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Tytuł:
Microbial diversity of sewage sludge
Różnorodność mikrobiologiczna osadów ściekowych
Autorzy:
Błaszczyk, K.
Krzyśko-Łupicka, T.
Powiązania:
https://bibliotekanauki.pl/articles/125947.pdf
Data publikacji:
2013
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
sewage sludge
microbiological determination
microbial diversity
osad ściekowy
ocena mikrobiologiczna
różnorodność mikrobiologiczna
Opis:
Sewage sludge may be a cluster of different groups of microorganisms, including potential pathogenic, therefore processing of sludge should be conducted in a manner that protects workers and the environment from the sanitary risks. The aim of the study was to assess quantitatively and qualitatively the microbial diversity of the municipal sewage sludge, from the food industry and from the coke after the treatment, with the addition of flocculant and/or lime. Research material consisted of samples taken directly from the wastewater treatment plant. Quantitative and qualitative assessment of mesophilic and psychrophilic bacteria, yeasts, filamentous fungi and potentially pathogenic microorganisms was performed by a culture method. Techniques for treatment sewage sludge towards their hygenisation and reduce the amount of sediment by supporting flocculants and/or liming affect the quantitative and qualitative microorganisms composition. In the studied sewage sludge dewatered using a flocculant, with the exception of sediments from the food industry, there was the highest total number of microorganisms, both meso- and psychrophilic bacteria, yeast, filamentous fungi and potentially pathogenic bacteria. However, in the sediments of the food industry increase in the number of bacteria was observed only after simultaneous application of liming. Among the isolated microorganisms, depending on the origin of the sludge there was a domination of gram negative rods, with the exception of coke sediments without a flocculant. The municipal and from the food industry sediments were dominated by fungi: Aspergillus niger and Cladosporium sp., in coke sediments Aspergillus amstelodami, Paecilomyces javanicus and Acremonium sp. In these latter was also observed the greatest diversity of yeasts.
Osady ściekowe mogą być skupiskiem różnych grup mikroorganizmów, w tym potencjalnie chorobotwórczych, dlatego ich przeróbka powinna być prowadzona w sposób chroniący pracowników i środowisko przed zagrożeniem sanitarnym. Celem badań była mikrobiologiczna ocena ilościowa i jakościowa osadów ściekowych komunalnych i pochodzących z przemysłu spożywczego oraz koksowni po przeróbce z dodatkiem flokulantu i/lub wapna palonego. Materiał badawczy stanowiły próbki pobrane bezpośrednio z oczyszczalni ścieków. Ocenę ilościową i jakościową bakterii mezofilnych i psychrofilnych, drożdży, grzybów strzępkowych oraz mikroorganizmów potencjalnie chorobotwórczych przeprowadzono metodą hodowlaną. Techniki przeróbki osadów ściekowych w kierunku ich higienizacji oraz zmniejszenia ilości osadów poprzez wspomaganie flokulantami i/lub wapnowanie miały wpływ na skład ilościowy i jakościowy mikroorganizmów. W badanych osadach ściekowych odwadnianych flokulantem, z wyjątkiem osadów z przemysłu spożywczego, występowała najwyższa ogólna liczba mikroorganizmów, zarówno bakterii mezo-, jak i psychrofilnych, drożdży i grzybów strzępkowych oraz bakterii potencjalnie chorobotwórczych. Natomiast w osadach przemysłu spożywczego wzrost liczebności bakterii obserwowano dopiero po równoczesnym zastosowaniu wapnowania. Wśród wyizolowanych mikroorganizmów, w zależności od pochodzenia osadu ściekowego, stwierdzono dominację pałeczek gram-ujemnych z wyjątkiem osadów koksowniczych bez flokulantu. W osadach komunalnych i pochodzenia spożywczego dominowały grzyby: Aspergillus niger oraz Cladosporium sp., a w osadach koksowniczych Aspergillus amstelodami, Paecilomyces javanicus i Acremonium sp. W tych ostatnich obserwowano również największą różnorodność drożdży.
Źródło:
Proceedings of ECOpole; 2013, 7, 2; 461-466
1898-617X
2084-4557
Pojawia się w:
Proceedings of ECOpole
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of different soil management practices on soil properties and microbial diversity
Autorzy:
M. Gajda, Anna
A. Czyż, Ewa
R. Dexter, Anthony
M. Furtak, Karolina
Grządziel, Jarosław
Stanek-Tarkowska, Jadwiga
Powiązania:
https://bibliotekanauki.pl/articles/972380.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Opis:
The effects of different tillage systems on the properties and microbial diversity of an agricultural soil was investigated. In doing so, soil physical, chemical and biological properties were analysed in 2013-2015, on a long-term field experiment on a loamy sand at the IUNG-PIB Experimental Station in Grabów, Poland. Winter wheat was grown under two tillage treatments: conventional tillage using a mouldboard plough and traditional soil tillage equipment, and reduced tillage based on soil crushing-loosening equipment and a rigid-tine cultivator. Chopped wheat straw was used as a mulch on both treatments. Reduced tillage resulted in increased water content throughout the whole soil profile, in comparison with conventional tillage. Under reduced tillage, the content of readily dispersible clay was also reduced, and, therefore, soil stability was increased in the toplayers, compared with conventional tillage. In addition, the beneficial effects of reduced tillage were reflected in higher soil microbial activity as measured with dehydrogenases and hydrolysis of fluorescein diacetate, compared with conventional tillage. Moreover, the polimerase chain reaction – denaturing gradient gel electrophoresis analysis showed that soil under reduced tillage had greater diversity of microbial communities, compared with conventionally-tilled soil. Finally, reduced tillage increased organic matter content, stability in water and microbial diversity in the top layer of the soil.
Źródło:
International Agrophysics; 2018, 32, 1
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphology analysis and microbial diversity in novel anaerobic baffled reactor treating recycled paper mill wastewater
Autorzy:
Zwain, Haider M.
Al-Marzook, Farah A.
Nile, Basim K.
Jeddoa, Zuhair Mohammed Ali
Atallah, Aqeel H.
Dahlan, Irvan
Hassan, Waqed Hammed
Powiązania:
https://bibliotekanauki.pl/articles/2073763.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
anaerobic digestion
AD
novel anaerobic baffled reactor
NABR
recycled paper mill wastewater
RPMW
microbial diversity
microbial morphology
Opis:
The profile of microbial diversity in a NABR digesting RPMW was investigated using phylogenetic analysis of partial 16S rRNA sequences by a neighbor-joining-tree, supported by microbial morphology analysis by SEM. The results showed that microorganism inside NABR consisted of dominant Bacillus (25 strains) and Bacterium (1 strain) which were isolated from the settled sludge at the bottom of the reactor, whilst Bacillus (2 strains), Pseudomonas (2 strain) and Chryseobacterium (2 strain) were isolated from the biofilm formed on the packing material. It revealed that the microbial community strains, function, and structure changed simultaneously throughout the reactor system. The microscopic results showed rich biofacies, while the dominant microorganisms have various morphologies in every compartment of the system. It consisted of a long rod-shaped and filamentous bacterium composed majorly of bacilli of different sizes. Although the study successfully analyzed the microbial diversity and morphology in the system, the microbial communities reported in this study were different from other similar studies. This may be caused by the application of a culture-based technique that usually provides limited information due to the number of barely cultivated or uncultured strains.
Źródło:
Archives of Environmental Protection; 2021, 47, 4; 9--17
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbial contamination level and microbial diversity of occupational environment in commercial and traditional dairy plants
Autorzy:
Stobnicka-Kupiec, A.
Gołofit-Szymczak, M.
Górny, R.
Powiązania:
https://bibliotekanauki.pl/articles/2085184.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
occupational exposure
bacteria
fungi
bioaerosols
surface swabs
dairy plants
Opis:
Objective. The aim of this study was to assess microbial contamination of the air and surfaces at workplaces in commercial (CD) and traditional (TD) dairies. Materials and method. Bioaerosol (impactor) and surface (swab) samples were collected in CD and in TD. Bacterial and fungal concentrations in the air and on surfaces were calculated and all isolated microorganisms taxonomically identified, based on their morphological, biochemical and molecular features. Results. Average concentrations of bacterial aerosol ranged between 70–860 CFU/m3 and 265–14639 CFU/m3, while for fungal aerosol were between 50–290 CFU/m3 and 55–480 CFU/m3 in CD and TD, respectively. Average bacterial concentrations on surfaces ranged between 1.0–49.7 CFU/cm2 and 0.2–60.4 CFU/cm2, whereas average fungal surface contamination ranged between 0–2.7 CFU/cm2 and 0–4.6 CFU/cm2 in CD and TD, respectively. Qualitative analysis revealed mainly the presence of saprophytic microorganisms; however, several pathogenic strains (Staphylococcus aureus, Streptococcus intermedius, Clostridium perfringens, Actinomyces spp., Streptomyces spp., Candida albicans) were also isolated from both the air and surface samples in the studied dairies. Conclusions. The air and surfaces in TD were more polluted than those in CD; however, in both types of dairies, the levels of microbial contaminants did not exceed respective threshold limit values. Nevertheless, the presence of pathogenic microorganisms may increase health risk for dairy workers and influence the quality of products. Hence, proper hygienic measures should be introduced and performed to guarantee high microbial quality of both production processes and milk products.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 555-565
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Salivary microbial diversity – an investigation on possible biomarkers for polycystic ovarian syndrome from eastern India
Autorzy:
Ghosh, Kusum
Chakraborty, Shreyoshi
Chatterjee, Diptendu
Ratan Bandyopadhyay, Arup
Powiązania:
https://bibliotekanauki.pl/articles/29520460.pdf
Data publikacji:
2023-12-31
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:
diagnostic biomarker
PCOS
reproductive health
salivary microbiome
well-being
Opis:
Introduction and aim. Polycystic ovarian syndrome (PCOS) is one of the major reproductive health issues, thought to be multifactorial, needs serious attention as a dual burden (health and economic) mainly for developing countries like India, due to its rapid rise (30%) in the last couple of years. Therefore, widespread and liberal screening for this disorder towards prognosis, diagnosis and intervention seems to be an urgent area of research. In this background, the present study attempts to unravel the association of salivary microbial diversity and PCOS. Material and methods. To achieve the purpose 100 clinically diagnosed PCOS individuals and 110 age matched non-PCOS participants from Bengalee Hindu caste population, West Bengal, India was considered. Obtained salivary samples were identified with 16S rDNA amplification and microbial diversity were determined by Alu I restriction enzyme digestion. Results. The present study revealed an explicit pattern of DNA fragment lengths varied between 200 bp and 225 bp in PCOs in comparison to the non-PCOS group. Conclusion. The cardinal feature of the present study as the first attempt from India envisaged, utilization of salivary microbial diversity as an additional potential and economizing biomarker for PCOS that stimulate new horizon of research in 21st century’s anthropology – the anthropology of microbes.
Źródło:
European Journal of Clinical and Experimental Medicine; 2023, 4; 736-741
2544-2406
2544-1361
Pojawia się w:
European Journal of Clinical and Experimental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Non-lipophilic mycobiota of human skin
Autorzy:
Talaga, K.
Krzysciak, P.
Powiązania:
https://bibliotekanauki.pl/articles/67510.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
mycobiota
yeast
skin
human skin
microbial diversity
mold
Opis:
The human skin is inhabited by many species of bacteria and fungi, which are its natural microbiota. Fungi colonizing the skin, including those causing disease, characterized by great variety and variability, can be influenced by various factors. The purpose of this study was to investigate the composition of the non-lipiddependent fungal microbiota of skin, including the presence of species potentially pathogenic for humans. Fifty-six volunteers of both sexes aged 22–78 were subjected to the study. Swabs were taken from the face, chest, back and interdigital spaces of hands. Mycobiota isolated proved to vary both in terms of the location of occurrence and gender of patients. Interdigital spaces of hands, dominated by yeasts, constitute a location on human skin most contaminated with fungi. Molds were more often isolated from the face and chest. The back was the least contaminated location. There was no difference in fungal incidence in relation to sex.
