnosc enzymatyczna
parazytologia
inwazja pasozytnicza
proteaza
swinki morskie
larwy
Opis:
The studies were carried out on guinea pig males. The animals were infected with 30 larvae (L3) of Anisakis simplex. After 6 hrs of invasion the animals were dissected. In homogenized pancreas and duodenal contents activities of trypsin were determined. In stomach content activities of pepsin were determined. The activities of trypsin in duodenal contents and in pancreas homogenate from infected animals were lower in comparison with the control animals. The activities of pepsin were higher in infected animals.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 4; 425-430
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Was the serine protease cathepsin G discovered by S. G. Hedin in 1903 in bovine spleen?
Autorzy:
Palesch, David
Sieńczyk, Marcin
Oleksyszyn, Jozef
Reich, Michael
Wieczerzak, Ewa
Boehm, Bernhard
Burster, Timo
Powiązania:
https://bibliotekanauki.pl/articles/1039945.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
spleen cells
Hedin
cathepsin
proteases
Opis:
In the beginning of the 20th century, enzymes with proteolytic activity were classified as peptidases, Erepsin, and proteases. Among these, pepsin, trypsin, and autolytic enzymes were of the protease class. Spleen-derived proteases were poorly characterized until Sven Gustaf Hedin performed several digestion experiments with bovine spleen. He incubated minced bovine spleen under acidic or neutral conditions and characterized two active proteases; the results were published in 1903. The first protease was named α-protease and was active under neutral conditions. The second was named β-protease and was active under acidic conditions. We replicated Hedin's experiments according to his methods and found, by using activity-based probes to visualize proteases, that the historical α-protease is the present-day serine protease cathepsin G (CatG), which is known to be important in several immune processes, including antigen processing, chemotaxis, and activation of surface receptors. The β-protease, however, comprised different proteases including CatX, B, S, and D. We suggest that Hedin described CatG activity in bovine spleen over 100 years ago.
Źródło:
Acta Biochimica Polonica; 2011, 58, 1; 39-44
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Three Pseudomonas aeruginosa strains with different protease profiles
Autorzy:
Andrejko, Mariola
Zdybicka-Barabas, Agnieszka
Janczarek, Monika
Cytryńska, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1039614.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aprA
alkaline protease
extracellular proteases
elastase B
virulence
lasB
Pseudomonas aeruginosa
Opis:
The proteolytic activity of three Pseudomonas aeruginosa strains, ATCC 27853 - a reference strain, and two clinical isolates was tested. The activity was examined after culturing the bacteria in two different growth media: the minimal M9 medium and rich Luria-Bertani broth (LB). Based on zymograms and protease activity specific assays, it was concluded that the reference strain produced three proteolytic enzymes in the LB medium: protease IV, elastase B and elastase A, while alkaline protease was only produced in the M9 medium. The clinical isolates of P. aeruginosa produced elastase B and alkaline protease when grown in the LB medium and the minimal M9 medium, respectively. PCR analysis confirmed the presence of both the lasB gene encoding elastase B and aprA coding for alkaline protease in the genomes of the three P. aeruginosa strains analyzed. The expression of these genes coding for two important P. aeruginosa virulence factors was dependent on the growth conditions in all the strains studied. The contribution of the extracellular proteinases to the virulence of P. aeruginosa strains used in this study was investigated using an insect model, the greater wax moth Galleria mellonella.
Źródło:
Acta Biochimica Polonica; 2013, 60, 1; 83-90
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Theoretical studies of binding modes of two covalent inhibitors of cysteine proteases.
Autorzy:
Drabik, Piotr
Politowska, Ewa
Czaplewski, Cezary
Kasprzykowski, Franciszek
Łankiewicz, Leszek
Ciarkowski, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1044228.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cysteine proteases
covalent protease inhibitors
constrained simulated annealing
papain
molecular dynamics
Opis:
Physiological and pathological roles of cysteine proteases make them important targets for inhibitor development. Although highly potent inhibitors of this group of enzymes are known, their major drawback is a lack of sufficient specificity. Two cysteine protease covalent inhibitors, viz. (i) Z-RL-deoxo-V-peptide-epoxysuccinyl hybrid, and (ii) Z-RLVG-methyl-, have been developed and modeled in the catalytic pocket of papain, an archetypal thiol protease. A number of configurations have been generated and relaxed for each system using the AMBER force field. The catalytic pockets S3 and S4 appear rather elusive in view of the observed inhibitors' flexibility. This suggest rather limited chances for the development of selective structure-based inhibitors of thiol proteases, designed to exploit differences in the structure of catalytic pockets of various members of this family.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 1061-1066
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The total protein content, protein fractions and proteases activities of drone prepupae of Apis mellifera due to varrosis
Autorzy:
Żółtowska, K.
