Wszystkie pola: Helicobacter pylori antigens - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: The clinical importance of Helicobacter pylori antigens detected in the dental plaque and feces
Autorzy:: Namiot, A.
Leszczyńska, K.
Namiot, D.
Bucki, R.
Kemona, A.
Chilewicz, M.
Namiot, Z.
Powiązania:: https://bibliotekanauki.pl/articles/1917520.pdf
Data publikacji:: 2015
Wydawca:: Uniwersytet Medyczny w Białymstoku
Tematy:: Helicobacter pylori antigens
Immunoassay
Dental Plaque
Feces
Opis:: Purpose:It is expected that H. pylori residing outside the stomach influences the results of the stool test. The aim of the study was to investigate the occurrence of H. pylori antigens in dental plaque and feces of the patients with H. pylori infected and non-infected stomachs.Materials and methods: The study was conducted in 188 dentate patients, 107 with H. pylori infected and 81 non-infected stomachs. Stomach infection with H. pylori was evaluated with CLO test, histology and culture. The stomach was classified as infected if at least two of three tests (CLO test, culture, histology) were positive and as non-infected if all three tests were negative. Dental plaque was taken only from the natural teeth. On the day of the gastroscopic examination or on the next two days a stool sample was collected for H. pylori antigens testing. H. pylori antigens in dental plaque and feces were determined by immunological method.Results:In 60.8% of subjects with an infected stomach, H. pylori antigens were present both in the dental plaque and feces, in 37.4% in feces only, in 0.9% only in the dental plaque, and in 0.9% neither in the dental plaque nor feces. In 46.9% of subjects with a non-infected stomach, H. pylori antigens were found neither in the dental plaque nor feces, in 24.7% both in the dental plaque and feces, in 23.5% only in the dental plaque, and in 4,9% only in feces.Conclusions: There is a weak association between the occurrence of H. pylori antigens in feces and the dental plaque, and also between the occurrence of the antigens in the dental plaque and stomach infection.
Źródło:: Progress in Health Sciences; 2015, 5, 2; 24-29
2083-1617
Pojawia się w:: Progress in Health Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Application of Helicobacter pylori antigen test to evaluate gastric mucosa specimens
Autorzy:: Namiot, A.
Leszczyńska, K.
Namiot, D.B.
Chilewicz, M.
Bucki, R.
Kemona, A
Namiot, Z.
Powiązania:: https://bibliotekanauki.pl/articles/1916576.pdf
Data publikacji:: 2014
Wydawca:: Uniwersytet Medyczny w Białymstoku
Tematy:: Helicobacter pylori antigens
immunoassay
gastric mucosa
Opis:: Purpose: To investigate, whether the test documenting the presence of Helicobacter pylori (H. pylori) antigens in the gastric mucosa may be used as diagnostic test. Materials and methods: Mucosal specimens taken from eighty-three patients during gastroscopic examination were subjected to rapid urease test (CLO test), histology, and H. pylori culture. The same biopsy specimens that had been evaluated in the CLO test or collected into the transport medium for bacterial culture were used to detect H. pylori antigens. An amplified immunoassay for the detection of H. pylori antigens in stool was used for gastric mucosa specimens. The sensitivity and specificity of the H. pylori antigen test were evaluated in relation to the results of each verifying test (CLO test, histology, culture) separately and to all 3 tests analysed together. Results: The sensitivity and specificity of the H. pylori antigen test in relation to the CLO test, histological examination, and H. pylori culture were 85.4% and 90.5%, 76.1% and 83.4%, and 90.7% and 90.0% for specimens taken for the CLO test and 90.0% and 82.0%, 78.0% and 81.0%, and 93.0% and 88.0% for specimens taken for bacterial culture, respectively. The sensitivity and specificity of the antigen test in relation to all 3 verifying tests analysed together were 91.3% and 97.3% for specimens taken for the CLO test, and 91.7% and 97.1% for specimens taken for bacterial culture, respectively. Conclusions: H. pylori antigen test in gastric mucosa specimens may be a sufficiently reliable source of information about stomach infection.
Źródło:: Progress in Health Sciences; 2014, 4, 2; 52-57
2083-1617
Pojawia się w:: Progress in Health Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Immunoregulation of antigen presenting and secretory functions of monocytic cells by Helicobacter pylori antigens in relation to impairment of lymphocyte expansion
Autorzy:: Mnich, Eliza
Gajewski, Adrian
Rudnicka, Karolina
Gonciarz, Weronika
Stawerski, Paweł
Hinc, Krzysztof
Obuchowski, Michał
Chmiela, Magdalena
Powiązania:: https://bibliotekanauki.pl/articles/1038873.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: H. pylori
antigen presenting cells
lymphocyte expansion
Opis:: The role of Helicobacter pylori (H. pylori) antigens in driving a specific immune response against the bacteria causing gastroduodenal disorders is poorly understood. Using a guinea pig model mimicking the natural history of H. pylori infection, we evaluated the effectiveness of immature and mature macrophages in promoting the blastogenesis of splenocytes from H. pylori infected and uninfected animals, in response to H. pylori antigens: glycine acid extract (GE), cytotoxin associated gene A protein (CagA), urease A (UreA) and lipopolysaccharide (LPS). Lymphocyte expansion was assessed in 72 h cell cultures, containing: immature or mature macrophages derived from bone marrow monocytes, unstimulated or stimulated with H. pylori antigens for 2 h. The proliferation was expressed as a ratio of [3H]-thymidine incorporation into DNA of antigen-stimulated to unstimulated cells and the DNA damage was determined by DAPI cell staining. TGF-β and IFN-γ were assessed immunoenzymatically in cell culture supernatants. Lymphocytes of control and H. pylori-infected animals proliferated intensively in response to phytohaemagglutinin (PHA) and in co-cultures with immature or mature macrophages treated with CagA or UreA (significantly) and GE (slightly) exluding the cultures containing H. pylori or E. coli LPS. This lymphocyte growth inhibition was related to DNA damage of monocytic cells in response to H. pylori or E. coli LPS and secretion of regulatory TGF-β, but not proinflammatory IFN-γ. Impaired homeostasis of monocytic cell function related to DNA damage and TGF-β release, in response to H. pylori LPS may lead to the suppression of adaptive immune response against the bacteria and development of chronic infection.
