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    Wyświetlanie 1-2 z 2
    Tytuł:
    Amoebicidal or amoebostatic influence of disinfectants used in health facilities and laboratories on corneal strains of Acanthamoeba
    Autorzy:
    Baltaza, Wanda
    Padzik, Marcin
    Szaflik, Jacek P.
    Dybicz, Monika
    Hendiger, Edyta
    Chomicz, Lidia
    Powiązania:
    https://bibliotekanauki.pl/articles/972267.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    in vitro dynamics
    influence of disinfectants
    Acanthamoeba strains
    Opis:
    Different Acanthamoeba species are amphizoic organisms distributed in wide range of habitats in natural and man-made environments; they are also detected on surfaces of equipment and accessories in health facilities. Some strains of the amoebae are causative agents of the vision-threatening human disease Acanthamoeba keratitis, mainly reported in contact lens wearers. An exceptional high resistance of Acanthamoeba trophozoites and particularly cysts to chemicals, disinfectants and drugs is believed as influencing difficulty resulting in unsuccessful therapeutic management. As Acanthamoeba keratitis is the serious medical problem worldwide, different chemicals with possible activity against environmental and clinical Acanthamoeba strains are tested. In our study, selected disinfectants used in health care settings and laboratories were tested and their efficacy against the corneal strains Acanthamoeba castellanii and A. polyphaga , and environmental A. castellanii Neff strain was assessed. Comparative assessment of results of the assays show that, apart from amoebistatic effects, the disinfectants indicated expected cysticidal efficacy.
    Źródło:
    Annals of Parasitology; 2017, 63, 3; 162-172
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vivo confocal microscopy and in vitro culture techniques as tools for evaluation of severe Acanthamoeba keratitis incidents
    Autorzy:
    Padzik, Monika
    Szaflik, Jacek P.
    Baltaza, Wanda
    Perkowski, Konrad
    Dybycz, Monika
    Chomicz, Lidia
    Powiązania:
    https://bibliotekanauki.pl/articles/972158.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Acanthamoeba keratitis
    in vitro cultivation
    in vivo confocal microscopy
    Opis:
    Amphizoic amoebae belonging to the genus Acanthamoeba are known as etiological agents of sightthreatening Acanthamoeba keratitis. The leading risk factor for the development of this serious human disease is contact lens wearing which popularity increases worldwide, also in Poland. The disease with active epithelial inflammations, corneal ulcers, including loss of the visual acuity is a serious medical problem as an emerging threat for the public health related to improper contact lens hygiene. The treatment of the amoebic keratitis is difficult, often unsuccessful due to delayed proper diagnosis. The clinical picture of the disease, often with severe course is nonspecific, similar to that occurring in viral, fungal or bacterial keratitis, thus clinical symptoms alone are not sufficient to identify the causative agent of the amoebic infection. Early diagnosis is decisive for the suitable therapeutic management and the treatment efficacy. In our studies, several complicated, difficult to treat Acanthamoeba keratitis incidences pertaining Polish patients using contact lenses have been retrospectively analyzed in terms of the usefulness of non-invasive methods of in vivo confocal microscopy and in vitro culture techniques applied for diagnosis. Hyper-reflective double-walled spherical Acanthamoeba cysts, with a more reflective outer wall were detected in the epithelium and anterior layers of the corneal stroma. In vivo confocal microscopy, if available, may be a valuable, sensitive tool for diagnosis in late identified severe infections mainly with strong viability strains, however confoscan may offer limited value at lowintensity amoebic infections. The microscopic visualization of amoebae in slides prepared directly from corneal scraping and laboratory examinations of specimens from in vitro cultivated corneal isolates allow to confirm or verify results of in vivo examinations, furthermore to identify directly the pathogens and to clarify previous misdiagnoses.
    Źródło:
    Annals of Parasitology; 2017, 63, 4; 341-346
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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