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Wyświetlanie 1-15 z 33
Tytuł:
Hyperglycemia induced C2C12 myoblast cell cycle arrest and skeletal muscle atrophy by modulating sirtuins gene expression in rats
Autorzy:
Surinlert, P.
Thitiphatphuvanon, T.
Khimmaktong, W.
Pholpramool, C.
Tipbunjong, C.
Powiązania:
https://bibliotekanauki.pl/articles/2087134.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
diabetic mellitus
hyperglycemia
muscle atrophy
sirtuins
skeletal muscle
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 563-572
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania cytoszkieletu, skurczu mięśni i ruchu komórek w instytucie nenckiego
Studies on the cytoskeleton, muscle contraction and cell motility at the Nencki Institutue: historical view
Autorzy:
Rędowicz, Maria
Kuźnicki, Leszek
Powiązania:
https://bibliotekanauki.pl/articles/1033929.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Opis:
W 2018 roku Instytut Biologii Doświadczalnej im. M. Nenckiego w Warszawie obchodzi 100. rocznicę istnienia, a artykuł ten jest częścią specjalnego numeru poświęconego wiekowi badań przeprowadzonych w Instytucie w zakresie cytoszkieletu, ruchliwości komórek i skurczu mięśni. Artykuł opisuje historię badań oraz grupy zajmujące się tymi zagadnieniami przez dziesięciolecia. Ponadto, prezentuje wieloletni wkład naukowców z Instytutu Nenckiego w środowisko naukowe w Polsce i za granicą.
In 2018 the Nencki Institute of Experimental Biology in Warsaw, Poland, celebrates the 100th Aniversary of its setting-up and this article is a part of the special issue devoted to the century of a research conducted in the Institute related to the cytoskeleton, cell motility and muscle contraction. The article describes the history of the research, and groups working on the motile and contractile processes through the decades. Also, it presents a long-standing contribution of the Nencki Institute researchers to the scientific community in Poland and abroad.
Źródło:
Kosmos; 2018, 67, 1; 17-25
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of low - energy laser irradiation and antioxidant supplementation on cell apoptosis during skeletal muscle post - injury regeneration in pigs
Autorzy:
Otrocka-Domagala, I.
Mikolajczyk, A.
Pazdzior-Czapula, K.
Gesek, M.
Rotkiewicz, T.
Mikiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/30603.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to evaluate the effect of low-energy laser irradiation, coenzyme Q10 and vitamin E supplementation on the apoptosis of macrophages and muscle precursor cells during skeletal muscle regeneration after bupivacaine-induced injury. The experiment was conducted on 75 gilts, divided into 5 experimental groups: I – control, II – low-energy laser irradiation, III – coenzyme Q10, IV – coenzyme Q10 and vitamin E, V – vitamin E. Muscle necrosis was induced by injection of 0.5% bupivacaine hydrochloride. The animals were euthanized on subsequent days after injury. Samples were formalin fixed and processed routinely for histopathology. Apoptosis was detected using the TUNEL method. The obtained results indicate that low-energy laser irradiation has a beneficial effect on macrophages and muscle precursor cell activity during muscle post-injury regeneration and protects these cells against apoptosis. Vitamin E has a slightly lower protective effect, limited mainly to the macrophages. Coenzyme Q10 co-supplemented with vitamin E increases the activity of macrophages and muscle precursor cells, myotube and young muscle formation. Importantly, muscle precursor cells seem to be more sensitive to apoptosis than macrophages in the environment of regenerating damaged muscle.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Laser interference patterning of diamond-like carbon layers for directed migration and growth of smooth muscle cell depositions
Autorzy:
Marczak, J.
Kusinski, J.
Major, R.
Rycyk, A.
Sarzynski, A
Strzelec, M.
Czyz, K
Powiązania:
https://bibliotekanauki.pl/articles/174984.pdf
Data publikacji:
2014
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
laser interference lithography
Nd
YAG laser
diamond-like carbon (DLC) structuring
biocompatible scaffolds
Opis:
Seeding of cells on functional, biocompatible scaffolds is a crucial step in achieving the desired engineered tissue. The authors show the constructional solutions of the Nd:YAG pulse laser system with the Q-switch modulation for direct and interferential shaping of the surface of biocompatible materials. The two-channel interference system is distinguished by high control, simplicity and repetitiveness regarding laser energy level and dimensions of the surface structures. The experiments were conducted on hard, biocompatible substrates of amorphous carbon (diamond-like carbon, DLC) and were preliminarily tested on smooth muscle cell depositions.
Źródło:
Optica Applicata; 2014, 44, 4; 575-586
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxoplasma gondii and the host cells
Autorzy:
Dlugonska, H.
Powiązania:
https://bibliotekanauki.pl/articles/6614.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Toxoplasma gondii
host cell
host barrier
dendritic cell
muscle cell
Protozoa
parasite
mammal
bird
prevalence
Opis:
The protozoan Toxoplasma gondii, described by Nicolle and Manceaux in 1908, is a ubiquitous and cosmopolitan parasite that infects a wide range of mammal and bird species with high prevalence. The biological success of T. gondii is associated with the formation of a specific relationship between the parasite and host cells leading to the establishment of a latent, chronic infection. During primary infection, acquired mostly by the oral route, the quickly multiplying tachyzoites disseminate through the body crossing several structural-functional barriers as bloodbrain or blood-retina, then they transform into dormant bradyzoites which, enclosed in tissue cysts, occupy preferentially the brain, skeletal muscle and eye. Although T. gondii is able to infect all kinds of nucleated cells, it uses strictly defined host cells, dependent on the life-cycle phase and infection stage. The article discusses selected aspects of the parasite passing via the host body barriers as well as particular role of dendritic cells and skeletal muscle cells, used by the parasite as an very effective vehicle to disseminate throughout the host body or the site of long-term T. gondii persistence, respectively.
Źródło:
Annals of Parasitology; 2014, 60, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deformable cell and deformable tissue as an area for application of nanoscale mechanics
Autorzy:
Kaczmarek, J.
Powiązania:
https://bibliotekanauki.pl/articles/175397.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
artificial muscle
deformable cell
nanoscale mechanics
Opis:
Materials undergoing deformation based on various mechanisms are frequently applied to manufacturing of artificial muscle actuators. In this paper we introduce a methodological step towards more precise determination of investigation object related to such actuators. One defines a deformable cell and a deformable tissue in order to indicate which functions of such objects should be integrated for further applications. One accentuates role of nanoscale models of mechanics of materials as important on way towards design of the deformable cells.
Źródło:
Transactions of the Institute of Fluid-Flow Machinery; 2011, 123; 99-112
0079-3205
Pojawia się w:
Transactions of the Institute of Fluid-Flow Machinery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selected aspects of endothelial dysfunction and their influence on the atherosclerosis process modeled and analyzed by Petri net based approach
Autorzy:
Formanowicz, D.
Kozak, A.
Formanowicz, P.
Powiązania:
https://bibliotekanauki.pl/articles/80683.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
endothelial dysfunction
biological system
modelling
atherosclerosis
Petri net based model
immunological response
vasodilation
smooth muscle cell
oxidative stress
hypercholesterolemia
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transcriptional pattern of TGF-beta1 inhibitory effect on mouse C2C12 myoblasts differentiation
Autorzy:
Wicik, Z.
Sadkowski, T.
Jank, M.
Motyl, T.
Powiązania:
https://bibliotekanauki.pl/articles/30377.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
transforming growth factor-beta 1
myogenesis
microarray
DNA microarray
differentiation
myoblast
mouse
mice
muscle cell
myosin heavy chain
Opis:
The aim of the present study was to define the effect of TGF-β1 on C2C12 myoblasts myogenesis. TGF-β1 together with its receptor is a negative auto-paracrine regulator of myogenesis, which influences the proliferation, differentiation, and functions of muscle cells. TGF-β1 exerts highly significant inhibitory effect on differentiation of C2C12 mouse myoblasts manifested by the impairment of cell fusion and very low expression of myosin heavy chain. The study of differentiating C2C12 mouse myoblasts treated with TGF-β1 revealed 502 genes (436 down-regulated and 66 up-regulated) with statistically different expression. TGF-β1-regulated genes were identified to be involved in 29 biological processes, 29 molecular functions groups and 59 pathways. The strongest inhibiting effect of TGF-β1 was observed in the cadherin and Wnt pathways. The key-genes that could play the role of TGF-β1 targets during myoblasts differentiation was identified such as: Max, Creb1, Ccna2, Bax, MdfI, Tef, Tubg1, Cxcl5, Rho, Calca and Lgals4.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of satellite cells in skeletal muscle regeneration
Rola komórek satelitarnych w regeneracji mięśnia szkieletowego
Autorzy:
Harasiuk, Dorota
Górski, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1942938.pdf
Data publikacji:
2009
Wydawca:
Akademia Wychowania Fizycznego im. Bronisława Czecha w Krakowie
Tematy:
satellite cells
Myoblasts
Myogenic Regulatory Factors
self-renewal
Cell Transplantation
komórki satelitarne
mioblasty
mięśniowe czynniki transkrypcyjne
samoodnowa
przeszczepy komórkowe
Opis:
Skeletal muscles are composed of multinucleated fibers that cannot divide. They retain the ability to regenerate due to the presence of mononucleated cells, called satellite cells. Mitotically quiescent satellite cells are located between the sarcolemma and the basal lamina of the muscle fiber. They can be activated in response to muscle injury. Then they proliferate, differentiate and eventually fuse to damaged myofibers or fuse together to form new myofibers. A part of the activated cells escape differentiation and restore the pool of quiescent satellite cell under the basal lamina of the newly formed muscle fibers. A decline in the number and activity of satellite cells results in an impaired regeneration potential of aged muscle. However regeneration can be improved by modification of the microenvironment of the skeletal muscle. Satellite cells have been considered as a source for cell-based therapies in the treatment of diseases such as muscular dystrophies, heart failure, insufficient function of external urethral sphincter. They can be derived easily from skeletal muscle biopsies and cultured in vitro before cell transplantation. After injection to the host muscle they undergo a myogenic differentiation program. In the case of muscle dystrophy, clinical trials have demonstrated a lack of health improvement after transplantation. This was the result of the poor survival and limited migratory capacity of the injected cells. More promising results were obtained when satellite cells were transferred to the locally damaged muscles. This article demonstrates the role of satellite cells in skeletal muscle regeneration, and the possibilities of their use in cell-based therapies in the case of diseases where muscle fibers are impaired.
Źródło:
Medical Rehabilitation; 2009, 13(3); 25-32
1427-9622
1896-3250
Pojawia się w:
Medical Rehabilitation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization
Autorzy:
Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:
https://bibliotekanauki.pl/articles/285087.pdf
Data publikacji:
2009
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
Ar plasma discharge
biomaterials
low-density polyethylene
cell adhesion
cell proliferation
grafting
tissue engineering
vascular smooth muscle cells
Opis:
Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
Źródło:
Engineering of Biomaterials; 2009, 12, no. 89-91; 25-28
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aldolase A is present in smooth muscle cell nuclei
Autorzy:
Mamczur, Piotr
Dzugaj, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1040689.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aldolase
nucleus
heterochromatin
smooth muscle
isoenzymes
Opis:
Previously we have shown that aldolase (ALD; EC 4.1.2.13) is present in cardiomyocyte nuclei. Now, we focused our attention on ALD localization in smooth muscle cells. Immunocytochemical methods were used to study the subcellular localization of ALD. Aldolase was localized in the cytoplasm as well as in the nuclei. Within the nuclei ALD was located in the heterochromatin region. Native polyacrylamide gel electrophoresis followed by aldolase activity staining in gel was used to study the ALD isoenzyme pattern in porcine smooth muscle cells. Two ALD isoenzymes, A and C, were found in these cells but in the nuclei only the muscle isoenzyme was detected. To support the nuclear localization of ALD, measurement of aldolase activity in the smooth muscle cell nuclei isolated from porcine stomach was performed. The ALD activity in the isolated nuclei was detectable only after preincubation of the nuclear fraction with Triton X-100 and high concentration of KCl.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 799-805
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
Autorzy:
Parizek, M.
Base, T.
Londesborough, M. G. S.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/285409.pdf
Data publikacji:
2008
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
metal coating
gold film
carboranethiol
cell adhesion
cell spreading
cell proliferation
biomaterials
tissue engineering
surgical implants
Opis:
Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2B10H11 (A), 1,2-(HS)2-1,2-C2B10H10 (B), 9,12-(HS)2-1,2-C2B10H10 (C) and 1,12-(HS)2-1,12- C2B10H10 (D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
Źródło:
Engineering of Biomaterials; 2008, 11, no. 81-84; 117-119
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
Autorzy:
Parizek, M.
Base, T.
Londesborough, M. G. S.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/286249.pdf
Data publikacji:
2008
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
implanty chirurgiczne
biomateriały
powłoki metalowe
inżynieria materiałowa
metal coatings
gold film
carboranethiol
cell adhesion
cell spreading
cell proliferation
biomaterials
tissue engineering
surgical implants
Opis:
Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2 B10H11(A), 1,2-(HS)2-1,2-C2B10H10(B), 9,12-(HS)2-1,2-C2B10H10(C) and 1,12-(HS)2-1,12- C2B10H10(D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
Źródło:
Engineering of Biomaterials; 2008, 11, 75; 6-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge
Autorzy:
Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:
https://bibliotekanauki.pl/articles/284295.pdf
Data publikacji:
2007
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomateriały
inżynieria tkankowa
Ar plasma discharge
high density and low density polyethylene
cell adhesion
cell proliferation
vascular smooth muscle cells
biomaterials
tissue engineering
Opis:
The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.
Źródło:
Engineering of Biomaterials; 2007, 10, no. 67-68; 1-4
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physiology and pathophysiology of vascular signaling controlled by guanosine 3',5'-cyclic monophosphate-dependent protein kinase.
Autorzy:
Birschmann, Ingvild
Walter, Ulrich
Powiązania:
https://bibliotekanauki.pl/articles/1043273.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
insulin
protein kinase
NO
smooth muscle cell
cGMP
cardiovascular diseases
Opis:
Recent medical advances suggest that the cellular natriuretic peptide/cGMP and NO/cGMP effector systems represent important signal transduction pathways especially in the cardiovascular system. These pathways also appear to be very interesting targets for the possible prevention of cardiovascular diseases. Exciting candidates for prevention include cGMP-dependent signaling networks initiated by natriuretic peptides (NP) and nitric oxide (NO) which are currently explored for their diagnostic and therapeutic potential. cGMP signaling contributes to the function and interaction of several vascular cell types, and its dysfunction is involved in the progression of major cardiovascular diseases such as atherosclerosis, hypertension and diabetic complications. This review will take a focussed look at key elements of the cGMP signaling cascade in vascular tissue. Recent advances in our knowledge of cGMP-dependent protein kinases (cGK, also known as PKG), the potential for assessing the functional status of cGMP signaling and the possible cross talk with insulin signaling will be reviewed.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 397-404
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 33

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