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    Wyświetlanie 1-8 z 8
    Tytuł:
    Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
    Autorzy:
    Drebert, Z.
    Golke, A.
    Cymerys, J.
    Slonska, A.
    Chmielewska, A.
    Tucholska, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30687.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Equid herpesvirus type 1 (EHV-1) is a prevalent causative agent of equine diseases worldwide. After primary replication in the respiratory epithelium the virus disseminates systemically through a peripheral blood mononuclear cell (PBMC)-associated viraemia. EHV-1 is the only alphaherpes-virus known so far which is capable of establishing latent infection not only in neurons but also in immune system cells (mainly in lymphocytes and macrophages). Since leukocytes are not the target cells for viral replication but are used to transport EHV-1 to the internal organs, the question remains how the virus avoids the immune response and whether it could potentially be associated with virus-induced cytoskeletal rearrangements. Therefore, the aim of this study was to investigate the progress of EHV-1 replication in leukocytes stimulated by phytohemagglutinin and the impact of EHV-1 infection on the actin cytoskeleton. Using the real-time PCR method we evaluated the quantity of viral DNA from samples collected at indicated time points post infection. In order to examine possible changes in actin cytoskeleton organization due to EHV-1 infection, we performed immunofluorescent staining using TRITC-phalloidin conjugate. The results showed that EHV-1 replicates in leukocytes at a restricted level but with the accompaniment of chromatin degradation. Simultaneously, infection with EHV-1 caused disruption of the actin cytoskeleton; this was particularly apparent in further stages of infection. Disruption of the actin cytoskeleton may lead to the limited release of the virus from the cells, but may be also beneficial for the virus, since at the same time it potentially impairs the immune function of leukocytes.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
    Autorzy:
    Golke, A.
    Cymerys, J.
    Slonska, A.
    Dzieciatkowski, T.
    Chmielewska, A.
    Tucholska, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30665.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    xCELLigence system
    real-time analysis
    label-free analysis
    neuronal cell response
    dermal cell response
    cell response
    equine herpesvirus 1
    animal infection
    herpesvirus
    neuron
    neuronal cell
    dermal cell
    Opis:
    Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line
    Autorzy:
    Cymerys, J.
    Slonska, A.
    Brzezicka, J.
    Tucholska, A.
    Chmielewska, A.
    Rola, J.
    Malik, P.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/32263.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
    Autorzy:
    Cymerys, J.
    Dzieciatkowski, T.
    Slonska, A.
    Bierla, J.
    Tucholska, A.
    Chmielewska, A.
    Golke, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31974.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    replication
    animal disease
    horse
    equine herpesvirus 1
    respiratory tract infection
    neurological disorder
    abortion
    neuron
    Opis:
    Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nieśmiałość a kompetencje temporalne młodzieży
    Shyness and temporal competence in youth
    Autorzy:
    Tucholska, Kinga
    Tucholska, Stanisława
    Powiązania:
    https://bibliotekanauki.pl/articles/2139065.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
    Tematy:
    shyness
    temporal competence
    Opis:
    The study refers to a problem of temporal competence in shy youth. The research question was: Whether and what differences in the level and the structure of temporal competences are in the group of young people with various level of shyness? The group of 286 (F–178, M–108) aged 15–18 years was tested. The Shyness Questionnaire by W. R. Crozier and The Temporal Competences Questionnaire by Z. Uchnast and K. Tucholska were applied. The analysis of results obtained allows to state that the shy youth has significantly lower level of temporal competence in general. They suffer from the lack of acceptance of their past and present situation, and deficit in prospective approach. The results of conducted research may direct the therapeutic work with shy people on the issues of their openness to various dimensions of psychological time. It should be noticed that the essence of shyness is not only in the lack of social or communicative competences but also it is connected with the lack of basic competences in psychological time experience.
    Źródło:
    Acta Universitatis Lodziensis. Folia Psychologica; 2011, 15; 45-64
    2353-4842
    Pojawia się w:
    Acta Universitatis Lodziensis. Folia Psychologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Poziom empatii a obraz siebie
    The Level of Empathy and Self-image
    Autorzy:
    Tucholska, Stanisława
    Powiązania:
    https://bibliotekanauki.pl/articles/1918804.pdf
    Data publikacji:
    2020-10-27
    Wydawca:
    Katolicki Uniwersytet Lubelski Jana Pawła II. Towarzystwo Naukowe KUL
    Opis:
    The object of the researches discussed in the present paper is a relation between the level of empathy and self-image in adolescent girls. The criterium of selecting experimental groups constituted the general findings of A. Mehrabian’s and W.A. Epstein’s Questionnaire of Empathy. Self-image was taken by means of H.G. Gough’s and A.B. Heilbrun’s ACL Test. The studies point that the differences among the persons from the experimental groups were statistically significant in the following scales: Int, Nur, Exh, Aut, Suc, Aba, Def, Cps, Fem, and FC. The persons with low empathy are self-assured, have a decreased ability for insight and are unwilling to establish closer emotional ties. In contacts with others they tend to expose themselves, to compete and accomplish their own aims, even at the cost of others. On the other hand the persons with high empathy easily establish closer contacts, are cooperation- oriented and mutual exchange; they do not tend to competition, are open in an unobtrusive manner; they respect their own and others’ individuality. The structure of self-image point to their good functioning in the relationship with other people. It is worth noting that thus empirically proved structure of needs and their personality correlates in individuals of different levels of empathy confirms the rightness of A. Mehrabian’s and W.A. Epstein’s questionnaire.
    Źródło:
    Roczniki Filozoficzne; 1994, 42, 4; 173-185
    0035-7685
    Pojawia się w:
    Roczniki Filozoficzne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stres i satysfakcja w zawodzie nauczyciela
    Stress and Satisfaction in a Teacher’s Job
    Autorzy:
    Tucholska, Stanisława
    Powiązania:
    https://bibliotekanauki.pl/articles/1857772.pdf
    Data publikacji:
    1999
    Wydawca:
    Katolicki Uniwersytet Lubelski Jana Pawła II. Towarzystwo Naukowe KUL
    Opis:
    The paper discusses the research on the level of stress and professional satisfaction in teachers. 747 working teachers from primary schools have been examined (611 women and 136men). The studies were conducted with the use of the Questionnaire of Teacher's Stress constructed by the author. The findings point that for ca. 23% of teachers their job is stressful or very stressful, for 63% is stressful on average, and 13% of teachers do not feel like being under stress at all. The majority of teachers under study (63%) decidedly like their job and feel satisfaction (57%). Among the subjects, however, there are such teachers who do not like their job (9%) and do not feel satisfied (13%). As regards salaries, the teachers' opinion is very negative: 81% think that financial gratification is too low, and only 19% say that it is satisfactory.
    Źródło:
    Roczniki Nauk Społecznych; 1999, 27, 2; 51-61
    0137-4176
    Pojawia się w:
    Roczniki Nauk Społecznych
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Poczucie koherencji a zachowania zaradcze młodzieży
    Sense of coherence and remedial behaviours of the young
    Autorzy:
    Płaczkiewicz, Beata
    Tucholska, Stanisława
    Powiązania:
    https://bibliotekanauki.pl/articles/2087375.pdf
    Data publikacji:
    2009
    Wydawca:
    Uniwersytet Kardynała Stefana Wyszyńskiego w Warszawie
    Opis:
    The researches reported in this paper seek the relationship between sense of coherence and coping styles. The studies embraced a group of 192 high-school adolescents (132 girls and 60 boys) aged between 17 and 18 years. Two methods have been used in this research: Antonovsky’s The Sense of Coherence Questionnaire (SOC-29) and Coping Inventory for Stressful Situations (CISS) authored by Endler and Parker. In addition cluster analysis carried on the results in scales and subscales of CISS Questionnaire resulted into three group of pupils. The groups were compared in respect of sense of coherence. An analysis of the obtained results showed a clear correlation between coping and the sense of coherence in all population of researched adolescents. There is a positive interrelation between Task-oriented coping and global sense of coherence and its components- the sense of comprehensibility, manageability and meaningfulness. Moreover, there were found significant correlations of SOC and its components with Emotion- oriented coping, but this time they appeared negative. Furthermore, there isnt relation between SOC and Avoidance- oriented coping associated with bewaring of thinking and experiencing stressful situations. What is interesting Distraction and Social Diversion which are subscales of Avoidance-oriented coping, correlate with SOC.  
    Źródło:
    Studia Psychologica; 2009, 9; 45-56
    1642-2473
    Pojawia się w:
    Studia Psychologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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