- Tytuł:
- Assessment of 8-oxo-7,8-dihydro-2′-deoxyguanosine as a marker of oxidative DNA damage in gasoline filling station attendants
- Autorzy:
-
Beerappa, Ravichandran
Venugopal, Dhananjayan
Sen, Somnath
Ambikapathy, Mala
Rao, Rajmohan H. - Powiązania:
- https://bibliotekanauki.pl/articles/2179055.pdf
- Data publikacji:
- 2013-10-01
- Wydawca:
- Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
- Tematy:
-
DNA damage
ELISA
petrol filling attendants
urinary 8-oxo-7
8-dihydro-2’-deoxyguanosine - Opis:
- Objectives: The urinary excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) was used as a biomarker of oxidative DNA damage. The urinary 8-oxodG levels in petrol filling station attendants (exposed) at various petrol bunks were estimated as well as in the unexposed (cashier) population. Materials and Methods: A total of 100 workers (79 petrol fillers and 21 cashiers) aged from 20 to 41 years participated in the study. An informed consent was taken from each participant. Information on personal habits and health was obtained through a questionnaire. After shifts, urine samples were collected analyzed for 8-oxodG using enzyme-linked immunosorbent assay (ELISA). Results: Fifty-three percent of workers were in the 21-30 years age group. The maximum level of 8-oxodG was observed in the age group ≥ 41 years and the minimum in the age group of 31-40 years. The maximum level of 8-oxodG was observed among those workers who had ≥ 21 years of experience. The concentrations of 8-oxodG were significantly higher in petrol fillers than those in cashiers (p < 0.05). Conclusions: Despite the conflicting results obtained in our study it was shown that 8-oxodG is related to chemical exposure. Further research is needed embracing a bigger number of participants to highlight the correlations between the exposure and the effects.
- Źródło:
-
International Journal of Occupational Medicine and Environmental Health; 2013, 26, 5; 780-789
1232-1087
1896-494X - Pojawia się w:
- International Journal of Occupational Medicine and Environmental Health
- Dostawca treści:
- Biblioteka Nauki
Artykuł