Źródło:
Acta Mycologica; 2015, 50, 2
0001-625X
2353-074X
Pojawia się w:
Acta Mycologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects
Autorzy:
Otlewska, Anna
Adamiak, Justyna
Gutarowska, Beata
Powiązania:
https://bibliotekanauki.pl/articles/1039279.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
molecular techniques
microbial diversity
metagenomics
genetic fingerprinting
cultural heritage
biodeterioration
Opis:
As a result of their unpredictable ability to adapt to varying environmental conditions, microorganisms inhabit different types of biological niches on Earth. Owing to the key role of microorganisms in many biogeochemical processes, trends in modern microbiology emphasize the need to know and understand the structure and function of complex microbial communities. This is particularly important if the strategy relates to microbial communities that cause biodeterioration of materials that constitute our cultural heritage. Until recently, the detection and identification of microorganisms inhabiting objects of cultural value was based only on cultivation-dependent methods. In spite of many advantages, these methods provide limited information because they identify only viable organisms capable of growth under standard laboratory conditions. However, in order to carry out proper conservation and renovation, it is necessary to know the complete composition of microbial communities and their activity. This paper presents and characterizes modern techniques such as genetic fingerprinting and clone library construction for the assessment of microbial diversity based on molecular biology. Molecular methods represent a favourable alternative to culture-dependent methods and make it possible to assess the biodiversity of microorganisms inhabiting technical materials and cultural heritage objects.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 217-225
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Treatment of Industrial Textile Wastewater in Biological Aerated Filters – Microbial Diversity Analysis
Oczyszczanie ścieków włókienniczych w złożach zanurzonych – analiza bioróżnorodności drobnoustrojów
Autorzy:
Paździor, Katarzyna
Wrębiak, Julita
Ledakowicz, Stanisław
Powiązania:
https://bibliotekanauki.pl/articles/232988.pdf
Data publikacji:
2020
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
Biological Aerated Filters
metagenomic analysis
textile wastewater
toxicity
coagulation
biologiczne napowietrzane filtry
analiza metagenomiczna
ścieki tekstylne
toksyczność
koagulacja
Opis:
Investigated herein was the biodegradation of highly contaminated textile wastewater on a laboratory scale, with biological aerobic filters as a single treatment and in combination with the coagulation/flocculation process. Among the three support materials tested (Intalox saddles, ceramsite and beach shavings), the highest organic carbon compound removals (above 60% measured as COD and TOC) and steady operation were obtained for ceramsite. Effective and stable biological treatment was possible thanks to the development of biofilm of high bacterial and fungal diversity. The biodiversity of microflora was estimated on the basis of metagenomic analysis. The coagulation process with PAX 18 was effective in total phosphorus depletion (94%), while the coagulant Epoly CRD enabled up to 99% colour removal. The best results were obtained after the combined treatment, in which biodegradation was followed by coagulation (PAX 18). Such a combination enabled the removal of 98% of BOD5, 87% of COD, 88% of TOC, 48% of the total nitrogen, 98% of the total phosphorus, 98% of toxicity (towards Vibrio fisheri) and above 81% of colour.
Procesy wykończalnicze stosowane w przemyśle włókienniczym generują olbrzymie ilości ścieków, zaś zamykanie obiegów wody w farbiarniach jest najlepszym rozwiązaniem z punktu widzenia zrównoważonego rozwoju. Jedną z możliwości ponownego wykorzystania wody jest podział ścieków na dwa strumienie – mniej i bardziej obciążony zanieczyszczeniami. Strumień mniej obciążony może być, po odpowiednim oczyszczeniu zaawansowanymi metodami, zawrócony jako woda procesowa, natomiast strumień bardziej zanieczyszczony unieszkodliwiony. Niniejszy artykuł prezentuje wyniki unieszkodliwiania bardziej zanieczyszczonego strumienia za pomocą biodegradacji w złożach zanurzonych – samodzielnie oraz w połączeniu z koagulacją. Efektywność i stabilność procesów biologicznych została osiągnięta dzięki wpracowaniu biofilmu o wysokiej genotypowej bioróżnorodności – zarówno bakterii jak i grzybów. Bioróżnorodność mikroflory została oszacowana na podstawie analizy metagenomicznej. Najlepsze rezultaty zostały osiągnięte w sekwencji biodegradacja przed koagulacją. Taki układ umożliwił usunięcie ponad 98% BZT5, 87% ChZT, 88% OWO, 48% azotu całkowitego, 98% fosforu całkowitego, 98% toksyczności (w stosunku do bakterii Vibrio fisheri) oraz ponad 81% barwy.
Źródło:
Fibres & Textiles in Eastern Europe; 2020, 1 (139); 106-114
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Problematic Biases in the Availability of Molecular Markers in Protists: The Example of the Dinoflagellates
Autorzy:
Gómez, Fernando
Powiązania:
https://bibliotekanauki.pl/articles/763541.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Alveolate, Dinophyceae, Dinophyta, DNA barcoding, microbial diversity, molecular phylogeny, unicellular eukaryotes
Opis:
Dinoflagellates (Alveolata, Dinophyceae) are protists with a truly remarkable diversity in lifestyles (free-living, parasites and mutualistic symbionts), habitats (marine, freshwater, plankton, benthos), and trophic modes (heterotrophic, plastid-containing). Here dinoflagellates are used to evaluate biases in the availability of molecular markers in relation to the variety of functional and ecological characteristics of protists. A large number of dinoflagellate sequences are available in GenBank, at least one for 56% of the 264 described genera. The most common marker is the small ribosomal subunit ribosomal DNA (49%). At the species level, SSU rDNA or the large subunit rDNA are available for 15% of the 2,386 described species. Availability of sequences of the internal transcribed spacers (ITS) and cytochrome oxidase I (COI) show a strong bias towards cultivable species. Relative to trophic mode, while about half of the known dinoflagellates are heterotrophic, only 12% of them have been sequenced compared to 29% of the plastid-containing species. For the COI marker availability is 10 times greater for plastid-containing compared to heterotrophic species. Freshwater species are underrepresented (13%) relative to the marine forms (22%). A high proportion of benthic species have been sequenced (46%) reflecting interest in Symbiodinium and harmful epiphytic taxa. Most of the relatively few described mutualistic species have been sequenced (> 80%). In contrast, only 17% of the described parasitic species have been sequenced, and most of the available sequences were not identified at the species level. In recent years, new species have been described mostly from coastal blooms or cultures. These studies are favored by the availability of abundant material for detailed studies of ultrastructure and multi-gene molecular phylogenies. Many methods are difficult to apply for the scarce specimens available from the open ocean. The requirement of these protocols, easy to apply with cultured species, is an obstacle in our knowledge of the open ocean diversity because it discourages studies based on sparse material. Consequently, in recent years descriptions of new species from the open ocean have declined considerably.
Źródło:
Acta Protozoologica; 2014, 53, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody oparte o amplifikację DNA techniką PCR wykorzystywane w ocenie bioróżnorodności mikroorganizmów glebowych
Methods based on DNA PCR-amplification for evaluation of the soil microbial diversity
Autorzy:
Łyszcz, Małgorzata
Gałązka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1034148.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Tematy:
soil microorganism
genetic diversity
molecular markers
PCR based methods
ARDRA
PCR-RFLP
RAPD
REP-PCR
TRFLP
Opis:
Mikroorganizmy glebowe, pod względem cech genomowych i fenotypowych, stanowią wysoce zróżnicowaną grupę organizmów żywych. Z powodu tak dużej różnorodności ważne jest dobranie odpowiednich metod, dających największy stopień różnicowania mikroorganizmów. Narzędziami umożliwiającym analizę zmienności genetycznej mikroorganizmów są techniki genetyczne, a wśród nich jedną z najważniejszych jest łańcuchowa reakcja polimerazy, czyli PCR (Polymerase Chain Reaction), technika opracowana w latach 1980. Niniejsza praca stanowi przegląd podstawowych zagadnień dotyczących badania zmienności genetycznej mikroorganizmów glebowych w oparciu o markery molekularne z wykorzystaniem technik bazujących na reakcji PCR tj. PCR-RFLP, TRFLP, ARDRA, RAPD.
Soil microorganisms represent a highly diverse group of living organisms in terms of genomic and phenotypic characteristics. Due to such a large diversity, it is important to select appropriate identification methods which would secure its most complete determination. Genetic techniques are proper tools of choice for analyzing genetic variability of microorganism, the most important of which is the polymerase chain reaction (PCR), developed in the 1980s. This work presents an overview of the basic issues concerning studies on genetic variability of soil microorganisms with help of molecular markers and application of PCR techniques such as PCR-RFLP, TRFLP, ARDRA, RAPD.
