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Tytuł:
New proteases of the chloroplast envelope – what do they do there?
Autorzy:
Adam, Z.
Powiązania:
https://bibliotekanauki.pl/articles/80681.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
protease
chloroplast
thylakoid
proteomics
allene oxide synthase
jasmonic acid
serine protease
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protease Inhibitor from the Crude Extract of Plant Seeds Affects the Digestive Proteases in Hyphantria Cunea (Lep.: Arctiidae)
Autorzy:
Aghaali, N.
Ghadamyari, M.
Hosseininaveh, V.
Riseh, N.S.
Powiązania:
https://bibliotekanauki.pl/articles/66524.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Proteases are one of the most important digestive enzymes in the midgut of Hyphantria cunea Drury. Proteases are responsible for protein digestion. In the present study, we evaluated the efficiency of some plant inhibitors on proteases in the gut of the H. cunea. Last instar larvae were collected from mulberry trees. The digestive system of the larvae was used as an enzyme source. The total proteolytic and trypsin activity were assessed by the hemoglobin and BApNA, respectively, as the substrate. The evaluation of the total proteolytic and trypsin activities in various pHs showed the highest relative activity at a pH of 11. Also, the inhibitory effect of inhibitors extracted from Alhagi maurorum Medik., Lathyrus sativus L., Vicia faba L., Prosopis farcta (Banks & Sol.) Eig., and Panicum miliaceum L. on the digestive protease of the fall webworm was measured. Protease inhibitors extracted from A. maurorum, P. farcta and P. miliaceum showed negligible inhibition but L. sativus was able to inhibit 34.72% and 100% of the total activity of proteolytic and trypsin, respectively. Also, the total proteolytic and trypsin activities were inhibited by the inhibitor from V. faba, at 22.27% and 100%, respectively. The zymogram pattern of trypsin with nitro-cellulose membranes showed 2 isoforms in the gut of H. cunea. The inhibitor from L. sativus completely inhibited both isoforms. Gel electrophoresis of proteolitytic activity revealed at least 6 isoforms the inhibitor extracted from L. sativus; completely inhibiting some of them. The inhibitor from L. sativus was purified by ammonium sulfate precipitation and gel-filtration. The molecular mass of the inhibitor was determined as 45 kDa. The highest inhibition of trypsin activity by the inhibitor from L. sativus occurred at a pH of 10. The stability of the inhibitor from L. sativus was evaluated at different pHs and temperatures. The results showed that the inhibitor from L. sativus was stable at a pH of 11.0, and showed 45% inhibition on trypsin activity at a pH of 11. Also, this inhibitor revealed stability up to 50°C.
Źródło:
Journal of Plant Protection Research; 2013, 53, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Three Pseudomonas aeruginosa strains with different protease profiles
Autorzy:
Andrejko, Mariola
Zdybicka-Barabas, Agnieszka
Janczarek, Monika
Cytryńska, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1039614.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aprA
alkaline protease
extracellular proteases
elastase B
virulence
lasB
Pseudomonas aeruginosa
Opis:
The proteolytic activity of three Pseudomonas aeruginosa strains, ATCC 27853 - a reference strain, and two clinical isolates was tested. The activity was examined after culturing the bacteria in two different growth media: the minimal M9 medium and rich Luria-Bertani broth (LB). Based on zymograms and protease activity specific assays, it was concluded that the reference strain produced three proteolytic enzymes in the LB medium: protease IV, elastase B and elastase A, while alkaline protease was only produced in the M9 medium. The clinical isolates of P. aeruginosa produced elastase B and alkaline protease when grown in the LB medium and the minimal M9 medium, respectively. PCR analysis confirmed the presence of both the lasB gene encoding elastase B and aprA coding for alkaline protease in the genomes of the three P. aeruginosa strains analyzed. The expression of these genes coding for two important P. aeruginosa virulence factors was dependent on the growth conditions in all the strains studied. The contribution of the extracellular proteinases to the virulence of P. aeruginosa strains used in this study was investigated using an insect model, the greater wax moth Galleria mellonella.
