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    Wyświetlanie 1-17 z 17
    Tytuł:
    Non-random base composition in codons of mitochondrial cytochrome b gene in vertebrates.
    Autorzy:
    Prusak, Beata
    Grzybowski, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1041499.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    structure-function relationships
    compositional bias
    cytochrome b
    Opis:
    Cytochrome b is the central catalytic subunit of the quinol:cytochrome c oxidoreductase of complex III of the mitochondrial oxidative phosphorylation system and is essential to the viability of most eukaryotic cells. Partial cytochrome b gene sequences of 14 species representing mammals, birds, reptiles and amphibians are presented here including some species typical for Poland. For the analysed species a comparative analysis of the natural variation in the gene was performed. This information has been used to discuss some aspects of gene sequence - protein function relationships. Review of relevant literature indicates that similar comparisons have been made only for basic mammalian species. Moreover, there is little information about the Polish-specific species. We observed that there is a strong non-random distribution of nucleotides in the cytochrome b sequence in all tested species with the highest differences at the third codon position. This is also the codon position of the strongest compositional bias. Some tested species, representing distant systematic groups, showed unique base composition differing from the others. The quail, frog, python and elk prefer C over A in the light DNA strand. Species belonging to the artiodactyls stand out from the remaining ones and contain fewer pyrimidines. The observed overall rate of amino acid identity is about 61%. The region covering Qo center as well as histidines 82 and 96 (heme ligands) are totally conserved in all tested species. Additionally, the applied method and the sequences can also be used for diagnostic species identification by veterinary and conservation agencies.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 4; 897-905
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Regulation of cytochrome b-561 protein family in maize (Zea mays L.) roots by iron and cadmium stress
    Autorzy:
    Ramanathan, K.
    Luthje, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/79976.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    maize
    Zea mays
    root
    abiotic stress
    cytochrome b561
    protein level
    real-time polymerase chain reaction
    maize root
    cadmium stress
    iron stress
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    On the mode of integration of the thylakoid membrane protein cytochrome b6 into cytoplasmic membrane of Escherichia coli
    Autorzy:
    Króliczewski, Jaroslaw
    Gubernator, Beata
    Rögner, Matthias
    Szczepaniak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1039882.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    membrane protein
    signal sequence
    integration/translocation
    cytochrome b6
    Opis:
    In the stroma compartment, several pathways are used for integration/translocation of chloroplast proteins into or across the thylakoid membrane. In this study we investigated the mode of incorporation of the chloroplast-encoded cytochrome b6 into the bacterial membrane. Cytochrome b6 naturally comprises of four transmembrane helices (A,B,C,D) and contains two b-type hemes. In the present study, mature cytochrome b6 or constructed deletion mutants of cytochrome were expressed in E. coli cells. The membrane insertion of cytochrome b6 in this bacterial model system requires an artificially added presequence that directs the protein to use an E. coli membrane-insertion pathway. This could be accomplished by fusion to maltose-binding protein (MBP) or to the bacterial Sec-dependent signal peptide (SSpelB). The integration of mature cytochrome b6 into the bacterial cytoplasmic membrane by the Sec pathway has been reported previously by our group (Kroliczewski et al., 2005, Biochemistry, 44: 7570). The results presented here show that cytochrome b6 devoid of the first helix A can be inserted into the membrane, as can the entire ABCD. On the other hand, the construct devoid of helices A and B is translocated through the membrane into the periplasm without any effective insertion. This suggests the importance of the membrane-anchoring sequences that are likely to be present in only the A and B part, and it is consistent with the results of computational prediction which did not identify any membrane-anchoring sequences for the C or D helices. We also show that the incorporation of hemes into the truncated form of cytochrome b6 is possible, as long as the B and D helices bearing axial ligands to heme are present.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 3; 335-343
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of two major cytochrome b lineages in pike-perch, Sander lucioperca, and first data on their distribution in European populations
    Autorzy:
    Kohlmann, K.
    Louati, M.
    Kersten, P.
    Bahri-Sfar, L.
    Poulet, N.
