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Tytuł:
A study on influence of different signs of air-ions on sister-chromatid exchange frequency and chromosome aberration in human peripheral blood lymphocytes
Autorzy:
Wiszniewski, A
Kretowicz, T.
Powiązania:
https://bibliotekanauki.pl/articles/2043653.pdf
Data publikacji:
1999
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
blood lymphocyte
sister-chromatid exchange
man
peripheral blood lymphocyte
lymphocyte
chromosome aberration
Źródło:
Journal of Applied Genetics; 1999, 40, 2; 129-134
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in phosphorylation of histone H2A.X and p53 in response of peripheral blood lymphocytes to gamma irradiation
Autorzy:
Vilasová, Zdeňka
Řezáčová, Martina
Vávrová, Jiřina
Tichý, Aleš
Vokurková, Doris
Zoelzer, Friedo
Řeháková, Zuzana
Osterreicher, Jan
Lukášová, Emilie
Powiązania:
https://bibliotekanauki.pl/articles/1040760.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lymphocyte
ionizing radiation
p53
phytohemagglutinin (PHA)
apoptosis
DNA damage
Opis:
The main aim of this study was to compare the reaction of quiescent and proliferating, i.e. phytohemagglutinin (PHA)-stimulated, human peripheral blood mononuclear cells (PBMCs) to γ-radiation, and analyse changes of proteins related to repair of DNA damage and apoptosis, such as γH2A.X, p53, p53 phosphorylation at serines-15 and -392, and p21 and their dose dependence. Freshly isolated PBMCs in peripheral blood are predominantly quiescent, in G0 phase, and with very low amounts of proteins p53 and p21. Using confocal microscopy we detected dose dependent (0.5-5 Gy) induction of foci containing γH2A.X (1 h after γ-ray exposure), which are formed around radiation-induced double strand breaks of DNA. Apoptosis was detected from 24 h after irradiation by the dose of 4 Gy onwards by Annexin V binding and lamin B cleavage. Seventy two hours after irradiation 70% of CD3+ lymphocytes were A+. Neither increase in p53 nor its phosphorylation on serine-392 after irradiation was detected in these cells. However, massive increase in p21 (cyclin-dependent kinase inhibitor 1A) was detected after irradiation, which can be responsible for late occurrence of apoptosis in these quiescent cells. PHA-stimulation itself (72 h) caused an increase in early apoptosis (A+PI-) in comparison to non-stimulated PBMCs (38% A+ resp. 13.4%). After PHA-stimulation also the amount of γH2A.X, p53, and p21 increased, but no phosphorylation of p53 on serine-392 or -15 was detected. Reaction to γ-radiation was different in PHA-stimulated lymphocytes: the p53 pathway was activated and p53 was phosphorylated on serines-15 and -392 4 h after irradiation by the dose of 4 Gy. Phosphorylation of p53 at serine-15 increased in a dose-dependent manner in the studied dose range 0.2-7.5 Gy. Also the amount of p21 increased after irradiation. Seventy two hours after irradiation of PHA-stimulated CD3+ T lymphocytes by the dose of 4 Gy 65% of cells were A+.
Źródło:
Acta Biochimica Polonica; 2008, 55, 2; 381-390
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modelling the frequencies of chromosomal aberrations in peripheral lymphocytes of patients undergoing radiotherapy
Autorzy:
Urbanik, W.
Kukołowicz, P.
Kuszewski, T.
Góźdź, S.
Wójcik, A.
Powiązania:
https://bibliotekanauki.pl/articles/148614.pdf
Data publikacji:
2003
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
biological dosimetry
chromosomal aberration
peripheral lymphocytes
radiotherapy
Opis:
Recently, several attempts have been undertaken to correlate in vitro calibration curves with observed frequencies of chromosomal aberrations and micronuclei in lymphocytes of patients undergoing radiotherapy. The aim of such correlations is the search for a biological method to reconstruct the dose received during radiotherapy. While the in vitro dose-response curves are linear-quadratic, the observed in vivo relationship is usually linear and there is some controversy as to the nature of the observed linearity. We have, therefore, constructed a model to calculate the frequencies and distributions of chromosomal aberrations in lymphocytes of patients undergoing conventional radiotherapy. The model assumes that each fraction of radiation induces a certain number of Poisson-distributed aberrations in the irradiated blood volume. In addition, a simplified assumption is made that lymphocytes flow freely inside the body of the patient and no elimination of cells occurs. The model yields linear dose response curves. The steepness of the curves increases with increasing size of irradiated block of tissue (referred to as irradiated volume) and increasing dose per fraction. The distributions of aberrations become increasingly overdispersed with increasing dose per fraction but are independent of the number of radiation fractions. The modelled dose-response curves agree well with the majority of published experimental results. Given the simple assumptions made, this indicates that cell elimination, which occurs during radiotherapy does not bias the results obtained experimentally. The linearity of the dose response-curve results from the fractionated irradiation. Hence, great care should be applied when attempting to use standard, linear-quadratic calibration curves to estimate the doses received by patients during radiotherapy.
Źródło:
Nukleonika; 2003, 48, 1; 3-8
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Values of apoptosis of lymphocytes and granulocytes in peripheral blood of Polish mixed-breed rabbits in the annual cycle
Autorzy:
Tokarz-Deptula, D.
Hukowska-Szematowicz, B.
Niedzwidzka-Rystwej, P.
Trzeciak-Ryczek, A.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/31009.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of the study was to determine values of apoptosis for peripheral blood lymphocytes and granulocytes, including dependency on the season of the year, in Polish mixed-breed rabbits and in mixed-breed rabbits with the addition of blood of meat-breed rabbits. The study was carried out in four seasons of the year (spring, summer, autumn, winter) involving 120 Polish mixed-breed rabbits – group I, and 120 Polish mixed-breed rabbits with addition of meat-breed rabbit blood – group II. Assessment of apoptosis of lymphocytes and granulocytes was performed using as FACScan cytometer by Becton Dickinson with FACSDiva software (USA), using as ApoFluor®Green Caspase reagent kit (MP Biomedicals, USA) to detect the activity of the total caspase pool 1, 3, 4, 5, 6, 7, 8, 9 in granulocytes and lymphocytes of rabbit peripheral blood. The results for apoptosis of lymphocytes and granulocytes in peripheral blood in the animals investigated (group I and II) were subjected to statistical analysis with the t’Student test at p=0.05. It was noted that, in rabbits from group I, the values for apoptosis of lymphocytes were the highest in winter and autumn (36.02% and 31.24%, respectively), and the lowest in spring and summer (26.73% and 22.72%, respectively), whereas in the case of granulocytes the highest values were in summer and spring (14.69% and 12.95%, respectively), and the lowest in winter and autumn (8.16% and 8.57%, respectively). In mixed-breed rabbits with the addition of meat-breed blood (group II), the values for apoptosis of lympocytes were the highest in spring (29.13%), and the lowest in summer (25.43%); whereas in the case of granulocytes the highest values were in summer and spring (14.0% and 11.15%, respectively), and the lowest in autumn and winter (7.46% and 7.64%, respectively).
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Values of B and T cells and their subpopulations in peripheral blood of rabbits
Autorzy:
Tokarz-Deptuła, Beata
Niedźwiedzka-Rystwej, Paulina
Trzeciak-Ryczek, Alicja
Pępiak, Andrzej
Deptuła, Wiesław
Powiązania:
https://bibliotekanauki.pl/articles/1386044.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
rabbit
B and T lymphocytes
reference values
królik
limfocyty B i T
wartości referencyjne
Opis:
Determining the reference values for B and T lymphocytes and their subpopulations in rabbits used in Poland for economic and laboratory purposes is not very common. The goal of this study was to evaluate values of chosen B and T cells and their subpopulations in Polish mixed-breed rabbits, taking season of the year and sex of the animals into the consideration. The study was performed with the use of flow cytometry using monoclonal antibodies (mouse anti-rabbit). The study revealed that, both in Polish mixed-breed rabbits and in Polish mixed-breed rabbits with an addition of blood of meat breeds, the season of the year and sex of the animals affectthe percentage of B- and T-cells and their subpopulations in peripheral blood. As far as season of the year is concerned, it was shown that it mainly affects CD5+ T cells in mixed-breed rabbits and CD25+ T cells in mixed-breed rabbits with an addition of blood of meat breed, while taking sex of the animals into account, more changes were recorded in females in both groups of rabbits. Due to the fact that this is the first paper on those values in Polish rabbits conducted on a large amount of animals, the values may be treated as reference.
