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    Wyświetlanie 1-11 z 11
    Tytuł:
    Osteogenic differentiation of human mesenchymal stem cells from adipose tissue and Whartons jelly of the umbilical cord
    Autorzy:
    Zajdel, Alicja
    Kałucka, Magdalena
    Kokoszka-Mikołaj, Edyta
    Wilczok, Adam
    Powiązania:
    https://bibliotekanauki.pl/articles/1038664.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    adipose derived mesenchymal stem cells
    Wharton's jelly derived mesenchymal stem cells
    osteogenic differentiation
    Opis:
    Induced osteogenesis of mesenchymal stem cells (MSCs) may provide an important tool for bone injuries treatment. Human umbilical cord and adipose tissue are routinely discarded as clinical waste and may be used as noncontroversial MSCs sources. It still remains to be verified which source of MSCs is the most suitable for bone regeneration. The aim of this research was to investigate the osteogenic potential of human MSCs derived from adipose tissue (AT-MSCs) and Wharton's jelly of the human umbilical cord (WJ-MSCs) differentiated under the same conditions. Osteogenic differentiation of MSCs was detected and quantified by alizarin red S (ARS) staining for calcium deposition and alkaline phosphatase (ALP) activity, osteoprotegerin (OPG), and osteocalcin (OC) secretion measurements. Under osteogenic conditions, after 21 days of differentiation, the measured ALP activity and calcium deposition were significantly higher in the AT-MSCs than in the WJ-MSCs, while the OPG and OC secretion were higher in the WJ-MSCs vs. AT-MSCs. Low concentrations of OPG and high levels of OC in AT-MSCs and WJ-MSCs, prove that these cells reached an advanced stage of the osteogenic differentiation. The levels of OC secreted by AT-MSCs were lower than by WJ-MSCs. Both cell types, AT-MSCs and WJ-MSCs possess a potential to differentiate towards the osteogenic lineage. The observed differences in the levels of osteogenic markers suggest that after 21-days of osteogenic differentiation, the AT-MSCs might have reached a more advanced stage of differentiation than WJ-MSCs.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 2; 365-369
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Resorbable scaffolds modified with collagen type I or hydroxyapatite : in vitro studies on human mesenchymal stem cells
    Autorzy:
    Rumian, Ł.
    Wojak, I.
    Scharnweber, D.
    Pamuła, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/307304.pdf
    Data publikacji:
    2013
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    collagen type I
    human mesenchymal stem cells
    hydroxyapatite
    poly(L-lactide-co-glycolide)
    scaffolds
    kolagen
    hydroksyapatyt
    mezenchyma
    Opis:
    Poly(L-lactide-co-glycolide) (PLGA) scaffolds of pore size within the range of 250–320 μm were produced by solvent casting/ porogen leaching method. Afterwards, they were modified through adsorption of collagen type I and incubation in simulated body fluid (SBF) to allow deposition of hydroxyapatite (HAp). The wettability of the scaffolds was measured by sessile drop test. Scanning electron microscopy (SEM) evaluation and energy dispersive X-ray analysis (EDX) were also performed. SEM evaluation and EDX analysis depicted the presence of HAp deposits and a collagen layer on the pore walls on the surface and in the bulk of the scaffolds. Wettability and water droplets penetration time within the scaffolds decreased considerably after applying modifications. Human mesenchymal stem cells (hMSC) were cultured on the scaffolds for 28 days and cell morphology, proliferation and differentiation as well as calcium deposition were evaluated. Lactate dehydrogenase (LDH) activity results revealed that cells cultured on tissue culture polystyrene (TCPS) exhibited high proliferation capacity. Cell growth on the scaffolds was slower in comparison to TCPS and did not depend on modification applied. On the other hand, osteogenic differentiation of hMSC as confirmed by alkaline phosphatase (ALP) activity and mineralization results was enhanced on the scaffolds modified with hydroxyapatite and collagen.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2013, 15, 1; 61-67
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of in vitro cell culture conditions for human mesenchymal stem cells of different origin for applications in tissue engineering
    Autorzy:
    Kmiotek-Wasylewska, K.
    Karnas, E.
    Sekuła, M.
    Łabędź-Masłowska, A.
    Noga, S.
    Szkaradek, A.
    Dźwigońska, M.
    Boruczkowski, D.
    Madeja, Z.
    Zuba-Surma, E. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/284408.pdf
    Data publikacji:
    2018
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    cells
    tissue engineering
    regeneration
    Źródło:
    Engineering of Biomaterials; 2018, 21, 148; 25
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cell therapy for regenerative medicine: facts and controversy
    Fakty i kontrowersje związane z terapią komórkową w medycynie regeneracyjnej
    Autorzy:
    Sarnowska, Anna
    Machaliński, Bogusław
    Radoszkiewicz, Klaudia
    Bużańska, Leonora
    Powiązania:
    https://bibliotekanauki.pl/articles/2121315.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cell therapy
    regenerative medicine
    human mesenchymal stem cells
    human induced pluripotent stem cells
    Opis:
    Cellular therapy, as a part of regenerative medicine, implies to the treatment of human disorders with cells as a medical product, so called – “living drugs”. Usually such therapy is applied when other alternative efficient pharmacological therapies are not available. Stem cells of different origin: 1) tissue specific e.g. hematopethic, epithelial, neuronal, limbal; 2) mesenchymal stem cells (MSC) harvested from variety of tissues; 3) pluripotent stem cells: embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) – serve as a source of cells for regenerative medicine application, depending upon disease and application re- quirements. Currently MSC are the type of stem cells that are most frequently used in registered regenerative medicine clinical trials. In this paper we provide the information on the application of cell therapy in orthopedics, hematology, ophthalmology, dermatology, gastrology and neurology. The influence of origin of MSCs and iPSCs on their mode of action as therapeutic, regenerative agents are discussed. Advantages and disadvantages of application of different cell types for cell therapy are underlined. Last, but not least current low regulations in Poland and requirements of European regulatory bodies for cell therapy are pointed out and discussed.
    Źródło:
    Nauka; 2021, 4; 67-92
    1231-8515
    Pojawia się w:
    Nauka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged ferroelectric crystal surfaces
    Autorzy:
    Bacakova, L.
    Vandrovcova, M.
    Vanek, P.
    Petzelt, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/284786.pdf
    Data publikacji:
    2016
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    biomaterials
    mesenchymal stem cells
    crystal surfaces
    Źródło:
    Engineering of Biomaterials; 2016, 19, 138; 28
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Graphene-based substrates influence biological and functional properties of human umbilical cord-derived mesenchymal stem cells
    Autorzy:
    Sekuła, M.
    Karnas, E.
    Jagiełło, J.
    Noga, S.
    Adamczyk, E.
    Dżwigońska, M.
    Kmiotek, K.
    Baran, M.
    Madeja, Z.
    Lipińska, L.
    Zuba-Surma, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/284454.pdf
    Data publikacji:
    2016
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    mesenchymal stem cells
    cardiovascular diseases
    regeneration
    Źródło:
    Engineering of Biomaterials; 2016, 19, 138; 24
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged and uncharged ferroelectric crystal surfaces
    Autorzy:
    Vandrovcova, M.
    Bacakova, L.
    Vanek, P.
    Petzelt, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/285786.pdf
    Data publikacji:
    2016
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    electroactive ceramics
    surface charge
    cell number
    resazurin
    type I collagen
    alkaline phosphatase
    osteocalcin
    bone matrix mineralization
    Opis:
    The cell-material interaction is significantly influenced by the physicochemical properties of the material surface, including its electrical charge. In this study, the effect of the surface polarity of ferroelectric LiNbO3 single crystals on the adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells was investigated. The cells were cultured on the normal-to-plane poled and in-plane poled plates resulting in positive, negative and zero surface charge. The number of initially adhering cells on day 1 after seeding, their spreading, shape, and their metabolic activity, production of type I collagen, activity of alkaline phosphatase and mineralization in the following days of cultivation (days 6 and 20) were comparable on all three tested surfaces. However, significant differences were found in the expression of mRNA for type I collagen, alkaline phosphatase and osteocalcin, i.e. an early, medium-term and late arkers of osteogenic cell differentiation, respectively. On day 20, the expression of type I collagen was significantly lower in cells on negatively-charged than on non-charged surfaces. Moreover, the expression of alkaline phosphatase and osteocalcin was higher in cells on positively-charged than on negatively-charged surfaces. These differences were generally more pronounced in standard cell culture medium than in osteogenic medium, which could, at least partly, mask the influence of the material surface properties on the cell behaviour. Thus, positively-charged LiNbO3 surfaces seemed to be more suitable for the osteogenic differentiation of bone marrow mesenchymal stem cells than the negatively-charged surfaces.