Źródło:
Acta Biochimica Polonica; 2013, 60, 1; 83-90
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The action of ten secreted aspartic proteases of pathogenic yeast Candida albicans on major human salivary antimicrobial peptide, histatin 5
Autorzy:
Bochenska, Oliwia
Rapala-Kozik, Maria
Wolak, Natalia
Aoki, Wataru
Ueda, Mitsuyoshi
Kozik, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1038755.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
secreted aspartic proteases
Sap
Candida albicans
antimicrobial peptides
histatin 5
candidiasis
Opis:
Candida albicans, belonging to the most common fungal pathogens of humans, exploits many virulence factors to infect the host, of which the most important is a family of ten secreted aspartic proteases (Saps) that cleave numerous peptides and proteins, often deregulating the host's biochemical homeostasis. It was recently shown that C. albicans cells can inactivate histatin5 (His5), a salivary histidine-rich anticandidal peptide, through the hydrolytic action of Saps. However, the current data on this subject are incomplete as only four out of ten Saps have been studied with respect to hydrolytic processing of His5 (Sap2, Sap5, Sap9-10). The aim of the study was to investigate the action of all Saps on His5 and to characterize this process in terms of peptide chemistry. It was shown that His5 was degraded by seven out of ten Saps (Sap1-4, Sap7-9) over a broad range of pH. The cleavage rate decreased in an order of Sap2>Sap9>Sap3>Sap7>Sap4>Sap1>Sap8. The degradation profiles for Sap2 and Sap9 were similar to those previously reported; however, in contrast to the previous study, Sap10 was shown to be unable to cleave His5. On a long-time scale, the peptide was completely degraded and lost its antimicrobial potential but after a short period of Sap treatment several shorter peptides (His1-13, His1-17, His1-21) that still decreased fungal survival were released. The results, presented hereby, provide extended characteristics of the action of C. albicans extracellular proteases on His5. Our study contribute to deepening the knowledge on the interactions between fungal pathogens and the human host.
Źródło:
Acta Biochimica Polonica; 2016, 63, 3; 403-410
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Alzheimers disease: Its origin at the membrane, evidence and questions.
Autorzy:
Buchet, Rene
Pikuła, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/1044315.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
β-amyloid precursor protein
membrane proteases
Alzheimer's disease
lipid composition
membrane microdomains
Opis:
Numerous results on membrane lipid composition from different regions of autopsied Alzheimer's disease brains in comparison with corresponding fractions isolated from control brains revealed significant differences in serine- and ethanolamine-containing glycerophospholipid as well as in glycosphingolipid content. Changes in membrane lipid composition are frequently accompanied by alterations in membrane fluidity, hydrophobic mismatch, lipid signaling pathways, transient formation and disappearance of lipid microdomains, changes in membrane permeability to cations and variations of other membrane properties. In this review we focus on possible implications of altered membrane composition on β-amyloid precursor protein (APP) and on proteolysis of APP leading eventually to the formation of neurotoxic β-amyloid (Aβ) peptides, the major proteinaceous component of extracellular senile plaques, directly involved in Alzheimer's disease pathogenesis.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 725-733
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Theoretical studies of binding modes of two covalent inhibitors of cysteine proteases.
Autorzy:
Drabik, Piotr
Politowska, Ewa
Czaplewski, Cezary
Kasprzykowski, Franciszek
Łankiewicz, Leszek
Ciarkowski, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1044228.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cysteine proteases
covalent protease inhibitors
constrained simulated annealing
papain
molecular dynamics
Opis:
Physiological and pathological roles of cysteine proteases make them important targets for inhibitor development. Although highly potent inhibitors of this group of enzymes are known, their major drawback is a lack of sufficient specificity. Two cysteine protease covalent inhibitors, viz. (i) Z-RL-deoxo-V-peptide-epoxysuccinyl hybrid, and (ii) Z-RLVG-methyl-, have been developed and modeled in the catalytic pocket of papain, an archetypal thiol protease. A number of configurations have been generated and relaxed for each system using the AMBER force field. The catalytic pockets S3 and S4 appear rather elusive in view of the observed inhibitors' flexibility. This suggest rather limited chances for the development of selective structure-based inhibitors of thiol proteases, designed to exploit differences in the structure of catalytic pockets of various members of this family.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 1061-1066
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Defense against own arms: staphylococcal cysteine proteases and their inhibitors.
Autorzy:
Dubin, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1043443.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
staphostatin
staphylococcus
ssp
proteinase
protease inhibitor
staphopain
Opis:
Staphylococcus aureus is a human pathogen causing a wide range of diseases. Most staphylococcal infections, unlike those caused by other bacteria are not toxigenic and very little is known about their pathogenesis. It has been proposed that a core of secreted proteins common to many infectious strains is responsible for colonization and infection. Among those proteins several proteases are present and over the years many different functions in the infection process have been attributed to them. However, little direct, in vivo data has been presented. Two cysteine proteases, staphopain A (ScpA) and staphopain B (SspB) are important members of this group of enzymes. Recently, two cysteine protease inhibitors, staphostatin A and staphostatin B (ScpB and SspC, respectively) were described in S. aureus shedding new light on the complexity of the processes involving the two proteases. The scope of this review is to summarize current knowledge on the network of staphylococcal cysteine proteases and their inhibitors in view of their possible role as virulence factors.
Źródło:
Acta Biochimica Polonica; 2003, 50, 3; 715-724
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions
Autorzy:
Dziekonska-Rynko, J
Rokicki, J.
Jablonowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/837165.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
protease activity
stage
papain
pepsin
trypsin
larva
Anisakis simplex
in vitro
Opis:
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
Źródło:
Annals of Parasitology; 2002, 48, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions
Autorzy:
Dziekońska-Rynko, J.
Rokicki, J.
Jabłonowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2147699.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
protease activity
stage
papain
pepsin
trypsin
larva
Anisakis simplex
in vitro
Opis:
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
Źródło:
Wiadomości Parazytologiczne; 2002, 48, 2; 217-223
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ zakażenia larwami III stadium Anisakis simplex na aktywność proteaz w przewodzie pokarmowym świnek morskich
THE INFLUENCE OF INFECTION WITH III LARVAL STAGE OF ANISAKIS SIMPLEX ON THE ACTIVITY OF PROTEASES IN ALIMENTARY TRACT OF GUINEA PIGS*
Autorzy:
Dziekońska-Rynko, J.
Rokicki, J.
Jabłonowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2148919.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przewod pokarmowy
enzymy proteolityczne
Anisakis simplex
aktywnosc enzymatyczna
parazytologia
inwazja pasozytnicza
proteaza
swinki morskie
larwy
Opis:
The studies were carried out on guinea pig males. The animals were infected with 30 larvae (L3) of Anisakis simplex. After 6 hrs of invasion the animals were dissected. In homogenized pancreas and duodenal contents activities of trypsin were determined. In stomach content activities of pepsin were determined. The activities of trypsin in duodenal contents and in pancreas homogenate from infected animals were lower in comparison with the control animals. The activities of pepsin were higher in infected animals.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 4; 425-430
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of microbial proteases to obtain egg-yolk protein hydrolysates with antioxidant and antimicrobial activity
Zastosowanie proteaz pochodzenia mikrobiologicznego do otrzymania hydrolizatów o aktywności przeciwutleniającej i przeciwdrobnoustrojowej z białek żółtka jaja
Autorzy:
Eckert, E.
Zambrowicz, A.
Pokora, M.
Dabrowska, A.
Szoltysik, M.
Chrzanowska, J.
Trziszka, T.
Powiązania:
https://bibliotekanauki.pl/articles/828454.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Technologów Żywności
Opis:
Natural antioxidants with high safety and long-term effect are subject of many studies because they are an alternative to chemical antioxidants which, in larger quantities, can be toxic. One type of natural antioxidants may be food- derived peptides. The aim of this study was to obtain antioxidant and antimicrobial peptides from egg yolk phosvitin and immunoglobulin Y (IgY) with participation of microbial proteinases from Bacillus amyloliquefaciens (neutrase), B. thermoproteolyticus Rokko (thermolysin), Streptomyces griseus (pronase) and Aspergillus melleus. The progress of hydrolysis was monitored by the degree of hydrolysis (DH) and free amino groups concentration measurement. The resulting hydrolysates were subjected to an assessment of their ability to reduce the oxidation state of metal ions, scavenging of 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) free radicals and chelating iron ions. The highest degree of hydrolysis of both proteins was obtained during the reaction with proteinase from S. griseus. The highest level of reduction in the oxidation state of iron ions was observed in IgY 24-hour hydrolysates obtained with the participation of enzyme from B. thermoproteolyticus Rokko (409.7 μg Fe²+/mg). However, the 24-hour hydrolysates of IgY obtained after degradation with the proteinase from A. melleus, possessed the highest free radical scavenging activity equal to1.46 μM trolox/mg. The highest activity of chelating iron ions, equal to 891.64 μg Fe²+/mg, was observed for products obtained during the 24-hour hydrolysis of phosvitin with the participation of protease from B. thermoproteolyticus Rokko.
