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    Wyświetlanie 1-12 z 12
    Tytuł:
    A review of paraoxonase 1 properties and diagnostic applications
    Autorzy:
    Kulka, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/32056.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Paraoxonase 1 (PON1) is an arylesterase associated with serum high density lipoprotein particles. Its name is derived from hydrolyzing one of several organophosphate compounds, namely paraoxon. Recent studies have shown that PON1 plays a protective role in diseases associated with oxidative stress such as atherosclerosis and diabetes mellitus. Studies have demonstrated reduction-oxidative state changes involving PON1 in humans and laboratory animal models. Although there is less information about the role of this enzyme in veterinary medicine, new data suggest that PON1 might be a new oxidative stress marker in animal patients, similarly to humans.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Species- and substrate-specific stimulation of human plasma paraoxonase 1 (PON1) activity by high chloride concentration.
    Autorzy:
    Bełtowski, Jerzy
    Wójcicka, Grażyna
    Marciniak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1043697.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    paraoxonase
    arylesterase
    lipid peroxidation
    oxidative stress
    Opis:
    Paraoxonase 1 (PON1), contained in plasma high-density lipoproteins, plays an important role in the protection of plasma lipoproteins and cell membranes from oxidative damage. Previous studies indicate that human PON1 is stimulated by high NaCl concentrations. The aim of this study was to characterize in more detail the effect of salts on serum PON1. Paraoxon-hydrolyzing activity of human serum was stimulated by 81.6% following the addition of 1 M NaCl. The effect of NaCl was dose-dependent between 0.5 and 2 M. PON1 activity toward phenyl acetate was reduced by 1 M NaCl by 55.2%. Both the paraoxon- and phenyl acetate-hydrolysing activity was slightly lower in heparinized plasma than in serum, but NaCl had similar stimulatory and inhibitory effects on these activities, respectively. In rat, rabbit, and mouse, NaCl reduced PON1 activity. KCl had a similar effect on human PON1 as NaCl. Sodium nitrite also stimulated human PON1 but much less effectively than chloride salts. In contrast, sucrose, sodium acetate and sodium lactate had no significant effect. NaBr was a less effective PON1 activator than NaCl, whereas the effect of NaJ was non-significant. The activity of human PON1 toward homogentisic acid lactone and γ-decanolactone was unaltered by NaCl. These data indicate that: 1) high concentrations of chlorides stimulate human PON1 activity toward paraoxon but not other substrates, 2) PON1 is inhibited by Cl- in other mammalian species, 3) the potency of human PON1 activation by halogene salts increases with decreasing atomic mass of the halide anion.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 4; 927-936
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    High density lipoprotein subfractions and paraoxonase 1 in children
    Autorzy:
    Muchová, Jana
    Andrezálová, Lucia
    Oravec, Stanislav
    Nagyová, Zuzana
    Garaiova, Iveta
    Ďuračková, Zdeňka
    Powiązania:
    https://bibliotekanauki.pl/articles/1038781.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    lipoproteins
    LDL-lipoproteins
    HDL-lipoproteins
    children
    paraoxonase 1
    Opis:
    The Lipoprint system (Quantimetrix Corp., CA, USA), enables the determination of 10 high density lipoprotein (HDL) subfractions in contrast to the 5 HDL subfractions that can be determined by ultracentrifuge analysis. HDL subfractions, and their relationships to the arylesterase (PON1-A) and lactonase (PON1-L) activities of paraoxonase 1 (PON1), together with total-, very low density lipoprotein- and low density lipoprotein (LDL)-cholesterol and LDL subfractions were investigated in the serum of 27 mildly hypercholesterolemic children and 21 healthy controls. Our results suggest the antiatherogenity of large HDL (L-HDL) subfractions and the atherogenity of small HDL (S-HDL) subfractions in the study groups. However, the relationship between the intermediate HDL (I-HDL) subfractions with the LDL subfractions and other lipoproteins did not suggest that I-HDL subfractions are antiatherogenic. No significant association between PON1-A and the HDL subfractions was found. In contrast, PON1-L activity positively correlated with the antiatherogenic large HDL1 subfraction and negatively with intermediate HDL subfractions 4, 5 and 6. Our results contribute to the knowledge of the roles of total HDL and ten individual HDL subfractions in children and adolescents.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 3; 555-563
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antyoksydacyjne właściwości i przeciwmiażdżycowe działanie paraoksonazy 1
    Antioxidant and antiatherosclerotic properties of paraoxonase 1
    Autorzy:
    Kupczyk, D.