Lipiński, Z.
Dmitryjuk, M.
Powiązania:
https://bibliotekanauki.pl/articles/2146371.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
protease
protease activity
honey bee
drone prepupa
protein fraction
varrosis
total protein
Varroa destructor
Apis mellifera
protein content
Opis:
The proteins level and activities of acid and alkaline proteases in whole body extracts of drone prepupae of Apis mellifera naturaly infested with Varroa destructor were studied. The infested and a non-infested group did not differ significantly in their total protein content. However, some differences in protein profiles were found. A lack of three protein fractions of moderate and lower molecular weight in infested prepupae was noted. Moreover, some differences in the quantity of protein in most of the fractions were observed. The activity of acid proteases from infested prepupae was lower (p < 0.05) compared with the activity of these proteases from the non-infested one group. The infested drone had higher activity of alkaline proteases than non-infested but this difference was not statisticaly significant.
Źródło:
Wiadomości Parazytologiczne; 2005, 51, 1; 43-47
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The total protein content, protein fractions and proteases activities of drone prepupae of Apis mellifera due to varrosis
Autorzy:
Zoltowska, K
Lipinski, Z.
Dmitryjuk, M.
Powiązania:
https://bibliotekanauki.pl/articles/840749.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
protease
protease activity
honey bee
drone prepupa
protein fraction
varrosis
total protein
Varroa destructor
Apis mellifera
protein content
Opis:
The proteins level and activities of acid and alkaline proteases in whole body extracts of drone prepupae of Apis mellifera naturaly infested with Varroa destructor were studied. The infested and a non-infested group did not differ significantly in their total protein content. However, some differences in protein profiles were found. A lack of three protein fractions of moderate and lower molecular weight in infested prepupae was noted. Moreover, some differences in the quantity of protein in most of the fractions were observed. The activity of acid proteases from infested prepupae was lower (p < 0.05) compared with the activity of these proteases from the non-infested one group. The infested drone had higher activity of alkaline proteases than non-infested but this difference was not statisticaly significant.
Źródło:
Annals of Parasitology; 2005, 51, 1
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The properties and utilization of proteases of marine fish and invertebrates
Autorzy:
Kolodziejska, I.
Sikorski, Z.E.
Powiązania:
https://bibliotekanauki.pl/articles/1372735.pdf
Data publikacji:
1995
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
invertebrate
fish
protease
utilization
internal organ
thermal denaturation
textural change
metabolic function
muscle
marine organism
fish meat
marine animal
marine fish
life cycle
seafood
food preservation
property
protein concentrate
fish product
Opis:
The muscles and internal organs of marine animals contain many proteases. These enzymes fulfill different metabolic functions in the living organisms and affect the sensory quality and functional properties of the catch. Their activity depends on the species, life cycle, and the feeding status of the organism. Many proteases of marine origin differ from their counterparts in terrestrial animals in being more active at lower temperature and less resistant to thermal denaturation. Of practical importance in the industry is their role in ripening of salted fish, fish sauces and marinades, modyfying fish protein concentrates, ensilaging of seafood offal, and deskinning of fishery products. The negative effects comprise mainly the early post morten quality degradation of the catch and deterioration of the rheological properties of fish gels. Proteolytic preparations form different marine sources may be used successfully in food processing and as industrial enzymes, e. g. tanning agents.
Źródło:
Polish Journal of Food and Nutrition Sciences; 1995, 04, 1; 5-12
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of exogenous proteases on selected hematological and serum biochemical parameters in boars
Autorzy:
Zielonka, L
Przewoski, W.
Gajecka, M.
Jakimiuk, E.
Gajecki, M.