Przeciwutleniacze naturalne są obiektem wielu badań ze względu na długotrwałą efektywność i bezpieczeństwo stosowania. Mogą one stanowić alternatywę syntetycznych przeciwutleniaczy, które są toksyczne dla organizmu. Do naturalnych przeciwutleniaczy zalicza się m.in. peptydy pochodzące z białek żywnościowych. Celem pracy było otrzymanie peptydów o aktywności przeciwutleniającej i przeciwdrobnoustrojowej z foswityny i immunoglobuliny Y z wykorzystaniem enzymów proteolitycznych pochodzenia mikrobiologicznego, takich jak: proteaza z Bacillus amyloliquefaciens (neutraza), proteaza z B. thermoproteolyticus Rokko (termolizyna), proteaza ze Streptomyces griseus (pronaza) i proteaza z Aspergillus melleus. Postęp hydrolizy monitorowano przez oznaczenie stopnia hydrolizy (DH) i stężenia wolnych grup aminowych. Aktywność przeciwutleniającą otrzymanych hydrolizatów oznaczano jako zdolność do: redukcji stopnia utlenienia jonów żelaza, wygaszania wolnych rodników 2,2 difenylo-1-pikrylhydrazylowych i chelatowania jonów żelaza(II). Zarówno foswityna, jak i IgY wykazały najwyższą podatność na działanie proteazy ze S. griseus (pronazy). Hydrolizat IgY otrzymany w wyniku 24-godzinnej reakcji z proteazą z B. thermoproteolyticus Rokko (neutrazą) charakteryzował się największą zdolnością redukującą (409,7 μg Fe²+/mg). Natomiast najwyższy poziom zdolności wygaszania wolnych rodników DPPH, wynoszący: 1,46 μM trolox/mg uzyskano dla 24-godzinnego hydrolizatu IgY otrzymanego z udziałem proteazy z A. melleus. Największą zdolność do chelatowania jonów żelaza(II) wykazał hydrolizat uzyskany w wyniku 24-godzinnego działania proteazy z B. thermoproteolyticus Rokko na foswitynę (891,64 μg Fe²+/mg).
Źródło:
Żywność Nauka Technologia Jakość; 2013, 20, 1
1425-6959
Pojawia się w:
Żywność Nauka Technologia Jakość
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Purification and characterization of the cuticle-degrading proteases produced by an isolate of Beauveria bassiana using the cuticle of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)
Autorzy:
Firouzbakht, H.
Zibaee, A.
Hoda, H.
Sohani, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/65065.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The entomopathogenic fungi-like Beauveria bassiana must penetrate via the integument of an insect to reach the hemocoel. Since proteins are the molecules responsible for integument strength in insects, the proteins must synthesise the cuticle degrading proteases which will then enable the proteases to penetrate. It is important to determine the biochemical properties of these proteases so that fungal virulence can be better understood. In the current study, a recently collected isolate of B. bassiana, namely AM-118, was inoculated in liquid media containing 0.5% of Andrallus spinidens Fabricus cuticle to obtain specific proteases. The crude samples were purified via a three step process using ammonium sulfate, Sepharyl G-100, and DEAE-Cellulose Fast Flow. The results revealed two proteases known as subtilisin-like (Pr1), and trypsin-like (Pr2), with the molecular weights of 105 and 103 kDa. The optimal pH and temperature values were found to be 8 and 35°C for Pr1 and 8 and 40°C for Pr2, respectively. Inhibitors like AEBSF, EDTA, TPCK, and phenanthroline significantly affected proteolytic activities. Here, we reported two fungal proteases by high molecular weight from an Iranian isolate of B. bassiana. These findings will help us to better understand fungal virulence against insects.
