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Wyświetlanie 1-15 z 109
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial spectrum of four compounds from yeasts in koumiss
Autorzy:
Chen, Y.J.
Du, C.G.
Guo, Y.Q.
Zhao, Y.F.
Aorigele, C.
Wang, C.J.
Simujide, H.
Aqima, W.
Zhang, X.Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087160.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Kluyveromyces marxianus
Saccharomyces cerevisiae
Escherichia coli
minimum inhibition concentration
minimum bactericidal concentration
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 167-173
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of fuzzy fault tree analysis and noisy-OR gate bayesian network for navigational risk assessment in Qingzhou Port
Autorzy:
Zhao, C.
Wu, B.
Yip, T. L.
Lv, J.
Powiązania:
https://bibliotekanauki.pl/articles/2063967.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
risk assessment
Bayesian Network Model
navigational risk
navigational risk assessment
Port of Qingzhou
fuzzy fault tree
noisy-OR gate
navigational accidents
Opis:
Collisions and groundings account for more than 80% among all types of maritime accidents, and risk assessment is an essential step in the formal safety assessment. This paper proposes a method based on fuzzy fault tree analysis and Noisy-OR gate Bayesian network for navigational risk assessment. First, a fault tree model was established with historical data, and the probability of basic events is calculated using fuzzy sets. Then, the Noisy-OR gate is utilized to determine the conditional probability of related nodes and obtain the probability distribution of the consequences in the Bayesian network. Finally, this proposed method is applied to Qinzhou Port. From sensitivity analysis, several predominant influencing factors are identified, including navigational area, ship type and time of the day. The results indicate that the consequence is sensitive to the position where the accidents occurred. Consequently, this paper provides a practical and reasonable method for risk assessment for navigational accidents.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2021, 15, 3; 765--771
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chinese cabbage BrMYB34.2 transcription factor regulates indolic glucosinolates biosynthesis in Arabidopsis
Autorzy:
Zhao, Y.
Zhang, Y.
Guo, X.
Ma, Y.
Zhang, P.
Liu, H.
Liu, G.
Guo, J.
Powiązania:
https://bibliotekanauki.pl/articles/12705984.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Glucosinolates (GS) are a group of sulfur- and nitrogen-rich plant secondary metabolites that originate from amino acids and exist mainly in plants in the order Brassicales, such as Arabidopsis thaliana (Arabidopsis) and Chinese cabbage (Brassica rapa ssp. pekinensis). To date, several regulatory components responsible for GS biosynthesis have been identified in Arabidopsis. However, the functions of GS biosynthesis regulators in Chinese cabbage have not been clarified. In our current study, a putative ATR1/MYB34 orthologous gene, BrMYB34.2, was isolated from Chinese cabbage leaves. To investigate the function of this gene, we engineered Arabidopsis plants that overexpress BrMYB34.2 ectopically and phenotypic analysis was performed. Moreover, we assayed the accumulation levels of indolic GS (IGS) and aliphatic glucosinolates in transgenic plants and test the expression of key genes of IGS biosynthesis and tryptophan synthesis by Real-time quantitative PCR. And further analysed the resistance of transgenic plants in 5MT stress treatment. The results indicate that ectopic expression of the BrMYB34.2 gene in Arabidopsis was able to up-regulate the accumulation level of IGS due to the increased expression of IGS and Trp biosynthetic genes. Moreover, overexpression of BrMYB34.2 conferred Arabidopsis 5MT resistance. These results suggest that the BrMYB34.2 gene may function as one of the regulators of IGS and Trp biosynthesis in Chinese cabbage.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 85-95
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Autorzy:
Zhao, L.Y.
Niu, J.H.
Gao, X.L.
Liu, C.N.
Liu, S.M.
Jiang, N.
Lv, X.P.
Zheng, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/2087340.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
F4 fimbriae
isothermal amplification
rapid detection
clinical samples
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 143-152
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improvements in timber production of Chinese fir (Cunninghamia lanceolata) per unit forest area in China via tree breeding: Status and challenges
Autorzy:
Zhou, B.
Peng, D.
Zhao, Q.
Yangnan, S.
Yang, S.
Yang, F.
Qu, G.
Tang, W.
Ou, J.
Xiang, W.
Xiang, J.
Deng, X.
Powiązania:
https://bibliotekanauki.pl/articles/2077649.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Cunninghamia lanceolata
genetic improvement
timber volumes per unit forest area
wood properties
proposals
recommendations
Opis:
Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a fast-growing tree species that is widely planted for timber production in southern China. Many high-quality breeding systems for Chinese fir have been developed using genetic methods. Here, we systematically review progress in increasing Chinese fir timber volumes per unit forest area in China, using genetic methods. We comprehensively assess the objec- tives, strategies, and primary accomplishments of the first to fourth generations of a Chinese fir breeding program. Furthermore, we propose a genetic improvement program and protocol for Chinese fir, and out- line several recommendations with respect to the main challenges associated with using genetic methods to increase timber yields. Finally, we present a series of proposals to increase Chinese fir timber production in China. This review offers insight into genetic improvements that have increased timber volumes of Chi- nese fir over four generations of breeding, and provides direction for future genetic improvements to wood properties.
