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    Wyświetlanie 1-6 z 6
    Tytuł:
    Protective antigen domain 4 of Bacillus anthracis as a candidate for use as vaccine for anthrax
    Autorzy:
    Żakowska, D.
    Graniak, G.
    Rutyna, P.
    Naylor, K.
    Glowacka, P.
    Niemcewicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085049.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Bacillus anthracis
    cloning pag gene
    domain 4
    protective antigen
    protein
    recombinant
    vaccine
    Opis:
    Existing research for using the protective antigen (PA) of Bacillus anthracis as a vaccine component shows that protection against anthrax may be obtained using fragments of this protein. The aim of the research is to check whether the selected protein fragment of the protective antigen (domain 4) encoded by an appropriate nucleotide sequence of gene pag of B. anthracis, was expressed in the bacterial system of E. coli. In order to examine the selected sequence of the pag gene, a PCR reaction and a highly effective TOPO cloning strategy were used, followed by purification of the recombinant proteins and their detection by a western-blot method. In the planning of the PA4 antigen expression a higher level of effectiveness in production of small protein – domain 4 – was anticipated. As a result, the 139 amino acids protein fragment of B. anthracis PA (domain 4) was isolated. The research may have found the basis for in vivo research aimed at finding potential anthrax vaccine components.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 3; 392-395
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Q fever - selected issues
    Autorzy:
    Bielawska-Drozd, A.
    Cieslik, P.
    Mirski, T.
    Bartoszcze, M.
    Knap, J.P.
    Gawel, J.
    Zakowska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/49820.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Q fever
    animal species
    man
    Coxiella burnetii
    intracellular parasite
    parasite
    htpAB gene
    IS1111 gene
    genome
    plasmid
    pathomechanism
    infection
    bioterrorism
    epidemiology
    epizootiology
    treatment
    prophylaxis
    laboratory diagnostics
    serological method
    molecular diagnostics
    Opis:
    Q fever is an infectious disease of humans and animals caused by Gram-negative coccobacillus Coxiella burnetii, belonging to the Legionellales order, Coxiellaceae family. The presented study compares selected features of the bacteria genome, including chromosome and plasmids QpH1, QpRS, QpDG and QpDV. The pathomechanism of infection – starting from internalization of the bacteria to its release from infected cell are thoroughly described. The drugs of choice for the treatment of acute Q fever are tetracyclines, macrolides and quinolones. Some other antimicrobials are also active against C. burnetii, namely, telitromycines and tigecyclines (glicylcycline). Q-VAX vaccine induces strong and long-term immunity in humans. Coxevac vaccine for goat and sheep can reduce the number of infections and abortions, as well as decrease the environmental transmission of the pathogen. Using the microarrays technique, about 50 proteins has been identified which could be used in the future for the production of vaccine against Q fever. The routine method of C. burnetii culture is proliferation within cell lines; however, an artificial culture medium has recently been developed. The growth of bacteria in a reduced oxygen (2.5%) atmosphere was obtained after just 6 days. In serology, using the IF method as positive titers, the IgM antibody level >1:64 and IgG antibody level >1:256 (against II phase antigens) has been considered. In molecular diagnostics of C. burnetii infection, the most frequently used method is PCR and its modifications; namely, nested PCR and real time PCR which detect target sequences, such as htpAB and IS1111, chromosome genes (com1), genes specific for different types of plasmids and transposase genes. Although Q fever was diagnosed in Poland in 1956, the data about the occurrence of the disease are incomplete. Comprehensive studies on the current status of Q fever in Poland, with special focus on pathogen reservoirs and vectors, the sources of infection and molecular characteristics of bacteria should be conducted.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bacillus anthracis infections – new possibilities of treatment
    Autorzy:
    Zakowska, D.
    Bartoszcze, M.
    Niemcewicz, M.
    Bielawska-Drozd, A.
    Knap, J.
    Cieslik, P.
    Chomiczewski, K.
    Kocik, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51666.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2015, 22, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microbiological analysis of bioaerosols collected from Hospital Emergency Departments and ambulances
    Autorzy:
    Bielawska-Drózd, A.
    Cieślik, P.
    Bohacz, J.
    Kornillowicz-Kowalska, T.
    Żakowska, D.
    Bartoszcze, M.
    Wlizło-Skowronek, B.
    Winnicka, I.
    Brytan, M.
    Kubiak, L.
    Skopińska-Różewska, E.
