- Tytuł:
- 1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5
- Autorzy:
-
Dąbrowski, Sławomir
Pietrewicz-Kubicz, Dorota
Zabłotna, Ewa
Długołęcka, Anna - Powiązania:
- https://bibliotekanauki.pl/articles/1039710.pdf
- Data publikacji:
- 2012
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
anaerobic fermentation
Clostridium butyricum
Escherichia coli
biodiesel
dha operon
glycerol
1,3-propanediol - Opis:
- 1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
- Źródło:
-
Acta Biochimica Polonica; 2012, 59, 3; 357-361
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki