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Wyszukujesz frazę "osteogenic differentiation" wg kryterium: Wszystkie pola

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    Wyświetlanie 1-12 z 12
    Tytuł:
    Titanium alloy coated with phloroglucinol-enriched collagen fibrils regulates osteogenic differentiation and inflammation
    Autorzy:
    Mieszkowska, Anna
    Beaumont, Harrison
    Martocq, Laurine
    Koptyug, Andrey
    Surmeneva, Maria A.
    Surmenev, Roman A.
    Gurzawska, Katarzyna A.
    Naderi, Javad
    Douglas, Timothy E.L
    Powiązania:
    https://bibliotekanauki.pl/articles/1844893.pdf
    Data publikacji:
    2020
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    titanium alloys
    collagen
    cells
    stopy tytanu
    kolagen
    komórki
    Źródło:
    Engineering of Biomaterials; 2020, 23, 158 spec. iss.; 18
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Osteogenic differentiation of human mesenchymal stem cells from adipose tissue and Whartons jelly of the umbilical cord
    Autorzy:
    Zajdel, Alicja
    Kałucka, Magdalena
    Kokoszka-Mikołaj, Edyta
    Wilczok, Adam
    Powiązania:
    https://bibliotekanauki.pl/articles/1038664.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    adipose derived mesenchymal stem cells
    Wharton's jelly derived mesenchymal stem cells
    osteogenic differentiation
    Opis:
    Induced osteogenesis of mesenchymal stem cells (MSCs) may provide an important tool for bone injuries treatment. Human umbilical cord and adipose tissue are routinely discarded as clinical waste and may be used as noncontroversial MSCs sources. It still remains to be verified which source of MSCs is the most suitable for bone regeneration. The aim of this research was to investigate the osteogenic potential of human MSCs derived from adipose tissue (AT-MSCs) and Wharton's jelly of the human umbilical cord (WJ-MSCs) differentiated under the same conditions. Osteogenic differentiation of MSCs was detected and quantified by alizarin red S (ARS) staining for calcium deposition and alkaline phosphatase (ALP) activity, osteoprotegerin (OPG), and osteocalcin (OC) secretion measurements. Under osteogenic conditions, after 21 days of differentiation, the measured ALP activity and calcium deposition were significantly higher in the AT-MSCs than in the WJ-MSCs, while the OPG and OC secretion were higher in the WJ-MSCs vs. AT-MSCs. Low concentrations of OPG and high levels of OC in AT-MSCs and WJ-MSCs, prove that these cells reached an advanced stage of the osteogenic differentiation. The levels of OC secreted by AT-MSCs were lower than by WJ-MSCs. Both cell types, AT-MSCs and WJ-MSCs possess a potential to differentiate towards the osteogenic lineage. The observed differences in the levels of osteogenic markers suggest that after 21-days of osteogenic differentiation, the AT-MSCs might have reached a more advanced stage of differentiation than WJ-MSCs.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 2; 365-369
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of nano-HA-based biomaterial on macrophage polarization and osteogenic differentiation in co-culture system in vitro
    Autorzy:
    Kazimierczak, Paulina
    Przekora, Agata
    Powiązania:
    https://bibliotekanauki.pl/articles/1844828.pdf
    Data publikacji:
    2021
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Źródło:
    Engineering of Biomaterials; 2021, 24, 163; 14
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Growth and osteogenic differentiation of human osteoblast-like cells on nanofibrous scaffolds loaded with diamond nanoparticles: improvement or impairment?
    Autorzy:
    Musilkova, J.
    Stankova, L.
    Potocky, S.
    Kromka, A.
    Stranska, D.