    Ben Hassine, O. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363206.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    cytochrome b
    PCR-RFLP
    pike perch
    Sander lucioperca
    cytochrom b
    sandacz
    Opis:
    Despite of the growing interest in pike-perch for aquaculture and its economic importance in fisheries, knowledge on the population structure and phylogeography of the species is still limited. We report the discovery of two major cytochrome b lineages and describe a simple method for their detection based on PCR amplification followed by restriction digestion with Alw26I. Screening of 708 individuals showed that haplotype A was fixed or dominating in Central and East European countries, whereas haplotype B was mainly found in several French populations and Tunisian pike-perch introduced from Europe. Sequencing the complete cytochrome b cds of 17 representative individuals revealed that haplotypes A and B differed by five substitutions but also showed further differentiation of both haplotypes due to an additional substitution in a single haplotype A and B individual from France, respectively. Five partial pike-perch cytochrome b cds available from NCBI GenBank could also clearly be assigned to one or the other of the two major lineages. Therefore, this new mtDNA marker might be considered as suitable not only for studies on population structure and phylogeography of pikeperch but also to trace its introduction history and to assess the genetic composition of aquaculture brood stocks.
    Źródło:
    Environmental Biotechnology; 2013, 9, 1; 1-5
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mössbauer Studies of Cu(II) Ions Interaction with the Non-Heme Iron and Cytochrome b$\text{}_{559}$ in a Chlamydomonas reinhardtii PSI Minus Mutant
    Autorzy:
    Burda, K.
    Kruk, J.
    Strzałka, K.
    Stanek, J.
    Schmid, G. H.
    Kruse, O.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044646.pdf
    Data publikacji:
    2006-03
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    82.39.Jn
    82.39.Rt
    82.50.Nd
    87.15.He
    87.64.Pj
    89.60.Fe
    Opis:
    Mössbauer spectroscopy was applied, for the first time, to study the interaction of copper ions with the non-heme iron and the heme iron of cytochrome b$\text{}_{559}$ in photosystem II thylakoids isolated from a Chlamydomonas reinhardtii photosystem I minus mutant. We showed that copper ions oxidize the heme iron and change its low spin state into a high spin state. This is probably due to deprotonation of the histidine coordinating the heme. We also found that copper preserves the non-heme iron in a low spin ferrous state, enhancing the covalence of iron bonds as compared to the untreated sample. We suggest that a disruption of hydrogen bonds stabilizing the quinone-iron complex by Cu$\text{}^{2+}$ is the mechanism responsible for a new arrangement of the binding site of the non-heme iron leading to its more "tense" structure. Such a diamagnetic state of the non-heme iron induced by copper results in a magnetic decoupling of iron from the primary quinone acceptor. These results indicate that Cu does not cause removal of the non-heme iron from its binding site. The observed Cu$\text{}^{2+}$ action on the non-heme iron and cytochrome b$\text{}_{559}$ is similar to that previously observed forα-tocopherol quinone.
    Źródło:
    Acta Physica Polonica A; 2006, 109, 3; 237-247
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of ancient mitochondrial DNA of the Baltic Sea sturgeon (Acipenser sp.)
    Autorzy:
    Fopp-Bayat, D.
    Ciesielski, S.
    Luczyński, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363174.pdf
    Data publikacji:
    2005
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    jesiotr
    historyczny mtDNA
    filogeneza molekularna
    gen cytochromu b
    Morze Bałtyckie
    Acipenser baeri
    Acipenser oxyrinchus
    Acipenser sturio
    ancient mtDNA
    cytochrome b gene
    molecular phylogeny
    Opis:
    Genetic relatedness between Baltic Sea sturgeon (Acipenser sturio L.) specimens caught in different geographic areas is not clear. According to previous studies, fish captured in different locations within the historic area of A. sturio habitation are genetically different to each other. We have examined a fragment (191 base pairs) of mitochondrial cytochrome b gene of four specimens of A. sturio found in Poland: three fish were preserved in museums of natural history and the bone of one fish was from an archaeological site. DNA sequences of the three museum samples were identical, whereas the DNA sequence of the archaeological sample differed in the 918 position of the cytochrome b gene. All the analyzed DNA fragments were similar to those of Acipenser baeri and genetically distant to Acipenser sturio and A. oxyrinchus.
    Źródło:
    Environmental Biotechnology; 2005, 1, 1; 29-33
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Paramecium tredecaurelia: A Unique Non-Polymorphic Species of the P. aurelia spp. Complex (Oligohymenophorea, Ciliophora)
    Autorzy:
    PRZYBOŚ, Ewa
    Tarcz, Sebastian
    Surmacz, Marta
    Sawka, Natalia
    Fokin, Sergei I.
    Powiązania:
    https://bibliotekanauki.pl/articles/763419.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    P. aurelia species complex, intraspecific polymorphism, rDNA (ITS1-5.8S- ITS2-5’LSU rDNA), cytochrome c oxidase subunit I gene (COI), cytochrome b gene (CytB)
    Opis:
    New stands of Paramecium tredecaurelia, a rare species of the P. aurelia spp. complex, were identified in Thailand and Madagascar on the basis of mating reactions and molecular markers (rDNA and mtDNA). Analysis of DNA fragments showed that all P. tredecaurelia strains, the recently recorded ones and the ones known previously from France, Mexico, and Israel, form a monophyletic and well-defined clade in the P. aurelia species trees. All of these strains, collected from different localities around the world, represent identical or nearly identical haplotypes in terms of all the studied DNA fragments. Given the huge distances between particular collection sites, such a low level of variability of the studied sequences may result from a slow rate of evolution in P. tredecaurelia.
    Źródło:
    Acta Protozoologica; 2013, 52, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of cytochrome CYP2B1/2 in nonpregnant, pregnant and fetal rats exposed to tobacco smoke.
    Autorzy:
    Czekaj, Piotr
    Wiaderkiewicz, Anna
    Florek, Ewa
    Wiaderkiewicz, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1044235.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    pregnancy
    CYP2B6
    rats
    cytochrome P450
    CYP2B1/2
    tobacco smoke
    Opis:
    Four-month-old female Wistar rats were exposed for 20 days to tobacco smoke obtained from non-filter cigarettes. During the exposure, concentration of tobacco smoke was monitored indirectly by measuring the CO level (1500 mg/m3 air). The efficacy of exposure was assessed by measuring urine nicotine and cotinine levels. Cigarette smoke did not change total cytochrome P450 and b5 protein levels in any of the organs studied, and most of these organs did not show any changes in the activity of reductases associated with these cytochromes. Following exposure to tobacco smoke, fetal rat liver expressed CYP2B1/2 protein; in newborns (day 1) both liver and lung showed CYP2B1/2 protein expression and very low pentoxyresorufin O-dealkylase activity. Western blot analysis of adult liver, lung, heart, but not of brain microsomes, showed that tobacco smoke induced CYP2B1/2 in both nonpregnant and pregnant rats, though its expression was lower in the livers and hearts of pregnant females. In the rat and human placenta, neither rat CYP2B1/2 nor human CYP2B6 showed basal or tobacco smoke-induced expression at the protein level. This study shows clearly that the expression of CYP2B1/2, which metabolizes nicotine and some drugs and activates carcinogens, is controlled in rats by age-, pregnancy-, and tissue-specific regulatory mechanisms.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 4; 1115-1127
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Overexpression of cytochrome P450 CYP6B7 mediated pyrethroid resistance in Indian strains of the cotton bollworm, Helicoverpa armigera (Hübner)
    Autorzy:
    Abd El-Latif, A.O.
    Kranthi, K.R.
    Kranthi, S.
    Sarwar, A.
    Subrahmanyam, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/65742.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The role of cytochrome P450 monooxygenase in pyrethroid resistance was studied in different strains of the cotton bollworm, Helicoverpa armigera, from India. Filed collected strains of Nagpur and Delhi were compared to the laboratory reared population. The results showed a high resistance to deltamethrin, α-cypermethrin, and β-cyfluthrin. The results also showed that this resistance could be reduced by using piperonyl butoxide (PBO). The Nagpur and Delhi strains were found to have a 2.40 and 1.79 fold higher monooxygenase activity compared to a susceptible strain. A strong, positive correlation between monooxygenase activity and pyrethroid resistance was observed (r = 0.86 - 0.98). The relative expression of the housekeeping gene, EF-1α, and three P450 genes, was studied in the 5th instar larval midgut of the three strains. Out of the three P450 genes examined, expression of CYP6B7 mRNA was not detected in the midgut of the susceptible strain though it was highly expressed in the resistant strains. The midgut of the Nagpur strain had a 2.60 fold overexpression of CYP6B7 mRNA compared to the moderately resistant, Delhi strain. The mRNA of CYP4G8 and CYP6B2 were not overexpressed in either the Nagpur or Delhi strain. The results indicated that the elevated cytochrome P450 monooxygenase activity is associated with pyrethroid resistance in Indian strains of H. armigera, and CYP6B7 could be the P450 form responsible for pyrethroid resistance.
    Źródło:
    Journal of Plant Protection Research; 2014, 54, 3
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phenobarbital-induced expression of cytochrome P450 genes.
    Autorzy:
    Czekaj, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1044233.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CYP2B
    cytochrome P450
    phenobarbital-responsive enhancer unit
    CYP2H1.
    orphan receptors
    CYP3A
    PXR
    CAR
    phenobarbital
    Opis:
    In contrast to the well-known Ah receptor-mediated regulation of the CYP1A1 gene by polycyclic aromatic hydrocarbons, the molecular mechanism by which phenobarbital (PB) and PB-like inducers affect transcription of CYP genes remains unknown; no receptor for these chemicals has been found to date. However, in the last 5 years PB-responsive sequences have been identified in the 5' flanking regions of several P450 genes. The phenobarbital-responsive enhancer unit (PBRU) of CYP2B gene family members contain two potential nuclear receptor binding sites (NR1 and NR2) that flank a nuclear factor 1 (NF-1) binding motif. The nuclear factors that regulate PBRU activity have not yet been characterized. It seems that PB may activate multiple nuclear orphan receptors to induce various CYP genes. CYP2B and CYP3A genes appear to be targets for the orphan receptors CAR and PXR, respectively. It is also possible that the pleiotropic effects of PB can, in part, be explained by the ability of the CAR-RXR heterodimer to bind to a variety of nuclear receptor binding motifs. The induction of cytochromes P450 may result in interactions between xenobiotics and in the interference of xenobiotic metabolism and endogenous signalling pathways.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 4; 1093-1105
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    THE EFFECT OF MULTIPLE-DOSE PAROXETINE ON THE PHARMACOKINETIC PROFILE OF CARVEDILOL IN RATS
    Autorzy:
    Abrudan, Maria B.
    Muntean, Dana Maria
    Neag, Maria A.
    Vlase, Laurian
    Gheldiu, Ana Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/895543.pdf
    Data publikacji:
    2018-10-31
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    cytochrome P-450
    pharmacokinetics
    paroxetine
    Carvedilol
    preclinical study
    Opis:
    The aim of the present study was to evaluate the effect of multiple-dose paroxetine upon the pharmacokinetic profile of carvedilol in rats. Carvedilol was orally administrated in rats (3.57 mg/kg body mass (b.m.)) in the absence of paroxetine or after a pre-treatment with multiple oral doses of paroxetine (7.14 mg/kg b.m.). The plasma concentrations of carvedilol were estimated by high performance liquid chromatography-tandem mass spectrometry. After carvediol co-administration with paroxetine, an approximately 4.5-fold increase in the exposure of carvedilol was observed, considering the significantly elevated value of AUC0-∞. Furthermore, an increase by 72% of peak plasma concentration was found, as well as an augmentation by 91% of the half life time of carvedilol was observed. Paroxetine co-administration led to a significant alteration of carvedilol’s pharmacokinetic profile in rats, these effects could be explained by the existence of a drug-drug interaction mediated by CYP2D6 inhibition.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 5; 1241-1246
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of alternative respiratory pathways in the yeast Schwanniomyces castellii by study of mutants deficient in cytochrome a+a_3 and/or b
    Autorzy:
    Clasisse, M.
    Boze, H.
    Dubreucq, E.
    Segueilha, L.
    Moulin, G.
    Galzy, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/1045696.pdf
    Data publikacji:
    1991
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1991, 38, 4; 365-392
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of Camellia sinensis extract on the expression level of transcription factors and cytochrome P450 genes coding phase I drug-metabolizing enzymes
    Wpływ ekstraktu z Camellia sinensis na poziom ekspresji czynników transkrypcyjnych i genów cytochromu P450 kodujących enzymy I fazy metabolizmu leków
    Autorzy:
    Bogacz, A.
    Karasiewicz, M.
    Bartkowiak-Wieczorek, J.
    Ozarowski, M.
    Seremak-Mrozikiewicz, A.
    Kujawski, R.
    Mikolajczak, P.L.
    Mrozikiewicz-Rakowska, B.
    Bobkiewicz-Kozlowska, T.
    Czerny, B.
    Grzeskowiak, E.
    Mrozikiewicz, P.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/71379.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    Camellia sinensis
    green tea
    plant extract
    expression level
    gene expression
    transcription factor
    cytochrome P450
    gene coding
    drug metabolism
    enzyme
    Opis:
    Green tea (Camellia sinensis) is widely used as a popular beverage and dietary supplement that can significantly reduce the risk of many diseases. Despite the widespread use of green tea, the data regarding the safety as well as herb-drug interactions are limited. Therefore, the aim of our study was to assess the influence of standardized green tea extract (GTE) containing 61% catechins and 0.1% caffeine on the expression level of rat CYP genes and the corresponding transcription factors expression by realtime PCR. The findings showed that GTE resulted in a significant decrease of CYP2C6 expression level by 68% (p<0.001). In case of CYP3A1 and CYP3A2, the mRNA levels were also reduced by extract but in a lesser degree compared to CYP2C6. Simultaneously the significant increase in the mRNA level of CAR, RXR and GR factors was observed by 54% (p<0.05), 79% (p<0.001) and 23% (p<0.05), respectively after 10 days of green tea extract administration. In addition, there was noted a small increase of CYP1A1 expression level by 21% (p>0.05) was noted. No statistically significant differences were observed for CYP1A2 and CYP2D1/2. In the same study we observed an increase in amount of ARNT gene transcript by 27% (p<0.05) in the long-term use. However, green tea extract showed the ability to stimulate HNF-1α both after 3 and 10 days of treatment by 30% (p<0.05) and 80% (p<0.001), respectively. In contrast, no change was observed in the concentration of HNF-4α cDNA. These results suggest that GTE may change the expression of CYP enzymes, especially CYP2C6 (homologue to human CYP2C9) and may participate in clinically significant interactions with drugs metabolized by these enzymes.
    Zielona herbata (Camellia sinensis) jest powszechnie stosowana jako napój i suplement diety i może istotnie zmniejszać ryzyko wystąpienia wielu chorób. Pomimo powszechnego 59 Effect of Camellia sinensis extract on the expression level of transcription factors and cytochrome P450 genes coding... Vol. 59 No. 4 2013 zastosowania zielonej herbaty, dane dotyczące bezpieczeństwa jak i interakcji preparatu roślinnego i leku syntetycznego są bardzo ograniczone. Celem badania była ocena wpływu standaryzowanego ekstraktu z zielonej herbaty (GTE) zawierającego 61% katechin i 0,1% kofeiny na poziom ekspresji szczurzych genów CYP i czynników transkrypcyjnych stosując technikę real-time PCR. Wyniki wykazały, że GTE znacznie obniża poziom ekspresji CYP2C6 o 68% (p<0,001). W przypadku CYP3A1 i CYP3A2 poziom mRNA tych genów był również redukowany przez ekstrakt, ale w mniejszym stopniu w porównaniu do CYP2C6. Istotny wzrost w poziomie mRNA obserwowano dla czynników CAR, RXR i GR odpowiednio o 54% (p<0,05), 79% (p<0,001) i 23% (p<0,05) po 10 dniach stosowania ekstraktu. Dodatkowo, zanotowano niewielki wzrost poziomu ekspresji CYP1A1 o 21% (p>0,05). Brak istotnych różnic zaobserwowano dla CYP1A2 i CYP2D1/2. W badaniu wykazano również wzrost ilości transkryptu genu ARNT o 27% (p<0,05) podczas dłuższego stosowania. Ponadto, ekstrakt z zielonej herbaty wykazał zdolność do stymulacji HNF-1α zarówno po 3, jak i 10 dniach trwania eksperymentu odpowiednio o 30% (p<0,05) i 80% (p<0,001). Brak zmian obserwowano w przypadku stężenia cDNA dla HNF-4α. Wyniki te sugerują, że GTE może zmieniać ekspresję enzymów CYP, szczególnie w przypadku CYP2C6 (homolog ludzki CYP2C9) i może uczestniczyć w klinicznie istotnych reakcjach z lekami metabolizowanymi przez te enzymy.
    Źródło:
    Herba Polonica; 2013, 59, 4
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A primary attempt of Leptinotarsa decemlineata control using contact DNA insecticide based on short antisense oligonucleotide of its CYP6B gene
    Autorzy:
    Oberemok, V.
    Laikova, K.
    Shumskykh, M.
    Kenyo, I.
    Kasich, I.
    Deri, K.
    Seidosmanova, E.
    Krasnodubets, A.
    Bekirova, V.
    Gal'chinsky, N.
    Powiązania:
    https://bibliotekanauki.pl/articles/66808.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Leptinotarsa decemlineata
    pest control
    DNA insecticide
    insecticide
    antisense oligonucleotide
    cytochrome P450
    CYP6B gene
    potato
    Opis:
    Effective control of Leptinotarsa decemlineata remains an urgent problem for agriculture worldwide. Minimization of the use of non-selective neonicotinoid insecticides, such as thiomethoxam, is an actual vector of development of potato cultivation. In this rapid communication, we show the prospect of the topical use of short unmodified antisense fragment of L. decemlineata CYP6B gene as a DNA insecticide. Investigated parameters, namely, number of larvae per plant, aboveground biomass, yield and number of potatoes produced per plant indicate the possibility of this post-genomic approach as a safe and effective method of L. decemlineata control.
    Źródło:
    Journal of Plant Protection Research; 2018, 58, 1
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of Panax ginseng and Ginkgo biloba extracts on expression level of transcriptional factors and xenobiotic-metabolizing cytochrome P450 enzymes
    Wpływ ekstraktów z Panax ginseng i Ginkgo biloba na poziom ekspresji czynników transkrypcyjnych i enzymów cytochromu P450 metabolizujących ksenobiotyki
    Autorzy:
    Bogacz, A.
    Karasiewicz, M.
    Dziekan, K.
    Procyk, D.
    Gorska-Paukszta, M.
    Kowalska, A.
    Mikolajczak, P.L.
    Ozarowski, M.
    Czerny, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/71862.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Opis:
    Introduction: Despite widespread use of Panax ginseng and Ginkgo biloba, the data on the safety as well as herb-drug interactions are very limited. Therefore, we postulate that P. ginseng and G. biloba may modulate the activity and content of cytochrome P450 isozymes involved in the biotransformation of diverse xenobiotic substances. Objective: The aim of our study was to determine the influence of herbal remedies on the expression level of CYP enzymes and transcriptional factors. Methods: Male Wistar rats were given standardized Panax ginseng (30 mg/kg p.o.) or standardized Ginkgo biloba (200 mg/kg p.o.) for 3 and 10 days. The expression in liver tissue was analyzed by realtime PCR method. Results: Our results showed a decrease of CYP3A1 (homologue to human CYP3A4) mRNA level after P. ginseng extract treatment. The CYP2C6 (homologue to human CYP2C9) expression was also reduced. Additionally, after 10 days of the treatment with P. ginseng an increase of CYP1A1 (homologue to human CYP1A1) and CYP1A2 (homologue to human CYP1A2) expression was observed. Moreover, G. biloba extract also caused an increase of expression level for CYP1A1, CYP2C6, CYP3A1 and CYP3A2. Conclusion: Our findings suggest that herbal extracts can modulate the expression of transcriptional factors and CYP enzymes involved in xenobiotic metabolism and chemical carcinogenesis.
    Wstęp: Mimo powszechnego stosowania Panax ginseng i Ginkgo biloba dane dotyczące bezpieczeństwa, a także interakcji pomiędzy preparatami roślinnymi a lekami syntetycznymi są bardzo ograniczone. W niniejszych badaniach założono, iż żeń-szeń oraz miłorząb mogą modulować aktywność i zawartość izoenzymów cytochromu P450 biorących udział w biotransformacji różnych substancji ksenobiotycznych. Cel: Określenie wpływu preparatów roślinnych na poziom ekspresji enzymów CYP i ich czynników transkrypcyjnych. Metody: Szczurom rasy Wistar podawano standaryzowany Panax ginseng (30 mg/kg) oraz Ginkgo biloba (200 mg/kg) przez 3 do 10 dni. Ekspresję w tkance wątrobowej analizowano za pomocą metody PCR w czasie rzeczywistym. Wyniki: Uzyskane wyniki wykazały spadek poziomu mRNA CYP3A1 (homolog ludzkiego enzymu CYP3A4) po podaniu ekstraktu z żeń-szenia. Ekspresja genu CYP2C6 (homolog ludzkiego enzymu CYP2C9) również uległa obniżeniu. Dodatkowo, obserwowaliśmy wzrost ekspresji CYP1A1 (homolog ludzkiego enzymu CYP1A1) i CYP1A2 (homolog ludzkiego enzymu CYP1A2) po 10 dniach stosowania P. ginseng. Ponadto, ekstrakt z G. biloba spowodował również wzrost poziomu mRNA CYP1A1, CYP2C6, CYP3A1 i CYP3A2 w modelu in vivo. Wnioski: Badania sugerują, że wyciągi roślinne mogą modulować ekspresję czynników transkrypcyjnych i enzymów CYP uczestniczących w metabolizmie ksenobiotyków i chemicznej karcynogenezie.
    Źródło:
    Herba Polonica; 2016, 62, 1
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular mechanisms of regulation of CYP enzymes of phase I metabolism of xenobiotics - synthetic drugs and herbal preparations
    Molekularne mechanizmy regulacji enzymów CYP i fazy metabolizmu ksenobiotyków - leków sysntetycznych i preparatów roślinnych
    Autorzy:
    Bogacz, A.
    Karasiewicz, M.
    Kujawski, R.
    Bartkowiak-Wieczorek, J.
    Cichocka, J.
    Kowalska, A.
    Czerny, B.
    Rudzinska, K.
    Grzeskowiak, E.
    Mrozikiewicz, P.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/72700.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    molecular mechanism
    regulation mechanism
    cytochrome P450
    metabolism phase
    xenobiotic
    synthetic drug
    herbal preparation
    herbal product
    Opis:
    The understanding of the pharmacokinetic properties of synthetic drugs as well as herbal preparations used in treatment of many diseases offers a real opportunity to eliminate or minimize the interaction between the active substances. This is of particular clinical importance because it can decrease or increase the action of drugs what is the major cause of failure of pharmacotherapy. Understanding the molecular basis of interaction of substances with enzymes of drug metabolism is extremely helpful in treatment planning and its practical application. The experimental and clinical studies aimed to complete the characterization of the mechanisms of many factors that modulate the activity of cytochrome P450 enzymes, can provide a lot of evidence guaranteeing the safety of herbal products.
    Zrozumienie właściwości farmakokinetycznych leków syntetycznych, jak również preparatów roślinnych stosowanych w leczeniu wielu schorzeń stwarza realną szansę na eliminację lub zminimalizowanie interakcji pomiędzy substancjami aktywnymi. Ma to szczególne znaczenie kliniczne, ponieważ może prowadzić do osłabienia bądź nasilenia działania leków znajdujących się u podłoża niepowodzeń farmakoterapii. Poznanie podłoża molekularnego interakcji substancji z enzymami metabolizmu leków jest niezwykle pomocne podczas planowania terapii i jej praktycznego zastosowania. Wiele dowodów gwarantujących bezpieczeństwo stosowania produktów zielarskich mogą dostarczyć badania eksperymentalne oraz kliniczne zmierzające do pełnej charakterystyki mechanizmów działania wielu czynników modulujących aktywność enzymów cytochromu P450.
