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Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
Autorzy:
Shen, L.
Qian, B.
Xiao, J.
Zhu, Y.
Hussain, S.
Deng, J.
Peng, G.
Zuo, Z.
Zou, L.
Yu, S.
Ma, X.
Zhong, Z.
Ren, Z.
Wang, Y.
Liu, H.
Zhou, Z.
Cai, D.
Hu, Y.
Zong, X.
Cao, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087289.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adiponectin
dairy cows
ketosis
leptin
perinatal period
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties
Autorzy:
Feng, X.
Xu, Y.
Liu, D.
Peng, L.
Dong, J.
Yao, S.
Feng, Y.
Feng, Z.
Li, F.
Hu, B.
Powiązania:
https://bibliotekanauki.pl/articles/12703546.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2020, 19, 1; 71-84
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New data on the anatomy of fuxianhuiid arthropod Guangweicaris spinatus from the lower Cambrian Guanshan Biota, Yunnan, China
Autorzy:
Chen, H.
Legg, D.A.
Zhai, D.-Y.
Liu, Y.
Hou, X.-G.
Powiązania:
https://bibliotekanauki.pl/articles/2082130.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
Arthropoda
Euarthropoda
Fuxianhuiida
Fuxianhuiidae
Guanshan Biota
Cambrian
Asia
Opis:
The fuxianhuiid arthropod Guangweicaris spinatus, from the lower Cambrian (Series 2, Stage 4), Guanshan Biota (Wulongqing Member, Canglangpu Formation), located in the vicinity of Kunming (Yunnan Province, southwest China), is redescribed based on new specimens and a re-examination of previously described material. A more complete overview of its morphology is given. Newly recognised features include: (i) a medial cephalic bulge; (ii) a tripartite hypostome; (iii) a pair of specialized post-antennal appendage (SPA); (iv) a putative telson; (v) two pairs of spines on the posteroventral margin of the terminal abdominal segment. This information is used to provide an emended diagnosis of the family Fuxianhuiidae, and the genus Guangweicaris.
Źródło:
Acta Palaeontologica Polonica; 2020, 65, 1; 139-148
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of dry matter translocation and grain yield of summer maize to biodegradable film in the North China Plain
Autorzy:
Tan, D.
Guo, L.
Liu, J.
Fan, Y.
Li, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2082862.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
polyethylene film
leaf area index
dry matter
transfer
yield components
North China Plain
Opis:
In the North China Plain, it is a matter of urgency to explore the feasibility of using biodegradable film to replace polyethylene film. A field experiment was conducted by covering soils with polyethylene white film, biodegradable white film, biodegradable black film, while the control remained uncovered. This study analysed the effects of using different film types on summer maize dry matter accumulation and transfer, grain yield and yield components during the 2016 and 2017 summer maize growing seasons. Results showed that, for both growing seasons, compared with non-mulching, dry matter translocation, dry matter transfer efficiency of vegetative organs and grain yield for plants following polyethylene white film and biodegradable white film treatments were always lower. However, dry matter accumulation, dry matter translocation, dry matter transfer efficiency, grain yield, and the contribution of dry matter translocation to grain yield before flowering in biodegradable black film treatments significantly increased by 21.0, 33.3, 21.4, 12.6, and 18.5%, respectively. Only the black biodegradable film could increase grain yield as determined by the 1000 kernel mass. Results indicate that black biodegradable films are a viable alternative to polyethylene film in summer maize production in the North China Plain.
Źródło:
International Agrophysics; 2020, 34, 1; 87-94
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria
Autorzy:
Pei, Z.
Ying, X.
Tang, Y.
Liu, L.
Zhang, H.
Liu, S.
Zhang, D.
Wang, K.
Kong, L.
Gao, Y.
Ma, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087656.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antimicrobial peptide
biological characteristics
antibacterial mechanism
Gram-negative bacteria
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 533-542
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Common mycelium networks with Paraglomus occultum induce better plant growth and signal substance changes between trifoliate orange seedlings
Autorzy:
Zhang, Y.-C.
Xie, M.-M.
Feng, H.-D.
Zhou, M.
Zhang, Z.Z.
Liu, C.-Y.
Wu, Q.-S.
Powiązania:
https://bibliotekanauki.pl/articles/11877054.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
Mycorrhizal mycelium can simultaneously colonize two and more neighboring plants to form common mycelium network (CMNs), whereas the information regarding CMN effects on endogenous signal substances is limited. In this study, a rootbox was separated by 37- or 0.45-μm mesh to establish donor chamber (the presence of roots and hyphae) and receptor (hyphae presented or not, free of roots) chamber, where an arbuscular mycorrhizal (AM) fungus Paraglomus occultum was inoculated into trifoliate orange seedlings of donor chamber to illustrate the underground communications of signal substances by CMNs. Mycorrhizal colonization resulted in better plant growth performance and greater root morphology in donor and receptor plants. AM inoculation increased significantly the root nitric oxide (NO) and calmodulin (CaM) levels of donor plants, regardless of 37- and 0.45-μm mesh, and subsequent CMNs induced higher root NO and CaM levels in receptor plants. Mycorrhizal colonization did not produce significant changes in root zeatin riboside (ZR) levels of donor plants, but CMN hyphae modulated lower root ZR levels of receptor plants. Mycorrhizal inoculation and subsequent CMN hyphae induced lower root gibberellin levels of donor and receptor plants, and only CMN hyphae produced lower root methyl jasmonate concentrations of receptor plants. Our results first reveal the underground communication of CaM, NO, and ZR by CMNs with P. occultum between trifoliate orange seedlings.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 6; 95-104
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Copper adsorption reaction rate and ion exchange ratio during the copper activation of sphalerite
Autorzy:
Liu, J.
Luo, D.
Huang, L.
Wang, Y.
Wen, S.
Powiązania:
https://bibliotekanauki.pl/articles/110651.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
sphalerite
copper activation
solution chemistry
ion exchange ratio
reaction rate
Opis:
A theoretical analysis method of the solution chemistry characteristic of Cu(II) at various pH values was developed. Using such method, the existence form of Cu species and their proportions in aqueous solution can be clearly determined. After that, the copper adsorption and ion exchange ratio (E) during the copper activation of sphalerite were investigated. The results indicated that the copper activation of sphalerite can be divided into two stages, i.e., the fast activation stage (t ≤4 min) and slow activation stage (t >4 min). The general form of the copper adsorption rate was determined as ΓCu=K1ln(t) +Γ1, which is confirmed by the data fitting of the fast activation stage. The lower activation pH results in higher ion exchange efficiency for Cu substituting Zn. For a strong acid pH of 4.1, the E maintains about 1:1 in the entire activation time range and is not dependent on the activation time. However, the value of E is greater than 1 under weak acidic (pH=6.2) and alkaline (pH=9.1) pH conditions and it significantly depends on the activation time. For such conditions, E decreases with increase in the activation time, which supports the ion exchange mechanism, but within short activation time, it is not a 1:1 ion exchange process.
Źródło:
Physicochemical Problems of Mineral Processing; 2018, 54, 2; 377-385
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of Polymeric Binders on the RDX-based Explosive Response Character under Slow Cook-off Conditions
Autorzy:
Yan, X.
Li, X. D.
Zhang, Y. R.
Liu, L.
Zhang, X. M.
Tan, Y. X.
Wang, H.
Wang, X. Q.
Powiązania:
https://bibliotekanauki.pl/articles/358419.pdf
Data publikacji:
2018
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
RDX-based explosive
slow cook-off experiment
binder content
binder type
Opis:
Due to safety requirements, insensitive behaviour under slow thermal heating (cook-off) conditions is a desirable behaviour for today’s munitions. In this paper a cook-off device is designed to test two groups of RDX-based PBX explosives. In the first group the binder type was varied and in the second group the binder content of the RDX-based explosive was changed. Eleven samples were examined in order to evaluate the influence of four different binders and seven different binder contents on the shell deformation and the degree of the involved reaction. The test results showed that the degree of the reaction can be improved by changing the binder content, but not by the binder type. This phenomenon was explained by the thermal-conduction theory.
Źródło:
Central European Journal of Energetic Materials; 2018, 15, 2; 339-350
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preparation and cytological study of collagen/nano-hydroxyapatite/graphene oxide composites
Autorzy:
Wang, J.
Wang, Y.
Liu, D.
Yang, Q.
Huang, C.
Yang, C.
Zhang, Q.
Powiązania:
https://bibliotekanauki.pl/articles/307450.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
nanohydroksyapatyt
tlenek grafitu
mineralizacja
kolagen
nano-hydroxyapatite
graphene oxide
biomimetic mineralization
collagen
Opis:
Biomimetic mineralized composite scaffolds are widely used as natural bone substitute materials in tissue engineering by inducing and assembling bonelike apatite. In this study, the single lamellar structure of graphene oxide (GO) powder was prepared via an improved Hummers’ method. Methods: To better mimic natural bone, the collagen (COL)/Nano-hydroxyapatite (nHA)/graphene oxide (GO) composite material was prepared by simulated body fluid (SBF) method using COL/GO as a matrix template. Hydroxyapatite (HA) with calcium ion deficiency was achieved via biomimetic mineralization, and it had properties closer to those of natural bone than pure HA has. Results: The mineralized COL/nHA/GO composites exhibited loose porous structures, and the connectivity of the holes was good and thus beneficial to the exchange of nutrients and excreted metabolites. Conculsions: Antibacterial and MTT experiment confirmed that the COL/nHA/GO composite material had excellent antibacterial property and biocompatibility. Hence, these results strongly suggested the mineralized COL/nHA/GO composite is a good candidate biomaterial to be applied in bone tissue engineering.
Źródło:
Acta of Bioengineering and Biomechanics; 2018, 20, 4; 65-74
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Propagation properties of anomalous hollow vortex beam in uniaxial crystal orthogonal to the optical axis
Autorzy:
Liu, D.
Wang, Y.
Wang, G.
Powiązania:
https://bibliotekanauki.pl/articles/174466.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
uniaxial crystal
anomalous hollow vortex beam
laser propagation
Opis:
The analytical equation of an anomalous hollow vortex beam propagating in a uniaxial crystal orthogonal to the optical axis is derived, and the intensity and phase properties of this beam propagating in the uniaxial crystal are illustrated using numerical examples. The influences of topological charge M and the ratio of refractive index ne/no of a uniaxial crystal on the normalized intensity distribution and phase distribution for anomalous hollow vortex beam are discussed and analyzed in detail.
Źródło:
Optica Applicata; 2018, 48, 1; 95-104
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study of the force and deformation characteristics of subsea mudmat-pile hybrid foundations
Autorzy:
Kong, D.
Deng, M.
Liu, Y.
Tan, X.
Powiązania:
https://bibliotekanauki.pl/articles/258965.pdf
Data publikacji:
2018
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
subsea mudmat-pile hybrid foundations
force characteristics
deformation characteristics
combined loads
Opis:
To study the force and deformation characteristics of subsea mudmat-pile hybrid foundations under different combined loads, a project at a water depth of 200 m in the South China Sea was studied. A numerical model of a subsea mudmatpile hybrid foundation is developed using the numerical simulation software FLAC3D. The settlement of the seabed soil, the bending moments of the mudmat, and the displacements and bending moments along the pile shaft under different load combinations, including vertical load and horizontal load, vertical load and bending moment, and horizontal load and bending moment load, are analyzed. The results indicate that settlement of the seabed soil is reduced by the presence of piles. The settlement of the mudmat is reduced by the presence of piles. Different degrees of inclination occur along the pile shaft. The angle of inclination of pile No. 1 is greater than that of pile No. 2. The dip directions of piles No. 1 and No. 2 are identical under the vertical load and bending moment and are opposite to those under the other combined loads. The piles that are located at the junctions between the mudmat and the tops of the piles are easily destroyed.
Źródło:
Polish Maritime Research; 2018, S 3; 43-53
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
UV-Vis spectroscopic detection coupled with chemometrics for the measurement of mixed organic acids in water samples enriched by radial electric focusing solid phase extraction
Autorzy:
Guo, Y.
Liu, X.
Liu, J.
Bian, X.
Zhang, Q.
Pan, J.
Wan, D.
Powiązania:
https://bibliotekanauki.pl/articles/221808.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chemometrics
UV-Vis spectrophotometer
radial electric focusing solid phase extraction
mixed organic acids
Opis:
Due to the difficulty of detecting traces of organic acid mixture in an aqueous sample and the complexity of resolving UV-Vis spectra effectively, a combinatory method based on a self-made radical electric focusing solid phase extraction (REFSPE) device, UV-Vis detection and partial least squares (PLS) calculation is proposed here. In this study, REFSPE was used to enhance the extraction process of analytes between the aqueous phase and the membrane phase to enrich the trace of mixed organic acid efficiently. Then, the analytes, which were eluted from the adsorption film by ethanol with the assistance of an ultrasonic cleaning machine, were detected with UV-Vis spectrophotometry. After that, the PLS method was introduced to solve the problem of overlapping peaks in UV-Vis spectra of mixed substances and to quantify each compound. The linearly dependent coefficients between the predicted value of the model and the actual concentration of the sample were all higher than 0.99. The limit values of detection for benzoic acid, phthalic acid and p-toluene sulfonic acid were found at 9.9 μg/L, 12.2 μg/L and 13.8 μg/L with the relative recovery values between 84.8% and 117.9%. The RSD (n = 20) values of each component are 1.17%, 1.11% and 0.86%, respectively. Therefore, the proposed combined method can determine traces of complex materials in an aqueous sample efficiently and has wonderful potential applications.
Źródło:
Metrology and Measurement Systems; 2018, 25, 2; 317-329
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
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