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Wyszukujesz frazę "Lipiec, E." wg kryterium: Wszystkie pola

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    Wyświetlanie 1-14 z 14
    Tytuł:
    FTIR Microspectroscopy in Studies of DNA Damage Induced by Proton Microbeam in Single PC-3 Cells
    Autorzy:
    Lipiec, E.
    Kowalska, J.
    Lekki, J.
    Wiecheć, A.
    Kwiatek, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/1490193.pdf
    Data publikacji:
    2012-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    87.14.gk
    87.64.km
    78.30.Jw
    87.53.Bn
    Opis:
    In recent years, the Fourier transformed infrared spectroscopy is often applied in studies of biological materials on cellular level. Undoubted advantage of this method is high sensitivity. In presented research the FTIR microspectroscopy was used to analyse the DNA damage in single PC-3 cells (prostate cancer cell line derived from bone metastases) irradiated by counted number of protons. Focused proton microbeam 2 MeV from the Van de Graaff accelerator at the Institute of Nuclear Physics, Polish Academy of Sciences, was used as an irradiation source. Four groups of single cells were irradiated with 1000, 2000, 4000, and 8000 protons per cell, respectively. Following irradiation cells were fixed in 70% ethanol and then analyzed by IR microspectroscopy. Bond analysis of IR spectra served as a base for result analysis. This research has focused on the detection of changes in DNA backbone spectral range (950-1240 $cm^{-1}$), which could be related to damages such as single and double strand breaks, DNA-DNA, and DNA-protein cross links. Switches and differences in intensity of DNA backbone bands (980-1149 $cm^{-1}$, 1151-1350 $cm^{-1}$ - symmetric and asymmetric $PO^{2-}$ stretching vibrations, as well as in 1110 $cm^{-1}$ - symmetric stretching of P-O-C band) were observed. Experimental spectra of irradiated and control cells were compared with simulated spectra generated by HyperChem software. The multivariate statistical methods of principal component analysis and hierarchical cluster analysis (Ward's method) were also performed and are discussed.
    Źródło:
    Acta Physica Polonica A; 2012, 121, 2; 506-509
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Infrared Spectroscopy in Molecular Study of the Piezoelectric Effect in Pigs Shin Bone
    Autorzy:
    Lipiec, E.
    Kowalska, J.
    Wiecheć, A.
    Zieliński, P.
    Kwiatek, W.
    Iwaniec, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1490248.pdf
    Data publikacji:
    2012-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    78.30.Jw
    07.57.Ty
    77.65.-j
    87.50.ct
    Opis:
    Despite growing number of literature demonstrating the piezoelectric effect in bones at macro level there's still a lack of papers describing this effect at molecular level. In order to examine this effect more specifically, a study has been conducted to show the possibility of using infrared spectroscopy on samples contained in the electric field. This is the first known research on applying infrared spectroscopy to study the influence of electric field and compressive stress on bones' structure represented by the molecular IR spectrum. The samples used in this experiment as a model were prepared in pellet form, made from powdered pig's shin bone mixed with KBr (mass ratio of about 1:100). The spectra were obtained in transmission mode. Three different types of experiments were performed at each sample, which were subjected to: (a) fixed electric field (E < 40 kV/m), (b) variable electric field (0-40 kV/m), (c) compressive stress (P < 100 MPa). The obtained data have shown that the electric field and compressive stress on sample modified infrared spectra of the bone. Qualitative as well as quantitative changes in the spectral range between 900 $cm^{-1}$ and 1200 $cm^{-1}$ and the band at 562 $cm^{-1}$ ($PO_4^{3-}$ asymmetric and symmetric stretching, respectively) were observed. The relative area obtained by decomposition of the $PO_4^{3-}$ band reaches an extreme value in the external compressive stress (10 MPa), that occur under physiological conditions. Based on presented data, it was proved that the applied factors (electric field, compressive stress) can change the oscillation energy and the number of molecule's degrees of freedom.
    Źródło:
    Acta Physica Polonica A; 2012, 121, 2; 539-542
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of Methods in Studies of Cell Death Mechanisms
    Autorzy:
    Borkowska, A.
    Nowakowski, M.
    Miszczyk, J.
    Lipiec, E.
    Wiltowska-Zuber, J.
    Rawojć, K.
    Kwiatek, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/1030322.pdf
    Data publikacji:
    2018-02
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    growth and division
    cell processes
    fluorescence
    optimization
    Opis:
    While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non- cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.
    Źródło:
    Acta Physica Polonica A; 2018, 133, 2; 263-266
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of soil compaction on root growth and crop yield in Central and Eastern Europe
    Autorzy:
    Lipiec, J.
    Medvedev, V.V.
    Birkas, M.
    Dumitru, E.
    Lyndina, T.E.
    Rousseva, S.
    Fulajtar, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/26081.pdf
    Data publikacji:
    2003
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    root function
    root-shoot relation
    crop yield
    root growth
    soil compaction
    Europe
    Źródło:
    International Agrophysics; 2003, 17, 2
    0236-8722
    Pojawia się w:
    International Agrophysics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gruźlica u ludzi i zwierząt - aktualne dane epidemiologiczne
    Tuberculosis in humans and in animals - current epidemiological data
    Autorzy:
    Krajewska, M.
    Kozinska, M.
    Kubajka, M.
    Weiner, M.
    Augustynowicz-Kopec, E.
    Belkot, Z.
    Lipiec, M.
    Szulowski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/859658.pdf
    Data publikacji:
    2015
    Wydawca:
    Krajowa Izba Lekarsko-Weterynaryjna
    Źródło:
    Życie Weterynaryjne; 2015, 90, 10
    0137-6810
    Pojawia się w:
    Życie Weterynaryjne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Human as a potential vector of bovine tuberculosis in cattle
    Autorzy:
    Krajewska-Wedzina, M.
    Weiner, M.
    Anusz, K.
    Augustynowicz-Kopeć, E.
    Lipiec, M.
    Szulowski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085020.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    bovine tuberculosis
    Mycobacterium bovis
    epidemiology
    zoonosis
    mycobacteriology
    public health
    transmission
    tuberculosis
    Opis:
    Introduction and objective. Bovine tuberculosis (bTB, bovine TB) is caused by mycobacteria which are grouped within the MTBC. TB in animals is a highly infectious and progressive disease which can be transmitted to humans. Since 2009, Poland has gained official bTB-free status. Despite the official fact of bTB-free status, a dozen bTB outbreaks are still noted each year. Since 2000 in Poland, every year 1/5 of the national herd is subject to intradermal skin TB testing to control the bTB outbreaks in the cattle population. Application, with 5-year intervals between each government-funded skin test, undoubtedly resulted in financial savings. However it also seems to have caused several adverse and worrying events, e.g. an increase in the number of reactors detected and removed from a single tested herd. The objective of this study was the examination of 898 cattle imputed with bTB infection in Poland between 2008–2012. Materials and method. The study concerned a potential epidemic outbreak with suspected bTB transmission. 20 cows came from 3 herds in the same county located in the same province in southern Poland. Results. 134 MTBC strains were identified. In MIRU-VNTR, all isolates showed the same genetic pattern 322532243421232. Based on molecular investigation, the characteristics of M. bovis strains isolated from cattle from 3 different herds confirmed the common source of this zoonotic disease. Conclusions. Although not bacteriologically proven, everything points to the fact that humans were the vector of bovine tuberculosis transmission between herds. This finding confirms transmission between 3 cattle herds in the Małopolskie Province in southern Poland (Podhale). The outbreak of tuberculosis in animals finally compromised public health.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 3; 396-399
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Druga Międzynarodowa Multi Conference on Engineering and Technological Innovation (10-13 lipiec 2009, Orlando, Florida, USA)
    II International Multi Conference on Engineering and Technological Innovation (10-13 July 2009, Orlando, Florida, USA)
    Autorzy:
    Skawinska, E.
    Zalewski, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/879816.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Ekonomiki Rolnictwa i Gospodarki Żywnościowej - Państwowy Instytut Badawczy
    Źródło:
    Zagadnienia Ekonomiki Rolnej; 2010, 1
    0044-1600
    2392-3458
    Pojawia się w:
    Zagadnienia Ekonomiki Rolnej
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new concept for evaluating muscle function in the lower extremities in cases of low back pain syndrome in anamnesis
    Autorzy:
    Lisinski, P.
    Huber, J.
    Ciesielska, J.
    Lipiec, J.
    Kulczyk, A.
    Bandosz, A.
    Zukiewicz-Sobczak, W.
    Mojs, E.
    Samborski, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/51695.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2014, 21, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zmienność występowania opadów deszczu i śniegu w Hornsundzie w okresie lipiec 1978 - grudzień 2002
    Variability in the occurrence of rain and snow at Hornsund over the period July 1978 - December 2002
    Autorzy:
    Łupikasza, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/260943.pdf
    Data publikacji:
    2003
    Wydawca:
    Stowarzyszenie Klimatologów Polskich
    Tematy:
    Spitsbergen
    opady atmosferyczne
    opady deszczu
    opady śniegu
    rain
    snow
    Opis:
    In the polar regions, the sums of precipitation of the accumulation period is of great importance, because they are an component one of the most valid processes connected with glacier mass balance - accumulation. From glaciological point of view, to determine which part of annual sums of precipitation appears in the form liquid and mixed and solid precipitation is the crucial problem. In this connection, the main aim of this paper is analyse variability of the precipitation with respect of their form in the annual course as well as their long-term variability in Hornsund in the period of 1978-2002. The data used in this article are mainly derived from meteorological yearbook Hornsund (2000, 2001) that was published by Institute of Meteorology and Water Management - maritime department in Gdynia and from yearbook Meteorological Conditions Hornsund, Spitsbergen (2001) that was published by Polish Academy of Science in Warsaw. Collected numerical data are not complete. There is a lack of data concerned precipitation sums and meteorological phenomenon for the period from 01sth to 26sth of July 1981. Moreover the data describing precipitation sums does not exist for the days: 04, 07, 09, 19 July 1979. In this article three main form of precipitation (liquid, mixed and solid) were considered. The form of precipitation was determined on the base of meteorological phenomenon that was recorded as current and last weather. The average, maximum and minimum daily air temperatures were additional criterions. In this connection that the notation of meteorological data prevented to consider each event of appearance of precipitation, the days with individual form of precipitation were subjected to analysis. The day with mixed precipitation means that during all 24 hour period (from 6 given to 6 next day) appeared one time snow and second time rain or snow and rain in the same time. The annual course of individual form of precipitation and their probability of appearance in each month, long term variability of sums of individual form of precipitation and the number of days with this form of precipitation in the accumulation and annual periods were discussed. The linear trends and variability coefficients were calculated for long term characteristics of precipitation. It was stated that in Hornsund long-term average sum of annual precipitation amounted at about 424 mm The greatest part of this appear in the liquid (37%) and the lowest in a mixed form. On average in Hornsund there is noted at about 239 days with precipitation in year from what 63% falls on days with snow and 13% on days with snow and rain. In the accumulation period the solid precipitation were characterized by greatest sums as well as greatest number of days with this one. Long-term course of individual form of precipitation shows similar tendencies. In the annual period as well as in the accumulation this one liquid precipitation are characterized by the highest variability coefficient and range of change. Their lowest sums occur in the middle of 80ies and from mid-80ies they show increasing tendency. Mixed precipitation shows the smallest range of change, their highest sums occur in the middle of 80ies and in the case of annual period also in the second half of 90ies. From the second half of 90ies mixed precipitation show decreasing tendency. Statistically significant increasing tendencies of liquid and mixed precipitation during all considered period accompany decreasing tendency of solid precipitation. The highest sums of solid precipitation occur in the first half of 80ies and the lowest one in the second half of the same decade. From the beginning of 90ies there is a decreasing tendency in the course of solid precipitation. During all considered period the solid precipitation dominate as regards number of days with precipitation. The number of days with solid precipitation until the end of 80ies was greater and in the 90ies was lower than long-term average.
    Źródło:
    Problemy Klimatologii Polarnej; 2003, 13; 93-105
    1234-0715
    Pojawia się w:
    Problemy Klimatologii Polarnej
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microstructure of Welded Joints of X5CrNiCuNb16-4 (17-4 PH) Martensitic Stainlees Steel After Heat Treatment
    Mikrostruktura złącz spawanych stali nierdzewnej martenzytycznej X5CrNiCuNb16-4 (17-4PH) po obróbce cieplnej
    Autorzy:
    Ziewiec, A.
    Zielińska-Lipiec, A.
    Tasak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/355711.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    weld metal
    heat treatment
    martensitic PH stainless steels
    17-4PH steel
    spoiwa
    obróbka cieplna
    stale nierdzewne martenzytyczne PH
    stal 17-4 PH
    Opis:
    The paper presents rezults of microstructure (LM, TEM) investigation and hardness measurments of welded joints in martensitic precipitation hardened stainless steel containing copper, subjected to heat treatment. For the aging temperature up to 540°C even for the very long times, the microstructure of the welded joints is similar to this one at lower temerature aging. After aging at 620°C a distinct change of the microstructure was observed. Non-equilibrium solidification conditions of the weld metal, segregation and the diffusion of copper and the elements stablilizing the austenite cause the occurrence of the reverse transformation of the martensite into austenite as fast as just 1 hour at 620°C. TEM investigations revealed the differences in dispersion of hardening copper precipitates after aging at temperature 620°C for 1 and 4 hours.
    W artykule przedstawiono wyniki badań mikrostruktury (LM, TEM) oraz twardości złącz spawanych stali nierdzewnej martenzytycznej utwardzanej wydzieleniowo miedzią po obróbce cieplnej. Aż do temperatury starzenia 540° C mikrostruktura spoin nie wykazuje istotnych różnic niezależnie od czasu wytrzymania w porównaniu do tej obserwowanej w niższych temperaturach. Po starzeniu w temperaturze 620°C zaobserwowano wyraźne różnice w mikrostrukturze spoin. Nierównowagowe warunki krzepnięcia metalu spoiny, występowanie segregacji oraz procesy dyfuzji miedzi i pierwiastków stabilizujących austenit powodują wystąpienie przemiany odwrotnej martenzytu w austenit już po 1 godzinie starzenia w 620°C. Badania TEM wykazały, różnice w dyspersji cząstek umacniających w temperaturze starzenia 620°C przez 1 i 4 godziny.
    Źródło:
    Archives of Metallurgy and Materials; 2014, 59, 3; 965-970
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-14 z 14

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