Źródło:
Kosmos; 2017, 66, 2; 193-206
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbial functional diversity and enzymatic activity of soil degraded by sulphur mining reclaimed with various waste
Autorzy:
Joniec, Jolanta
Frąc, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/972819.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
functional diversity
β-glucosidase activity
degraded soil
recultivation
waste
Opis:
The aim of the study was to evaluate microbial functional diversity based on community level physiological profiling and b-glucosidase activity changes in soil degraded by sulphur mining and subjected to reclamation with various waste. The experiment was set up in the area of the former ‘Jeziórko’ Sulphur Mine (Poland), on a soilless substrate with a particle size distribution of slightly loamy sand. The experimental variants included the application of post-flotation lime, sewage sludge and mineral wool. The analyses of soil samples included the assessment of the following microbiological indices: b-glucosidase activity and functional diversity average well color development and richness). The results indicate that sewage sludge did not exert a significant impact on the functional diversity of microorganisms present in the reclaimed soil. In turn, the application of other types of waste contributed to a significant increase in the parameters of total metabolic activity and functional diversity of the reclaimed soil. However, the temporal analysis of the metabolic profile of soil microorganisms demonstrated that a single application of waste did not yield a durable, stable metabolic profile in the reclaimed soil. Still, there was an increase in β-glucosidase activity, especially in objects treated with sewage sludge.
Źródło:
International Agrophysics; 2017, 31, 4; 465-473
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity and functional diversity of microbial communities in long-term hydrocarbon and heavy metal contaminated soils
Aktywność i bioróżnorodność funkcjonalna zespołów mikroorganizmów w glebach długotrwale skażonych węglowodorami oraz metalami ciężkimi
Autorzy:
Markowicz, A.
Płaza, G.
Piotrowska-Seget, Z.
Powiązania:
https://bibliotekanauki.pl/articles/205111.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polycyclic aromatic hydrocarbons
heavy metals
microbial activity
functional diversity
community level physiological profiles
CLPPs
policykliczne węglowodory aromatyczne
metale ciężkie
aktywność mikrobiologiczna
bioróżnorodność funkcjonalna
profil fizjologiczny zespołów mikroorganizmów
Opis:
The impacts of long-term polycyclic aromatic hydrocarbons (PAHs) and heavy metal pollution on soil microbial communities functioning were studied in soils taken from an old coke plant. The concentrations of PAHs in the tested soils ranged from 171 to 2137 mg kg-1. From the group of tested heavy metals, concentrations of lead were found to be the highest, ranging from 57 to 3478 mg kg-1, while zinc concentrations varied from 247 to 704 mg kg-1 and nickel from 10 to 666 mg kg-1. High dehydrogenase, acid and alkaline phosphatase activities were observed in the most contaminated soil. This may indicate bacterial adaptation to long-term heavy metal and hydrocarbon contamination. However, the Community Level Physiological Profiles (CLPPs) analysis showed that the microbial functional diversity was reduced and influenced to a higher extent by some metals (Pb, Ni), moisture and conductivity than by PAHs.
Celem pracy było zbadanie wpływu wieloletniego skażenia gleb metalami ciężkimi oraz wielopierścieniowymi węglowodorami aromatycznymi (WWA) na funkcjonowanie zespołów mikroorganizmów. Do badań pobrano gleby z terenu koksowni, gdzie produkcja koksu trwa od 1884 roku. Stężenia WWA w badanych glebach wahały się w przedziale od 171 do 2137 mg kg-1. Wśród badanych metali zawartość ołowiu wynosiła od 57 do 3478 mg kg-1, niklu od 10 do 666 mg kg-1, a stężenie cynku od 247 do 704 mg kg-1. W najsilniej skażonej glebie zaobserwowano wysoką aktywność dehydrogenazy, fosfatazy kwaśnej i zasadowej. Wyniki takie mogą wskazywać na adaptację zespołów mikroorganizmów do długoletniego zanieczyszczenia metalami i WWA. Jednocześnie analiza profili fizjologicznych zespołów mikroorganizmów (CLPPs) wykazała obniżenie indeksu bioróżnorodności funkcjonalnej. Zaobserwowano również, że obecność metali (Pb, Ni) oraz parametry glebowe takie jak wilgotności i przewodności wywierały istotniejszy wpływ na bioróżnorodność metaboliczną niż WWA.
Źródło:
Archives of Environmental Protection; 2016, 42, 4; 3-11
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of activated sludge storage before RNA isolation
Autorzy:
Cydzik-Kwiatkowska, A.
Wnuk, M.
Powiązania:
https://bibliotekanauki.pl/articles/81045.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
activated sludge
biomass
degradation
microbial activity
molecular technique
optimization
population diversity
RNA isolation
RNA molecule
total suspended solid
waste water treatment plant
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbial biodiversity in arable soils is affected by agricultural practices
Autorzy:
Wolińska, Agnieszka
Górniak, Dorota
Zielenkiewicz, Urszula
Goryluk-Salmonowicz, Agata
Kuźniar, Agnieszka
Stępniewska, Zofia
Błaszczyk, Mieczysław
Powiązania:
https://bibliotekanauki.pl/articles/972735.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
dgge
16s rrna gene
simpson diversity
bacterial communities
arable soils
Opis:
The aim of the study was to examine the differences in microbial community structure as a result of agricultural practices. Sixteen samples of cultivated and the same number of non-cultivated soils were selected. Gel bands were identified using the GelCompar software to create the presence-absence matrix, where each band represented a bacterial operational taxonomic unit. The data were used for principal-component analysis and additionally, the Shannon-Weaver index of general diversity, Simpson index of dominance and Simpson index of diversity were calculated. Denaturing gradient gel electrophoresis profiles clearly indicated differentiation of tested samples into two clusters: cultivated and non-cultivated soils. Greater numbers of dominant operational taxonomic units (65) in non-cultivated soils were noted compared to cultivated soils (47 operational taxonomic units). This implies that there was a reduction of dominant bacterial operational taxonomic units by nearly 30% in cultivated soils. Simpson dominance index expressing the number of species weighted by their abundance amounted to 1.22 in cultivated soils, whereas a 3-fold higher value (3.38) was observed in non-cultivated soils. Land-use practices seemed to be a important factors affected on biodiversity, because more than soil type determined the clustering into groups.
Źródło:
International Agrophysics; 2017, 31, 2; 259-271
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metagenomic 16s rRNA investigation of microbial communities in the Black Sea estuaries in South-West of Ukraine
Autorzy:
Bobrova, Oleksandra
Kristoffersen, Jon
Oulas, Anastasis
Ivanytsia, Volodymyr
Powiązania:
https://bibliotekanauki.pl/articles/1038820.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
prokaryotic diversity
Black Sea estuaries
16S rDNA analysis
metagenomic analysis
Opis:
The Black Sea estuaries represent interfaces of the sea and river environments. Microorganisms that inhabit estuarine water play an integral role in all biochemical processes that occur there and form unique ecosystems. There are many estuaries located in the Southern-Western part of Ukraine and some of them are already separated from the sea. The aim of this research was to determine the composition of microbial communities in the Khadzhibey, Dniester and Sukhyi estuaries by metagenomic 16S rDNA analysis. This study is the first complex analysis of estuarine microbiota based on isolation of total DNA from a biome that was further subjected to sequencing. DNA was extracted from water samples and sequenced on the Illumina Miseq platform using primers to the V4 variable region of the 16S rRNA gene. Computer analysis of the obtained raw sequences was done with QIIME (Quantitative Insights Into Microbial Ecology) software. As the outcome, 57970 nucleotide sequences were retrieved. Bioinformatic analysis of bacterial community in the studied samples demonstrated a high taxonomic diversity of Prokaryotes at above genus level. It was shown that majority of 16S rDNA bacterial sequences detected in the estuarine samples belonged to phyla Cyanobacteria, Proteobacteria, Bacteroidetes, Actinobacteria, Verrucomicrobia, Planctomycetes. The Khadhzibey estuary was dominated by the Proteobacteria phylum, while Dniester and Sukhyi estuaries were characterized by dominance of Cyanobacteria. The differences in bacterial populations between the Khadzhibey, Dniester and Sukhyi estuaries were demonstrated through the Beta-diversity analysis. It showed that the Khadzhibey estuary's microbial community significantly varies from the Sukhyi and Dniester estuaries. The majority of identified bacterial species is known as typical inhabitants of marine environments, however, for 2.5% of microbial population members in the studied estuaries no relatives were determined.
Źródło:
Acta Biochimica Polonica; 2016, 63, 2; 315-319
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł

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