    Karczmarska-Wódzka, A.
    Studzińska, R.
    Sikora, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/172751.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    paraoksonaza 1
    stres oksydacyjny
    miażdżyca
    paraoxonase 1
    oxidative stress
    atherosclerosis
    Opis:
    This overview will discuss the Paraoxonase 1 (PON1) in arteriosclerosis diseases. Atherosclerosis is one of lifestyle diseases and affects greater number of people. Ischemic heart disease, acute coronary syndromes or stroke are the clinical symptoms of atherosclerosis and are the most common cause of morbidity especially in middle and old age people. In atherosclerosis, in the space between the endothelium and the muscular layer in the wall of a blood vessel, accumulates deposits consisting of macrophages, lipoprotein, low density foam cells and extracellular concentrations of cholesterol. In this way fatty streaks are formed which are early stage atherosclerotic lesions. With the passage of time they are joined by elements of fibrous connective tissue that undergo hypertrophy. They begin to surround primarily created the fireplace of inflammation and separate them from the rest of the blood vessel [1]. Further research if needed to better understanding the mechanisms related to atherosclerosis development and plaque instability because it may have important clinical implications for the identification of high-risk patients. The present review tries to summarize the current knowledge on the role of PON1 in the formation of atherosclerotic plaque is the goal of current research [2].
    Źródło:
    Wiadomości Chemiczne; 2017, 71, 1-2; 3-36
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Activity of paraoxonase 1 (PON1) and its relationship to markers of lipoprotein oxidation in healthy Slovaks
    Autorzy:
    Sumegová, Katarína
    Blažíček, Pavel
    Waczulíková, Iveta
    Žitňanová, Ingrid
    Ďuračková, Zdeňka
    Powiązania:
    https://bibliotekanauki.pl/articles/1041175.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    paraoxonase 1
    atherosclerosis
    ox-LDL
    HDL
    Opis:
    Low-density lipoproteins (LDLs), when modified by free radicals derived from artery wall cells, induce atherosclerosis. In contrast to oxidized LDL (ox-LDL), high-density lipoproteins (HDLs) are able to prevent atherosclerosis through a protein with antioxidant properties, paraoxonase 1 (PON1). The purpose of this study was to explore the association between the activity of HDL-associated PON1 and circulating ox-LDL as well as to investigate the relationship between ox-LDL and parameters of lipid profile in thirty Slovaks aged 21-73 years because recent studies have presented controversial results concerning PON1 and its role in LDL oxidation. For determination of circulating ox-LDL sandwich ELISA was used and other lipid parameters were determined by routine laboratory analyses. PON1 activities were assayed by two synthetic substrates - paraoxon and phenyl acetate. Lipid peroxides were determined spectrophotometrically. Of the lipid parameters examined, ox-LDL level correlated positively with total (P < 0.0001) and LDL-cholesterol (P < 0.001). Triacylglycerols (TAG) (P < 0.001), lipid peroxides (P < 0.01) and atherogenic index (AI = total cholesterol/HDL) (P < 0.0001) were also strongly correlated with ox-LDL. No inverse relationships were observed between ox-LDL and HDL-cholesterol or arylesterase/paraoxonase activities of PON1. Furthermore, it was found that ox-LDL (P < 0.01) and lipid peroxides (P < 0.05) were significantly higher in men than in women. PON1 arylesterase activity was marginally affected by sex. The results of this study suggest that the anti-atherogenic properties of HDLs are not directly related to their total concentration and that PON1 activity determined towards synthetic compounds (paraoxon and phenyl acetate) reflects no association with markers of oxidative stress. Furthermore, it follows from our results that men are more susceptible to developing atherosclerosis compared to women.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 4; 783-787
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Paraoxonase-1 activities in children and adolescents with type 1 diabetes mellitus
    Autorzy:
    Craciun, Elena
    Leucuta, Daniel
    Rusu, Razvan
    David, Bianca
    Cret, Victoria
    Dronca, Eleonora
    Powiązania:
    https://bibliotekanauki.pl/articles/1038772.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    paraoxonase-1
    lactonase
    arylesterase
    children
    type 1 diabetes mellitus
    Opis:
    Background: Paraoxonase-1 is an HDL-associated esterase that acts as an anti-atherogenic agent by protecting LDL from oxidation. This study investigates paraoxonase-1 activities in children and adolescents with type 1 diabetes mellitus and possible associations with other biochemical markers. Patients and methods: The study enrolled 82 children and adolescents with type 1 diabetes mellitus and 41 controls with similar age and gender distribution. Serum paraoxonase-1 arylesterase and salt-stimulated paraoxonase activities were assessed by measuring the rates of phenyl acetate and paraoxon hydrolysis, respectively; paraoxonase-1 lactonase activity and oxidized LDL were assessed by a pH-sensitive colorimetric assay and ELISA, respectively. Glycated haemoglobin HbA1c and lipid profile were assayed with an immunoturbidimetric method and commercially available kits, respectively. Results: We found lower paraoxonase-1 activities in diabetics when compared to controls. The decrease was statistically significant only for the lactonase activity, the difference being higher when referring to the subgroup with poor glycaemic control. The lactonase activity/HDL ratio was also lower in diabetics vs. controls, but without statistical significance. Both lactonase and arylesterase activities were negatively correlated with HbA1c in diabetics, but only the latter was statistically significant (ρ = -0.21, P = 0.055; ρ = -0.24, P = 0.03, respectively). A correlation coefficient of ρ = 0.196 (P = 0.078) was found between oxidized LDL and HbA1c. Conclusion: All paraoxonase-1 activities were lower in diabetic children and adolescents, but only the decrease in the lactonase activity was statistically significant. Although lipid profile and glycaemic control were altered in diabetics, no differences were observed between groups regarding oxidized LDL level.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 3; 511-515
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Compensatory paraoxonase and arylesterase levels in hyperbaric oxygen treatment of sudden sensorineural hearing loss
    Autorzy:
    Eren, Esin
    Yilmaz, Necat
    Yildirim, Furkan
    Giray, Ozlem
    Powiązania:
    https://bibliotekanauki.pl/articles/1375132.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej
    Tematy:
    paraoxonase
    hyperbaric oxygen therapy
    inflammation
    oxidative stress
    reactive oxygen species arylesterase
    Opis:
    Objective: Paraoxonase1 (PON1) and Arylesterase (ARE) levels are associated with reduced risk of atherosclerosis. The functional status of high density lipoprotein (HDL) is closely related to the PON1/ARE enzyme activity. Functional changes in treatment of sudden sensorineural hearing loss (SSNHL) may be achieved by post-translational modification of lipid metabolism induced by hyperbaric oxygen therapy (HBOT). Methods: Men patients with SSNHL who met the research criteria were included in the study. HBOT was performed on average 30 sessions. Laboratory measurements were made at the beginning and end of HBOT for the same patients. Serum levels of PON1/ARE and routine lipid laboratory parameters were measured to determine possible changes in SSNHL after HBOT. Results: In this study, a reducing effect on PON1 enzyme amount of long-term HBOT was detected. The serum PON1 amount of patients with SSNHL was 19.7 ± 2.7 ng / mL (mean ±} SD) before HBOT, and the serum PON1 decreased to 17.0 ± 2.1 ng / mL (mean ±} SD) after 30 sessions of HBOT. This decrease in PON1 levels was statistically significant (p =0.035). There was also a statistically significant decrease in the enzyme activity of ARE in the SSNHL patients (p=0.024). Conclusion: This preliminary study showed a significant decrease in serum PON1/ARE enzyme content in SSNLH patients with HBOT. In fact, it can be assumed that HBOT has no adverse effect on HDL functionality. However, the decrease in PON1 level by HBOT with 30 or more sessions may be important for the antioxidant function of HDL.It may possibly cause post-translational changes in antioxidant defense mechanisms due to increased oxidative stress with HBOT. In conclusion, larger clinical studies are needed to determine the possible effects of HBOT on HDL-related PON1/ARE functionality in SSNHL.
    Źródło:
    Polish Hyperbaric Research; 2020, 1(70); 47-52
    1734-7009
    2084-0535
    Pojawia się w:
    Polish Hyperbaric Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of high sucrose diet, gemfibrozil, and their combination on plasma paraoxonase 1 activity and lipid levels in rats
    Autorzy:
    Macan, Marija
    Vrkić, Nada
    Vrdoljak, Ana
    Radić, Božica
    Bradamante, Vlasta
    Powiązania:
    https://bibliotekanauki.pl/articles/1040374.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    paraoxonase
    lipids
    high sucrose diet
    gemfibrozil
    Opis:
    We investigated the influence of high sucrose diet (HSD) after 3 or 5 weeks of administration on paraoxonase 1 (PON1) activity in plasma of normolipidemic rats and the relationship between serum PON1 activity, triacylglycerides (TGs), HDL and total cholesterol vs. the control group of rats fed normal, control diet (CD). Because the data about the influence of gemfibrozil (GEM) on PON1 activity are controversial, we also investigated its effects (administration in the 4th and 5th week in rats on HSD and CD) on plasma PON1 activity and lipid levels in normolipidemic rats, and in rats with hypertriglyceridemia caused by HSD. Our results obtained in rats on HSD show a significant increase of plasma TGs levels by 47% (P<0.05) after 5 weeks of treatment, and PON1 activity by 32% and 23% (P<0.05) after 3 and 5 weeks, but without change in lipid levels vs. rats on CD. In the rats on CD and HSD, GEM caused a significant decrease of PON1 activity by 44% and 33%, while a significant decrease of TGs level by 38% (P<0.05) was measured only in rats on CD. The effects of GEM on total cholesterol, HDL and LDL in both groups of rats were typical for its action on lipoprotein metabolism. Because GEM in the rat liver stimulates proliferation of peroxisomes, β oxidation, and production of H2O2, it is possible that the oxidative stress induced by GEM damages hepatocytes and lowers the synthesis of PON1.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 3; 321-326
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Paraoxonase 1 and dietary hyperhomocysteinemia modulate the expression of mouse proteins involved in liver homeostasis
    Autorzy:
    Suszyńska-Zajczyk, Joanna
    Jakubowski, Hieronim
    Powiązania:
    https://bibliotekanauki.pl/articles/1039222.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Pon1
    high-methionine diet
    hyperhomocysteinemia
    mouse liver proteome
    Opis:
    Homocysteine (Hcy), a product of methionine metabolism, is elevated by the consumption of a high-methionine diet that can cause fatty liver disease. Paraoxonase 1 (Pon1), a hydrolase expressed mainly in the liver and carried in the circulation on high-density lipoprotein, participates in Hcy metabolism. Low Pon1 activity is linked to fatty liver disease. We hypothesize that hyperhomocysteinemia and low Pon1 induce changes in gene expression that could impair liver homeostasis. To test this hypothesis, we analyzed the liver proteome of Pon1-/- and Pon1+/+ mice fed a high methionine diet (1% methionine in the drinking water) for 8 weeks using 2D IEF/SDS-PAGE gel electrophoresis and MALDI-TOF mass spectrometry. We identified seven liver proteins whose expression was significantly altered in Pon1-/- mice. In animals fed with a control diet, the expression of three liver proteins involved in lipoprotein metabolism (ApoE), iron metabolism (Ftl), and regulation of nitric oxide generation (Ddah1) was up-regulated by the Pon1-/- genotype. In mice fed with a high-methionine diet, expression of four liver proteins was up-regulated and of three proteins was down-regulated by the Pon1-/- genotype. The up-regulated proteins are involved in lipoprotein metabolism (ApoE), energy metabolism (Atp5h), oxidative stress response (Prdx2), and nitric oxide regulation (Ddah1). The down-regulated proteins are involved in energy metabolism (Gamt), iron metabolism (Ftl), and catechol metabolism (Comt). Expression of one protein (Ftl) was up-regulated both by the Pon1-/- genotype and a high-methionine diet. Our findings suggest that Pon1 interacts with diverse cellular processes - from lipoprotein metabolism, nitric oxide regulation, and energy metabolism to iron transport and antioxidant defenses - that are essential for normal liver homeostasis and modulation of these interactions by a high-methionine diet may contribute to fatty liver disease.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 815-823
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serum paraoxonase 1 (PON1) activity and lipid metabolism parameters changes in different production cycle periods of Holstein - Friesian, Polish Red and Norwegian breeds
    Autorzy:
    Kulka, M.
    Kolodziejska-Lesisz, J.
    Klucinski, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30093.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    We investigated the measurement of paraoxonase 1 (PON1), as a potential marker of redox state changes in dairy cows, its involvement in lipid metabolism and compared it with superoxide dismutase (SOD) activity changes. We also evaluated lipid metabolism parameters associated with dairy production. PON1 paraoxonase and arylesterase acitvities, SOD activity, beta-hydroxybutyrate (BHB), uric acid (UA), high density lipoprotein (HDL), low density lipoprotein (LDL), cholesterol and triglyceride concentrations were measured in Holstein-Friesian, Polish Red and Norwegian breeds serum in two production cycles. Our data showed a significant postpartum depletion in PON1 activity and lipoprotein and lipid products concentrations, with elevated BHB values. However, there were no significant changes in SOD activity and uric acid concentrations in Holstein-Friesian and Polish Red breeds after calving. At lactation peak there was a significant SOD activity decrease correlated with standardized PON1 activity depletion in all examined breeds. The results suggest that PON1 might be a better parameter for minimal redox state changes in serum, shortly after labour in the examined breeds.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serum paraoxonase 1 activity and lipid metabolism parameter changes in Dachshunds with chronic mitral valve disease.Assessment of its diagnostic usefulness
    Autorzy:
    Kulka, M.
    Garncarz, M.
    Parzeniecka-Jaworska, M.
    Kluciński, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087879.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    PON1
    chronic mitral valve disease
    Dachshunds
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 723-729
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Inference of oxidative stress in patients with hypothyroidism
    Autorzy:
    Rada, Feryal
    Powiązania:
    https://bibliotekanauki.pl/articles/40555549.pdf
    Data publikacji:
    2024-06-30
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    glutathione
    hypothyroidism
    malondialdehyde
    oxidative stress
    paraoxonase-1
    Opis:
    Introduction and aim. Oxidative stress is one of the complications that accompany defects in thyroid hormone levels. This study was designed to evaluate oxidative stress markers in patients with hypothyroidism. Material and methods. This case control study was comprised of forty-two hypothyroid patients aged 36–46 years and forty age matched (35–43 years) healthy control participants randomly selected from the Endocrine Clinic of Al-Yarmook Hospital in Iraq. Blood levels of thyroid hormones malondialdehyde, glutathione, and paraoxonase-1 were assessed. Body mass index was calculated for each patient and control participant. Results. Regarding the data of oxidative stress markers in hypothyroid patients compared to controls, a significant elevation was reported in blood levels of malondialdehyde and a significant reduction was found in blood levels of glutathione (p=0.031). On the other hand, the blood levels of paraoxonase-1 were significantly different in hypothyroid patients compared with the control. Conclusion. Elevated blood levels of malondialdehyde and declined blood levels of glutathione in hypothyroid patients are a signal of oxidative stress and consequently increase the risk of cardiovascular complications.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2024, 22, 2; 270-274
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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