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Wyświetlanie 1-2 z 2
Tytuł:
Biomolecular characterization of Fusarium poae strains isolated from durum wheat in central Italy.
Autorzy:
Alberti, I.
Prà, Dal M.
Tonti, S.
Montanari, M.
Prodi, A.
Pancaldi, D.
Powiązania:
https://bibliotekanauki.pl/articles/2199628.pdf
Data publikacji:
2011-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
durum wheat
FHB
F. poae
gene sequencing
mycotoxins
trichothecenes
Opis:
Fusarium Head Blight (FHB) is a worldwide disease affecting wheat, barley and other grains, reducing kernel weight and grain yield; infected seeds may contain a large number of mycotoxins, including trichothe- cenes of type A and B. These compounds have already been associated with human and animal toxicoses. Most common species causing the disease are F. graminearum, F. culmorum and F. avenaceum, but in the last few years a gradual increase in incidence of another species, F. poae, has been reported. In general terms, F. poae is a relatively weak pathogen, but its contribute to the increase of mycotoxins level still has to be clarified. Durum wheat is widely cultivated in the central part of Italy, however the effective incidence of F. poae in this area still has to be investigated. In order to monitor Fusarium risk, we collected dozens of F. poae strains on seeds and glumes of durum wheat coming from some of the most important cultivated areas of Central Italy. Every isolate was identified both by microscope observation and by PCR assay with the primer pair Fp82 F/R. Strains were therefore subjected to a more accurate molecular characterization by Translation Elongation Factor 1-alpha (TEF-1α) gene sequencing.  
Źródło:
Plant Breeding and Seed Science; 2011, 64; 153-161
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions.
Autorzy:
Prodi, A.
Salomoni, D.
Bertacchini, E.
Alkadri, D.
Pisi, A.
Tonti, S.
Alberti, I.
Dal Prà, M.
Pancaldi, D.
Covarelli, L.
Nipoti, P.
Powiązania:
https://bibliotekanauki.pl/articles/2199616.pdf
Data publikacji:
2011-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
DON and NIV chemotypes
durum wheat
Fusarium Head Blight
Tri12
Opis:
Durum wheat production in Italy is economically of great importance. Fusarium graminearum is the main fusarium head blight (FHB) causal agent in wheat, reducing both yield and grain quality. F. graminearum produces several mycotoxins and, among trichothecenes, deoxynivalenol (DON) and nivalenol (NIV) are the most studied for their toxicity towards humans and animals. DON-producing isolates can be further distin- guished on the basis of the predominant acetyl-DON derivative in 3-acetyldeoxynivalenol (3-ADON) or 15- acetyldeoxynivalenol (15-ADON). In order to evaluate possible mycotoxin contamination risks in food, it is very important to know which chemotype is the prevalent in a F. graminearum population. F. graminearum sensu stricto strains were collected from symptomatic durum wheat heads and grains of several naturally infected fields located mostly in Emilia – Romagna, The Marche, Lazio, Tuscany and Umbria. A multiplex PCR in the region of genes Tri12, located in the terminal gene cluster of trichothecenes, was used to characterize 187 single-spore isolates of F. graminearum as NIV, 3-ADON and 15-ADON chemotypes. All the three chemotypes were present in the F. graminearum population studied. The most frequent chemotype was 15-ADON (83.4%), followed by 3-ADON (10.7%) and NIV (5.9%). NIV-producing isolates were found only in Emilia-Romagna (3.5%), Umbria (33.3%) and The Marche (5.7%).
Źródło:
Plant Breeding and Seed Science; 2011, 64; 75-80
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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