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Tytuł:
The use of infrared spectroscopy and artificial neural networks for detection of uropathogenic Escherichia coli strains susceptibility to cephalothin
Autorzy:
Lechowicz, Łukasz
Urbaniak, Mariusz
Adamus-Białek, Wioletta
Kaca, Wiesław
Powiązania:
https://bibliotekanauki.pl/articles/1039472.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
infrared spectroscopy
artificial neural network
uropathogenic Escherichia coli
antibiotic resistance
Opis:
Background & Aims: Infrared spectroscopy is an increasingly common method for bacterial strains' testing. For the analysis of bacterial IR spectra, advanced mathematical methods such as artificial neural networks must be used. The combination of these two methods has been used previously to analyze taxonomic affiliation of bacteria. The aim of this study was the classification of Escherichia coli strains in terms of susceptibility/resistance to cephalothin on the basis of their infrared spectra. The infrared spectra of 109 uropathogenic E. coli strains were measured. These data are used for classification of E. coli strains by using designed artificial neural networks. Results: The most efficient artificial neural networks classify the E. coli sensitive/resistant strains with an error of 5%. Conclusions: Bacteria can be classified in terms of their antibiotic susceptibility by using infrared spectroscopy and artificial neural networks.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 713-718
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Susceptibility to antibiotics of bacterial microorganisms most commonly isolated from clinical and subclinical forms of mastitis in cows
Autorzy:
Radzikowski, Daniel
Powiązania:
https://bibliotekanauki.pl/articles/1178235.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
antibiotic therapy
antibiotics
dairy cows
mastitis
resistance
sensitivity
Opis:
Mastitis is an increasing problem in dairy farms and in the dairy industry. The paper presents changes in susceptibility of strains of bacteria isolated from quarter milk cows from mastitis in 2011, 2012, 2013, 2014, 2015 in the Factory Laboratory of Dairy Cooperative in south-eastern Poland. In total, 3409 strains of staphylococci, 2774 strains of streptococci and 423 strains of E. coli were isolated in these years. Susceptibility to antibiotics was examined by the disc diffusion method on Mueller-Hinton base. On the basis of the research it can be concluded that the highest sensitivity of staphylococci in all study periods is found for cefguin (from 91.2% to 96.1%) and cefapirin (92.2% to 96.2%), and the smallest for tetracycline (18 %), streptomycin (11.9%) and amoxicillin (9.3%). The highest sensitivity of bacteria from the Streptococcus family are cefapirin (97%), cefquinome (96.1%), amoxycycline (95.6%) and cefalexin (89.9%) on average in all years of research. The highest resistance was found for neomycin and streptomycin. They show that during the period under study over 90% of Escherichia coli were sensitive to streptomycin. Sensitivity at the level of 70%-80% was noted for amoxicillin, neomycin and cefapirin. The highest resistance of E. coli bacilli evolved for cefallaxin and tetracycline. Accordingly, early diagnosis of a pathogenic pathogen, and then selecting the appropriate form of treatment, gives you the opportunity to minimize the loss of mastitis in dairy cows.
Źródło:
World Scientific News; 2018, 93; 107-114
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Środowisko a antybiotykooporność izolatów Escherichia coli
Envirnomental dissemision of resistance among Escherichia coli isolates
Autorzy:
Krzyśko-Łupicka, T.
Kręcidło, M.
Mysłek, M.
Kręcidło, Ł.
Powiązania:
https://bibliotekanauki.pl/articles/126404.pdf
Data publikacji:
2017
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
metoda Kirby-Bauera
testowanie antybiotykoodporności
Escherichia coli
Kirby-Bauera method
antibiotic susceptibility testing
Opis:
Pula farmaceutyków w środowisku powiększa się w wyniku kontaminacji wód, gruntów, ścieków, a także surowców pochodzenia zwierzęcego. Obecność antybiotyków w środowisku wpływa nie tylko na zahamowanie rozwoju mikroorganizmów, ale w stężeniu subinhibicyjnym może stymulować ekspresję genów wywołujących zjawisko antybiotykooporności. Celem pracy było porównanie wrażliwości na antybiotyki pałeczek Escherichia coli wyizolowanych z surowego mleka i zmieszanych odpadów drobiarskich. Materiał badawczy stanowiły szczepy E.coli wyizolowane z surowca mleczarskiego i zmieszanych odpadów drobiarskich. Ocenę wrażliwości izolatów na antybiotyki przeprowadzono metodą Kirby-Bauera. Profil oporności E. coli ustalano na podstawie rekomendacji CLSI. Wykorzystano krążki firmy BTL wysycone takimi antybiotykami, jak: ampicylina (AM10), chloramfenikol (C30), gentamycyna (CN10), tetracyklina (TE30) oraz mieszaniną sulfmetoksazolu i trimetoprimu (SXT25). Izolaty E. coli z surowca mleczarskiego charakteryzowały się większą opornością na ampicylinę i wyższą wrażliwością na pozostałe badane antybiotyki w porównaniu do izolatów z odpadów drobiarskich.
Recently amount of pharmaceutical substances in the environment is getting higher due to spreading and relocation of water and soil contamination by sources such as a livestock production or sewage. The persistence of antibiotic in the environment may affect both the inhibition of microbial growth and also the stimulation of resistance gens expression, when antibiotics are in the subinhibitory concentration. The purpose of a study was to compare susceptibility of Escherichia coli isolates from two following sources: a raw milk from dairy and a poultry waste. The assessment of susceptibility on antibiotic was carried out by the Kirby-Bauer’s method. The resistance profile of isolates was estimated with using of CLSI standards. Antibiotic discs from BTL company were tested with particular type of substances: ampicillin (AM10), chloramphenicol (C30), gentamicin (CN10), tetracycline (TE30) and solution of sulfamethoxazole and trimethoprim (SXT25). Isolates of E. coli from raw milk were more resistant to ampicillin and characterised by higher susceptibility to the other antibiotics in the comparison to the isolates from poultry waste.
Źródło:
Proceedings of ECOpole; 2017, 11, 2; 517-523
1898-617X
2084-4557
Pojawia się w:
Proceedings of ECOpole
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Skin and nasal vestibule colonisation by Staphylococcus aureus and its susceptibility to drugs in atopic dermatitis patients
Autorzy:
Wrobel, J.
Tomczak, H.
Janerowicz, D.
Czarnecka-Operacz, M.
Powiązania:
https://bibliotekanauki.pl/articles/2081660.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
microbiota
antibiotic resistance
atopy
skin barrier
eczema
superinfection
colonisation
Opis:
Introduction. Atopic dermatitis (AD) is a chronic and recurrent disease with an inflammatory background. Genetic and environmental factors are responsible for the occurrence and development of this dermatosis. Objective. The aim of the study was to assess the frequency of colonisation of the nasal vestibule and apparently healthy skin by S. aureus, and to assess dermal lesions for superinfection with S. aureus in AD patients. Materials and method. The research was performed on a population of 100 AD patients. Three smears were collected for microbiological investigations: from the anterior nares, from apparently healthy skin and from lesioned skin. On collection, the material was cultured on mediums provided by the bioMerieux and Argenta companies. The strains were identified and their sensitivity to antibiotics assessed by means of the Vitek2 system (bioMerieux). Results. S. aureus was present in at least one location in 90%. 44% presented S. aureus colonization of apparently healthy skin and within skin lesions. In the case of 23%, this particular pathogen was detected only in skin lesions, whereas 6% had a positive result within the area of apparently healthy skin. In total, S. aureus was detected in the skin of 73%. In 61%, apparently healthy skin had been colonized with S. aureus, whereas in 81% colonization was proved within the skin lesions. S. aureus was detected in the nasal vestibule of 85%. Among the group with S. aureus in their anterior nares, the pathogen was also found on the skin surface of 77%. Conclusions. S. aureus colonises skin in most AD patients. Most AD patients carry S. aureus in their nasal vestibules. S. aureus does not seem to colonise healthy skin as often as skin lesions in young people. The vast majority of S. aureus strains were sensitive to local antibiotics. Most S. aureus strains produced penicillinase.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 334-337
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence and antimicrobial susceptibility of Klebsiella pneumoniae isolated from hospitalized patients at General Hospital, Etim Ekpo, Akwa Ibom State, Nigeria
Autorzy:
Anosike, I. Kelechi
Edet, U. Okon
Umoafia, G. E.
Agbo, B. E.
Ejelonu, V. O.
Onyesoro, V.