    Kocik, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081735.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Button Sampler
    bioaerosol
    Hospital Emergency Departments
    ambulances
    bacteria
    fungi
    Opis:
    Introduction and objective. The goal of the study was a microbiological, qualitative and quantitative analysis of bioaerosol at the workplace of medical personnel (Health Emergency Departments (HEDs), ambulances), and comparative administration offices with an expected neutral occupational exposure to biological agents measured with individual Button Sampler. Materials and method. Personal sampling was performed with Button Sampler instrument loaded with gelatine filters in 10 HEDs, in 9 ambulances and in 9 offices to assess the occupational biological agents’ exposure in air. Sampling was conducted from March until April 2016. Samples were quantitatively assessed for viable and total number of bacteria and fungi. Routine procedures for microbiological diagnostics were implemented. Data were analysed using Kruskal–Wallis and Mann-Whitney statistical tests with α=0.05. P value less than 0.05 were considered significant. Results. At the workplaces assessed, the concentrations of viable microorganisms in HEDs were 1.3 × 102 – 4.2 × 103 CFU/m3 for bacteria, 3.4 × 100 – 8.1 × 101 CFU/m3 for fungi; in ambulances 1.3 × 102 – 1.4 × 103 CFU/m3 (bacteria), 6.7 × 100 – 6.5 × 102 CFU/m3 (fungi) and in offices 4.2 × 101 – 5.0 × 103 CFU/m3 (bacteria), 0 – 7.9 × 102 CFU/m3 (fungi). In outdoor air, the number of microorganisms reached the level: 1.0 × 102 – 5.9 × 102 CFU/m3 for bacteria and 1.5 × 102 – 8.2 × 102 CFU/m3 for fungi. The predominant isolated bacteria were Gram-positive cocci. The prevalent fungi species belonged to the genus Aspergillus and Penicillium. Conclusions. The quantitative assessment of examined indoor air was similar to control outdoor air, and were relatively low. The level of microbiological contamination did not exceed 5 × 103 CFU/m3 which is recommended as an admissible level in public spaces in Poland.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 274-279
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Utilization of Cereal and Milling Industry Products as Raw Materials for the Manufacture of Biopolymer Technical Products
    Zagospodarowanie surowców przemysłu mączno-zbożowego do wytwarzania biopolimerowych produktów technicznych
    Autorzy:
    Ciechańska, D.
    Kaszuba, L.
    Żakowska, H.
    Sulak, K.
    Mik, T.
    Janiga, M.
    Wietecha, J.
    Gutowska, A.
    Kapuśniak, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/949428.pdf
    Data publikacji:
    2016
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
    Tematy:
    cereal milling industry
    functional biopolymers
    bio-packaging materials
    bio-thermoplastics
    bio-adhesives
    przemysł młynarski
    biopolimery funkcyjne
    produkty biopolimerowe
    bio-kleje
    Opis:
    The pressure which is being exerted on manufacturers by the users of consumer goods towards more environmentally friendly applications, favours the use of already existing environmentally friendly raw materials or the search for new ones. One of the promising directions for research is aimed at developing new packaging materials based on biopolymers. Research recently conducted made it possible to use hydrothermally pre-treated starch/protein raw material in the manufacture of a new generation of biopolymer materials. Biopolymer products obtained by various modifications were used to produce an exemplary batches of mouldings and packaging materials (films, corrugated cardboard).
    Nacisk wywierany przez użytkowników dóbr konsumpcyjnych, ukierunkowany na zastosowanie bardziej proekologiczne, powoduje stosowanie już istniejących lub poszukiwanie nowych surowców i materiałów przyjaznych środowisku. Jednym z kierunków rokujących duże nadzieje są badania mające na celu opracowanie nowych materiałów opakowaniowych do wytworzenia, których wykorzystywane będą surowce biopolimerowe. Wykonane prace badawcze pozwoliły na wykorzystanie wstępnie zmodyfikowanego hydrotermicznie surowca skrobiowo-białkowego do wytworzenia nowej generacji materiałów biopolimerowych. Uzyskane w wyniku modyfikacji produkty posłużyły, z kolei, do wytworzenia demonstracyjnych partii materiałów biopolimerowe - folii, kształtek oraz klejów do tektur wielowarstwowych.
    Źródło:
    Fibres & Textiles in Eastern Europe; 2016, 6 (120); 175-180
    1230-3666
    2300-7354
    Pojawia się w:
    Fibres & Textiles in Eastern Europe
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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