Ocena wartości referencyjnych dla limfocytów B i T oraz ich subpopulacji u królików wykorzystywanych w Polsce w celach ekonomicznych i laboratoryjnych jest rzadkością. Celem pracy jest ocena parametrów wybranych limfocytów B i T oraz ich subpopulacji u polskich królików mieszańców z uwzględnieniem pory roku i płci tych zwierząt. Badania wykonano z wykorzystaniem cytometrii przepływowej i przeciwciał monoklonalnych (mouse anti-rabbit). Badania wykazały, że zarówno u królików mieszańców polskich, jak i mieszańców polskich z dolewem krwi ras mięsnych, pora roku i płeć wpływa na kształtowanie badanych parametrów, tj. procentu limfocytów B i T oraz ich subpopulacji. Co się tyczy pory roku wykazano, że zmiany dotyczą głównie limfocytów T CD5+ w przypadku królików mieszańców polskich i limfocytów T CD25+ w przypadku królików mieszańców polskich z dolewem krwi ras mięsnych. Tymczasem w przypadku płci zwierząt, więcej zmian w obu grupach zwierząt wykazano u samic. Ze względu na to, że jest to pierwsza praca z tego zakresu i badania dotyczą dużej grupy zwierząt, winny one być traktowane jako wartości referencyjne.
Źródło:
Acta Biologica; 2016, 23; 127-138
2450-8330
2353-3013
Pojawia się w:
Acta Biologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metoda mikroanalizy rentgenowskiej w badaniach składu chemicznego limfocytów krwi obwodowej
Metod rentgenovskogo mikroanaliza v issledovanijakh khimicheskogo sostava limfocitov perifericheskojj krovi
X-ray microanalysis in examinations of chemical constitution of peripheral blood lymphocytes
Autorzy:
Pilch, J.
Łaskawiec, J.
Lisiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/2186393.pdf
Data publikacji:
1988
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Tematy:
diagnostyka laboratoryjna
badania krwi
krew obwodowa
limfocyty
sklad chemiczny
zawartosc pierwiastkow sladowych
mikroanaliza rentgenowska
Źródło:
Diagnostyka Laboratoryjna; 1988, 24, 6; 295-304
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Apoptosis of peripheral blood leucocytes in rabbits infected with different strains of rabbit haemorrhagic disease virus
Autorzy:
Niedźwiedzka-Rystwej, Paulina
Hukowska-Szematowicz, Beata
Tokarz-Deptuła, Beata
Trzeciak-Ryczek, Alicja
Działo, Joanna
Deptuła, Wiesław
Powiązania:
https://bibliotekanauki.pl/articles/1039609.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
granulocytes
RHDV
lymphocytes
apoptosis
RHDVa
Opis:
The pathogenicity of RHDV (rabbit haemorrhagic disease virus) is mainly associated with its affinity to blood vessels, with causing disseminated intravascular coagulations (DIC), and with the stimulation of the host immune system. Moreover, there are implications suggesting that apoptosis may be a pivotal process in understanding the basis of viral haemorrhagic disease in rabbits - a serious infectious disease causing mortality to wild and domestic rabbits. The aim of this study is to evaluate, by means of flow cytometry, the dynamics of apoptosis in peripheral blood granulocytes and lymphocytes in rabbits experimentally infected with seven different strains of RHDV and so-called antigenic variants of RHDV denominated as RHDVa, i.e.: Hungarian 24V/89, 1447V/96, 72V/2003; Austrian 01-04, 237/04, V-412 and French 05-01. The results showed that all of the RHDV and RHDVa strains cause an increase in the number of apoptotic cells throughout the infection, which might indicate the need for further analysis of the importance of this process.
Źródło:
Acta Biochimica Polonica; 2013, 60, 1; 65-69
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating and non-haemagglutinating antigenic variants of the RHD (rabbit haemorrhagic disease) virus
Autorzy:
Niedzwiedzka-Rystwej, P.
Tokarz-Deptula, B.
Deptula, W.
Powiązania:
https://bibliotekanauki.pl/articles/30375.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
This paper attempts to study the dynamics of apoptosis of granulocytes and lymphocytes in peripheral blood in rabbits infected with haemagglutinating (Vt97, Triptis, Hartmannsdorf) and non-haemagglutinating (Pv97, 9905 RHDVa) antigenic variants of the RHD virus. The pathogenicity of those antigenic variants was also assessed by recording the mortality of the infected animals. The animals were infected with antigenic variants and blood was sampled at hour 0,4,8,12,24,36 p.i. and the percentage of apoptotic granulocytes and lymphocytes was measured with the use of flow cytometry. The results of the study showed that apoptosis is included during RHDV infection, as the number of apoptotic granulocytes and lymphocytes increases throughout the experiment; depending on the antigenic variant, apoptosis joins in at 4-8-12 h p.i. and lasts until 24-36 h p.i. Furthermore, the mortality of rabbits infected with the examined strains of RHD virus varied from 30% to 100%. This study performed for the first time in this manner, indicates the importance of apoptosis during infection with the RHD virus.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metody fluorescencyjnej do oceny lizosomów limfocytów krwi obwodowej królika
Primenenie fluorescentnogo metoda k ocenke lizosom limfocitov perifericheskojj krovi krolikov
The application of fluorescent method in evaluation of lysosomes in peripheral blood lymphocytes of rabbit
Autorzy:
Mdzewski, B.