    Źródło:
    Herba Polonica; 2012, 58, 3
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of Epilobium angustifolium and Serenoa repens extracts on cytochrome 2D2 and 3A1 expression level in rats
    Wpływ ekstraktów z Epilobium angustifolium i Serenoa repens na poziom ekspresji cytohromów 2D2 i 3A1 w modelu szczurzym
    Autorzy:
    Kujawski, R.
    Mrozikiewicz, P.M.
    Mikolajczak, P.L.
    Kuzio, G.
    Bogacz, A
    Cichocka, J.
    Bartkowiak-Wieczorek, J.
    Grzeskowiak, E.
    Uzar, I.
    Bobkiewicz-Kozlowska, T.
    Czerny, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/72804.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Opis:
    Benign prostatic hyperplasia (BPH) is a common disease affecting aging males. In recent years, as alternative strategy for the prevention and therapy of BPH, there is an growing interest in usage of plant derived remedies i.e. from Serenoa repens (sabal palm) and some plants from the Epilobium genus. The aim of this study was to investigate the influence of standardized Epilobium angustifolium L. and Serenoa repens extracts on expression level of CYP3A1 and CYP2D2 mRNAs in rats. Testosterone and standardized Epilobium angustifolium or commercial Serenoa repens extracts were given for 21 days to castrated male Wistar rats. The levels of CYP2D2 and CYP3A1 mRNAs expression were analyzed by real-time quantitative PCR using specific target primers. We have observed a slightly increased level of CYP2D2 mRNA in animals treated with testosterone and both plant extracts (by 4.6% in rats receiving Epilobium angustifolium extract and by 25.29% in animals treated with extract from Serenoa repens) and the CYP3A1 mRNA level by 11.02% in rats treated with Serenoa repens extract. In rats receiving E. angustifolium extract a 20.22% decrease of CYP3A1 mRNA expression level was observed. Results from our work showed that standardized plant extracts from Epilobium angustifolium and Serenoa repens have differentially influenced on CYP3A1 and CYP2D2 mRNA expression level in a rat liver.
    Łagodny rozrost gruczołu krokowego jest powszechnym schorzeniem występującym u starzejących się mężczyzn. W ostatnich latach wzrosło zainteresowanie roślinami leczniczymi należącymi do gatunku boczni piłkowanej (Serenoa repens) i rodzaju wierzbownica (Epilobium sp.), które mogą stać się alternatywną strategią profilaktyki i leczenia BPH. Celem pracy było ustalenie wpływu standaryzowanych wyciągów z Epilobium angustifolium i Serenoa repens na ekspresję cytochromów CYP3A1 i 2D2 u szczurów. Kastrowanym samcom szczurów rasy Wistar podawano przez okres 21 dni testosteron i badane, standaryzowane wyciągi roślinne. Zmiany poziomu ekspresji mRNA CYP3A1 i 2D2 mierzono techniką PCR w czasie rzeczywistym z użyciem starterów specyficznych dla cytochromów. Stwierdzono nieznaczne podwyższenie poziomu ekspresji mRNA CYP2D2 u zwierząt otrzymujących testosteron i oba wyciągi roślinne (podwyższenie o 4,6% u szczurów otrzymujących wyciąg z Epilobium angustifolium i o 25,29% u zwierząt otrzymujących wyciąg z Serenoa repens) i wzrost poziomu ekspresji mRNA CYP3A1 o 11,02% u zwierząt otrzymujących wyciąg z Serenoa repens. U szczurów otrzymujących wyciąg z Epilobium angustifolium stwierdzono obniżenie poziomu ekspresji mRNA CYP3A1 o 20,22%. Wyniki naszej pracy wykazały, że standaryzowane wyciągi z Epilobium angustifolium i Serenoa repens w zróżnicowany sposób wpływają na poziom transkrypcji CYP3A1 i CYP2D2 mRNA w wątrobie szczura.
    Źródło:
    Herba Polonica; 2010, 56, 4
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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