Źródło:: Acta Biochimica Polonica; 2015, 62, 4; 641-650
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Helicobacter pylori antigens, acetylsalicylic acid, LDL and 7-ketocholesterol - their potential role in destabilizing the gastric epithelial cell barrier. An in vitro model of Kato III cells
Autorzy:: Gajewski, Adrian
Mnich, Eliza
Szymański, Karol
Hinc, Krzysztof
Obuchowski, Michał
Moran, Anthony
Chmiela, Magdalena
Powiązania:: https://bibliotekanauki.pl/articles/1038856.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Helicobacter pylori
acetylsalicylic acid
7-ketocholesterol
gastric barrier
Opis:: Colonization of gastric tissue in humans by H. pylori Gram-negative bacteria initiates gastric and duodenal ulcers and even gastric cancers. Infections promote inflammation and damage to gastric epithelium which might be followed by the impairment of its barrier function. The role of H. pylori components in these processes has not been specified. H. pylori cytotoxicity may potentially increase in the milieu of anti-inflammatory drugs including acetylsalicylic acid (ASA). The lipid transport-associated molecule such as low density lipoprotein (LDL), which is a classic risk factor of coronary heart disease (CHD) and 7-ketocholesterol (7-kCh) a product of cholesterol oxidation, which may occur during the oxidative stress in LDL could also be considered as pro-inflammatory. The aim of this study was to evaluate the cytotoxicity of H. pylori antigens, ASA, LDL and 7-kCh towards Kato III gastric epithelial cells, on the basis of the cell ability to reduce tetrazolium salt (MTT) and morphology of cell nuclei assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Kato III cells were stimulated for 24 h, at 37°C and 5% CO2, with H. pylori antigens: cytotoxin associated gene A (CagA) protein, the urease A subunit (UreA), lipopolysaccharide (LPS) and ASA, LDL or 7-kCh. H. pylori LPS, ASA, LDL and 7-kCh, but not H. pylori glycine acid extract (GE), demonstrated cytotoxicity against Kato III cells, which was related to a diminished percentage of MTT reducing cells and to an increased cell population with the signs of DNA damage. The results suggest that damage to gastric epithelial cells can be induced independently by H. pylori antigens, ASA and endogenous lipid transport-associated molecules. During H. pylori infection in vivo, especially in CHD patients, synergistic or antagonistic interactions between these factors might possibly influence the disease course. Further study is necessary to explain these potential effects.
Źródło:: Acta Biochimica Polonica; 2016, 63, 1; 145-152
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: The influence of Helicobacter pylori eradication therapy on the presence of H. pylori antigens in dental plaque and saliva
Autorzy:: Namiot, D.B.
Leszczyńska, K.
Namiot, A.
Kemona, A.
Bucki, R.
Chilewicz, M.
Namiot, Z.
Powiązania:: https://bibliotekanauki.pl/articles/1917816.pdf
Data publikacji:: 2016
Wydawca:: Uniwersytet Medyczny w Białymstoku
Tematy:: Helicobacter pylori treatment
immunoassay
gastric mucosa
oral cavity
Opis:: Purpose: The aim of this study was to evaluate the presence of H. pylori antigens in the oral cavity (dental plaque and saliva) of patients undergoing systemic eradication therapy. Materials and methods: The study was conducted in 49 subjects with H. pylori stomach infection. H. pylori antigens in dental plaque and saliva were evaluated with immunological method. Results: In subjects with initial H. pylori oral infection, the presence of H. pylori antigens in the oral cavity 6 weeks after successful or unsuccessful H. pylori eradication therapy in the stomach was 47.0% and 50.0%, respectively. In subjects without initial oral infection with H. pylori, the presence of H. pylori antigens in the oral cavity 6 weeks after successful and unsuccessful eradication therapy in the stomach was 30.0% and 20.0%, respectively. Conclusions: The immunological method detecting H. pylori antigens in the dental plaque and saliva cannot be recommended to evaluate the efficacy of H. pylori eradication in the oral cavity.
Źródło:: Progress in Health Sciences; 2016, 6(1); 19-24
2083-1617
Pojawia się w:: Progress in Health Sciences
Dostawca treści:: Biblioteka Nauki