Powiązania:
https://bibliotekanauki.pl/articles/32369.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
boar
farm animal
pig
enzymatic complex
enzymatic growth promoter
hematological parameter
serum
biochemical parameter
proteolytic enzyme
physiological norm
nitrogen metabolism
Opis:
The increasing interest in enzymatic growth promoters prompted this investigation of the effect of an enzymatic stimulating complex on selected hematological and serum biochemical parameters in boars. The enzymatic complex [five proteases (proteinases-endopeptidases) and two peptidases (exopeptidases) obtained by fermentation from Streptomyces fradiae] was added to diets for three months, at initial doses of 90 (group El) and 120 (group E2) g/ton feed for the first seven days followed by 40 and 60 g/ton, respectively, in the subsequent weeks. The evaluation was based on selected hematological (RBC, WBC, HCT (Ht), HGB (Hb), MCV, MCH, and MCHC) and biochemical (AST, ALT, AP, Ca, Mg, urea, cholesterol, creatinine, inorganic phosphorus, and oxyglucose) parameters determined before and after the experimental period. The reported values were within physiological norms. Statistical differences were found between experimental groups with respect to WBC, HCT (Ht), MCV, MCH, AP, Ca, Mg, creatinine, and inorganic phosphorus. The study results indicate that the complex of proteolytic enzymes administered to boars caused no negative changes in their metabolic profiles. The statistically different hematological and serum biochemical parameters, while within normal limits, point to an early stage of microcytic anemia and heightened agitation that could be the result of intensified nitrogen metabolism.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 151-156
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The action of ten secreted aspartic proteases of pathogenic yeast Candida albicans on major human salivary antimicrobial peptide, histatin 5
Autorzy:
Bochenska, Oliwia
Rapala-Kozik, Maria
Wolak, Natalia
Aoki, Wataru
Ueda, Mitsuyoshi
Kozik, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1038755.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
secreted aspartic proteases
Sap
Candida albicans
antimicrobial peptides
histatin 5
candidiasis
Opis:
Candida albicans, belonging to the most common fungal pathogens of humans, exploits many virulence factors to infect the host, of which the most important is a family of ten secreted aspartic proteases (Saps) that cleave numerous peptides and proteins, often deregulating the host's biochemical homeostasis. It was recently shown that C. albicans cells can inactivate histatin5 (His5), a salivary histidine-rich anticandidal peptide, through the hydrolytic action of Saps. However, the current data on this subject are incomplete as only four out of ten Saps have been studied with respect to hydrolytic processing of His5 (Sap2, Sap5, Sap9-10). The aim of the study was to investigate the action of all Saps on His5 and to characterize this process in terms of peptide chemistry. It was shown that His5 was degraded by seven out of ten Saps (Sap1-4, Sap7-9) over a broad range of pH. The cleavage rate decreased in an order of Sap2>Sap9>Sap3>Sap7>Sap4>Sap1>Sap8. The degradation profiles for Sap2 and Sap9 were similar to those previously reported; however, in contrast to the previous study, Sap10 was shown to be unable to cleave His5. On a long-time scale, the peptide was completely degraded and lost its antimicrobial potential but after a short period of Sap treatment several shorter peptides (His1-13, His1-17, His1-21) that still decreased fungal survival were released. The results, presented hereby, provide extended characteristics of the action of C. albicans extracellular proteases on His5. Our study contribute to deepening the knowledge on the interactions between fungal pathogens and the human host.
Źródło:
Acta Biochimica Polonica; 2016, 63, 3; 403-410
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structure-function relationship of serine protease-protein inhibitor interaction.
Autorzy:
Otlewski, Jacek
Jaskólski, Mariusz
Buczek, Olga
Cierpicki, Tomasz
Czapińska, Honorata
Krowarsch, Daniel
Smalas, Arne
Stachowiak, Damian
Szpineta, Agnieszka
Dadlez, Michał
Powiązania:
https://bibliotekanauki.pl/articles/1044133.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
calorimetry
serine proteases
structural thermodynamics
protein inhibitor
protein-protein recognition
Opis:
We report our progress in understanding the structure-function relationship of the interaction between protein inhibitors and several serine proteases. Recently, we have determined high resolution solution structures of two inhibitors Apis mellifera chymotrypsin inhibitor-1 (AMCI-I) and Linum usitatissimum trypsin inhibitor (LUTI) in the free state and an ultra high resolution X-ray structure of BPTI. All three inhibitors, despite totally different scaffolds, contain a solvent exposed loop of similar conformation which is highly complementary to the enzyme active site. Isothermal calorimetry data show that the interaction between wild type BPTI and chymotrypsin is entropy driven and that the enthalpy component opposes complex formation. Our research is focused on extensive mutagenesis of the four positions from the protease binding loop of BPTI: P1, P1', P3, and P4. We mutated these residues to different amino acids and the variants were characterized by determination of the association constants, stability parameters and crystal structures of protease-inhibitor complexes. Accommodation of the P1 residue in the S1 pocket of four proteases: chymotrypsin, trypsin, neutrophil elastase and cathepsin G was probed with 18 P1 variants. High resolution X-ray structures of ten complexes between bovine trypsin and P1 variants of BPTI have been determined and compared with the cognate P1 Lys side chain. Mutations of the wild type Ala16 (P1') to larger side chains always caused a drop of the association constant. According to the crystal structure of the Leu16 BPTI-trypsin complex, introduction of the larger residue at the P1' position leads to steric conflicts in the vicinity of the mutation. Finally, mutations at the P4 site allowed an improvement of the association with several serine proteases involved in blood clotting. Conversely, introduction of Ser, Val, and Phe in place of Gly12 (P4) had invariably a destabilizing effect on the complex with these proteases.
Źródło:
Acta Biochimica Polonica; 2001, 48, 2; 419-428
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural studies of cysteine proteases and their inhibitors.
Autorzy:
Grzonka, Zbigniew
Jankowska, Elżbieta
Kasprzykowski, Franciszek
Kasprzykowska, Regina
Łankiewicz, Leszek
Wiczk, Wiesław
Wieczerzak, Ewa
Ciarkowski, Jerzy
Drabik, Piotr
Janowski, Robert
Kozak, Maciej
Jaskólski, Mariusz
Grubb, Anders
Powiązania:
https://bibliotekanauki.pl/articles/1044158.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cysteine proteases
structure-activity relationship
cystatins
synthetic inhibitors
Opis:
Cysteine proteases (CPs) are responsible for many biochemical processes occurring in living organisms and they have been implicated in the development and progression of several diseases that involve abnormal protein turnover. The activity of CPs is regulated among others by their specific inhibitors: cystatins. The main aim of this review is to discuss the structure-activity relationships of cysteine proteases and cystatins, as well as of some synthetic inhibitors of cysteine proteases structurally based on the binding fragments of cystatins.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 1-20
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Purification and characterization of the cuticle-degrading proteases produced by an isolate of Beauveria bassiana using the cuticle of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)
Autorzy:
Firouzbakht, H.
Zibaee, A.
Hoda, H.
Sohani, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/65065.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The entomopathogenic fungi-like Beauveria bassiana must penetrate via the integument of an insect to reach the hemocoel. Since proteins are the molecules responsible for integument strength in insects, the proteins must synthesise the cuticle degrading proteases which will then enable the proteases to penetrate. It is important to determine the biochemical properties of these proteases so that fungal virulence can be better understood. In the current study, a recently collected isolate of B. bassiana, namely AM-118, was inoculated in liquid media containing 0.5% of Andrallus spinidens Fabricus cuticle to obtain specific proteases. The crude samples were purified via a three step process using ammonium sulfate, Sepharyl G-100, and DEAE-Cellulose Fast Flow. The results revealed two proteases known as subtilisin-like (Pr1), and trypsin-like (Pr2), with the molecular weights of 105 and 103 kDa. The optimal pH and temperature values were found to be 8 and 35°C for Pr1 and 8 and 40°C for Pr2, respectively. Inhibitors like AEBSF, EDTA, TPCK, and phenanthroline significantly affected proteolytic activities. Here, we reported two fungal proteases by high molecular weight from an Iranian isolate of B. bassiana. These findings will help us to better understand fungal virulence against insects.
Źródło:
Journal of Plant Protection Research; 2015, 55, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteolytic activity of the viscera and partial purification of acid proteases of squid Illex argentinus hepatopancreas
Proteolityczna aktywnosc wnetrznosci i czesciowe oczyszczenie kwasnych proteaz z watrobo-trzustki kalmarow Illex argentinus
Autorzy:
Kolodziejska, I.
Mazurek, A.