Źródło:
Dendrobiology; 2020, 83; 43-51
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
n-sided polygonal hybrid finite elements involving element boundary integrals only for anisotropic thermal analysis
Autorzy:
Cao, R. F.
Zhao, X. J.
Lin, W. Q.
Wang, H.
Powiązania:
https://bibliotekanauki.pl/articles/38597074.pdf
Data publikacji:
2020
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
hybrid finite element
polygon
non-conforming mesh
fundamental solution
anisotropic material
heat conduction
Opis:
As a combination of the traditional finite element method and boundary element method, the n-sided polygonal hybrid finite element method with fundamental solution kernels, named as HFS-FEM, is thoroughly studied in this work for two-dimensional heat conduction in fully anisotropic media. In this approach, the unknown temperature field within the polygon is represented by the linear combination of anisotropic fundamental solutions of problem to achieve the local satisfaction of the related governing equations, but not the specific boundary conditions and the continuity conditions across the element boundary. To tackle such a shortcoming, the frame temperature field is independently defined on the entire boundary of the polygonal element by means of the conventional one-dimensional shape function interpolation. Subsequently, by the hybrid functional with the assumed intra- and inter-element temperature fields, the stiffness equation can be obtained including the line integrals along the element boundary only, whose dimension is reduced by one compared to the domain integrals in the traditional finite elements. This means that the higher computing efficiency is expected. Moreover, any shaped polygonal elements can be constructed in a unified form with the same fundamental solution kernels, including convex and non-convex polygonal elements, to provide greater flexibility in meshing effort for complex geometries. Besides, the element boundary integrals endow the method higher versatility with a non-conforming mesh in the pre-processing stage of the analysis over the traditional FEM. No modification to the HFS-FEM formulation is needed for the non-conforming mesh and the element containing hanging nodes is treated normally as the one with more nodes. Finally, the accuracy, convergence, computing efficiency, stability of non-convex element, and straightforward treatment of non-conforming discretization are discussed for the present n-sided polygonal hybrid finite elements by a few applications in the context of anisotropic heat conduction.
Źródło:
Archives of Mechanics; 2020, 72, 2; 109-137
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on radiation characteristics of plasma Yagi antenna based on AIS base station in ships’ routeing waters
Autorzy:
Sun, Y.
Chen, Y.
Kong, F.
Wei, Y.
Zhan, F.
Zhao, J. S.
Powiązania:
https://bibliotekanauki.pl/articles/117021.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
radio navigation
radiation characteristics
plasma Yagi antenna
ships routeing
AIS base station
AIS based shipping routes
ships routeing waters
Automatic Identification System (AIS)
Opis:
A Yagi plasma antenna model was established by HFSS according to the relationship between plasma dielectric constant and electron density. The patterns were simulated by changing plasma parameters and the number of director dipoles. Results show that when the passive vibrators were switched off, the antenna is omnidirectional antenna. The directionality increases with the increase of the number of passive dipole and the main lobe of which narrows down. Then the plasma Yagi antenna model is established by plasma tube, the gain changed by changing the number of passive dipoles, so the plasma Yagi antenna has a very good reconfigurability. Results prove that the feasibility of the plasma Yagi antenna can be used on AIS base station of Ships’ Routeing waters. It can promote the communication and capability of maritime supervision in Ships’ Routeing waters.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2020, 14, 1; 179-184
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seismic damage behaviour of aeolian sand reinforced concrete columns with I-shaped structural steel
Autorzy:
Wang, Y. H.
Wang, H. Y.
Wang, Y. P.
Zhao, M.
Qi, J.
Huo, G. Z.
Liu, P. Q.
Powiązania:
https://bibliotekanauki.pl/articles/1849772.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
piasek eoliczny
słup żelbetowy
stal konstrukcyjna
przekrój I
zachowanie
uszkodzenie sejsmiczne
model uszkodzenia
aeolian sand
column
I-shaped steel
structural steel
seismic damage
behaviour
damage model
Opis:
To promote the application of aeolian sand resources for steel-concrete composite structures, an aeolian sand reinforced concrete column with I-shaped structural steel is proposed in this study. Four specimens are designed and manufactured with different replacement rates of aeolian sand. The seismic behaviour and damage evolution process of the specimens are studied by low-cycle repeated loading tests. Based on the test results, the mechanical characteristics, failure modes, hysteresis curves, skeleton curves, energy dissipation capacity, displacement ductility, and stiffness degradation of the specimens with different replacement rates of aeolian sand are analysed. In addition, the effects of the design parameters on the seismic behaviour of the specimens are also studied. The results show that the indexes of the seismic behaviour can be significantly improved by adding steel. Moreover, a revised damage model is proposed, to better reflect the evolution law of seismic damage of aeolian sand reinforced concrete columns with steel. The proposed model can provide an important reference for seismic damage assessment of the columns.