Grabarczyk, M.
Sokołowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/2189117.pdf
Data publikacji:
1976
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1976, 12, 5; 293-298
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
Autorzy:
Kopjar, Nevenka
Želježić, Davor
Garaj-Vrhovac, Verica
Powiązania:
https://bibliotekanauki.pl/articles/1041246.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
peripheral blood
chromosome aberration test
white blood cells
DNA damage
alkaline comet assay
lymphocytes
Opis:
The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 321-336
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Arginase activity alterations in peripheral blood lymphocytes in the human chronic lymphocytic leukemia
Autorzy:
Konarska, Liliana
Widzyńska, Iwona
Zienkiewicz, Halina
Sułek, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1045547.pdf
Data publikacji:
1993
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1993, 40, 1; 160-163
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prosta metoda izolowania limfocytów krwi obwodowej w gradiencie fikolu i uropoliny
Prostojj metod izolirovanija limfocitov perifericheskojj krovi v gradiente fikola i uropolina
A simple method for isolation of peripheral blood lymphocytes in ficol-uropolin gradient
Autorzy:
Koch, I.
Kłos, M.
Powiązania:
https://bibliotekanauki.pl/articles/2188664.pdf
Data publikacji:
1978
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1978, 14, 1; 29-33
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of cellular isoform of prion protein on the surface of peripheral blood lymphocytes among women exposed to low doses of ionizing radiation
Autorzy:
Klucinski, P
Mazur, B.
Hrycek, A.
Masluch, E.
Cieslik, P.
Kaufman, J.
Martirosian, G.
Powiązania:
https://bibliotekanauki.pl/articles/50336.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
blood lymphocyte
ionizing radiation
low dose
lymphocyte
cellular isoform
woman
radiation exposure
expression
prion protein
Opis:
Ionizing radiation affects the expression of adhesive and co-stimulatory molecules in lymphocytes. The physiological function of cellular isoform of prion protein (PrPc) is little known. Evidences indicate a link between lymphocytes activation and PrPc expression on their surface; however, no direct effect of radiation on PrPc level in these cells was investigated. The objective of this study was to determinate the effect of low doses of ionizing radiation on the expression of PrPc on the surface peripheral blood lymphocytes in the women operating X-ray equipment. In 36 female workers and 30 persons of the control group the PrPc expression on CD3 (T lymphocytes), CD4 (T helper), CD8 (T cytotoxic) and CD19 (B lymphocytes), as well as the percentage of lymphocytes with PrPc on their surface, were tested. Subgroups with respect to age and length of employment were selected. A signifi cant increase was observed in PrPc expression on CD3 and CD4 with lowered PrPc level on CD8 and percentage of CD8 cells with PrPc in workers compared to control. The PrPc level did not show signifi cant changes in subgroups in relation to age (below and over 40 years old) both in the investigated and control groups, whereas a lower percentage of PrPc expressing CD19 cells showed in employed women below 40 years of age. A signifi cant decrease was found in PrPc expression on the surface of CD3, CD4 and CD8 cells in the subgroup employed for over 10 years than in the subgroup with less than 10 years of employment.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of treatment with methimazole on the Bcl-2 expression in CD8+ peripheral blood lymphocytes in children with Graves’ disease
Autorzy:
Klatka, M.
Grywalska, E.
Polak, A.
Rolinski, J.
Powiązania:
https://bibliotekanauki.pl/articles/50427.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ promieni rtg na zachowanie się błon lizosomalnych limfocytów, frakcji białek surowicy oraz IgG krwi obwodowej królika
Vlijanie rentgenovskogo izluchenija na povedenie lizosomnykh membran limfocitov, frakcijj belkov syvorotki i IgG perifericheskojj krovi krolika
Influence of roentgen rays on lysosomal membranes of lymphocytes, serum protein fractions and IgG in the peripheral blood of rabbits
Autorzy:
Grabarczyk, M.
Mdzewski, B.
Sokołowska, K.
Sitarska, E.
Powiązania:
https://bibliotekanauki.pl/articles/2189091.pdf
Data publikacji:
1976
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1976, 12, 6; 365-373
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
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