Powiązania:
https://bibliotekanauki.pl/articles/1372840.pdf
Data publikacji:
1995
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
bialko
zywnosc
homogenaty
enzymy
stabilnosc
mrozenie
aktywnosc proteolityczna
kalmary
Illex argentinus
autoliza
autolizaty
utrwalanie zywnosci
watrobotrzustka
Opis:
The squid hepatopancreas, containing 20-60% lipids, is not suitable on board vessel for producing fodder meal and is often discarded, although it constitutes the major part of the viscera and is a rich source of proteolytic enzymes. The proteolytic activity against hemoglobin at pH 3.0 of the extract of the he pato pan ere a s of frozen stored squid, calculated per mg of protein, is about 20 times higher than that of the extract of the rest of the viscera. Due to incubation of the homogenate at pH 3.0 at 30 and 40°C in 2 days a clear watery phase was formed, well separated from the oily fraction containing floating solid particles of the autolysate. In 2 days about 35 and 50% of the protein was hydrolysed at 20°C and 30-40°C, respectively, to degradation products not precipitated by TCA. The proteolytic activity of the autolyzed homogenate after 2 days at pH 3.0 is about 80 % of that of the original homogenate. Incubation at 40°C at pH 3.0 decreased the activity after 6 days by about 50%. In the autolysate of squid hepatopancreas the proteolytic activity at pH 3.0 in the absence of reducing compounds was almost totally due to aspartic proteases. In the presence of dithiothreitol the activity increased by about 50%, but the increase was only partly due to the presence of thiol proteases.
Zbadano aktywność proteolityczną ekstraktów enzymów z wnętrzności mrożonych kalmarów Illex argentinus wobec hemoglobiny przy pH 3 oraz określono wpływ temperatury i czasu inkubacji na stabilność enzymów i autolizę białek homogenatu z wątrobo-trzustki. Proteolityczna aktywność przy pH 3 ekstraktu enzymów z wątrobo-trzustki kalmarów jest w przeliczeniu na 1 mg białek około 20 razy większa niż aktywność ekstraktów z pozostałych wnętrzności. Po dwóch dniach inkubacji homogenatu z wątrobo-trzustki w temperaturze 20°C i 30—40°C hydrolizuje odpowiednio około 35% i 50% białek. Proteolityczna aktywność w zautolizowanym homogenacie obniża się w tych warunkach o około 20%. Aktywność proteolityczna w autolizacie wykazywana przy pH 3 w nieobecności substancji redukujących pochodzi nieomal całkowicie od proteaz asparaginowych. Autoliza białek wątrobo-trzustki kalmarów jest prostą i tanią drogą uzyskiwania częściowo oczyszczonego preparatu enzymów proteolitycznych aktywnych w kwaśnym środowisku. Jednocześnie ułatwia ona oddzielenie lipidów i części stałych od klarownego autolizatu. Autolizat może stanowić półprodukt do otrzymywania preparatu enzymów proteolitycznych o większej czystości.
Źródło:
Polish Journal of Food and Nutrition Sciences; 1995, 04, 3; 63-70
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteazy: znaczenie, rola i oznaczanie
Proteases: significance, role and determination
Autorzy:
Tokarzewicz, A
Gorodkiewicz, E.
Powiązania:
https://bibliotekanauki.pl/articles/143075.pdf
Data publikacji:
2015
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
proteazy
proteinazy
peptydazy
enzymy proteolityczne
nowotwór
proteases
proteinases
peptidases
proteolytic enzymes
cancer
Opis:
Proteazy są związkami, które odgrywają bardzo istotną rolę w ludzkim organizmie. Regulują one wiele procesów przebiegających także w innych żywych organizmach, również w wirusach, bakteriach i pasożytach. Ich zwiększona lub obniżona ilość może wskazywać na nieprawidłowości w orgaznizmie, takie jak: rozwój zapalenia, nowotwory, nadciśnienie. W artykule opisano najistotniejsze z ludzkich proteaz, ich znaczenie, rolę w organizmie oraz pokrótce metody oznaczania.
Proteases are compounds that play an important role in the human body. They regulate many processes inside the living organisms, also in viruses, bacteria and parasites. Their increased or decreased level may indicate irregularities in the body, such as: the development of inflammation, cancer and others like hypertension. This article describes the most important of human proteases, their significance, roles in the organism and shortly methods of determination.
Źródło:
Chemik; 2015, 69, 2; 81-88
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
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