Wszystkie pola: Urbańska, Krystyna - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Optimization of Western blotting analysis for the isolation and detection of membrane xenobiotic transporter ABCG2
Autorzy:: Szczygieł, Małgorzata
Markiewicz, Marcin
Szafraniec, Milena
Zuziak, Roxana
Urbańska, Krystyna
Fiedor, Leszek
Powiązania:: https://bibliotekanauki.pl/articles/1038582.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Xenobiotic transporter
ABCG2
BCRP
Western blotting
Opis:: All organisms are exposed to numerous stress factors, which include harmful xenobiotics. The diversity of these compounds is enormous, thus in the course of evolution diverse biological defense mechanisms at various levels of organization have developed. One of them engages an evolutionarily conserved family of transporters from the ABC superfamily, found in most species - from bacteria to humans. An important example of such a transporter is the breast cancer resistance protein (BCRP/ABCG2), a typical integral membrane protein. It plays a key role in the absorption, distribution and elimination of a wide variety of xenobiotics, including drugs used in chemotherapy, and is involved in multidrug resistance. It also protects against phototoxic chlorophyll derivatives of dietary origin. BCRP is a hemitransporter which consists of one transmembrane domain, made of six alpha-helices forming a characteristic pore structure, and one ATP-binding domain, which provides the energy from ATP hydrolysis, required for active transport of the substrates. The isolation of BCRP is still not an easy task, because its insolubility in water and the presence of membrane rafts pose serious methodological and technical challenges during the purification. The aim of this study was to optimize the methods for detection and isolation of BCRP-enriched fractions obtained from animal tissue samples. In this report we describe an optimization of isolation of a BCRP-enriched membrane fraction, which is suitable for further protein quantitative and qualitative analysis using the molecular biology tools.
Źródło:: Acta Biochimica Polonica; 2017, 64, 3; 437-443
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Indocyanine green as a prospective sensitizer for photodynamic therapy of melanomas.
Autorzy:: Urbanska, Krystyna
Romanowska-Dixon, Bożena
Matuszak, Zenon
Oszajca, Janusz
Nowak-Sliwinska, Patrycja
Stochel, Grażyna
Powiązania:: https://bibliotekanauki.pl/articles/1043767.pdf
Data publikacji:: 2002
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: melanoma
photodynamic therapy
indocyanine green
Opis:: Spectroscopic, photochemical and biological properties of indocyanine green (ICG) are presented. Light over 800 nm is effectively absorbed by ICG. This property as well as photochemical behaviour of ICG make it a very suitable dye for photodynamic treatment of melanoma cells. Cytotoxicity of ICG itself and the effect of photodynamic therapy (PDT) were evaluated by following the growth of human (SKMEL 188) and mouse (S91) melanoma cells. The surviving fraction of the cells irradiated (λex = 830 nm) vs non-irradiated, treated with the same dose of ICG, is significantly decreased (5- to 10-fold). These results show that ICG is a very promising dye for photodynamic therapy of melanomas.
Źródło:: Acta Biochimica Polonica; 2002, 49, 2; 387-391
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Tritolylporphyrin dimer as a new potent hydrophobic sensitizer for photodynamic therapy of melanoma.
Autorzy:: Drzewiecka, Agnieszka
Urbańska, Krystyna
Matuszak, Zenon
Pineiro, Marta
Arnaut, Luis
Habdas, Jan
Ratuszna, Alicja
Stochel, Grażyna
Powiązania:: https://bibliotekanauki.pl/articles/1044198.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: melanoma
porphyrins
photodynamic therapy
Opis:: We report the synthesis, photochemical and photophysical properties and preliminary studies on biological effect of a new tritolylporphyrin dimer (T-D). Absorption and emission properties of T-D suggest its possible use in photodynamic therapy. T-D is capable of singlet oxygen production with 0.8 quantum yield. It also has a high photostability. The photodynamic properties of the dimer were examined following the growth of SKMEL 188 (human melanoma) cells irradiated with red light (cut off <630 nm). The surviving fraction of the cells decreased about 3-fold (vs. non-irradiated cells) for an 81 J/cm2 dose. Our results suggest that tritolylporphyrine dimer T-D may be an interesting hydrophobic sensitizer for photodynamic therapy.
Źródło:: Acta Biochimica Polonica; 2001, 48, 1; 277-282
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Metastasis inhibition after proton beam, β- and γ-irradiation of melanoma growing in the hamster eye
Autorzy:: Romanowska-Dixon, Bożena
Elas, Martyna
Swakoń, Jan
Sowa, Urszula
Ptaszkiewicz, Marta
Szczygieł, Małgorzata
Krzykawska, Martyna
Olko, Paweł
Urbańska, Krystyna
Powiązania:: https://bibliotekanauki.pl/articles/1039523.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: metastases
melanoma
ocular
proton beam
iodine-125
ruthenium-106
Opis:: Standard ocular tumor treatment includes brachytherapy, as well as proton therapy, particularly for large melanoma tumors. However, the effects of different radiation types on the metastatic spread is not clear. We aimed at comparing ruthenium (106Ru, emitting β electrons) and iodine (125I, γ-radiation) brachytherapy and proton beam therapy of melanoma implanted into the hamster eye on development of spontaneous lung metastases. Tumors of Bomirski Hamster Melanoma (BHM) implanted into the anterior chamber of the hamster eye grew aggressively and completely filled the anterior chamber within 8-10 days. Metastases, mainly in the lung, were found in 100% of untreated animals 30 days after enucleation. Tumors were irradiated at a dose of 3-10 Gy with a 106Ru plaque and at a dose of 6-14 Gy using a 125I plaque. The protons were accelerated using the AIC-144 isochronous cyclotron operating at 60 MeV. BHM tumors located in the anterior chamber of the eye were irradiated with 10 Gy, for the depth of 3.88 mm. All radiation types caused inhibition of tumor growth by about 10 days. An increase in the number of metastases was observed for 3 Gy of β-irradiation, whereas at 10 Gy an inhibition of metastasis was found. γ-radiation reduced the metastatic mass at all applied doses, and proton beam therapy at 10 Gy also inhibited the metastastic spread. These results are discussed in the context of recent clinical and molecular data on radiation effects on metastasis.
Źródło:: Acta Biochimica Polonica; 2013, 60, 3; 307-311
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Pulmonary metastases of the A549-derived lung adenocarcinoma tumors growing in nude mice. A multiple case study
Autorzy:: Jakubowska, Monika
Śniegocka, Martyna
Podgórska, Ewa
Michalczyk-Wetula, Dominika
Urbanska, Krystyna
Susz, Anna
Fiedor, Leszek
Pyka, Janusz
Płonka, Przemysław
Powiązania:: https://bibliotekanauki.pl/articles/1039526.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: adenocarcinoma
histological analysis
metastases
nude mice
photodynamic therapy
zinc pheophorbide a
Opis:: Lung adenocarcinoma is a leading human malignancy with fatal prognosis. Ninety percent of the deaths, however, are caused by metastases. The model of subcutaneous tumor xenograft in nude mice was adopted to study the growth of control and photodynamically treated tumors derived from the human A549 lung adenocarcinoma cell line. As a side-result of the primary studies, observations on the metastasis of these tumors to the murine lungs were collected, and reported in the present paper. The metastasizing primary tumors were drained by a prominent number of lymphatic vessels. The metastatic tissue revealed the morphology of well-differentiated or trans-differentiated adenocarcinoma. Further histological and histochemical analyses demonstrated the presence of golden-brown granules in the metastatic tissue, similar to these found in the tumor tissue. In contrast to the primary tumors, the electron paramagnetic resonance spectroscopy revealed no nitric oxide - hemoglobin complexes (a source of intense paramagnetic signals), in the metastases. No metastases were found in other murine organs; however, white infarctions were identified in a single liver. Taken together, the A549-derived tumors growing subcutaneously in nude mice can metastasize and grow on site in the pulmonary tissue. Thus, they can represent an alternative for the model of induced metastatic nodule formation, following intravenous administration of the cancerous cells.
Źródło:: Acta Biochimica Polonica; 2013, 60, 3; 323-330
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Acute hepatologic and nephrologic effects of calcitriol in Syrian golden hamster (Mesocricetus auratus)
Autorzy:: Podgorska, Ewa
Sniegocka, Martyna
Mycinska, Marianna
Trybus, Wojciech
Trybus, Ewa
Kopacz-Bednarska, Anna
Wiechec, Olga
Krzykawska-Serda, Martyna
Elas, Martyna
Krol, Teodora
Urbanska, Krystyna
Slominski, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1038358.pdf
Data publikacji:: 2018
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: calcitriol
Syrian golden hamster
hepatologic toxicity
nephrologic toxicity
Opis:: Although vitamin D is included in the group of fat-soluble vitamins, it must be considered as a prohormone. Its active forms, including calcitriol, have pleiotropic effects and play an important role in the regulation of cell proliferation, differentiation and apoptosis, as well as in hormone secretion, and they demonstrate anti-cancer properties. Since calcitriol delivery can be beneficial for the organism, and Syrian golden hamsters represent a unique experimental model, we decided to investigate its toxicity in this species. In this study, we injected calcitriol intraperitoneally at doses 0 (control), 0.180±0.009 µg/kg and 0.717±0.032 µg/kg. Animal behavior was observed for 72 hrs after injection, and afterwards blood, liver and kidneys were collected for post-mortem examination, electron microscopy, and hematology analyses. The highest dose of calcitriol induced a change in animal behavior from calm to aggressive, and the liver surface showed morphological signs of damage. Following injection of calcitriol, ultrastructural changes were also observed in the liver and kidneys, e.g. vacuolization and increased number of mitochondria. There was also a trend for increased serum levels of aspartate aminotransferase (AST), but not of alanine aminotransferase (ALT) or GGTP (gamma-glutamyl transpeptidase). There was no change in Ca, Mg and P levels, as well as in blood morphology between experimental and control groups. These results indicate that calcitriol at 0.717, but not at 0.180 µg/kg, may induce acute damage to the liver and kidneys, without inducing calcemia. We propose that the hepatotoxic effect of calcitriol in hamster constitutes the primary cause of behavioral changes.
Źródło:: Acta Biochimica Polonica; 2018, 65, 3; 351-358
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Pokojowe misje ludowego Wojska Polskiego w świecie po 1945 roku : bibliografia
Autorzy:: Piwowarska, Krystyna.
Urbańska, Zofia.
Data publikacji:: 1996
Wydawca:: Warszawa : Wojskowa Akademia Polityczna
Tematy:: Misje pokojowe wojsko Polska 20 w.
Dostawca treści:: Bibliografia CBW

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Skocz do pozycji: 8.

Tytuł:: Pokojowe misje ludowego Wojska Polskiego w świecie po 1945 roku : bibliografia
Autorzy:: Piwowarska, Krystyna.
Urbańska, Zofia.
Powiązania:: Pokojowe misje ludowego Wojska Polskiego w świecie po 1945 roku : materiały z konferencji naukowej, Zegrze 20.10.1976 r Warszawa, 1977 S. 244-318
Data publikacji:: 1977
Tematy:: Misje pokojowe wojsko Polska 20 w.
Dostawca treści:: Bibliografia CBW