- Tytuł:
- Evaluation of P1 substrate specificity of staphylococcal SplB protease
- Autorzy:
-
Pustelny, Katarzyna
Stach, Natalia
Wladyka, Benedykt
Dubin, Adam
Dubin, Grzegorz - Powiązania:
- https://bibliotekanauki.pl/articles/1039355.pdf
- Data publikacji:
- 2014
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
serine protease
serine protease-like
SplB
Staphylococcus aureus
substrate specificity - Opis:
- Staphylococcus aureus is a dangerous human pathogen characterized by growing antibiotic resistance. Virulence of S. aureus relies on a variety of secreted and cell surface associated virulence factors among which certain proteolytic enzymes play an important role. Amid staphylococcal extracellular proteases, those encoded by the spl operon remain poorly characterized, both in terms of enzymology and their physiological role. Initial data demonstrated that Spl proteases exhibit restricted substrate specificity. This study describes development of convenient protein FRET substrates for SplB protease and characterization of the substrate preference of the protease at the P1' position. Kinetic data on hydrolysis of a panel of substrates substituted at the said position is provided.
- Źródło:
-
Acta Biochimica Polonica; 2014, 61, 1; 149-152
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł