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Wyświetlanie 1-4 z 4
Tytuł:
Variability of the specific fluorescence of chlorophyll in the ocean. Part 2. Fluorometric method of chlorophyll a determination
Autorzy:
Ostrowska, M.
Matorin, D.N.
Ficek, D.
Powiązania:
https://bibliotekanauki.pl/articles/47939.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
variability
luminescence
determination
chlorophyll a
fluorescence
sea
fluorometric method
phytoplankton
ocean
chlorophyll
Opis:
Two methods of determining the chlorophyll a concentration in the sea have been formulated on the basis of artificially induced fluorescence measured with the aid of submersible fluorometers. The method of statistical correlation is founded on the empirical relationship between fluorescence and chlorophyll concentration. The theoretical model of fluorescence described in Part 1 of this paper (see Ostrowska et al. 2000, this volume) provides the basis of the other method, the physical method. This describes the dependence of the specific fluorescence of phytoplankton on the chlorophyll concentration, a diversity of photophysiological properties of phytoplankton and the optical characteristics of the fluorometer. In order to assess their practicability, the methods were subjected to empirical verification. This showed that the physical method yielded chlorophyll concentrations of far greater accuracy. The respective error factors of the estimated chlorophyll concentration were x = 2.07 for the correlation method and x = 1.5 for the physical method. This means that the statistical logarithmic error varies from −52 to +107% in the case of the former method but only from −33 to +51% in the case of the latter. Thus, modifying the methodology has much improved the accuracy of chlorophyll determinations.
Źródło:
Oceanologia; 2000, 42, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Variability of the specific fluorescence of chlorophyll in the ocean. Part 1. Theory of classical in situ chlorophyll fluorometry
Autorzy:
Ostrowska, M.
Majchrowski, R.
Matorin, D.N.
Wozniak, B.
Powiązania:
https://bibliotekanauki.pl/articles/48349.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
marine phytoplankton
variability
luminescence
in situ
fluorescence
depth
sea
fluorometric method
phytoplankton
ocean
chlorophyll
light absorption
Opis:
The range of variability of the fluorescence properties of marine phytoplankton in different trophic types of seas and at different depths in the sea is analysed theoretically. An attempt is also made to interpret artificially induced in situ fluorescence measured with submersible fluorometers. To do this, earlier optical models of light absorption by phytoplankton (see Woźniak et al. 2000, this volume) and actual empirical data were applied. A straightforward theoretical model of artificially photoinduced phytoplankton fluorescence accounting for the complex influence of different photophysiological characteristics of phytoplankton and the optical characteristics of the instrument has been worked out. A physical method of determining chlorophyll a concentrations in seawater from fluorescence measured in situ with contact fluorometers can be based on this model.
Źródło:
Oceanologia; 2000, 42, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chlorophyll fluorimetry as a method for studying light absorption by photosynthetic pigments in marine algae
Autorzy:
Matorin, D.N.
Antal, T.K.
Ostrowska, M.
Rubin, A.B.
Ficek, D.
Majchrowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/48459.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
alga
chlorophyll a concentration
photosynthetic pigment
fluorescence
marine alga
phytoplankton
light absorption
chlorophyll fluorimenty
Opis:
Using laboratory cultures of algae and natural phytoplankton populations from Nhatrang Bay (South China Sea), the relationship between the chlorophyll fluorescence F0, the chlorophyll a concentration Ca and light absorption capacities of algae cells was studied. It is shown that the ratio F0/Ca depends mainly on the species composition of the algae population; hence, the concentration Ca can be measured with the fluorescence method with acceptable accuracy only when the species composition of algae populations varies over a rather narrow range. The fluorescence F0 can, however, be a good index of the total absorption capacities of different phytoplankton species, because the intensity of F0 depends on the sum total of light absorbed by all photosynthetic pigments in a plant cell. Thus, the fluorescence F0 measures not only the concentration of chlorophyll a, but that of all photosynthetic pigment concentrations.
Źródło:
Oceanologia; 2004, 46, 4
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Measurement of phytoplankton photosynthesis rate using a pump-and-probe fluorometer
Autorzy:
Antal, T.K.
Venediktov, P.S.
Matorin, D.N.
Ostrowska, M.
Wozniak, B.
Rubin, A.B.
Powiązania:
https://bibliotekanauki.pl/articles/47511.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
phytoplankton
photosynthesis rate
photosynthesis measurement
fluorometer
plant luminescence
fluorometric method
primary production
Opis:
In this work we have studied the possibility of determining the rate of phytoplankton photosynthesis in situ using a submersible pump-and-probe fluorometer in water areas differing in their trophic level, as well as in climatic and hydrophysical characteristics. A biophysical model was used to describe the relationship between photosynthesis, underwater irradiance, and the intensity of phytoplankton fluorescence excited by an artificial light source. Fluorescence intensity was used as a measure of light absorption by phytoplankton and for assessing the efficiency of photochemical energy conversion at photosynthetic reaction centers. Parameters of the model that could not be measured experimentally were determined by calibrating fluorescence and irradiance data against the primary production measured in the Baltic Sea with the radioactive carbon method. It was shown that the standard deviation of these parameters in situ did not exceed 20%, and the use of their mean values to estimate the phytoplankton photosynthetic rate showed a good correlation between the calculated and measured data on primary production in the Baltic (r = 0.89), Norwegian (r = 0.77) and South China (r = 0.76) Seas.
Źródło:
Oceanologia; 2001, 43, 3; 291-313
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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