- Tytuł:
- Genetic similarity of chosen Syringa species determined by the ISSR-PCR technique
- Autorzy:
-
Rzepka-Plevnes, D
Smolik, M.
Tanska, K. - Powiązania:
- https://bibliotekanauki.pl/articles/41611.pdf
- Data publikacji:
- 2006
- Wydawca:
- Polska Akademia Nauk. Instytut Dendrologii PAN
- Tematy:
-
Przelewice Dendrological Garden
genetic variability
Syringa
lilac
genetic diversity
taxonomy
ISSR technique
polymerase chain reaction - Opis:
- Inter-simple sequence repeat (ISSR) amplification was used to analyze polymorphism of microsatellite sequences in the lilacs genom and to evaluate genetic diversity among seven lilacs species (Syringa × prestoniae McKelvey., S. reflexa K.C. Schneid., S. villosa Vahl, S. × chinensis Willd., S. meyeri K.C.Schneid., S. vulgaris L.and S. reticulata (Blume) var. amurensis (Rupr.)). The plant material was originated from the collection of Dendrological Garden in Przelewice.A total of 30 primers, containing different simple sequence repeat motifs were tested for amplification.Out of the 30 primers only 13 gave interpretable banding patterns in all lilacs species.A total of 182 ISSR fragments were generated with 13 primers of which 109 (60%) were polymorphic and 57 (31.2%) species-specific. ISSR–PCR with genomic DNAs of the showed lilacs yielded DNA fragmets ranging form 2200 to 123 bp in size.Species-specific ISSR fragments were detected for each lilacs accessions.UPGMA cluster analysis was used to construct a dendrogram and to estimate the genetic distances between lilacs species.The ISSR-based phylogeny was generally consistent with Syringa taxonomy based on morphological and phenological evidence.
- Źródło:
-
Dendrobiology; 2006, 56; 61-67
1641-1307 - Pojawia się w:
- Dendrobiology
- Dostawca treści:
- Biblioteka Nauki
Artykuł