Powiązania:
https://bibliotekanauki.pl/articles/1030832.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibiotic susceptibility
Klebsiella pneumonia
Nosocomial
Prevalence
Resistance
Opis:
Klebsiella pneumoniae (KP) was investigated in respondents presenting signs and symptoms of urinary tract infection (UTI) in a rural community. Using simple random sampling, a total of 360 respondents (144 males and 216 females) were recruited into the study following informed consent and ethical approval. Mid-stream urine samples were collected from all the respondents aseptically using standard protocol. Isolation of K. pneumoniae was done using morphological characteristics and various biochemical tests while antibiotic sensitivity testing using various antibiotics was done using guidelines of Clinical and Laboratory Standard Institute. A total of 16 samples gave positive culture representing an overall prevalence of 4.4%. Furthermore, prevalence rates of 2.8%, 5.6%, 47.82%, 4.9%, 3.50%, and 8.60% for males, females, diabetics, in-patients, out-patients, and respondents above 60 years of age respectively were obtained. Resistance to antibiotics ranged from 37.50 to 56.25% for gentamycin and nalidixic, and tarivid, respectively. There is need for preventive measures aimed at sensitization of dwellers in community settings.
Źródło:
World Scientific News; 2020, 145; 222-233
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence and Antibiotic Susceptibility Patterns of Shigella and Salmonella Causing Diarrhoea in Children Below 5 Years at Thika Level Five District Hospital
Autorzy:
Kibet, S. C.
Matiru, V. N.
Kibaba, P. W.
Mucheke, A.
Louis, H.
Powiązania:
https://bibliotekanauki.pl/articles/1114108.pdf
Data publikacji:
2017
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibacterial
Children
Diarhoea
Salmonella
Shigella
Opis:
The aim of the study was to isolate and identify the extent of Salmonella and Shigella induced diarrhoea in children under five and to determine their antibacterial susceptibility patterns. The method of investigation was a cross-sectional study. Samples were collected from children less than five years old afflicted with diarrhoea coming for treatment at Thika Level Five Hospital, Kiambu County. A total of 80 stool samples were collected. These were specifically examined for Shigella and Salmonella. In doing so, the samples were cultured in MacConkey and Xylose lactose deoxycholate (XLD) agar. The plates were subsequently incubated aerobically at 37 °C overnight. After incubation, suspected Salmonella and Shigella colonies were identified morphologically, marked and the colonies inoculated to biochemical tests for species identification as described in the Medical Laboratory Manual. Following this, colonies derived from purity plating through biochemical testing were sub-cultured onto nutrient agar to obtain pure colonies. The obtained pure colonies were then used to perform susceptibility tests to commonly prescribed antibacterial drugs, including Gentamicin, Ciproflaxicin and Erythromycin. Results showed 10 (12.5%) confirmed positive isolates, where 6 (60%) were Salmonella and 4 (40%) Shigella. With respect to age, both isolates were found to be concentrated more at ages of 1-4 years. Herein, Salmonella – 1-2 years (33.33%), - 2-3years (50%); Shigella – 1-2 years (50%), - 2-3 years (25%). From the study, sex distribution (male or female) of the host isolates was equal (50-50%). Moreover, Ciprofloxacin and Gentamycin were the most potent antibiotics, whereas Ampicillin, Erythromycin, Cotrimoxazole and Sulfamethoxazole were highly resisted. According to study results, Quinolones and Aminogylcosides are the antibiotics of choice for severe diarrhoea illnesses caused by Salmonella and Shigella.
Źródło:
World News of Natural Sciences; 2017, 11; 28-36
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasmid profile analysis and evaluation of antibiotic susceptibility of Staphylococcus aureus strains isolated from table chicken eggs
Autorzy:
Pyzik, E.
Marek, A.
Powiązania:
https://bibliotekanauki.pl/articles/31431.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to isolate and characterize Staphylococcus aureus bacteria present on the shell surfaces and in the contents of chicken eggs, taking into account their phenotypic properties, antibiotic susceptibility patterns, and the presence of plasmid DNA. The study included 90 table chicken eggs from laying farms situated in the vicinity of Lublin. A total of 105 bacterial strains identified as Staphylococcus were isolated from the material, of which 18 (17.14%) were of the species Staphylococcus aureus. All 18 S. aureus strains were found to be resistant to at least one of the antibiotics tested, while some (55.55%) showed resistance to five or more of the 17 therapeutic agents. The greatest number of strains showed resistance to erythromycin (66.66%), tetracycline (66.66%), oxytetracycline (61.11%), penicillin G (50%), and amoxicillin (44.44%). The plasmid profile analysis of the S. aureus strains made it possible to evaluate the dependence between antibiotic susceptibility and the presence of plasmids in particular isolates. The results showed that plasmids in various quantities and of varying molecular weights were isolated from 17 of the strains. Most often isolated were small plasmids, of 5.6 kb – from 11 of the S. aureus strains (61.11%), 2.5 kb – from 9 strains (50%), 4.1 kb – from 8 (44.44%), and 4.6 kb – from 7 (38.88%) of the strains.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Decreased antibiotic susceptibility and enhanced probiotic production potential of goat milk fermented curd in comparison with cow and buffalo milk
Autorzy:
Lakhanpal, J.
Gupta, J.
Powiązania:
https://bibliotekanauki.pl/articles/80240.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
probiotic
goat milk
buffalo
milk
cow milk
curd cheese
antibiotic resistance
organoleptic characteristics
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of class 1, 2, 3 integrons, ESBL genes and antibiotic susceptibility of Salmonella serotypes from broiler and cattles in Turkey
Autorzy:
Sahan Yapicier, O.
Ozturk, D.
Powiązania:
https://bibliotekanauki.pl/articles/16539245.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
antibiotic susceptibility
broiler
cattle
integrons
β-lactamase
MARI
Salmonella
tet genes
Opis:
Antimicrobial resistance in Salmonella has been associated with the presence of integrons and many other resistance mechanisms contributing to the spread of antimicrobial-resistant genes within and between livestock and human populations. In this study, the presence of Salmonella serovars from broiler and cattle samples and their antimicrobial resistance, integrons, tet resistance, ESBL and resistance genes carriage were investigated. Total of 209 litter (broiler farms) and fecal samples (cattle farms) were examined by bacteriological procedures, susceptibilities against 18 antimicrobials and genes carriages were detected by singleplex and multiplex PCR. A total of 46/209 (22 %) Salmonella strains were isolated. Six different Salmonella serotypes from 46 Salmonella isolates were identified and the most common serotype was S. Infantis 38 (82.6%) from broiler litter; followed by S. Kitenge 3 (6.5 %) from fecal sample. The highest occurrence of resistance observed for penicilline (46/46, %100), lincomycin (43/46, 93.5%) and 42 isolates (43/46, 93.5%) exhibited MDR. The overall occurrence of class 1, 2 and 3 integrons carrying Salmonella in tested samples were 63.04% (29/46), 43.5% (20/46) and 84.8% (39/46) respectively. Out of the 27 isolates produced an ESBL, mostly CTX and TEM. On 46 Salmonella isolates, in 16 (34.8%) Tcr’ genes were determined. Genotypic and phenotipic detection of ESBL genes found within integrons from Salmonella isolates from different sources (broiler and cattle) can provide powerful information about health and economic risk associated with transferable multidrug resistance.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 345-355
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of antibiotic susceptibility of Legionella pneumophila isolated from water systems in Poland
Autorzy:
Sikora, Agnieszka
Gładysz, Iwona
Kozioł-Montewka, Maria
Wójtczak-Bobin, Małgorzata
Stańczak, Tomasz
Matuszewska, Renata
Krogulska, Bożena
Powiązania:
https://bibliotekanauki.pl/articles/988953.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
legionella pneumophila
susceptibility
e-test
minimum inhibitory concentration
Opis:
Introduction and objective. Several studies have reported therapy failures in patients with legionnaires’disease; however, antimicrobial resistance of clinical and environmental isolates of Legionella spp. has not yet been documented. Routine susceptibility testing of Legionella spp. is not recommended because of difficulties in determining standard minimal inhibitory concentration values. The purpose of this study was to analyze the antimicrobial susceptibility of Legionella pneumophila. strains isolated from a water supply system. Materials and method. Twenty-eight isolates of L. pneumophila (16 – L. pneumophila SG 1, 12 – L. pneumophila SG 2–14) obtained from water systems in public buildings in Poland were tested. Susceptibility testing was performed using the E-test method. The tested antibiotic were azithromycin, ciprofloxacin, and rifampicin. The medium used for the susceptibility testing was BCYE-, a special medium for Legionella cultivation. Results. Among the tested strains, L. pneumophila was the only one resistant to azithromycin. It was a strain of L. pneumophila SG 2–14 isolated from the water system in a sanitorium. All isolates were found to be sensitive to ciprofloxacin and rifampicin. However, the azithromycin-resistant strain exhibited higher ciprofloxacin and rifampicin MIC (1.5 μg/ml, and 0.19 μg/ml, respectively). The MIC50 for azithromycin, ciprofloxacin, and rifampicin were 0,032, 0,125, and 0,003 μg/ml, respectively. The MIC90 for azithromycin, ciprofloxacin, and rifampicin were 0,032, 0,125, and 0,003 μg/ml, respectively. Conclusions. Azithromycin resistance was found in one strain of L. pneumophila SG 2–14, but the resistance mechanism is unknown and needs further study. It is possible that therapeutic failures in Legionnaires’ disease may be associated with bacterial resistance which should be taken into account. The antibiotic sensitivity testing described in this study could be helpful in detecting the resistance of clinical L. pneumophila isolates. Ciprofloxacin and rifampicin have good in vitro activity against environmental L. pneumophila SG 1 and SG 2–14 in Poland.
Źródło:
Annals of Agricultural and Environmental Medicine; 2017, 24, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia
Autorzy:
Geta, Kindu
Kebede, Ameha
Chemedissa, Meseret
Powiązania:
https://bibliotekanauki.pl/articles/1076786.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibiotics
Antimicrobial resistance
B. cereus
Debre Markos
E. coli
Enterobacter
Fruit juices
Klebsiella
Pathogens
Ps. aeruginosa
Salmonella
Shigella
Staphylococcus aureus
Opis:
The prevalence of antimicrobial resistance among food pathogens has increased during recent decades. In this work, pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., Shigella spp., and E. coli were isolated following standard methods. The bacterial isolates were then tested for their sensitivity to common antibiotics using the disc diffusion method on Mueller-Hinton Agar. All of the pathogenic bacteria were found to be resistant to erythromycin and almost all were sensitive to penicillin.
Źródło:
World News of Natural Sciences; 2019, 24; 365-371
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic Susceptibility Profiling of Bacterial Biodiversity Associated with Urinary Tract Infections
Autorzy:
Prasad, Bhairav
Arora, Priya
Powiązania:
https://bibliotekanauki.pl/articles/1193375.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibiotics
Escherichia coli
Klebsiella
Pseudomonas
Sensitivity profiling
Staphylococcus
Urinary system
Urinary tract infections
Opis:
Urinary tract infections (UTI’s) are a bacterial infection that affects any part of the urinary tract. Urinary tract infection is one of the major diseases that affect people of all age groups and sexes and can be separated into asymptomatic and symptomatic. The normal urinary tract is sterile but gets infected with the normal flora and opportunistic pathogens. Urinary tract infections (UTIs) are a serious health problem affecting millions of people each year. This is the second most common type of infection in the body. In the present study 20 morphologically different bacterial isolates were recovered from 50 different urine samples from UTI’s patient. The prevalence of the isolates was varied with E. coli (40%), followed by Staphylococcus spp., Pseudomonas spp. and Klebsiella spp. each of (20%) respectively. All the isolates of different bacteria were tested for antibiotic sensitivity patterns against common antibiotics viz. Ampicillin, Tetracycline, Chloramphenicol, Vancomycin, Nitrofurontione and Ciprofloxacin with potencies of 30 μg each. The sensitive pattern of the isolates towards antibiotic Nitrofurontoine was (75%) followed by Tetracycline (55%) and Ciprofloxacin (45%). Intermediate activity was reported against Ampicillin (45%), Chloramphenicol and vancomycin (40%). Resistant pattern shown against vancomycin (60%), Chloramphenicol (45%), while both tetracycline and Ciprofloxacin shown (30%) against the UTI’s isolates. Therefore, drugs like Nitrofurontoine, Tetracycline and Ciprofloxacin are the most effective choices against the common UTI’s isolates.
Źródło:
World Scientific News; 2021, 155; 129-139
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic susceptibility of methicillin - resistant and methicillin - suspectible coagulase-negative staphylococci isolated from bovine mastitis
Autorzy:
Bochniarz, M.
Wawron, W.
Powiązania:
https://bibliotekanauki.pl/articles/32123.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the present study was to evaluate the antibiotic susceptibility of methicillin-susceptible (MS) and methicillin-resistant (MR) coagulase-negative Staphylococcus (CNS) strains isolated from milk of cows with mastitis. The study was conducted on 100 CNS strains (20 MRCNS and 80 MSCNS) isolated from milk samples of 86 cows from the Lublin (Poland) region farms. Antibiotic susceptibility of microorganisms was evaluated using the disc-diffusion method on the Mueller-Hinton agar according to the guidelines of the National Committee for Clinical Laboratory Standards (NCCLS). The highest efficacy against MSCNS was demonstrated for cephalosporin antibiotics, i.e. cefacetril (91.3%), ceftiofur (67.5%), cefoperazone (66.3%) and cephalexin (60.0% of susceptible MSCNS strains). Moreover, a high percentage of vancomycin-susceptible strains was demonstrated (83. 8%). The activity of combination of amoxicillin with clavulanic acid and gentamicin was found weaker (63.8% and 61.3% of susceptible strains, respectively). About 50.0% of MSCNS were susceptible to erythromycin, enrofloxacine and amoxicillin. A large proportion of CNS was resistant to neomycin, penicillin, tetracycline, streptomycin, lincomycin and ampicillin (28.8%, 30.0%, 31.3%, 31.3%, 33.8% and 33.8% of susceptible strains, respectively). The highest percentage of MRCNS was susceptible to vancomycin (75.0%), erythromycin (65.0%) and streptomycin (50.0%). Their susceptibility to enrofloxacine (35.0%) as well as gentamicin and tetracycline (30.0%) was markedly lower. The lowest activity was found for lincomycin and neomycin (20.0% of susceptible MRCNS strains, each).
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in Poland between 2004 and 2008
Autorzy:
Markowska-Daniel, I
Urbaniak, K.
Stepniewska, K.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30384.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Polska
pig
respiratory tract
bacteria
isolation
antibiotic susceptibility
animal health
productivity
animal production
Opis:
Antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test, ß-lactams showed the best activity against Streptococcus suis (S. suis) (> 99% of susceptible strains). The lowest sensitivity of S. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). Isolates of Escherichia coli (E. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentamicin and norfloxacin (over 74%). The lowest susceptibility of E. coli was demonstrated to tiamulin and penicillin (11.3% and 1.9%, respectively). Over 80% of Actinobacillus pleuropneumoniae (App) strains were susceptible to all antibiotics tested. The highest resistance of App, but demonstrated by below 20% of tested isolates only, was evidenced to neomycin and LxS. Isolates of Pasteurella multocida (Pm), Haemophilus parasuis (Hps) and Arcanobacterium pyogenes (A. pyogenes) were highly susceptible to the most antibiotics included in the analysis. The comparison of the in vitro susceptibility of pathogens to the chemotherapeutics used on Polish farms for the therapy of bacterial infection of pigs within the last five years and the last 10 years, showed an increasing percent of E. coli and S. suis strains resistant to commonly used antibiotics. It is also shown that Pm, Hps, App and A. pyogenes isolates were continuously susceptible to the most chemotherapeutics applied.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 29-36
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic resistance and siderophores production by clinical Escherichia coli strains
Autorzy:
Khazaal, Mohamed T.
El-Hendawy, Hoda H.
Mabrouk, Mona I.
Faraag, Ahmed H.I.
Bakkar, Marwa R.
Powiązania:
https://bibliotekanauki.pl/articles/16648088.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
siderophores
Escherichia coli
clinical strains
antibiotic susceptibility
Opis:
The phenomenon of antibiotic resistance has dramatically increased in the last few decades, especially in enterobacterial pathogens. Different strains of Escherichia coli have been reported to produce a variety of structurally different siderophores. In the present study, 32 E. coli strains were collected from different clinical settings in Cairo, Egypt and subjected to the antibiotic susceptibility test by using 19 antibiotics belonging to 7 classes of chemical groups. The results indicated that 31 strains could be considered as extensively drug-resistant and only one strain as pan drug-resistant. Siderophores production by all the tested E. coli strains was determined qualitatively and quantitatively. Two E. coli strains coded 21 and 49 were found to be the most potent siderophores producers, with 79.9 and 46.62%, respectively. Bacterial colonies with cured plasmids derived from strain 49 showed susceptibility to all the tested antibiotics. Furthermore, E. coli DH5α cells transformed with the plasmid isolated from E. coli strain 21 or E. coli strain 49 were found to be susceptible to ansamycins, quinolones, and sulfonamide groups of antibiotics. In contrast, both plasmid-cured and plasmid-transformed strains did not produce siderophores, indicating that the genes responsible for siderophores production were located on plasmids and regulated by genes located on the chromosome. On the basis of the obtained results, it could be concluded that there is a positive correlation between antibiotic resistance, especially to quinolones and sulfonamide groups, and siderophores production by E. coli strains used in this study.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 2; 169-184
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 16

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