    Źródło:
    Engineering of Biomaterials; 2016, 19, 135; 2-7
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    GMP compliant isolation and culture of human adipose tissue- derived mesenchymal stem cells for applications in tissue engineering
    Autorzy:
    Labedz-Maslowska, A.
    Szkaradek, A.
    Zuba-Surma, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/284325.pdf
    Data publikacji:
    2018
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    tissue engineering
    cells
    scaffolds
    Źródło:
    Engineering of Biomaterials; 2018, 21, 148; 71
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of innovative biopolymers designed for bone regeneration applications and in vitro study of their biocompatibility and osteogenic capacity on human bone marrow mesenchymal stem cells
    Autorzy:
    Basile, M. S.
    Gomez d'Ayala, G.
    Laurienzo, P.
    Malinconico, M.
    Oliva, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/284365.pdf
    Data publikacji:
    2012
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    functionalization of poly(ε-caprolactone)
    bone marrow mesenchymal stem cells
    Opis:
    In the frame of a project aiming to the improvement of the properties of biodegradable polyester-based devices, designed for bone regeneration, we have prepared and characterized novel composites based on poly(ε-caprolactone) (PCL). In particular, we report in this paper the functional changes made through radical grafting on PCL to obtain the less hydrophobic derivatives, PCL-MA-GMA [maleic anhydride (MA)-glycidylmethacrylate (GMA)] and PCL-DMAEA [N-(dimethyl-amino)ethylacrylate]. In addition, we studied in vitro the biocompatibility and the osteogenic capacity of these novel polyesters on human bone marrow (BM) mesenchymal stem cells (MSC).
    Źródło:
    Engineering of Biomaterials; 2012, 15, 113; 13-15
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The impact of unmodified graphene - based substrates on basic properties of human umbilical cord-derived mesenchymal stem cells in vitro
    Autorzy:
    Noga, S.
    Moździerz, A.
    Sekuła, M.
    Karnas, E.
    Jagiełło, J.
    Madeja, Z.
    Lipińska, L.
    Zuba-Surma, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/285808.pdf
    Data publikacji:
    2018
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    stem cells
    graphene
    substrates
    Źródło:
    Engineering of Biomaterials; 2018, 21, 148; 83
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of differentiation time of mesenchymal-stem-cell to tenocyte under a cyclic stretching with a microgrooved culture membrane and selected measurement cells
    Autorzy:
    Morita, Y.
    Yamashita, T.
    Toku, Y.
    Yu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/307018.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    różnicowanie
    kości
    stymulacja mechaniczna
    hBMSC
    cyclic stretch
    differentiation
    differentiation time
    human bone marrow-derived mesenchymal stem cell
    mechanical stimulus
    tenocyte
    Opis:
    There is a need for efficient stem cell-to-tenocyte differentiation techniques for tendon tissue engineering. More than 1 week is required for tenogenic differentiation with chemical stimuli, including co-culturing. Research has begun to examine the utility of mechanical stimuli, which reduces the differentiation time to several days. However, the precise length of time required to differentiate human bone marrow-derived mesenchymal stem cells (hBMSCs) into tenocytes has not been clarified. Understanding the precise time required is important for future tissue engineering projects. Therefore, in this study, a method was developed to more precisely determine the length of time required to differentiate hBMSCs into tenocytes with cyclic stretching stimulus. Methods: First, it had to be determined how stretching stimulation affected the cells. Microgrooved culture membranes were used to suppress cell orientation behavior. Then, only cells oriented parallel to the microgrooves were selected and evaluated for protein synthesis levels for differentiation. Results: The results revealed that growing cells on the microgrooved membrane and selecting optimally-oriented cells for measurement improved the accuracy of the differentiation evaluation, and that hBMSCs differentiated into tenocytes in approximately 10 h. Conclusions: The differentiation time corresponded to the time required for cellular cytoskeleton reorganization and cellular morphology alterations. This suggests that cells, when subjected to mechanical stimulus, secrete mRNAs and proteins for both cytoskeleton reorganization and differentiation.
    Źródło:
    Acta of Bioengineering and Biomechanics; 2018, 20, 1; 3-10
    1509-409X
    2450-6303
    Pojawia się w:
    Acta of Bioengineering and Biomechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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