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Wyszukujesz frazę "Klimek, Barbara" wg kryterium: Wszystkie pola

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Wyświetlanie 1-10 z 16
Tytuł:
Progesterone biosynthesis supported by fatty acid oxidation in the mitochondrial fraction of human term placenta
Autorzy:
Tiałowska, Barbara
Klimek, Jerzy
Żelewski, Leon
Powiązania:
https://bibliotekanauki.pl/articles/1046080.pdf
Data publikacji:
1983
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1983, 30, 1; 11-21
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotype-specific human papillomavirus detection in cervical smears
Autorzy:
Szostek, Slawa
Klimek, Malgorzata
Zawilinska, Barbara
Kosz-Vnenchak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1040671.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
human papillomavirus
cervical carcinoma
HPV genotyping
squamous intraepithelial lesions
Opis:
Human papillomavirus (HPV) is widely accepted as a causative agent of cervical cancer. The distribution and prevalence of HPV types depend on geographic region and demographic factors. The aim of this study was to investigate the relationship between the presence of various HPV types and the outcome of cytological examination. Cervical smears were obtained from 125 women from southern Poland: low grade squamous intraepithelial lesions (LSIL) - 44, high grade squamous intraepithelial lesions (HSIL) - 12, cervical carcinoma - 27 and 42 women without abnormality in cytology as a control group. DNA was extracted from the smears and broad-spectrum HPV DNA amplification and genotyping was performed with the SPF 10 primer set and reverse hybridisation line probe assay (INNO-LiPA HPV Genotyping, Innogenetics). HPV DNA was detected in approximately 72% cases, more frequently in women with squamous intraepithelial lesions and cervical carcinoma than in the control group (P < 0.0005). The most frequent type found was HPV 16 (37%), followed by HPV 51 (28%) and HPV 52 (17%). A single HPV type was detected in 51% positive cases, more frequently in cervical cancer specimens. Multiple HPV infection was dominant in women with LSIL and normal cytology. Prevalence of HPV 16 increased with the severity of cervical smear abnormality. For women HPV 16 positive, the relative risk (odds ratio) of the occurrence of HSIL and cervical cancer versus LSIL was 14.4 (95% CI, 3.0-69.2; P=0.001) and 49.4 (95% CI, 6.5-372.8; P < 0.001), respectively. Genotyping of HPV will allow better classification of women with cervical abnormalities into different risk groups and could be useful in therapy.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 687-692
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhibition by fatty acids of the cholesterol side-chain cleavage in the course of progesterone biosynthesis in the mitochondrial fraction of human term placenta
Autorzy:
Tiałowska, Barbara
Klimek, Jerzy
Żelewski, Leon
Powiązania:
https://bibliotekanauki.pl/articles/1046164.pdf
Data publikacji:
1980
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1980, 27, 3-4; 257-264
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers
Autorzy:
Szostek, Slawa
Klimek, Malgorzata
Zawilinska, Barbara
Rys, Janusz
Kope, Jolanta
Daszkiewic, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1041225.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
hybrid capture
PCR
HPV detection
papillomavirus
Opis:
The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n = 42) and abnormal (n = 83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (χ2, P < 0.0005). The correlation between assays with the use of SPF10 and MY09/MY11 was 86% and between hybrid capture and the pU-1M/pU2R technique - 78%. In 49% of samples HPV DNA was detected by the four methods, whereas in 12% only by the SPF10 primers. The most sensitive technique was found to be PCR with the use of SPF10 primers, while the most specific - the MY09/11 PCR method. It seems that home-PCR with MY09/MY11 primers could be applied in screening tests.