Źródło:
Archives of Civil Engineering; 2020, 66, 4; 633-650
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
Autorzy:
Zhang, J.
Li, Y.
Xu, E.
Jiang, L.
Tang, J.
Li, M.
Zhao, X.
Chen, G.
Zhu, H.
Yu, X.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087465.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial communities
broilers
high-throughput sequencing
particulate matter
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 495-504
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Derivation of formulas in spherical trigonometry based on rotation matrix
Autorzy:
Hsieh, T. H.
Wang, S. Z.
Liu, W.
Zhao, J. S.
Chen, C. L.
Powiązania:
https://bibliotekanauki.pl/articles/116775.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
spherical trigonometry
rotation matrix
spherical triangle
formulas of spherical triangle
nautical mathematics
geometric method
spherical navigation
Astronavigation
Opis:
The formulas of spherical triangle, which are widely used to solve various navigation problems, are the important basic knowledge of nautical mathematics. Because the sine rules and the cosine rules for the sides are the fundamental formulas to derive the other spherical triangle formulas, they are also called the genetic codes of the spherical triangle formulas. In the teaching process, teachers usually use the geometric method to derive and prove these fundamental formulas. However, the derivation of geometric methods is complicated and difficult to understand. To improve the teaching process, this paper proposes the three-dimensional rotation method, which is based on conversion of two cartesian coordinate frames using the rotation matrices. This method can easily and simultaneously derive the sine rules, the cosine rules for the sides, and the five-part formulas (I), and is also helpful to solve different kinds of spherical navigation problems.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 3; 553-558
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental study of an anomalous hollow beam with orbital angular momentum
Autorzy:
Lu, Xingyuan
Zhao, Chengliang
Hoenders, Bernhard J.
Yang, Yuanjie
Cai, Yangjian
Powiązania:
https://bibliotekanauki.pl/articles/173518.pdf
Data publikacji:
2019
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
anomalous hollow beam
orbital angular momentum
focused intensity
Fourier transform
Opis:
Anomalous hollow beam has potential applications in the optical trapping and free space optical communications, etc. It is noted that, thus far, although a large number of studies have been carried out in this field, most of them are theoretical studies and only quite a few cases have experimental results. Here, we experimentally study the generating of anomalous hollow beam carrying orbital angular momentum, and measure its topological charge. We show that the number of dark rings in the Fourier transform of intensity patterns is equal to the topological charge. The experimental results agree well with the simulations.
Źródło:
Optica Applicata; 2019, 49, 2; 217-226
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, recombinant expression, and purification of osteocalcin in sika deer (Cervus nippon) antler
Autorzy:
Li, X.
Liu, M.
Bai, X.
Li, Y.
Zhao, Y.
Wang, S.
Wang, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087587.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Cervus nippon
osteocalcin
molecular cloning
expression
purification
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 143-150
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ontogenetic stages of ceratopsian dinosaur Psittacosaurus in bone histology
Autorzy:
Zhao, Q.
Benton, M.J.
Hayashi, S.
Xu, X.
Powiązania:
https://bibliotekanauki.pl/articles/20498.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
The early ceratopsians Psittacosaurus and Protoceratops have provided important information on dinosaurian development because of abundant specimens of adults, subadults, juveniles, and even hatchlings. Here we present new data and methods for identifying key growth stages from bone histology. Previous studies on Psittacosaurus lujiatunensis from the Early Cretaceous Jehol Biota of China did not present in-depth analysis of growth patterns. Based on a histological study of 43 thin sections from 17 individuals of this species, we recognize four histological ontogenetic stages, i.e., hatchling, juvenile, sub-adult, and adult, but no fully-grown stage. We estimate life history and longevity from diaphyseal growth line counts and other features of histology. We show that P. lujiatunensis grew fast in early stages (hatchling, juvenile, and subadult), according to the density of vascular canals and the different type of bone tissue; the deposition of parallel fibred bone tissue in the outer cortex of the subadult stage indicates that growth rate was slowing down. We introduce a new graphical method to estimate the occurrence and volumes of vascular canals from thin sections more accurately than current two-dimensional approaches.
Źródło:
Acta Palaeontologica Polonica; 2019, 64, 2
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pharmacokinetics of the recombinant ovine interferon-tau in lambs
Autorzy:
Zhao, J.
Yu, H.Y.
Zhao, Y.
Li, S.Q.
Fu, X.L.
Zhou, W.
Xia, B.B.
Wang, M.L.
Chen, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087588.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bioavailability
ovine interferon-τ
cytopathic effect inhibition assay
pharmacokinetic study
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 75-82
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 109

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