- Tytuł:
- Usefulness of real-time PCR in long-term follow-up of follicular lymphoma patients
- Autorzy:
-
Tysarowski, Andrzej
Fabisiewicz, Anna
Paszkiewicz-Kozik, Ewa
Kulik, Jadwiga
Walewski, Jan
Siedlecki, Janusz - Powiązania:
- https://bibliotekanauki.pl/articles/1041125.pdf
- Data publikacji:
- 2007
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
real-time PCR
follicular lymphoma
minimal residual disease
molecular remission - Opis:
- The aim of this study was to evaluate the usefulness of quantitative real-time PCR (RQ-PCR) for the monitoring of molecular remission in follicular lymphoma (FL) patients during long-term follow-up. RQ-PCR by the use of TaqMan® detection system is a sensitive tool to monitor minimal residual disease (MRD) in FL through amplification of the t(14;18) fusion gene during and post-therapy. In most cases the breakpoint region occurs within the major breakpoint region (MBR). Among 75 patients diagnosed with FL, cells harboring the fusion gene BCL2/JH were found in peripheral blood of 31 patients (41%). We further monitored 30 of these patients in a period varying from 6 months to 5 years by RQ-PCR. In our study the level indicating the possibility of the presence of MRD was established at more than five t(14;18)-positive cells in the background of 83000 normal cells. The results of this work also confirmed that the presence of MRD detected by RQ-PCR is an indication for careful observation of patients because of a higher risk of disease recurrence.
- Źródło:
-
Acta Biochimica Polonica; 2007, 54, 1; 135-142
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł