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    Wyświetlanie 1-6 z 6
    Tytuł:
    Zapłodnienie in vitro u roślin kwiatowych
    In vitro fertilization (IVF) in flowering plants
    Autorzy:
    Mól, Rafał
    Powiązania:
    https://bibliotekanauki.pl/articles/1201657.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Opis:
    This review deals with the recent progress in studies on double fertilization in angiosperms. It briefly presents the IVF-methods and their impact on solving some questions asked by plant embryologists. In the past decade, electrofusion, Ca2+-mediated or PEG-mediated fusion of plant gametes, as well as molecular and immunocytological studies on isolated sperm cells, egg cells, central cells and zygotes, yielded very important new results concerning fertilization in higher plants usingmaize as amodel plant. The first of cellular events that occurs after gamete fusion is an increase of cytosolic Ca2+ concentration, known already in animals but found only recently in flowering plants. Egg cytoplasm activation appears to follow also the fusion of plant gametes but still needs to be demonstrated that the rise of cytosolic calcium is necessary and/or sufficient for the egg activation. Another similarity between the course of fertilization in plants and animals is the block to polyspermy. This was shown to occur in maize by Ca2+-mediated gamete fusion in vitro and possibly depends on resynthesis of the egg cell wall. If an early block at themembrane level also exists, as in animals, needs to be elucidated. Maize sperm and egg electrofusion gave precise data on the karyogamy kinetics in plants, and studies on DNA content in isolated eggs and zygotes proved that the DNA synthesis occurs after gamete fusion, i.e. during zygote maturation. Unlike in animals, molecules responsible for sperm-egg interactions have not yet been identified. However, several sperm-, egg- and zygote-specific DNA sequences were found in maize and the very recent data suggest that the glycoproteins of tobacco gamete membranes might be involved in fertilization. Despite of this tremendous work done on isolated gametes, the question remains open if double fertilization is determined or random.
    Źródło:
    Kosmos; 2003, 52, 4; 459-467
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA)
    Kwitnienie i mikropropagacja in vitro lisianthusa (Eustoma grandiflorum) w reakcji na regulatory wzrostu roslin (NAA i BA)
    Autorzy:
    Kaviani, B.
    Zamiraee, F.
    Zanjani, S.B.
    Tarang, A.
    Torkashvand, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542950.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    in vitro culture
    ornamental plant
    flowering
    micropropagation
    Lisianthus
    Eustoma grandiflorum
    plant response
    plant growth regulator
    NAA potassium salt
    axillary bud
    callus
    plant tissue culture
    Opis:
    In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.
    Kwitnienie i mikropropagacja in vitro są użyteczne w programach hodowli roślin oraz produkcji komercyjnej ważnych roślin ozdobnych. Warunki in vitro, łącznie ze składnikami pożywek, rodzajem, stężeniem oraz proporcją regulatorów wzrostu roślin, a także warunkami hodowli, w sposób istotny wpływają na kwitnienie i mikropropagację in vitro. Nie istnieje żadne badanie dotyczące kwitnienia in vitro lisanthiusa (Eustoma grandiflorum). W niniejszym badaniu opracowano kwitnienie i wysoką częstotliwość mikropropagacji in vitro dla E. grandiflorum, który jest rośliną ozdobną. Mikropropagacja jest skutecznym narzędziem rozmnażania roślin ozdobnych na dużą skalę. Celem niniejszego badania była ocena wpływu różnych stężeĔ NAA i BA na mikropropagację i kwitnienie lisianthiusa in vitro. Używana pożywka hodowlana została wzbogacona za pomocą 0; 0,1; 0,2 i 2 mg L-1 NAA i BA. Przy powstawaniu eksplantów największa długość łodygi (2,07 cm na roślinę) była uzyskana na pożywce uzupełnionej o 0,1 mg L-1 BA (bez NAA). Maksymalna liczba łodyg (5,80 na roślinę) została wytworzona na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,2 mg L-1 NAA. Eksplanty pączków na pożywce hodowlanej zawierającej 0,2 mg L-1 NAA (bez BA) oraz 0,1 mg L-1 NAA wraz z 2 mg L-1 BA wytworzyły maksymalną liczbę węzłów (3,20 na roślinę). Największą liczbę korzeni (14,53 na roślinę) oraz największą długość korzenia (3,87 na roślinę) zaobserwowano na 0,2 mg L-1 NAA bez BA jak również 0,2 mg L-1 BA plus 0,2 mg L-1 NAA oraz 0,2 mg L-1 BA bez NAA. Eksplanty tworzyły kwiat na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,1 mg L-1 NAA bez przeniesienia kalusa. Kwiat był tworzony z kalusa na pożywce zawierającej 0,1 mg L-1 BA wraz z 2 mg L-1 NAA. Zregenerowane rośliny wykazały 98% przeżycie w szklarni podczas aklimatyzacji. Zaaklimatyzowane rośliny były morfologicznie podobne to swych roślin macierzystych.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 145-155
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Implications of plant growth regulators on induction of in vitro flowering in Aerva lanata (L.) Juss. ex Schult.
    Autorzy:
    Shekhawat, Mahipal S.
    Manokari, M.
    Ravindran, C. P.
    Powiązania:
    https://bibliotekanauki.pl/articles/1186752.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Aerva lanata
    In vitro flowering
    Spectral flux photon density
    Opis:
    In vitro flowering was successfully induced from the shoots regenerated in cultures of Aerva lanata. In vitro flowering method can be monitored effectively in studying floral transition and flower development from vegetative to reproductive phase. The nodal shoot segments were used as explants in the present study. The explants were surface sterilized using HgCl2 and inoculated on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP) for bud break. Maximum numbers of shoots were induced on MS medium supplemented with 1.0 mg l-1 BAP. The cultures were multiplied by continuous subculturing of shoots with mother explants on fresh MS medium with BAP and Kinetin (Kin). Rooting was achieved on one fourth MS medium supplemented with 2.0 mg l-1 indole-3-butyric acid (IBA). The multiplied shoots were subjected to various concentrations of different plant growth regulators and photoperiod regimes to induce flowering in vitro. The maximum numbers of in vitro flowers were recorded from the shoots on full strength MS medium with 0.5 mg l-1 BAP and 0.1 mg l-1 indole-3-acetic acid (IAA) on 12/12 h/d photoperiod.
    Źródło:
    World Scientific News; 2016, 32; 71-81
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems
    Autorzy:
    Kaviani, B.
    Bahari, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/12678778.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Gentianaceae
    Eustoma
    Lisianthus
    Eustoma grandiflorum
    ornamental plant
    flowering plant
    rooting
    microshoot
    micropropagation
    in vitro proliferation
    ex vitro method
    soild phase
    liquid phase
    double-phase culture
    plant growth regulator
    auxin
    cytokinin
    Opis:
    A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 47-56
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
    Autorzy:
    Kozak, D.
    Parzymies, M.
    Świstowska, A.
    Marcinek, B.
    Ismael, B.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/12663898.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Brazilian jasmine
    Mandevilla sanderi
    ornamental plant
    flowering plant
    pot plant
    commercial plant
    plant growth
    plant development
    plant regeneration
    explant type
    multiplication
    decapitation
    defoliation
    in vitro culture
    tissue culture
    cultivation experiment
    Opis:
    Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
    Autorzy:
    Marcinek, B.
    Parzymies, M.
    Poniewozik, M.
    Kozak, D.
    Durlak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/12307379.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Dahlia
    Dahlia x cultorum
    dahlia
    ornamental plant
    flowering plant
    tuberous plant
    tuberous root
    plant acclimatization
    growth regulator
    cytokinin
    gibberellic acid
    micropropagation
    tissue culture
    ex vitro method
    microcutting
    Opis:
    The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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