    Bacakova, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/285888.pdf
    Data publikacji:
    2016
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    nanofibrous scaffold
    diamond nanoparticles
    human osteoblasts
    Źródło:
    Engineering of Biomaterials; 2016, 19, 138; 32
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Osteoblasts response to novel chitosan/agarose/hydroxyapatite bone scaffold – studies on MC3T3-E1 and hFOB 1.19 cellular models
    Autorzy:
    Kazimierczak, Paulina
    Vivcharenko, Vladyslav
    Truszkiewicz, Wiesław
    Wójcik, Michał
    Przekora, Agata
    Powiązania:
    https://bibliotekanauki.pl/articles/284277.pdf
    Data publikacji:
    2019
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    bone tissue engineering
    biocompatibility
    osteoconductivity
    cell growth
    osteogenic differentiation
    Opis:
    Since it is known that various cell lines may ex-press different behaviours on the scaffolds surface, a comprehensive analysis using various cellular mo-dels is needed to evaluate the biomedical potential of developed biomaterials under in vitro conditions. Thus, the aim of this work was to fabricate bone scaffolds composed of a chitosan-agarose matrix reinforced with nanohydroxyapatite and compare the biological response of two cell lines, i.e. mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4) and human foetal osteoblasts (hFOB 1.19). Within this study, the osteoblasts number on the scaffold surface and the osteogenic markers level produced by MC3T3-E1 and hFOB 1.19 cells were determined. Furthermore, changes in calcium and phosphorous ions concentrations in the culture media dedicated for MC3T3-E1 and hFOB 1.19 were estimated after the biomaterial incubation. The obtained results proved that the fabricated biomaterial is characterized by biocompatibility and osteoconductivity since it favours osteoblasts attachment and growth. It also supports the production of osteogenic markers (collagen, bALP, osteocalcin) by MC3T3-E1 and hFOB 1.19 cells. Interestingly, the developed biomaterial exhibits different ion reactivity values in the two culture media dedicated for the mentioned cell lines. It was also revealed that mouse and human osteoblasts differ in the cellular response to the fabricated scaffold. Thus, the use of at least two various cellular models is recommended to carry out a reliable biological characterization of the novel biomaterial. These results demonstrate that the tested bone scaffold is a promising biomaterial for bone regeneration applications, however further biological and physicochemical experiments are essential to fully assess its biomedical potential.
    Źródło:
    Engineering of Biomaterials; 2019, 22, 151; 24-29
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged ferroelectric crystal surfaces
    Autorzy:
    Bacakova, L.
    Vandrovcova, M.
    Vanek, P.
    Petzelt, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/284786.pdf
    Data publikacji:
    2016
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biomaterials
    mesenchymal stem cells
    crystal surfaces
    Źródło:
    Engineering of Biomaterials; 2016, 19, 138; 28
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of substrate stiffness on differentiation of umbilical cord stem cells
    Autorzy:
    Witkowska-Zimny, Małgorzata
    Walenko, Katarzyna
    Wałkiewicz, Anna
    Pojda, Zygmunt
    Przybylski, Jacek
    Lewandowska-Szumieł, Małgorzata
    Powiązania:
    https://bibliotekanauki.pl/articles/1039745.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    substrate stiffness
    osteogenic differentiation
    umbilical cord mesenchymal stromal/stem cells
    Opis:
    Tissue formation and maintenance is regulated by various factors, including biological, physiological and physical signals transmitted between cells as well as originating from cell-substrate interactions. In our study, the osteogenic potential of mesenchymal stromal/stem cells isolated from umbilical cord Wharton's jelly (UC-MSCs) was investigated in relation to the substrate rigidity on polyacrylamide hydrogel (PAAM). Osteogenic differentiation of UC-MSCs was enhanced on stiff substrate compared to soft substrates, illustrating that the mechanical environment can play a role in differentiation of this type of cells. These results show that substrate stiffness can regulate UC-MSCs differentiation, and hence may have significant implications for design of biomaterials with appropriate mechanical properties for regenerative medicine.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 2; 261-264
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Integrin αv signaling influences phenotype and maturation of primary human osteoblasts on alumina surface
    Autorzy:
    Wróbel, E.
    Witkowska-Zimny, M.
    Mrówka, P.
    Głodkowska-Mrówka, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/284664.pdf
    Data publikacji:
    2014
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    integrin
    human bone-derived cells
    osteogenic differentiation
    cell adhesion
    biomaterial
    Opis:
    Due to the growing interest in stem cells application in tissue engineering the better understanding of primary human osteoblasts behavior in vitro, on biomaterial surface, is required. Among other molecules integrins may be taken into account as being involved in these phenomena. Integrins are a family of cell adhesion receptors, which may regulate many cellular functions e.g., adhesion, motility, phenotype and cell maturation. The aim of this study was to determine the effect of the biomaterial surfaces and αv integrin signaling pathway on the behavior, phenotype and maturation of human osteoblasts in vitro. Human bone derived cells (HBDCs) obtained from adult femoral bone fragments were cultured on both alumina disks and tissue culture polystyrene (TCPS) dishes. After 7, 14, and 21 days of culture, localization and mRNA expression level of αv integrin subunits and BGLAP (osteocalcin) on polystyrene were analyzed in addition, we treated the cell cultures with monoclonal antibodies against human αv integrin to block its ligand-binding activity, on both alumina and TCPS substrates. We found that the αv integrin was present in focal contacts and cell cytoplasm at subsequent stages of cell maturation and the level of αv integrin mRNA was the highest in mature osteoblasts. Blocking αv integrin transduction pathway caused changes in cell activity and morphology, decreased cells proliferation on TCPS and reduced expression of alkaline phosphatase (ALP) on both materials. The results suggest that αv integrin is involved as an important receptor facilitating osteogenic differentiation.
    Źródło:
    Engineering of Biomaterials; 2014, 17, 127; 33-39
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged and uncharged ferroelectric crystal surfaces
    Autorzy:
    Vandrovcova, M.
    Bacakova, L.
    Vanek, P.
    Petzelt, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/285786.pdf
    Data publikacji:
    2016
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    electroactive ceramics
    surface charge
    cell number
    resazurin
    type I collagen
    alkaline phosphatase
    osteocalcin
    bone matrix mineralization
    Opis:
    The cell-material interaction is significantly influenced by the physicochemical properties of the material surface, including its electrical charge. In this study, the effect of the surface polarity of ferroelectric LiNbO3 single crystals on the adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells was investigated. The cells were cultured on the normal-to-plane poled and in-plane poled plates resulting in positive, negative and zero surface charge. The number of initially adhering cells on day 1 after seeding, their spreading, shape, and their metabolic activity, production of type I collagen, activity of alkaline phosphatase and mineralization in the following days of cultivation (days 6 and 20) were comparable on all three tested surfaces. However, significant differences were found in the expression of mRNA for type I collagen, alkaline phosphatase and osteocalcin, i.e. an early, medium-term and late arkers of osteogenic cell differentiation, respectively. On day 20, the expression of type I collagen was significantly lower in cells on negatively-charged than on non-charged surfaces. Moreover, the expression of alkaline phosphatase and osteocalcin was higher in cells on positively-charged than on negatively-charged surfaces. These differences were generally more pronounced in standard cell culture medium than in osteogenic medium, which could, at least partly, mask the influence of the material surface properties on the cell behaviour. Thus, positively-charged LiNbO3 surfaces seemed to be more suitable for the osteogenic differentiation of bone marrow mesenchymal stem cells than the negatively-charged surfaces.
    Źródło:
    Engineering of Biomaterials; 2016, 19, 135; 2-7
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    EF1α is a suitable housekeeping gene for RT-qPCR analysis during osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells
    Autorzy:
    Chen, Xingyun
    Zhang, Bo
    Zhao, Yan
    Liu, Ping
    Zhou, Yuanguo
    Powiązania:
    https://bibliotekanauki.pl/articles/1039535.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    EF1α; RGS4; 18S rRNA; RT-qPCR; RPL 13a; CCG-1986
    Opis:
    The expression of predominant housekeeping genes used in RT-qPCR can vary during development and differentiation. The frequently used housekeeping genes (ACTB, GAPDH, 18S rRNA, EF1α and RPL 13a) were evaluated during an early stage of the osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells (mMSCs) (under normal conditions or treated with CCG-4986) to identify housekeeping genes whose expression remained constant during osteogenic differentiation. When we used RGS4 mRNA, which was determined as copy number per μg of total RNA, to normalize gene expression, we observed that the relative EF1α expression profile was consistent with RGS4 expression after treatment with CCG-4986. All the relative expression profiles of the EF1α, 18S rRNA, and RPL13a housekeeping genes were consistent with RGS4 profiles determined by measuring mRNA copies under normal osteogenic differentiation conditions. The expression profiles calibrated by ACTB and GAPDH were not consistent with those determined using mRNA copy number in untreated cells or cells treated with CCG-4986 under osteogenic differentiation conditions. Under normal osteogenic differentiation conditions, EF1α, 18S rRNA, and RPL 13a are suitable housekeeping genes for RT-qPCR analysis. However, EF1α is the only suitable gene upon CCG-4986 treatment.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 3; 381-386
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Telomerase enhances osteogenic ifferentiation of sheep bone marrow mesenchymal stem cells (BMSCs) by up-regulating PI3K/Akt pathway in vitro
    Autorzy:
    Zhu, X.
    Zhou, L.
    Liu, Z.
    Chen, X.
    Wei, L.
    Zhang, Z.
    Liu, Y.
    Zhu, Y.
    Wang, Y.
    Yang, X.
    Han, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087304.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bone marrow mesenchymal stem cells
    telomerase
    proliferation
    osteogenic differentiation
    PI3K/Akt signalling pathway
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 359-372
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ poli(ε-kaprolaktonu) na ekspresję i aktywność fosfatazy alkalicznej w ludzkich komórkach osteogennych
    The effect of poly(ε-caprolactone) on the expression and activity of alkaline phosphatase in human osteogenic cells
    Autorzy:
    Leszczyńska, J.
    Wójtowicz, J.
    Olkowski, R.
    Komasa, J.
    Ulański, P.
    Lewandowska-Szumieł, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/286189.pdf
    Data publikacji:
    2011
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    poli(ε-kaprolakton)
    osteoblasty
    fosfataza zasadowa
    różnicowanie
    poly(ε-caprolactone)
    osteoblasts
    alkaline phosphatase
    differentiation
    Opis:
    Poli(ε-kaprolakton) jest materiałem wykorzystywanym jako rusztowanie dla komórek w inżynierii tkankowej kości. Na podstawie danych z literatury oraz naszych własnych badań nad reakcją komórek osteogennych na bezpośredni kontakt z poli(ε-kaprolaktonem) można przypuszczać, iż materiał ten może wpływać na poziom markerów różnicowania komórek w kierunku osteoblastów. Celem niniejszej pracy było zbadanie wpływu poli(ε-kaprolaktonu) na ekspresję oraz aktywność wczesnego markera procesu różnicowania komórek osteogennych, jakim jest fosfataza zasadowa. Przy użyciu reakcji łańcuchowej polimerazy DNA z analizą ilości produktu w czasie rzeczywistym (real-time PCR) analizowano ekspresję genu fosfatazy zasadowej natomiast aktywność enzymu oznaczono kolorymetrycznym testem firmy Sigma. Otrzymane wyniki wskazują, iż kontakt ludzkich osteoblastów z powierzchnią poli(ε-kaprolaktonu) powoduje podwyższoną ekspresję genu fosfatazy zasadowej oraz podwyższoną aktywność tego enzymu. Fosfataza zasadowa nie jest specyficznym markerem osteoblastów, jednakże jej podwyższony poziom towarzyszy wczesnym etapom różnicowania w kierunku fenotypu komórek osteogennych. Uzyskane wyniki uzasadniają podjęcie dalszych badań nad możliwym wpływem poli(ε-kaprolaktonu) na różnicowanie osteoblastów.
    Poly(ε-caprolactone) is a material used as a scaffold for cells in bone tissue engineering. On the basis of data from literature as well as own research it was concluded that this material can influence the levels of markers of cell differentiation towards osteoblasts. The aim of this paper was to investigate the effect of poly(ε-caprolactone) on the expression and the activity of the early marker of the cell osteogenic differentiation process – alkaline phosphatase (ALP). Using the quantitative real time polymerase chain reaction (real-time PCR) gene expression of the alkaline phosphatase was analyzed; however, the activity of the enzyme was determined with colorimetric assay from the Sigma company. The obtained results indicated that the contact of human osteoblasts with the surface of poly(ε-caprolactone) causes an increased gene expression of alkaline phosphatase and an increased activity of this enzyme. Although a high level of ALP does not prove the PCL influence on the osteogenic differentiation of cells into mature osteoblasts, because this enzyme is a non-specific marker of the differentiation process. The obtained results justify undertaking further studies on the possible impact of poly(ε-caprolactone) on osteoblast differentiation.
    Źródło:
    Engineering of Biomaterials; 2011, 14, 103; 13-16
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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