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Tytuł:
Evaluation of the effectiveness of currently used methods of identification of microorganisms in soils
Autorzy:
Kępa, Urszula
Stańczyk-Mazanek, Ewa
Powiązania:
https://bibliotekanauki.pl/chapters/2162963.pdf
Data publikacji:
2022-06-30
Wydawca:
Politechnika Częstochowska. Wydawnictwo Politechniki Częstochowskiej
Źródło:
Environmental safety of biowaste in the circural economy; 157-169
9788371938511
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The application of different methods for indirect microbial development assessment in pilot scale drinking water biofilters
Autorzy:
Holc, Dorota
Mądrecka-Witkowska, Beata
Komorowska-Kaufman, Małgorzata
Szeląg-Wasielewska, Elżbieta
Pruss, Alina
Cybulski, Zefiryn
Powiązania:
https://bibliotekanauki.pl/articles/2073779.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biofilm
drinking water treatment
pilot scale
microbiological activity
biological activated carbon filters
BAC
identification of microorganisms
Opis:
The biofiltration process in the biologically activated carbon filters (BAC) is one of advanced methods of water treatment. It enables efficient elimination of dissolved organic matter and some inorganic pollutants. The production of high-quality drinking water requires an appropriate method of filter work control based on biofilm growth assessment. The first aim of the study was to assess the microbial development in beds of two BAC filters with the use of various methods. The second aim was to compare the obtained results and indicate the method which could support filter operators during routine control of biofiltration process. The study was carried out in a pilot scale on models of BAC filters during two filter runs. The analysis of microorganisms was performed in water samples collected from different depths of the filter beds with the use of culture method (HPC), metabolic activity assay (with the FDA), epifluorescence microscopy – total cell count method (TCC) and biochemical method (system Vitek 2 Compact). No statistical correlation between HPC and metabolic activity assay was noted. Total bacteria number determined with the use of TCC was approx. 100–900 times higher than in the HPC method. The biochemical tests revealed the presence of several Gram-negative species. The comparison of the applied methods shows that microbial activity assay is the most useful, fast and low-cost method which may be applied additionally to the HPC method at standard water treatment plant laboratory.
Źródło:
Archives of Environmental Protection; 2021, 47, 3; 37--49
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of selected microorganisms from activated sludge capable of benzothiazole and benzotriazole transformation
Autorzy:
Kowalska, Katarzyna
Felis, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1038947.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biotransformation
benzothiazole
benzotriazole
DNA sequencing
PCR
Opis:
Benzothiazole (BT) and benzotriazole (BTA) are present in the environment - especially in urban and industrial areas, usually as anthropogenic micropollutants. BT and BTA have been found in the municipal and industrial wastewater, rivers, soil, groundwater, sediments and sludge. The origins of those substances' presence in the environment are various industry branches (food, chemical, metallurgical, electrical), households and surface runoff from industrial areas. Increasingly strict regulations on water quality and the fact that the discussed compounds are poorly biodegradable, make them a serious problem in the environment. Considering this, it is important to look for environmentally friendly and socially acceptable ways to remove BT and BTA. The aim of this study was to identify microorganisms capable of BT and BTA transformation or/and degradation in aquatic environment. Selected microorganisms were isolated from activated sludge. The identification of microorganisms capable of BT and BTA removal was possible using molecular biology techniques (PCR, DNA sequencing). Among isolated microorganisms of activated sludge are bacteria potentially capable of BT and BTA biotransformation and/or removal. The most common bacteria capable of BT and BTA transformation were Rhodococcus sp., Enterobacter sp., Arthrobacter sp. They can grow in a medium with BT and BTA as the only carbon source. Microorganisms previously adapted to the presence of the studied substances at a concentration of 10 mg/l, showed a greater rate of growth of colonies on media than microorganisms unconditioned to the presence of such compounds. Results of the biodegradation test suggest that BT was degraded to a greater extent than BTA, 98-100% and 11-19%, respectively.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 935-939
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efektywność usuwania związków organicznych podczas oczyszczania wody w procesie filtracji przez biologicznie aktywny filtr węglowy z identyfikacją mikroorganizmów
Effectiveness of Organic Compounds Removing During Water Treatment by Filtration Through a Biologically Active Carbon Filter with the Identification of Microorganisms
Autorzy:
Holc, D.
Pruss, A.
Michałkiewicz, M.
Cybulski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1818040.pdf
Data publikacji:
2016
Wydawca:
Politechnika Koszalińska. Wydawnictwo Uczelniane
Tematy:
filtry biologicznie aktywne
związki organiczne
identyfikacja mikroorganizmów
oczyszczanie wody
filtracja
biologically active filter (BAF)
organic compounds
identification of microorganisms
water treatment
filtration
Opis:
A study of effectiveness of organic compounds removing from the water was carried out in the pilot scale. Filter column with 100 mm diameter and 3 m height was filled with activated carbon WG-12 at the height of 2.1 m and placed in a water jacket. The water jacket was made with a pipe with the diameter of 140 mm, wherein water with the same temperature as filtered water, flows from top to bottom of jacket at all times. Activated carbon was biologically "inoculated" with backwash water taken from the carbon filters from existing Water Treatment Plant. Water samples were collected at the inlet and in the vertical profile of filter column. Following factors were analyzed in all samples: temperature, pH, dissolved oxygen, alkalinity, COD (KMnO4), UV254 absorbance, TOC, total number of mesophilic and psychrophilic bacteria. In some water samples, biochemical diagnostics were performed using an automated system Vitek 2 Compact (bioMerieux), in order to identify microorganisms. Samples of bed were also collected in the vertical profile of the filter to determine the total number of mesophilic and psychrophilic bacteria. Studies showed relatively short time of biological activation of filter bed, which undoubtedly was an effect of the proper preparation of the bed and conditions of the process (contact time, the optimum temperature and pH, and sufficient content of organic substances which was the nutrients for bacteria). Activated carbon WG-12, which was used during the studies, was a very good base for the growth of microorganisms in the filter bed. Microbial activity of filter was confirmed by indicator EMS which amounted to <1 and bacteriological analysis of water and the bed. The content of organic compounds in the water during filtration through a biologically active carbon bed decreased along to depth of filter. The lowering of organic compounds amount at higher depths of the filter bed was correlated with the growing amount of mesophilic and psychrophilic bacteria in the bed. In a vertical cross section of the filter Pseudomonas putida, Pseudomonas aeruginosa, Acinetobacter nosocomialis, Acinetobacter pittii, Acinetobacter baumannii, Acinetobacter calcoaceticus have been identified. Due to the fact that Pseudomonas putida and Pseudomonas aeruginosa are the bacteria responsible for the decomposition of organic compounds, their presence undoubtedly contributed to the reduction of biodegradable fraction of organic matter present in the filtered water.
Źródło:
Rocznik Ochrona Środowiska; 2016, Tom 18, cz. 2; 235-246
1506-218X
Pojawia się w:
Rocznik Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Removal of Organic Matter from Water During the Biofiltration Process – a Full Scale Technological Investigation
Usuwanie związków organicznych z wody w procesie biofiltracji – badania w skali technicznej
Autorzy:
Kołaski, Piotr
Wysocka, Agnieszka
Pruss, Alina
Lasocka-Gomuła, Iwona
Michałkiewicz, Michał
Cybulski, Zefiryn
Powiązania:
https://bibliotekanauki.pl/articles/1812068.pdf
Data publikacji:
2019
Wydawca:
Politechnika Koszalińska. Wydawnictwo Uczelniane
Tematy:
biologically active carbon filters
filtration
full scale technical investigation
identification of microorganisms
organic matter
water treatment
biologicznie aktywne filtry węglowe
badania w skali technicznej
filtracja
oczyszczanie wody
związki organiczne
identyfikacja mikroorganizmów
Opis:
The research was carried out on the full scale Water Treatment Plant with maximal capacity of 150 000 m3/d. Treated water is characterized by a high content of organic pollutants. In order to eliminate them from water and ensure the biological stability of water in the water supply network, in January 2015 a second stage of water treatment was launched, based on integrated ozonation and filtration through carbon filter beds. Between January and May 2016, samples of water and a filter bed were collected from four carbon filters and then physicochemical and bacteriological analysis were done. The FDA test and biochemical diagnostics were made to prove the microbiological activity of the filter bed. The studies showed a decrease in the content of organic compounds, meassured as TOC and COD (KMnO4), and the biological activity of the analyzed carbon filters. The carbon filter beds were populated by Pseudomonas fluorescens, Acinetobacter lwoffii, Aeromonas salmonicida and Sphingomonas paucimobilis. In none of the analyzed filters were found strains of the Enterobacteriaceae family which may have a potential threat to health of the consumers. The application of carbon filters has reduced the organic matter content in treated water.
