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Tytuł:
Conformational studies of tachykinin peptides using NMR spectroscopy
Autorzy:
Rodziewicz, S.
Xiao-Fei, Qi.
Rolka, K.
Powiązania:
https://bibliotekanauki.pl/articles/1954059.pdf
Data publikacji:
1998
Wydawca:
Politechnika Gdańska
Tematy:
conformational analysis
tachykinin peptides
NMR spectroscopy
Scyliorhinin II
molecular dynamics calculation methods
DMSO
Opis:
Conformational analysis of two tachykinin family peptides: Scyliorhinin I (ScyI) and Scyliorhinin II (ScyII) was carried out by 1D- and 2D-NMR (DQF-COSY, TOCSY, HMQC, HMBC, NOESY and ROESY) and molecular dynamics calculation methods in water and DMSO. Scyliorhinin I is an equipotent agonist of NK-1 and NK-2 tachykinin receptors and Scyliorhinin II is a selective agonist of the NK-3 tachykinin receptor. In DMSO, two groups of conformations (major and minor) were obtained for both peptides based on the experimental data. The conformations proposed for ScyI represent a folded structure, which show certain similarities to the structures reported for other NK-1 and NK-2 tachykinin agonists. In water ScyII displays a flexible, extended structure, whereas in DMSO the structure is more compact and in the fragment from the centre to the C-terminus several beta -turns may be present.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 1998, 2, 1; 47-53
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular modelling in the rational design of some anti-tumor and antifungal agents
Autorzy:
Mazerski, J.
Borowski, E.
Powiązania:
https://bibliotekanauki.pl/articles/1953949.pdf
Data publikacji:
1998
Wydawca:
Politechnika Gdańska
Tematy:
molecular modelling
molecular mechanics
molecular dynamics
semiempirical methods
antifungal agents
anti-tumor agents
rational design
Opis:
In this paper we present our approaches and results concerning application of molecular modelling techniques in the design of new chemotherapeutic agents for the control of eukariotic systems, comprising compounds for the treatment of systemic fungal infections and tumor deseases. In the case of anti-tumor agents we focused our attention on molecular properties of natural and synthetic anthraquinones. In the area of antifungal compounds we adopted two approaches. In one of them we examine molecular nature of undesirable properties of polyene macrolide antifungal antibiotic - amphotericin B using molecular modelling techniques. Another approach was aimed at the development of selective inactivator of glucosamine synthase, a novel target for antifungal compounds. In this problem we have used computational chemistry methods to identify structural features responsible for the selective inactivation of the target enzyme.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 1998, 2, 4; 511-550
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
2D simulations of liquid percolation through model porous media: preliminary MD and DPD results
Autorzy:
Rychcik, M.
Bośko, J.
Rybicki, J.
Alda, W.
Dzwinel, W.
Mancini, G.
Fioretti, L.
Powiązania:
https://bibliotekanauki.pl/articles/1964080.pdf
Data publikacji:
2001
Wydawca:
Politechnika Gdańska
Tematy:
2D flow simulations
particle methods
molecular dynamics
dissipative particle dynamics method
Opis:
In the paper we make a short overview of computer models based on particle approach, which can be suitable for the simulation of fluid flow through porous media. We concentrate on Molecular Dynamics (MD) and Dissipative Particle Dynamics (DPD) methods. We describe main features of our simulation programs, and present and discuss preliminary results of MD and DPD simulations of 2D fluid flow through a simple model rigid porous media. The paper aims at the evaluation of the applicability of MD and DPD methods for simulations of liquid flows in media of complicated geometry.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2001, 5, 1; 85-97
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Flows in microchannels
Autorzy:
Kucaba-Piętal, A.
Walenta, Z.
Peradzyński, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1955808.pdf
Data publikacji:
2001
Wydawca:
Politechnika Gdańska
Tematy:
microchannel flow
microflows
micropolar fluid
molecular dynamics simulations
direct Monte Carlo simulation
direct Monte Carlo methods
Opis:
The aim of this paper is to present a survey of the results for the flows of simple gases and liquids with substructure through narrow channels, obtained with the Direct Monte-Carlo and Molecular Dynamics Simulation methods.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2001, 5, 2; 179-189
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of the Glu342Lys mutation in α1-antitrypsin on its structure, studied by molecular modelling methods.
Autorzy:
Jezierski, Grzegorz
Pasenkiewicz-Gierula, Marta
Powiązania:
https://bibliotekanauki.pl/articles/1044164.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
serpins
protein structure
energy minimisation
molecular dynamics simulation
Opis:
The structure of native α1-antitrypsin, the most abundant protease inhibitor in human plasma, is characterised primarily by a reactive loop containing the centre of proteinase inhibition, and a β-sheet composed of five strands. Mobility of the reactive loop is confined as a result of electrostatic interactions between side chains of Glu342 and Lys290, both located at the junction of the reactive loop and the β structure. The most common mutation in the protein, resulting in its inactivation, is Glu342→Lys, named the Z mutation. The main goal of this work was to investigate the influence of the Z mutation on the structure of α1-antitrypsin. Commonly used molecular modelling methods have been applied in a comparative study of two protein models: the wild type and the Z mutant. The results indicate that the Z mutation introduces local instabilities in the region of the reactive loop. Moreover, even parts of the protein located far apart from the mutation region are affected. The Z mutation causes a relative change in the total energy of about 3%. Relatively small root mean square differences between the optimised structures of the wild type and the Z mutant, together with detailed analysis of 'conformational searching' process, lead to the hypothesis that the Z mutation principally induces a change in the dynamics of α1-antitrypsin.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 65-75
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Innovations in breeding procedures of winter oilseed rape for development of improved initial materials and the use of biotechnological methods in the Czech Republic
Innowacje w metodach hodowli rzepaku ozimego w Czechach dla uzyskania ulepszonego materiału wyjściowego oraz zastosowanie metod biotechnologicznych
Autorzy:
Kucera, V.
Vyvadilova, M.
Klima, M.
Koprna, R.
Machackova, I.
Curn, V.
Powiązania:
https://bibliotekanauki.pl/articles/833713.pdf
Data publikacji:
2005
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
innovation
plant breeding
procedure
winter oilseed rape
oilseed rape
development
initial material
biotechnological method
doubled haploid
hybrid breeding
self-incompatibility
disease resistance
frost resistance
molecular marker
Czech Republic
Źródło:
Rośliny Oleiste - Oilseed Crops; 2005, 26, 1
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania nad wystepowaniem Fasciola hepatica na wybranych obszarach Polski w oparciu o metody molekularne i serologiczne
The occurrence of Fasciola hepatica in chosen regions of Poland based on molecular and serological methods
Autorzy:
Kozak-Cieszczyk, M
Powiązania:
https://bibliotekanauki.pl/articles/839741.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przywry
Fasciola hepatica
choroby czlowieka
Galba truncatula
zywiciele posredni
parazytologia
pasozyty
choroby zwierzat
Opis:
Fasciolosis, caused by the liver fluke (Fasciola hepatica) is an important issue for both human and animal health. The disease evokes economic losses which are a consequence of impaired animal productivity leading to higher costs of meat and milk production, as well as liver condemnation. The goals of this thesis were to: (1) elaborate a molecular method - PCR for the detection of F. hepatica DNA in intermediate and definite hosts; (2) estimate the usefulness of a recombinated cysteine proteinase produced in E. coli in the form of inclusive bodies in serological diagnosis of F. hepatica infection in definite hosts, using an enzyme-linked immunosorbent assay (ELISA); (3) conduct field research on the prevalence of infection among intermediate and definitive hosts (cattle) in chosen regions of Poland, utilizing the elaborated methods. Based on the results obtained in this study, it was established that it is possible to detect F. hepatica DNA in the feces of definite hosts with the elaborated PCR method. The amplification of a 124 base pair tandem repeat allows the detection of fluke larval stages in intermediate hosts within 12 hours of exposure and F. hepatica infection in definite hosts (by the 5th week in rats, 8th week in sheep and 10th week in cattle). Therefore, the PCR test is more sensitive than traditional microscopic methods. Furthermore, it was determined that, the recombinated cysteine proteinase in the form of inclusive bodies, after solubillization exhibits antigenic properties of the native protein and the ELISA method based on this antigen may be useful as a tool for diagnosing fasciolosis in sheep and cattle, in both serum and milk samples. The test achieves a greater sensitivity and specificity than an ELISA based on native excretory-secretory antigens. The results of field research indicate that Fasciola hepatica is a frequent parasite of cattle in central and eastern Poland. The mean prevalence was 34.86% (±16.95) in all studied areas. The prevalence among intermediate hosts varied greatly (0–100%). The elaborated tests were proved to be valuable, mutually complementing diagnostic tools, applicable to different epidemiological situations.
