Wszystkie pola: MCF-7 cell line - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Synthesis and in vitro anticancer activity of N-alkyl phosphoramidate monoesters of 3N-azido-3N-deoxythymidine (AZT)
Autorzy:: Czajkowska-Wojciechowska, E.
Singh, A.
Trznadel, R.
Ruszkowski, P.
Celewicz, L.
Powiązania:: https://bibliotekanauki.pl/articles/81295.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: anticancer activity
N-alkyl phosphoramidate monoester
3N-azido-3N-deoxythymidine
human cell line
cancer cell line
cervical cancer
HeLa cell line
KB cell
MCF-7 cell line
dermal fibroblast
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 2.

Tytuł:: The effect of Topotecan on oxidative stress in MCF-7 human breast cancer cell line
Autorzy:: Timur, Mujgan
Akbas, S
Ozben, Tomris
Powiązania:: https://bibliotekanauki.pl/articles/1041340.pdf
Data publikacji:: 2005
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: MCF-7 cells
Topotecan
antioxidants
breast cancer
oxidative stress
Opis:: Purpose. Topotecan, a semisynthetic water-soluble derivative of camptothecin exerts its cytotoxic effect by inhibiting topoisomerase I and causes double-strand DNA breaks which inhibit DNA function and ultimately lead to cell death. In previous studies it was shown that camptothecin causes ROS formation. The aim of this study was to investigate if Topotecan like camptotecin causes oxidative stress in MCF-7 human breast cancer cell line. Determining the oxidant effect of Topotecan may elucidate a possible alternative mechanism for its cytotoxicity. Experimental design. MCF-7 cells were cultured and exposed to Topotecan for 24 h at 37°C. The viability of the cells (% of control) was measured using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Lipid peroxidation (TBARS), protein oxidation (carbonyl content), sulfhydryl, glutathione (GSH) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were determined in MCF-7 cells with and without Topotecan incubation. Results. We found the IC50 concentration of Topotecan as 0.218 µM in MCF-7 cells. This concentration of Topotecan was used in the incubations of the cells. Our data indicated increased oxidative status, as revealed by increased lipid peroxidation and protein oxidation, and decreased GSH and sulfhydryl levels in MCF-7 cells exposed to Topotecan compared to control cells. In contrast, there was a slight increase in SOD and a significant increase in GPx and catalase activity in MCF-7 cells incubated with Topotecan compared to the control. Conclusions. These results support our hypothesis that Topotecan increases oxidative stress in MCF-7 cells.
Źródło:: Acta Biochimica Polonica; 2005, 52, 4; 897-902
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Informacja

Wyszukujesz frazę "MCF-7 cell line" wg kryterium: Wszystkie pola

Źródło danych

Dostawca treści

Kolekcja

Rok wydania

Wydawca

Temat

Autor

Typ dokumentu

Język