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Wyszukujesz frazę "Mazurkiewicz, Alina" wg kryterium: Wszystkie pola

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    Wyświetlanie 1-3 z 3
    Tytuł:
    Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system
    Autorzy:
    Mazurkiewicz-Pisarek, Anna
    Mazurkiewicz, Alina
    Mikiewicz, Diana
    Baran, Piotr
    Ciach, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/16706242.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    factor VIII
    hemophilia type A
    recombinant coagulation factor VIII
    prokaryotic expression system
    E. coli
    recombinant protein production system
    Opis:
    In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of large quantities of recombinant proteins in a short time. With this study, we aimed to obtain recombinant blood coagulation factor VIII using the E. coli bacterial expression system, a method not previously explored for this purpose. Our research encompasses the synthesis of blood coagulation factor VIII and its expression in a prokaryotic system. To achieve this, we constructed a prokaryotic expression vector containing a synthetic factor VIII gene, which was then used for the transformation of an E. coli bacterial strain. The protein expression was confirmed by mass spectrometry, and we assessed the stability of the gene construct while determining the optimal growth conditions. The production of blood coagulation factor VIII by the E. coli bacterial strain was carried out on a quarter-technical scale. We established the conditions for isolation, denaturation, and renaturation of the protein, and subsequently confirmed the activity of FVIII.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 3; 247-262
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reduction of Greenhouse Gas Emissions by Replacing Fertilizers with Digestate
    Autorzy:
    Kowalczyk-Juśko, Alina
    Pochwatka, Patrycja
    Mazurkiewicz, Jakub
    Pulka, Jakub
    Kępowicz, Barbara
    Janczak, Damian
    Dach, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/24201726.pdf
    Data publikacji:
    2023
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    biogas plant
    anaerobic digestion
    digestate
    fertilizing
    emission reduction
    Opis:
    Digestate from a biogas plant can be a valuable organic and mineral fertilizer. Quantitative proportions of cosubstrates used in three agricultural biogas plants in Poland were analyzed. The composition of digestates was examined and large differences in the content of macronutrients were found, especially N and K. On the basis of the factors used to calculate emissions from the production and use of artificial fertilizers, the greenhouse gas (GHG) reduction resulting from replacing mineral fertilizers with digestate was calculated. In terms of 1 Mg of fresh digestate, this reduction may not seem large, as it amounts to 27.9–61.6 kg of CO2 eq, but it should be taken into account that digestate contains little dry matter. The annual amount of digestate used on an area of 1 ha allows avoiding GHG emissions of 25.8–44.5 Mg CO2 eq.
    Źródło:
    Journal of Ecological Engineering; 2023, 24, 4; 312--319
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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