Źródło:
Annals of Parasitology; 2006, 52, 2; 137-139
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania nad występowaniem Fasciola hepatica na wybranych obszarach Polski w oparciu o metody molekularne i serologiczne
The occurrence of Fasciola hepatica in chosen regions of Poland based on molecular and serological methods
Autorzy:
Kozak-Cieszczyk, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144329.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przywry
Fasciola hepatica
choroby czlowieka
Galba truncatula
zywiciele posredni
parazytologia
pasozyty
choroby zwierzat
Opis:
Fasciolosis, caused by the liver fluke (Fasciola hepatica) is an important issue for both human and animal health. The disease evokes economic losses which are a consequence of impaired animal productivity leading to higher costs of meat and milk production, as well as liver condemnation. The goals of this thesis were to: (1) elaborate a molecular method - PCR for the detection of F. hepatica DNA in intermediate and definite hosts; (2) estimate the usefulness of a recombinated cysteine proteinase produced in E. coli in the form of inclusive bodies in serological diagnosis of F. hepatica infection in definite hosts, using an enzyme-linked immunosorbent assay (ELISA); (3) conduct field research on the prevalence of infection among intermediate and definitive hosts (cattle) in chosen regions of Poland, utilizing the elaborated methods. Based on the results obtained in this study, it was established that it is possible to detect F. hepatica DNA in the feces of definite hosts with the elaborated PCR method. The amplification of a 124 base pair tandem repeat allows the detection of fluke larval stages in intermediate hosts within 12 hours of exposure and F. hepatica infection in definite hosts (by the 5th week in rats, 8th week in sheep and 10th week in cattle). Therefore, the PCR test is more sensitive than traditional microscopic methods. Furthermore, it was determined that, the recombinated cysteine proteinase in the form of inclusive bodies, after solubillization exhibits antigenic properties of the native protein and the ELISA method based on this antigen may be useful as a tool for diagnosing fasciolosis in sheep and cattle, in both serum and milk samples. The test achieves a greater sensitivity and specificity than an ELISA based on native excretory-secretory antigens. The results of field research indicate that Fasciola hepatica is a frequent parasite of cattle in central and eastern Poland. The mean prevalence was 34.86% (±16.95) in all studied areas. The prevalence among intermediate hosts varied greatly (0–100%). The elaborated tests were proved to be valuable, mutually complementing diagnostic tools, applicable to different epidemiological situations.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 2; 137-139
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody molekularne i immunologiczne stosowane w diagnostyce grzybic
Molecular and immunological methods applied in diagnosis of mycoses
Autorzy:
Kuba, K
Powiązania:
https://bibliotekanauki.pl/articles/841048.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka lekarska
diagnostyka molekularna
metody diagnostyczne
diagnostyka immunologiczna
metody molekularne
metoda nested-PCR
testy serologiczne
metoda EIA
metoda RT-PCR
metoda RAPD
markery molekularne
metoda RFLP
lancuchowa reakcja polimerazy
metoda AFLP
metody immunologiczne
grzybice
Źródło:
Annals of Parasitology; 2008, 54, 3; 187-197
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody molekularne i immunologiczne stosowane w diagnostyce grzybic
Molecular and immunological methods applied in diagnosis of mycoses
Autorzy:
Kuba, K.
Powiązania:
https://bibliotekanauki.pl/articles/2143775.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka lekarska
diagnostyka molekularna
metody diagnostyczne
diagnostyka immunologiczna
metody molekularne
metoda nested-PCR
testy serologiczne
metoda EIA
metoda RT-PCR
metoda RAPD
markery molekularne
metoda RFLP
lancuchowa reakcja polimerazy
metoda AFLP
metody immunologiczne
grzybice
Opis:
The diagnosis of fungal infections remains a problem for the management of fungal diseases, particularly in the immunocompromised patients. Systemic Candida infections and invasive aspergillosis can be a serious problem for individuals who need intensive care. Traditional methods used for the identification and typing of medically important fungi, such as morphological and biochemical analysis, are time−consuming. For the diagnosis of mycoses caused by pathogenic fungi faster and more specific methods, especially after the dramatic increase in nosocomial invasive mycoses are needed. New diagnostic tools to detect circulating fungal antigens in biological fluids and PCR−based methods to detect species or genus−specific DNA or RNA have been developed. Antigen detection is limited to searching only one genus. Molecular genetic methods, especially PCR analysis, are becoming increasingly important as a part of diagnostics in the clinical mycology laboratory. Various modifications of the PCR method are used to detect DNA in clinical material, particularly multiple, nested and real−time PCR. Molecular methods may be used to detection of nucleic acids of fungi in clinical samples, to identify fungal cultures at the species level or to evaluate strain heterogeneity differences within the species. This article reviews some of the recent advances in the possibility of molecular diagnosis of fungal infections.
Źródło:
Wiadomości Parazytologiczne; 2008, 54, 3; 187-197
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optyczne metody obrazowania molekularnego
Optical methods of molecular imaging
Autorzy:
Sołtysiński, T.
Liebert, A.
Zawicki, I.
Maniewski, R.
Powiązania:
https://bibliotekanauki.pl/articles/261383.pdf
Data publikacji:
2008
Wydawca:
Politechnika Wrocławska. Wydział Podstawowych Problemów Techniki. Katedra Inżynierii Biomedycznej
Tematy:
metody optyczne
obrazowanie molekularne
optical methods
molecular imaging
Opis:
Obrazowanie molekularne jest szybko rozwijającą się dziedziną badań w zakresie biotechnologii i inżynierii biomedycznej. W artykule przedstawiono przegląd technik stosowanych w obrazowaniu molekularnym, wykorzystujących metody medycyny nuklearnej oraz optyczne techniki oparte na analizie promieniowania fluorescencyjnego. W szczególności opisano metody optyczne obrazowania molekularnego stosowane w skali mikroskopowej (mikroskopia konfokalna, obrazowanie czasu relaksacji fluorescencyjnej, transfer energii Foerstera) oraz wykorzystywane w badaniach na zwierzętach doświadczalnych. Omówiono także potencjalne wykorzystanie technik optycznych w badaniach dużych objętości tkanek.
Molecular imaging (MI) is a rapidly emerging field of biomedical, biotechnological and engineering research. This study provides a brief review of the state-of-the-art techniques and methods of MI based on nuclear physics and fluorescent agents. Special attention will be focused on optical methods of MI applied in microscopic scale (multiphoton confocal microscopy, fluorescence lifetime imaging, Forster energy transfer) and in experimental animals. Potential application of MI in large tissue volumes will be also discussed.
Źródło:
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna; 2008, 14, 4; 331-335
1234-5563
Pojawia się w:
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Quantum-classical calculations of the nanomechanical properties of metals
Autorzy:
Dziedzic, J.
Powiązania:
https://bibliotekanauki.pl/articles/1964138.pdf
Data publikacji:
2009
Wydawca:
Politechnika Gdańska
Tematy:
hybric methods
cross-scaling
tight-binding
learn-on-the-fly
nanoscale
metals
nanoindentation
molecular-dynamics
Sutton-Chen potential
Opis:
Molecular-dynamics (MD) simulations constitute an important tool in the study of nanoscale metallic systems, especially so in the face of the difficulties plaguing their experimental analysis. Main limitations of the MD method stem from the empirical nature of the potentials employed, their functional form which is postulated ad hoc, and its classical nature. The neglect of electronic effects and the unjustified utilization of the potential for system configurations significantly different from those, for which the potential was parametrized makes the results of strictly classical calculations dubious, at least for a certain class of systems. On the other hand, high computational complexity of quantum-based methods, where atomic interactions are described ab initio, prohibits their direct use in the study of systems larger than several tens of atoms. In the last decade, a growing popularity of so-called hybrid (or cross-scaling) methods can be observed, that is, methods which treat the most interesting part of the system with a quantum-based approach, while the remainder is treated classically. Physically sound handshaking between the two methodologies (quantum and classical) within a single simulation constitutes a serious challenge, the majority of difficulties concentrating around the interface between the fragments of the system treated with the two methods. The aforementioned interface is most easily constructed for covalently bonded systems, where the bonds cut by the isolation of the quantum-based region can be saturated by the introduction of specially crafted link-atoms. In metallic systems, however, due to electronic delocalization, this traditional approach cannot be employed. This paper describes a physically sound and adequately efficient computational technique, which allows for the inclusion of results of locally employed quantum-based computations within a molecular-dynamics simulation, for systems described by the many-body Sutton-Chen (SC) potential, used in the study of fcc metals. The proposed technique was developed taking as a point of departure the Learn-on-the-Fly (LOTF) formalism, a recent development itself. The original LOTF approach is only suitable for two- or three-body potentials and is serial in nature, whereas the proposed technique can be used with many-body potentials and is parallel-ready. An implementation of the proposed approach in the form of computer code, which allows for parallel hybrid computations for metallic systems is also described. Finally, results from a set of hybrid simulations of nanoindentation of a copper workmaterial with a hard indenter utilizing the aforementoned technique and computer code is presented, as evidence of its viability.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2009, 13, 3; 207-310
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metod RAPD i ISSR do oceny podobieństwa genetycznego greckich form Dasypyrum villosum (L.) P. Candargy
Application of RAPD and ISSR methods to genetic similarity estimation of Greek Dasypyrum villosum (L.) P. Candargy populations
Autorzy:
Grądzielewska, Agnieszka
Paczos-Grzęda, Edyta
Gruszecka, Daniela
Powiązania:
https://bibliotekanauki.pl/articles/42809908.pdf
Data publikacji:
2009
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Dasypyrum
ISSR
RAPD
markery molekularne
podobieństwo genetyczne
genetic similarity
molecular markers
Opis:
W pracy przeprowadzono ocenę podobieństwa genetycznego 9 greckich form Dasypyrum villosum L. (P.) Candargy pochodzących z trzech regionów Grecji (Tesalii oraz Zachodniej i Środkowej Macedonii) na podstawie polimorfizmu markerów RAPD i ISSR. Analizę podobieństwa genetycznego przeprowadzono z wykorzystaniem 18 wyselekcjonowanych starterów RAPD i 17 starterów ISSR. Ogółem otrzymano 122 fragmenty RAPD i 210 ISSR, z czego 50% było polimorficznych. Technika RAPD pozwoliła na identyfikację produktów specyficznych jedynie u pięciu z dziewięciu badanych form, podczas gdy przy udziale metody ISSR otrzymano takie fragmenty dla siedmiu populacji, najwięcej dla W6 7264. Dla obu zastosowanych w badaniach metod obliczono współczynnik informacji o polimorfizmie (PIC), którego wartość zawierała się w przedziale 0,13–0,68 dla RAPD i 0,15–0,52 dla ISSR, średnio odpowiednio 0,32 i 0,33. Na podstawie polimorfizmu markerów RAPD i ISSR oraz obu technik łącznie obliczono wartości indeksów podobieństwa genetycznego Dice’a, pomiędzy parami badanych populacji. Średnie podobieństwo badanych obiektów wyniosło odpowiednio 0,854, 0,871 i 0,864. Najbardziej odmienną od pozostałych była forma W6 7264. W oparciu o matryce podobieństw Dice’a skonstruowano dendrogramy obrazujące stosunki pokrewieństwa pomiędzy badanymi populacjami.
Estimation of genetic similarity between nine Greek Dasypyrum villosum L. (P.) Candargy populations native to three Greece regions (Thesally, West and Central Macedonia) was performed basing on polymorphism of RAPD and ISSR markers. Eighteen RAPD and seventeen ISSR selected primers were used in the genetic similarity analyses. In total, 122 RAPD and 210 ISSR products were obtained, out of which 50% were polymorphic. The RAPD method identified specific products only in five from nine populations studied, while ISSR in seven, most for W6 7264. Polymorphic information content (PIC) values calculated for the both methods, ranged from 0.13–0.68 for RAPD and 0.15–0.52 for ISSR. Mean values of PIC for RAPD and ISSR were 0.32 and 0.33, respectively. Based on the polymorphism of RAPD and ISSR markers and both methods combined, genetic similarities using Dice algorithm were estimated between pairs of populations analyzed. Mean genetic similarities were 0.854, 0.871 and 0.864, respectively. Most different from the others was W6 7264. Based on Dice matrix similarities, dendrograms showing relatedness between the analyzed populations were constructed.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2009, 253; 297-307
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Progress in the molecular methods for the detection and genetic characterization of Cryptosporidium in water samples
Autorzy:
Skotarczak, B
Powiązania:
https://bibliotekanauki.pl/articles/52184.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
protozoan parasite
Cryptosporidium
genetic characteristics
molecular method
detection
water sample
food-borne way
oocyst
infection control
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 1-8
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie spektrometrii mas do analizy modyfikacji nukleotydów i adduktów DNA
Application of mass spectrometry methods for analysis of modified nucleotides and DNA adducts
Autorzy:
Hanus, J.
Jelonek, K.
Pietrowska, M.
Powiązania:
https://bibliotekanauki.pl/articles/171867.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
kancerogeneza
diagnostyka molekularna
spektrometria mas
nukleotydy
uszkodzenia DNA
addukty DNA
metylacja DNA
carcinogenesis
molecular diagnostics
mass spectrometry
nucleotides
DNA damage
DNA adducts
DNA methylation
Opis:
Chemically modified nucleotides, which are not normally present in genetic material, are called DN A adducts. This type of DN A modifications (damage) is directly related to processes of mutagenesis and carcinogenesis. Elevated levels of DN A adducts present in genetic material reflect exposure of humans to carcinogenic factors and are markers of increased risk of cancer [1]. For this reason different methods useful for quantitative and qualitative analyses of DN A adducts are used in the field of cancer prevention and research (Tab. 1). Enzymatically-catalyzed methylation of cytosine, observed mostly in so called CpG islands, is a frequent endogenous modification of genetic material. Such a DN A methylation is a key factor involved in regulation of gene expression, and methylation status of oncogenes and tumor supressor genes is an important biomarker of carcinogenesis. As such, analytical methods for assessment of DN A methylation are of great importance for molecular diagnostics of cancer. During the last decade significant progress has been made in methods available for quantitative, qualitative and structural analyses of biological molecules. Among intensively developed tools for bioanalyses are methods of mass spectrometry. Spectrometers that are based on two methods of ionization, namely electrospray ionization (ESI ) [30] and matrix-assisted laser desorption-ionization (MALDI ) [48], are particularly suitable for analyses of biological macromolecules: proteins and nucleic acids. Currently available mass spectrometers, together with microscale methods for sample preparation and separation, significantly increased sensitivity and accessible mass range of analyses. New generation of “user-friendly” instruments is developed to bring the techniques directly into the workplaces of biological and clinical investigators. This review demonstrates representative examples of mass spectrometry techniques used for qualitative analyses of nucleotide modifications and adducts present in genetic material of humans. In this field several methods base on spectrometers with electrospray ionization. Generated ions are separated according to their mass-to-charge ratio in an analyzer by electric fields; among different ion analyzers frequently used in this methods are single or triple quadrupole and ion traps (Fig. 1). Among other methods available for assessment of DN A adducts is so called Accelerator Mass Spectrometry (Fig. 2) [41]. The most frequently applied method for the assessment of DN A methylation is based on methylation-specific PCR reaction. Products of such PCR reactions are analyzed using MALDI mass spectrometry [54] (Fig. 3). In summary, new powerful methods of mass spectrometry that made available qualitative analyses of damage and modifications of human genetic material found their important place in modern biological and medical laboratories.
Źródło:
Wiadomości Chemiczne; 2011, 65, 3-4; 191-205
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 50

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