Badania prowadzono w skali technicznej na Stacji Uzdatniania Wody (SUW) o maksymalnej wydajności 150 000 m3/d. Woda dopływająca do SUW charakteryzuje się zawartością specyficznych zanieczyszczeń organicznych. W celu ich eliminacji z wody oraz zapewnienia biologicznej stabilności wody w sieci wodociągowej, w styczniu 2015 r. uruchomiono drugi stopień oczyszczania wody, oparty o zintegrowane procesy ozonowania i filtracji przez złoże węglowe. Co miesiąc, w okresie od stycznia do maja 2016 r., pobierano próbki wody oraz złoża filtracyjnego z czterech filtrów węglowych. Próbki wody pobierano bezpośrednio znad złoża filtracyjnego oraz na odpływie z filtrów. Próbki złoża filtracyjnego pobierano z jego górnej warstwy, w pięciu punktach każdej komory filtracyjnej. Przeprowadzono analizy fizyczno-chemiczne i bakteriologiczne wody oraz złóż filtracyjnych. W celu wykazania aktywności mikrobiologicznej złóż wykonywano test aktywności esteraz z dwuoctanem fluoresceiny FDA. W próbkach wody i węgla aktywnego w celu zidentyfikowania mikroorganizmów prowadzono diagnostykę biochemiczną z wykorzystaniem zautomatyzowanego systemu Vitek 2 Compact (bioMerieux). Przeprowadzone badania wykazały obniżenie zawartości związków organicznych wyrażonych jako OWO i ChZT (KMnO4) oraz biologiczną aktywność analizowanych filtrów węglowych. Złoża filtrów węglowych zasiedlone były przez Pseudomonas fluorescens, Acinetobacter lwoffii, Aeromonas salmonicida oraz Sphingomonas paucimobilis. W żadnym z analizowanych filtrów nie wyhodowano natomiast szczepów z rodziny Enterobacteriaceae stanowiących potencjalne zagrożenie dla zdrowia konsumentów.Wprowadzenie filtrów węglowych do ciągu technologicznego SUW spowodowało obniżenie zawartości materii organicznej w wodzie uzdatnionej.
Źródło:
Rocznik Ochrona Środowiska; 2019, Tom 21, cz. 2; 1136-1155
1506-218X
Pojawia się w:
Rocznik Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and characterization of microbiological contamination sources in the environment of production of personalized femoral implant
Autorzy:
Gniada, Natalia
Leszczewicz, Martyna
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844919.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
implants
microbiology
microorganisms
implanty
mikrobiologia
mikroorganizmy
Źródło:
Engineering of Biomaterials; 2020, 23, 158 spec. iss.; 47
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of Bacteria from Drill Cuttings, their Identification and Assessment of Destructive Potential
Autorzy:
Chernykh, Elena G.
Powiązania:
https://bibliotekanauki.pl/articles/2086394.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
anionic surfactant
drill cuttings
carboxymethyl cellulose
diesel fuel
surfactant
cationic surfactant
oil-based drilling fluid
hydrocarbon-oxidizing microorganisms
Opis:
The drilling of any oil and gas well is accompanied by the use of drilling fluids consisting of chemical reagents and materials of varying degrees of environmental hazard. The existing drilling waste disposal technologies are expensive, energyand labor-intensive, and do not always contribute to the formation of environmentally friendly compounds. In this regard, the possibility of using destructor microorganisms capable of growing and exhibiting biochemical activity under the conditions of complex pollution, in an environment with a high content of hydrocarbons, surfactants and polymer additives capable of biodegrading these substances, is relevant. In connection with the foregoing, the search for new strains of bacteria for the disposal of drilling waste, which are resistant to the conditions of complex toxic effects of drill cuttings components and, at the same time, capable of providing a significant degree of detoxification of the reagents that are part of hydrocarbon-based solutions, is relevant.
Źródło:
Journal of Ecological Engineering; 2022, 23, 4; 155--163
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Otitis externa: the analysis of relationship between particular signs-symptoms and species and genera of identified microorganisms
Autorzy:
Kurnatowski, P.