Źródło:
Journal of Plant Protection Research; 2015, 55, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inactivation of α1-proteinase inhibitor by Candida albicans aspartic proteases favors the epithelial and endothelial cell colonization in the presence of neutrophil extracellular traps
Autorzy:
Gogol, Mariusz
Ostrowska, Dominika
Klaga, Kinga
Bochenska, Oliwia
Wolak, Natalia
Aoki, Wataru
Ueda, Mitsuyoshi
Kozik, Andrzej
Rapala-Kozik, Maria
Powiązania:
https://bibliotekanauki.pl/articles/1038860.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Candida albicans
aspartic proteases
α1-proteinase inhibitor
elastase
neutrophil extracellular traps
inflammation
Opis:
Candida albicans, a causative agent of opportunistic fungal infections in immunocompromised patients, uses ten secreted aspartic proteases (SAPs) to deregulate the homeostasis of the host organism on many levels. One of these deregulation mechanisms involves a SAP-dependent disturbance of the control over proteolytic enzymes of the host by a system of dedicated proteinase inhibitors, with one important example being the neutrophil elastase and alpha1-proteinase inhibitor (A1PI). In this study, we found that soluble SAPs 1-4 and the cell membrane-anchored SAP9 efficiently cleaved A1PI, with the major cleavage points located at the C-terminal part of A1PI in a close vicinity to the reactive-site loop that plays a critical role in the inhibition mechanism. Elastase is released by neutrophils to the environment during fungal infection through two major processes, a degranulation or formation of neutrophil extracellular traps (NET). Both, free and NET-embedded elastase forms, were found to be controlled by A1PI. A local acidosis, resulting from the neutrophil activity at the infection sites, favors A1PI degradation by SAPs. The deregulation of NET-connected elastase affected a NET-dependent damage of epithelial and endothelial cells, resulting in the increased susceptibility of these host cells to candidal colonization. Moreover, the SAP-catalyzed cleavage of A1PI was found to decrease its binding affinity to a proinflammatory cytokine, interleukin-8. The findings presented here suggest a novel strategy used by C. albicans for the colonization of host tissues and overcoming the host defense.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 167-175
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural studies of cysteine proteases and their inhibitors.
Autorzy:
Grzonka, Zbigniew
Jankowska, Elżbieta
Kasprzykowski, Franciszek
Kasprzykowska, Regina
Łankiewicz, Leszek
Wiczk, Wiesław
Wieczerzak, Ewa
Ciarkowski, Jerzy
Drabik, Piotr
Janowski, Robert
Kozak, Maciej
Jaskólski, Mariusz
Grubb, Anders
Powiązania:
https://bibliotekanauki.pl/articles/1044158.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cysteine proteases
structure-activity relationship
cystatins
synthetic inhibitors
Opis:
Cysteine proteases (CPs) are responsible for many biochemical processes occurring in living organisms and they have been implicated in the development and progression of several diseases that involve abnormal protein turnover. The activity of CPs is regulated among others by their specific inhibitors: cystatins. The main aim of this review is to discuss the structure-activity relationships of cysteine proteases and cystatins, as well as of some synthetic inhibitors of cysteine proteases structurally based on the binding fragments of cystatins.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 1-20
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Partial characterization of proteases from Citrus sinensis fruit peel
Autorzy:
Ibraheem, Ademola Saheed
Malomo, Silvia O.
Powiązania:
https://bibliotekanauki.pl/articles/1178685.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Citrus sinensis
Proteases
industries
kinetic parameters
Opis:
Proteases are one of the most important enzymes that have various physiological and industrial applications. This study was carried out to purify and partially characterize proteases from Citrus sinensis fruit peel. Three active fractions of the proteases (I, II and III) were obtained. The Vmax for proteases I, II, III and pooled fraction were 185.19, 192.31, 111.11 and 163.93 U/ml with Michaelis-Menten’s constant (Km) 1.01, 0.44, 0.67 and 0.37 mg/ml respectively. The enzymes were optimally active at 40-50 °C. However, they retained activity at 60-70 °C. Protease I was stable up to 60 °C while proteases II and III retained more than 80% activity in the range of 25-70 °C. The optimal pH of proteases II and III was 7 while protease I was optimally active at pH 8. The enzymes were stable at alkaline pH especially between 6 and 9 retaining more than 60% of its activity. The stability of these enzymes at high temperature and different pH may be an indication of its potential applications in food, chemical and laundry industries.
Źródło:
World Scientific News; 2017, 67, 2; 250-264
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 43

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