Źródło:
Acta Biochimica Polonica; 2006, 53, 3; 603-607
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Use of Chosen Biological Methods for Forest Soil Revitalization in Scots Pine Cultivation
Autorzy:
Klimek, Andrzej
Rolbiecki, Stanisław
Rolbiecki, Roman
Gackowski, Grzegorz
Stachowski, Piotr
Jagosz, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/1811739.pdf
Data publikacji:
2020
Wydawca:
Politechnika Koszalińska. Wydawnictwo Uczelniane
Tematy:
forest litter
mite
moss mite
mulching
mycorrhizal preparation
Pinus sylvestris
Opis:
The purpose of this investigation was to indicate the most effective method of revitalizing degraded forest area. The different modifications of wood chips were used as a substitute for humus layer. The mites (Acari), and moss mites (Oribatida) as the bio-indicators of soil succession changes were used. The study began 2 years after planting of Scots pine seedlings on the land devastated by military activity, at the former military training ground (GPS: 53.156943N, 17.986440E). The soil on this area was in the type of rusty soils and subtype of the rusty algae soils. Scots pine seedlings were planted in spring 2011, in a distance 1.5×0.8 m. In one-factor experiment four soil mulching treatments were tested: 1. uncovered soil – control (C), 2. soil mulched with wood chips (W), 3. W + mycorrhizal preparation (WM), 4. W + forest litter (WL). Three replicates of microplots arrangement was applied. Each microplot was 5 m long with 3 rows of Scots pine. Each replication covers 10 rows. Mulching with wood chips was carried out on April 12, 2012. On October 25, 2012, the wood chips on the WM microplots were inoculated with the mycorrhizal biopreparation, and on the WL microplots, a 10% addition of fresh forest litter from the ripe fresh coniferous forest was applied. After the end of the growing season of 2012, 2013 and 2014, the measurement of the plants was carried out (the height, the root neck diameter, the length of one-year increments in the last whorl, the number of one-year increments of the last whorl and the lengths of one-year increments of the last whorl). The samples for acarological tests were collected four times. In total, 40 substrate samples with a volume of 50 cm3 each were collected from each treatment. Mites extraction was carried out for 7 days in Tullgren apparatus. Mites were identified to the order, and moss mites to the species or genus, including juvenile stages. Calculated: the average density of mites, the dominance index, the species richness, the diversity of moss mites, the average number of species, and the Shannon general species diversity index. The use of soil mulching with the Scots pine wood chips did not significantly affect the growth and developmental characteristics of the Scots pine plants. After mulching with wood chips, the total number of mites increased many times, and moss mites began to dominate among micro-arthropods. Mulching treatments increased the number and the species diversity of moss mites in the substrate. The number of moss mites increased the most in wood chips without additives. The highest species diversity was observed in the wood chips with the addition of forest litter. Among moss mites Tectocepheus velatus visibly dominated in all study treatments. Oppiella nova and Scutovertex sculptus also constituted numerous mites populations. The study shows that the wood chips are very useful for use in the regeneration of the devastated and degraded forest soils.
Źródło:
Rocznik Ochrona Środowiska; 2020, Tom 22, cz. 2; 1097-1115
1506-218X
Pojawia się w:
Rocznik Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Management of biomass of selected grape leaves varieties in the process of methane fermentation
Autorzy:
Klimek, Kamila E.
Wrzesińska-Jedrusiak, Edyta
Kapłan, Magdalena
Łaska-Zieja, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/2174282.pdf
Data publikacji:
2022
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
biogas plant
biomass utilisation
methane fermentation
methane biogasification
plant biomass
renewable energy
Opis:
Biogas plants are one of the most stable sources of renewable energy. Currently, there is a noticeable increase in the amount of post-production residues from agricultural production and agri-food processing (fruit and vegetable processing, fermentation, beet pulp, or lignocellulosic waste), which, can be used for biogas production after appropriate pretreatment. The aim of this study was to examine the possibility of using the biomass produced during the cultivation of grapes on a selected farm as a substrate for a biogas plant, taking into account the production process. The research was carried out in 2018-2020 in a vineyard located in the Sandomierz Upland in the south-eastern part of Poland. Own rooted vines were grown as a single continuous string with a trunk height of 40 cm and a length of one fixed arm approx. 0.9 m, on which six pivots were left every year after applying a short cut, from which 12-16 fruit shoots were derived, the so-called grapevines. Leaves were collected at random from three locations on the fruiting shoot, a total of 30 leaves in each replicate. Each sample consisted of 1/3 of the leaves collected at the bottom, 1/3 in the middle, and 1/3 at the top of the canopy. Leaf area was estimated with a model 3100 area meter on a sample of 30 leaves from each replicate. Both the quantity and quality of the obtained material as a substrate for methane fermentation were evaluated. Biogas yield tests in optimal conditions for mesophilic bacteria were conducted on three substrate samples referred to as ‘Regent’, ‘Seyval Blanc’, and ‘Solaris’. The yields of the tested material ranged from 51.0 to 59.0 Nm3 biogas per Mg of biomass.
Źródło:
Journal of Water and Land Development; 2022, 55; 17--27
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Energy expenditure of athletes endurance and strength in the light of the Polish energy intake standards
Autorzy:
Frączek, Barbara
Grzelak, Andrzej
Klimek, Andrzej T.
Powiązania:
https://bibliotekanauki.pl/articles/2161987.pdf
Data publikacji:
2019-02-27
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
athletes
heart rate monitoring
daily energy expenditure
training energy expenditure
endurance sports
power sports
Opis:
Objectives The purpose of the study was to estimate training energy expenditure (TEE) and daily energy expenditure (DEE) in the Polish elite athletes engaged in endurance sports and power sports, and to compare their levels with the Polish energy intake standards. Material and Methods The study sample consisted of 30 athletes (15 women and 15 men) whose energy expenditure was estimated based on heart rate monitoring. Results The mean values of DEE obtained for women engaged in endurance sports (WE – women endurance) and power sports (WP – women power) were 3201±533 kcal and 3217±554 kcal, respectively. In the group of male athletes, the respective values were 4518±717 kcal for endurance athletes (ME – men endurance) and 4459±837 kcal for power athletes (MP – men power). The mean values of TEE for female athletes were 819±240 kcal (WE) and 1047±248 kcal (WP). Those obtained for male athletes were significantly higher: 1440±675 kcal (ME) and 1337±331 kcal (MP). Conclusions Unlike the demands of particular sport disciplines, an athlete’s sex proved to be a factor causing significant differences between the TEE and DEE of athletes representing different sport disciplines. Individual athletes were found to differ significantly in their demand for energy, which in some cases was considerably different from what energy intake standards propose. Int J Occup Med Environ Health. 2019;32(1):1–13
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2019, 32, 1; 1-13
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of single whole body cryostimulation treatment on the dynamics and the level of maximal anaerobic power
Autorzy:
Klimek, Andrzej T.
Lubkowska, Anna
Szyguła, Zbigniew
Frączek, Barbara
Chudecka, Monika
Powiązania:
https://bibliotekanauki.pl/articles/2185364.pdf
Data publikacji:
2011-06-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
cryostimulation
cryogenic temperature
hypothermia
maximal anaerobic power
anaerobic capacity
Opis:
Objectives: The objective of this work was to determine the dynamics of maximal anaerobic power (MAP) of the lower limbs, following a single whole body cryostimulation treatment (WBC), in relation to the temperature of thigh muscles. Materials and Methods: The subjects included 15 men and 15 women with an average age (±SD) of 21.6±1.2 years. To evaluate the level of anaerobic power, the Wingate test was applied. The subjects were submitted to 6 WBC treatments at -130°C once a day. After each session they performed a single Wingate test in the 15, 30, 45, 60, 75 and 90th min after leaving the cryogenic chamber. The order of the test was randomized. All Wingate tests were preceded by an evaluation of thigh surface temperature with the use of a thermovisual camera. Results: The average thigh surface temperature $(\text{T}_\text{av})$ in both men and women dropped significantly after the whole body cryostimulation treatment, and next increased gradually. In women $\text{T}_\text{av}$ remained decreased for 75 min, whereas in men it did not return to the basal level until 90th min. A statistically insignificant decrease in MAP was observed in women after WBC. On the contrary, a non-significant increase in MAP was observed in men. The course of changes in MAP following the treatment was similar in both sexes to the changes in thigh surface temperature, with the exception of the period between 15th and 30th min. The shorter time to obtain MAP was observed in women till 90th min and in men till 45 min after WBC compared to the initial level. Conclusions: A single whole body cryostimulation may have a minor influence on short-term physical performance of supramaximal intensity, but it leads to improvement of velocity during the start as evidenced by shorter time required to obtain MAP.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2011, 24, 2; 184-191
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physical state of human papillomavirus type 16 in cervical intraepithelial lesions and cancers determined by two different quantitative real-time PCR methods
Autorzy:
Szostek, Slawa
Biesaga, Beata
Zawilinska, Barbara
Klimek, Malgorzata
Kosz-Vnenchak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1038944.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
real-time PCR
human papillomavirus
squamous intraepithelial lesions
cervical carcinoma
Opis:
The aim of this study was to analyse the correlation between a new multiplex qPCR assay and a reference qPCR assay for assessment of the human papillomavirus (HPV16) load and the viral genome status. The study was performed on 100 HPV16 positive samples containing premalignant lesions and carcinomas. HPV16 E2 and E6 gene loads were assessed by two PCR methods. The load of E2 and E6 was normalized to the cell number by qPCR targeting the RNase P open reading frame. The physical state of the viral genome was determined as a ratio of E2/E6 copies number per cell. Among 100 samples analysed, there were no statistically significant differences in the E2 and E6 viral load evaluated by multiplex qPCR and qPCR, the correlation coefficients were 0.98 and 0.97, respectively. There were 19% of samples with the integrated, 73% with mixed and 8% with episomal state of viral genome detected by multiplex qPCR and 17%, 79%, 4%, respectively, found by qPCR. Prevalence of integrated and episomal forms estimated by multiplex qPCR was higher than the one obtained by qPCR (Chi2, p < 0.0001), but in samples with premalignant and malignant diagnoses no significant differences were demonstrated regardless of the methods used. Sensitivity and specificity of multiplex qPCR were 93.7% and 100% as compared with qPCR, the positive predictive value was 100%. In summary, the multiplex qPCR assay in respect of HPV16 load and the frequency of viral genome status was shown to be a sensitive and specific reference method. Simultaneous estimation of E2 and E6 genes in one reaction tube reduces the cost of testing.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 923-928
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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