Filipiak, J.
Powiązania:
https://bibliotekanauki.pl/articles/839086.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
external otitis
otitis
etiological factor
disease symptom
patient
laryngology
microorganism
identification
pathogen
bacteria
fungi
diagnosis
Źródło:
Annals of Parasitology; 2008, 54, 1; 37-41
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Otitis externa: the analysis of relationship between particular signs-symptoms and species and genera of identified microorganisms
Autorzy:
Kurnatowski, P.
Filipiak, J.
Powiązania:
https://bibliotekanauki.pl/articles/2143724.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
external otitis
otitis
etiological factor
disease symptom
patient
laryngology
microorganism
identification
pathogen
bacteria
fungi
diagnosis
Opis:
Objective. To investigate a relationship between the etiological factor of external otitis and occurrence of particular signs/symptoms. Design. A special questionnaire was designed and completed by all patients covering personal details, medical history, results of otolaryngological examination and bacteriological and mycological investigations. Subjects. 249 patients of the Outpatient Department of Laryngology at the Regional Hospital in Bełchatów with symptoms of external otitis. For analysis of relationships between particular signs/symptoms and species/genera of microorganisms, statistical tests were used: χ2 test, χ2 test with Yate’s modification, C−Pearson index, and Fisher exact test for very small samples. Results. There is a statistical dependence between discharge, hearing loss, swelling of skin, scant, dried discharge with fetid odour and bacteria isolated from the external ear canal. Similar dependence exists between pain, hearing loss, no smelly discharge or wet, black plug of fetid odour and fungi. Also there is a statistical dependence between pruritus, red skin and grey, fetid discharge and mixed flora. Conclusions. Some of symptoms and signs are connected with definite etiological factors which is important not only for correct diagnosis but also for institution of appropriate and effective treatment. On the basis of some characteristic symptoms and signs it is possible to make a tentative diagnosis as to the etiological pathogen responsible for external otitis.
Źródło:
Wiadomości Parazytologiczne; 2008, 54, 1; 37-41
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Probiotic Bacillus sp. environmental strains as a component of improved dishwasher cleaning product
Autorzy:
Banaszczyk, Justyna
Jędrzejczak, Gabriela
Zarzeczańska, Dorota
Ramotowska, Sandra
Fiutak, Marta
Skowron, Małgorzata
Ossowski, Tadeusz
Skowron, Piotr
Jeżewska-Frąckowiak, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1179658.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Dishwasher microbiota
MALDI-TOF microorganism identification
Probiotic microorganisms
Surface colonization
Opis:
The use of probiotics has become a mainstream application in pharmaceutical, food, cosmetic and cleaning technologies. Probiotic microorganisms seem to be a remedy for the problem of undesired, persistent microbiological contamination or uncontrolled biofilm formation in the automatic household equipments, dedicated to the undirect contact with food. We propose the mixture of commonly widespread and bio-safe environmental probiotic Bacillus sp. strains as a component of an improved cleaning product for automatic dishwashers. Our design of probiotic-improved cleaning product combines the chemical detergent formula and probiotic Bacillus sp. preparation in two separate pockets of self-soluble cleaning capsule. To pinpoint the validity of an improved probiotic-based cleaning product design, we present a case study of the microflora from the chosen localizations of an old household dishwasher. The swab collecting, followed by bacterial cultivation and MALDI-TOF identification revealed Enterobacter cloacae and Pseudomonas oleovorans forming the major inner microflora of an old household dishwasher, where a commonly available dishwashing products were used. We applied probiotic Bacillus sp. improved cleaning product to a model dishwasher in a conventional cleaning cycle and conducted the before-after trial to identify the inner microflora in chosen localizations. Using standard microbiological Gram-staining method with microscopic observation, followed by MALDI-TOF identification we confirmed, that Bacillus sp. replaced Acinetobacter sp., residing formerly model dishwasher filter and water drain. Conducted experiments have shown the potential for use of the chosen probiotic Bacillus sp. strains mixture as an addition for encapsulated formula of cleaning product, to provide microbiologically safe and controllable conditions during the conventional dishwasher cleaning cycle.
Źródło:
World Scientific News; 2017, 72; 141-149
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
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