Wszystkie pola: in vitro cultivation - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Content of pyrrolizidine alkaloids (senecionine and senkirkine) in Tussilago farfara L. plants cultivated in vitro
Zawartość alkaloidów pirolizydynowych (senecjoniny i senkirkiny) w roślinach podbiału pospolitego hodowanego w kulturach in vitro
Autorzy:: Dreger, M.
Krajewska-Patan, A.
Gorska-Paukszta, M.
Pieszak, M.
Opala, B.
Gryszczynska, A.
Adamczak, A.
Mikolajczak, P.M.
Buchwald, W.
Powiązania:: https://bibliotekanauki.pl/articles/71169.pdf
Data publikacji:: 2012
Wydawca:: Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:: alkaloid content
pyrrolizidine alkaloid
senecionine
senkirkine
Tussilago farfara
plant cultivation
in vitro cultivation
Opis:: Tussilago farfara L. (family Asteraceae) is a valuable medicinal plant that has been used as a cough suppressant and as an antibacterial and anti-inflammatory drug. Mucopolysac charides, flavonoids, sterols, phenolic acids and pyrrolizidine alkaloids (PAs) are the main active compounds of coltsfoot. Due to hepatotoxic properties of some pyrrolizidine alkaloids, raw materials that contain PAs should be monitored and determined. The aim of present work was to establish nodal cultures of Tussilago farfara and to determine the content of senecionine and senkirkine in plants propagated in in vitro conditions. Eleven clones of coltsfoot derived from Polish natural populations were established. Rhizome buds were used as explants for the initiation of in vitro cultures on MS (Murashige and Skooge) medium. Every six weeks the shots and leaves were collected and dried. The HPLC method was applied for the identification and determination of senecionine and senkirkine. Content of pyrrolizidine alkaloids varied significantly depending on origin (population). An average sum of alkaloids (senecionine and senkirkine) ranged from 1.23 to 10.47 mg/100g d.w. that corresponds to 0.0013–0.011%, respectively.
Liść podbiału pospolitego (Tussilgo farfara L.) jest cenionym surowcem zielarskim, stosowanym w stanach zapalnych i nieżytach górnych dróg oddechowych oraz jako środek przeciwkaszlowy i przeciwbakteryjny. Za główne substancje aktywne surowca (liście) uważa się przede wszystkim mukopolisacharydy, ponadto podbiał zawiera także flawonoidy, sterole, kwasy fenolowe oraz hepatotoksyczne alkaloidy pirolizydynowe, jak senkirkinę i senecjoninę. Celem pracy było założenie kultur węzłowych podbiału pospolitego oraz określenie zawartości senecjoniny i senkirkiny w namnażanych roślinach. W rezultacie otrzymano 11 klonów podbiału pochodzących z różnych populacji z terenu Polski. Do inicjacji kultur węzłowych użyto pączków bocznych (z kłączy) na pożywce wg Murashige i Skooga (1962) bez dodatku regulatorów wzrostu. Co sześć tygodni zbierano ulistnione pędy otrzymanych roślin, które następnie ważono i suszono w temperaturze pokojowej. Identyfikacji tożsamości i zawartości alkaloidów pirolizydynowych dokonano przy użyciu metody HPLC. Zawartość senkirkiny i senecjoniny wahała się w zależności od populacji. Średnia zawartość sumy alkaloidów (senkirkiny i senecjoniny) wynosiła od 1,23 do 10,47 mg na 100 g suchej masy, co odpowiada zakresowi od 0,0013% do 0,011%.
Źródło:: Herba Polonica; 2012, 58, 4
0018-0599
Pojawia się w:: Herba Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Cultivation of soapwort plants (Saponaria officinalis L.) in in vitro cultures
Autorzy:: Figas, Anna
Lefelbajn, Weronika
Powiązania:: https://bibliotekanauki.pl/articles/16243498.pdf
Data publikacji:: 2023
Wydawca:: Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:: medicinal plant
axillary shoots
micropropagation
plant growth regulator
Opis:: Saponaria officinalis L. is a medicinal plant from the family Caryophyllaceae. It occurs in most European countries, as well as in North Africa, America and Western Asia. The aim of the study was to find an optimal method of sterilization S. officinalis seeds in order to obtain sterile seedlings and to multiply axillary shoots from nodal explants isolated from them, on MS medium with the addition of growth regulators (RW). In this study, 4 variants of seed sterilization were compared using NaClO at the following concentrations: 1.5% (2), 2% (3) and 2.5% (4) for a period of 11 minutes. The control was a variant in which 70% C2H5OH was used for pre-sterilization for 1 minute (1). The highest percentage of sprouted, seeds and sterile seedlings and the lowest percentage of contamination were obtained in variant (4). As a result of micropropagation of soapwort in in vitro cultures, axillary shoots were formed from nodal explants from axillary buds. The highest percentage of explants with shoots (95%) and callus tissue (48%) and the highest number of shoots from one explant (5.95) were obtained in the second passage on MS medium with the addition of 4 mg.dm-3 BAP and 0.5 mg.dm-3 NAA. Due to the wide possibilities of using soapwort, it is advisable to continue research aimed at developing an optimal and efficient plant regeneration system of this species.
Źródło:: Infrastruktura i Ekologia Terenów Wiejskich; 2023, I/1; 59--69
1732-5587
Pojawia się w:: Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Environmental and ecological aspects of cultivation of selected energy and herbal plants propagated by in vitro culture
Autorzy:: Tomaszewska-Sowa, M.
Figas, A.
Powiązania:: https://bibliotekanauki.pl/articles/61499.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:: environmental aspect
ecological aspect
plant cultivation
herbal plant
micropropagation
in vitro culture
growth regulation
Silphium perfoliatum
Helichrysum arenarium
Stevia rebaudiana
Opis:: There has been a growing interest over the last years in techniques using alternative and pharmaceutical plants due to their wide potential applications. These species are characterised by valuable and multidirectional usable features, due to which their practical application is superior to their physiognomic features. These plants may provide a raw material for production of medicines, cosmetics or may be used for animal feeds. Moreover they may be used as ornamental, melliferous and energy plants. Owing to their generally low soil requirements they are used in the reclamation of degraded land. Among the species characterized by multidirectional use are Silphium perfoliatum, Helichrysum arenarium and Stevia rebaudiana. These plants are characterized by a high content of active compounds and a number of pro-health characteristics. Because raw materials for production of medicines and cosmetics should be standardized, cultures maintained under controlled conditions are a good source of seedlings.
Źródło:: Infrastruktura i Ekologia Terenów Wiejskich; 2014, IV/3
1732-5587
Pojawia się w:: Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts
Autorzy:: Emsen, B.
Dogan, M.
Powiązania:: https://bibliotekanauki.pl/articles/11887732.pdf
Data publikacji:: 2018
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
Ceratophyllaceae
hornwort
Ceratophyllum demersum
plant propagation
in vitro propagation
plant regeneration
plant extract
antioxidant activity
methanol extract
metal chelating
medicinal use
reducing power
Opis:: Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 23-33
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Fast direct regeneration of plants from nodal explants of Stevia rebaudiana Bert.
Autorzy:: Doliński, R.
Kowalczyk, K.
Powiązania:: https://bibliotekanauki.pl/articles/12611010.pdf
Data publikacji:: 2019
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
Stevia rebaudiana
plant regeneration
plant use
medicinal plant
nodal explant
micropropagation
in vitro culture
auxin concentration
Opis:: In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg.dm–3). The plants were obtained as soon as after three weeks. The best results were obtained from media with various concentrations of the indole-3-acetic acid (IAA) and the highest concentration of phenylacetic acid (PAA). Plants were formed by 83.9–86.0% of explants, they had high weight (234–253 mg), two shoots measuring 2.07–2.37 cm and 5.8–8.3 roots measuring 1.00–1.24 cm. Mean plant weight was the lowest on media with indole-3-butyric acid (IBA) (185–192 mg). Both explant buds formed single shoots, but their development was typically uneven. The differences in the length and weight of shoots were the lowest on media with IAA and at lower PAA concentrations. Plants from the media with IAA and the control medium were distinguished by higher number of nodes. The percentage share of shoots in the total plant weight was the highest on media with PAA (62.1–62.7%), and the lowest at higher concentrations of α-naphthaleneacetic acid (NAA) (47.9 and 48.9%). Parts of explants immersed in media developed callus, and the highest amounts of this tissue were found in the media with NAA. 92.3% of plants survived the acclimatization. The applied procedure may be used for rapid in vitro cloning of selected stevia genotypes. The use of one medium enables reduction of seedling production costs. Moreover, cyclical cloning and extending the production scale is possible.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 95-103
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: In vitro multiplication and acclimatization of Biscutella laevigata (Brassicaceae) to cultivation in greenhouse conditions
Autorzy:: Hanus-Fajerska, E.
Wiszniewska, A.
Muszynska, E.
Powiązania:: https://bibliotekanauki.pl/articles/80691.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Biscutella laevigata
biostimulator
in vitro
propagation
multiplication
micropropagation
cultivation
greenhouse condition
vegetative propagation
photosynthetic photon flux density
plant growth regulator
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems
Autorzy:: Kaviani, B.
Bahari, B.
Powiązania:: https://bibliotekanauki.pl/articles/12678778.pdf
Data publikacji:: 2019
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
Gentianaceae
Eustoma
Lisianthus
Eustoma grandiflorum
ornamental plant
flowering plant
rooting
microshoot
micropropagation
in vitro proliferation
ex vitro method
soild phase
liquid phase
double-phase culture
plant growth regulator
auxin
cytokinin
Opis:: A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 47-56
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: In vivo confocal microscopy and in vitro culture techniques as tools for evaluation of severe Acanthamoeba keratitis incidents
Autorzy:: Padzik, Monika
Szaflik, Jacek P.
Baltaza, Wanda
Perkowski, Konrad
Dybycz, Monika
Chomicz, Lidia
Powiązania:: https://bibliotekanauki.pl/articles/972158.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: Acanthamoeba keratitis
in vitro cultivation
in vivo confocal microscopy
Opis:: Amphizoic amoebae belonging to the genus Acanthamoeba are known as etiological agents of sightthreatening Acanthamoeba keratitis. The leading risk factor for the development of this serious human disease is contact lens wearing which popularity increases worldwide, also in Poland. The disease with active epithelial inflammations, corneal ulcers, including loss of the visual acuity is a serious medical problem as an emerging threat for the public health related to improper contact lens hygiene. The treatment of the amoebic keratitis is difficult, often unsuccessful due to delayed proper diagnosis. The clinical picture of the disease, often with severe course is nonspecific, similar to that occurring in viral, fungal or bacterial keratitis, thus clinical symptoms alone are not sufficient to identify the causative agent of the amoebic infection. Early diagnosis is decisive for the suitable therapeutic management and the treatment efficacy. In our studies, several complicated, difficult to treat Acanthamoeba keratitis incidences pertaining Polish patients using contact lenses have been retrospectively analyzed in terms of the usefulness of non-invasive methods of in vivo confocal microscopy and in vitro culture techniques applied for diagnosis. Hyper-reflective double-walled spherical Acanthamoeba cysts, with a more reflective outer wall were detected in the epithelium and anterior layers of the corneal stroma. In vivo confocal microscopy, if available, may be a valuable, sensitive tool for diagnosis in late identified severe infections mainly with strong viability strains, however confoscan may offer limited value at lowintensity amoebic infections. The microscopic visualization of amoebae in slides prepared directly from corneal scraping and laboratory examinations of specimens from in vitro cultivated corneal isolates allow to confirm or verify results of in vivo examinations, furthermore to identify directly the pathogens and to clarify previous misdiagnoses.
Źródło:: Annals of Parasitology; 2017, 63, 4; 341-346
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Intra-strains diversity of expression of polymorphic PKS4 gene in comparison in zearalenone production by Fusarium graminearum during in vitro cultivation
Autorzy:: Misiewicz, Anna
Goncerzewicz, Anna
Jędrzejczak, Renata
Zdziennicki, Filip
Powiązania:: https://bibliotekanauki.pl/articles/1038847.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Fusarium sp. PKS4
ZEA
gene expression
polymorphism
Opis:: Filamentous fungi belonging to the Fusarium genus are responsible for large economic losses due to their high pathogenicity and toxigenicity. Fusarium sp. may produce variety of mycotoxins, one of them is zearalenone (ZEA). The presence of the PKS4 gene shows the possibility of zearalenone biosynthesis by Fusarium sp. In this study, in four Fusarium graminearum and one Fusarium poae strains the presence of PKS4 genes and ZEA concentrations were determined. The presence of the PKS4 gene was confirmed by classical polymerase chain reaction (PCR) in three of four strains of F. graminearum. One strain with no PKS4 gene detected was found while still producing ZEA. In the present study, a real-time PCR assay has been successfully performed for the relative expression of Fusarium strains based on new designed primers targeting the PKS4 gene involved in ZEA biosynthesis. Result shows that P56/4 strain of F. graminearum has the highest mRNA level, in the range of 12, what correlates to the high production of this mycotoxin. In this study, a real-time PCR assay has been successfully developed for the prediction of the production of ZEA by F. graminearum strains by PCR real-time techniques based on primers targeting the gene, PKS4, involved in ZEA biosynthesis. The special significance was pointed to occurring genes polymorphism.
Źródło:: Acta Biochimica Polonica; 2016, 63, 1; 97-102
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Sustainable micropropagation of selected Stevia rebaudiana Bertoni genotypes
Autorzy:: Kaplan, B.
Duraklioglu, S.
Turgut, K.
Powiązania:: https://bibliotekanauki.pl/articles/12691030.pdf
Data publikacji:: 2019
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: Turkey
plant cultivation
Compositae
Stevia rebaudiana
steviol glycoside
sweetener
micropropagation
in vitro propagation
growth regulator
genotype
Gelrite preparation
Opis:: Stevia rebaudiana Bertoni is a perennial plant belonging to Asteraceae family and its leaves contain steviol glycosides (SGs) that are 150 to 300 times sweeter than sucrose. The sweeteners obtained from S. rebaudiana can be safely used by diabetics as insulin secretion is not required during digestion of this sweetener. As it has zero calories, it is also used in diet products. Adaptation studies for Stevia conducted in Antalya, Turkey have shown that the stevia plant could easily be cultivated as a perennial. However, the lack of a sustainable vegetative propagation method creates a significant problem for stevia production. In the generatively populations, homogeneity and therefore quality are decreased because of cross-pollination. Stevia, as a self-incompatible and cross-pollinated species, has been shown to have very high genetic diversity. Therefore, development of a sustainable in vitro propagation method to prevent genetic heterogeneity of selected varieties is crucial for stevia cultivation. The aim of this study was to evaluate 2 different gelling agents (plant agar and Gelrite) and 20 different growth regulators combinations. The results demonstrated an approximately 200-fold multiplication rate obtained within 13 weeks using MS medium supplemented with 0.5 mg·dm–3 BAP and 0.25 mg·dm–3 kinetin and solidified with Gelrite. Average stevioside and rebaudioside A contents in in vitro propagated plant samples were found to be 8.1% and 8.6%, respectively.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 47-56
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: The effect of growth retardants applied in vitro on the acclimatization and growth of Tibouchina urvilleana Cogn. in vivo
Wpływ retardantów wzrostu zastosowanych in vitro na aklimatyzację i wzrost Tibouchina urvilleana Cogn. w warunkach in vivo
Autorzy:: Kozak, D
Powiązania:: https://bibliotekanauki.pl/articles/11364548.pdf
Data publikacji:: 2006
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: in vitro
acclimatization
growth retardant
plant cultivation
growth
in vivo
Tibouchina urvilleana
Opis:: Shoot tips of Tibouchina urvilleana Cogn. were cultured 4 weeks in vitro in modified Murashige and Skoog (MS) [1962] medium supplemented with growth retardants: paclobutrazol – 0.1, 0.5, 1.0, 5.0 mg·dm⁻³, flurprimidol – 0.1, 1.0, 5.0 mg·dm⁻³, chlorocholine chloride (CCC) – 5.0, 50.0, 250.0 mg·dm⁻³. Explants cultured on the medium without growth substances were used as a control. Rooted microcuttings were transferred to the greenhouse and transplanted into a mixture of 1 peat : 1 perlite, where they were grown for 5 weeks. Plants were then cultivated in a peat substrate during another 5 weeks. Acclimatization of rooted shoots in the greenhouse was affective in 92.5–100%. The survival of plants was lowest when microcuttings were previously cultured on medium with flurprimidol at 5.0 mg·dm⁻³. Cultivation of Tibouchina urvilleana shoots in vitro on media with various growth retardants had a significant effect on the further growth of plants ex vitro. Paclobutrazol and flurprimidol at 5.0 mg·dm⁻³ inhibited very strong growth of plants after 10 weeks of growth ex vitro.
Wierzchołki pędów Tibouchina urvilleana Cogn. prowadzono 4 tygodnie in vitro na zmodyfikowanej pożywce Murashige i Skooga (MS) [1962] z dodatkiem retardantów wzrostu: paklobutrazol – 0,1; 0,5; 1,0; 5,0 mg·dm⁻³, fluropirimidol – 0,1; 1,0; 5,0 mg·dm⁻³, chlorek chlorocholiny (CCC) – 5,0; 50,0; 250,0 mg·dm⁻³. Kontrolę stanowiła pożywka nie zawierająca retardantów wzrostu. Ukorzenione pędy przenoszono do szklarni i sadzono do mieszaniny torfu i perlitu (1:1), gdzie rosły przez 5 tygodni. Następnie rośliny przesadzono do substratu torfowego i uprawiano przez kolejne 5 tygodni. Rośliny przyjęły się w 92,5–100,0%. Najniższy procent przyjęć wykazywały rośliny pochodzące z pożywki zawierającej 5,0 mg·dm⁻³ fluropirimidolu. Wzrost in vitro pędów Tibouchina urvilleana na pożywce zawierającej retardanty wzrostu miał istotny wpływ na dalszy wzrost roślin w podłożu. Paklobutrazol i fluropirimidol w stężeniu 5,0 mg·dm⁻³ bardzo silnie hamowały wzrost roślin ex vitro po 10 tygodniach.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2006, 05, 1; 65-70
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
Autorzy:: Kozak, D.
Parzymies, M.
Świstowska, A.
Marcinek, B.
Ismael, B.S.
Powiązania:: https://bibliotekanauki.pl/articles/12663898.pdf
Data publikacji:: 2019
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
Brazilian jasmine
Mandevilla sanderi
ornamental plant
flowering plant
pot plant
commercial plant
plant growth
plant development
plant regeneration
explant type
multiplication
decapitation
defoliation
in vitro culture
tissue culture
cultivation experiment
Opis:: Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
Autorzy:: Marcinek, B.
Parzymies, M.
Poniewozik, M.
Kozak, D.
Durlak, W.
Powiązania:: https://bibliotekanauki.pl/articles/12307379.pdf
Data publikacji:: 2019
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
Dahlia
Dahlia x cultorum
dahlia
ornamental plant
flowering plant
tuberous plant
tuberous root
plant acclimatization
growth regulator
cytokinin
gibberellic acid
micropropagation
tissue culture
ex vitro method
microcutting
Opis:: The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Wyniki hodowli tasiemców z rzędu Psudophyllidea in vitro
Conditions of Psudophyllidea tapeworm cultivation in vitro
Uslovija rosta cestod iz roda Psudophyllidea in vitro
Autorzy:: Machnicka-Roguska, B.
Powiązania:: https://bibliotekanauki.pl/articles/836915.pdf
Data publikacji:: 1961
Wydawca:: Polskie Towarzystwo Parazytologiczne
Opis:: The review covers previous publications of Smyth dealing with cultivation of larval Cestode in vitro. Plecercoides were rearing in axenic conditions in vitro and obtained after 72 hours a mature from of Schistocephalus solidus. The plerocercoids were kept in a tube made of half permeable membrane which was placed in a vessel with fluent medium - horse serum; above vessel was heated to a temperature of 40° C being all the time shaked. The rearing of the coracides, out of Schistocephalus solidus ova obtained in vitro, procecded in water with a temperature or 25° C. The incubation of the coracides took place after 8 days. When the culture was trated with sun rays for several hours the process of the ova operculi opening was accelerated. The best results in cultivating plerocercoids Diphyllobothrium dendriticum in vitro up to a mature from was obtained on a medium containing an extract of duck embryo together with serum, monosacharides and albuminous hydrolysates. The plerocercoids after 6 days of breeding attained the stadium of late gametogenesis and shoved a defective somatic development. Any enrichment of the medium in nutritive substances failed to evoke further progress in the plerocercoids development - viz. formation of vitellous glands. In order to check whether the methods chosen for the development of tapeworms reared in vitro have been correct, cytologie, histological and histochemical tests have been performed. Cultivation in vitro is a contribution to the better understanding of relations and dependency existing between the parasite and its host.
Źródło:: Annals of Parasitology; 1961, 07, 3
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Wyniki hodowli tasiemców z rzędu Psudophyllidea in vitro
Conditions of Psudophyllidea tapeworm cultivation in vitro
Uslovija rosta cestod iz roda Psudophyllidea in vitro
Autorzy:: Machnicka-Roguska, B.
Powiązania:: https://bibliotekanauki.pl/articles/2179895.pdf
Data publikacji:: 1961
Wydawca:: Polskie Towarzystwo Parazytologiczne
Opis:: The review covers previous publications of Smyth dealing with cultivation of larval Cestode in vitro. Plecercoides were rearing in axenic conditions in vitro and obtained after 72 hours a mature from of Schistocephalus solidus. The plerocercoids were kept in a tube made of half permeable membrane which was placed in a vessel with fluent medium - horse serum; above vessel was heated to a temperature of 40° C being all the time shaked. The rearing of the coracides, out of Schistocephalus solidus ova obtained in vitro, procecded in water with a temperature or 25° C. The incubation of the coracides took place after 8 days. When the culture was trated with sun rays for several hours the process of the ova operculi opening was accelerated. The best results in cultivating plerocercoids Diphyllobothrium dendriticum in vitro up to a mature from was obtained on a medium containing an extract of duck embryo together with serum, monosacharides and albuminous hydrolysates. The plerocercoids after 6 days of breeding attained the stadium of late gametogenesis and shoved a defective somatic development. Any enrichment of the medium in nutritive substances failed to evoke further progress in the plerocercoids development - viz. formation of vitellous glands. In order to check whether the methods chosen for the development of tapeworms reared in vitro have been correct, cytologie, histological and histochemical tests have been performed. Cultivation in vitro is a contribution to the better understanding of relations and dependency existing between the parasite and its host.
Źródło:: Wiadomości Parazytologiczne; 1961, 07, 3; 561-566
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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