Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "Escherichia coli" wg kryterium: Wszystkie pola

  Lista filtrów
Wyrównaj
Wyświetlanie 1-15 z 209
Tytuł:
Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions
Autorzy:
Adamus-Białek, Wioletta
Kubiak, Anna
Czerwonka, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038911.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biofilm
uropathogenic Escherichia coli
urinary tract infections
Opis:
The ability to form different types of biofilm enables bacteria to survive in a harsh or toxic environment. Different structures of biofilms are related to different surfaces and environment of bacterial growth. The aim of this study was analysis of the biofilm formation of 115 clinical uropathogenic Escherichia coli strains under different growth conditions: surface for biofilm formation, medium composition and time of incubation. The biofilm formation after 24 h, 48 h, 72 h and 96 h was determined spectrophotometrically (A531) after crystal violet staining and it was correlated with bacterial growth (A600). The live and dead cells in biofilm structures was also observed on the glass surface by an epi-fluorescence microscope. Additionally, the presence of rpoS, sdiA and rscA genes was analyzed. The statistical significance was estimated by paired T-test. The observed biofilms were different for each particular strain. The biofilm formation was the highest in the rich medium (LB) after 24 h and its level hasn't changed in time. When biofilm level was compared to bacterial growth (relative biofilm) - it was higher in a minimal medium in comparison to enriched medium. These results suggest that most of the bacterial cells prefer to live in a biofilm community under the difficult environmental conditions. Moreover, biofilm formation on polyurethane surface did not correlate with biofilm formation on glass. It suggests that mechanisms of biofilm formation can be correlated with other bacterial properties. This phenomenon may explain different types of biofilm formation among one species and even one pathotype - uropathogenic Escherichia coli.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 765-771
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of multidrug resistant and extended spectrum β-lactamase (ESBL) - producing Escherichia coli in wastewater of two healthcare facilities in Ibadan, Nigeria
Autorzy:
Adekanmbi, Abimbola O.
Adeyemi, Adedoyin O.
Olajide, Oreoluwa M.
Powiązania:
https://bibliotekanauki.pl/articles/1076664.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Escherichia coli
hospital-waste
wastewater
β-lactamase
Opis:
Most industries in developing countries of the world, especially hospitals and other clinical settings, lack wastewater treatment facilities, and as such, untreated wastewater from their operations are discharged into water bodies without any form of treatment. This study aimed at the antibiotic sensitivity profile and ESBL production in E. coli isolated from untreated hospital wastewater before discharge into the environment. Untreated wastewater from two hospitals, a State Government-owned hospital (SGH) and a privately-owned hospital (POH) with no wastewater treatment facilities were sampled for a period of four months. Isolation of E. coli was carried out using the pour plate technique on Eosin Methylene Blue agar, while identification was carried out using conventional methods. Determination of ESBL production was done by means of the Double Disc Synergy Technique and antibiotic sensitivity testing was carried out by employing the disc diffusion method. A total of fifty-eight (58) E. coli were obtained: SGH at 55 and POH at 3. Herein, in 100% of the total count, resistance was indicated for ampicillin and ertapenem, while 14%, 11%, 16% and 57% of the total count were resistant to ceftazidime, cefpodoxime, cefotaxime and amoxicillin-clavulanate, respectively. In addition, 94.8% showed resistance to tetracycline, 19% to ciprofloxacin, 6.9% to gentamycin, 39.7% to chloramphenicol and 55% and 47% to sulfamethoxazole-trimethoprim and nalidixic acid, respectively. Furthermore, 94.8% of all the isolates were multidrug resistant (MDR), while 29.3% were ESBL positive. Wastewater from the two hospitals under study contained ESBL positive and MDR E. coli, suggesting a need to forestall a potential threat to public health by treating the wastewater generated by both hospitals before discharge into the environment.
Źródło:
World News of Natural Sciences; 2019, 26; 167-175
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Removal of Escherichia coli from Domestic Wastewater Using Electrocoagulation
Autorzy:
Aguilar-Ascon, Edwar
Powiązania:
https://bibliotekanauki.pl/articles/124695.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
domestic wastewater
electrocoagulation
escherichia coli
electrochemical disinfection
Opis:
The objective of this study was to evaluate the efficiency of electrocoagulation in the removal of Escherichia coli from domestic and urban wastewaters and to determine the effects of the main operational parameters on the process. An electrocoagulation reactor with aluminum and iron electrodes was built for this purpose. A factorial design was applied, where amperage, treatment time, and pH were considered as the factors and E. coli percent removal was the response variable. After 20 min of treatment, >97% removal efficiency was achieved. The highest E. coli removal efficiency achieved was 99.9% at a neutral pH of 7, amperage of 3 A, and treatment time of 60 min. However, the removal efficiency of close to 99% was also achieved at natural wastewater pH of 8.5. The statistical analyses showed that the three tested factors significantly affected the E. coli removal percentage (p < 0.05). These results indicate that electrocoagulation has a high disinfection power in a primary reactor in removing water contaminants as well as simultaneously removing pathogenic microorganisms when compared to biological treatment processes. This represents an additional benefit, because it will considerably reduce the use of chlorine during the final disinfection stage.
Źródło:
Journal of Ecological Engineering; 2019, 20, 5; 42-51
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of glycosylated flavonoids extracted from sweet-cherry stems, as antibacterial agents against pathogenic Escherichia coli isolates
Autorzy:
Aires, Alfredo
Dias, Carla
Carvalho, Rosa
Saavedra, Maria
Powiązania:
https://bibliotekanauki.pl/articles/1038644.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
flavonoids
agro-food wastes
enhanced extraction
antimicrobial agents
pathogenic bacteria
Opis:
The aim of this study was to evaluate the bioactivity of flavonoids extracted from sweet-cherry stems which are often used by a traditional system of medicine to treat gastro-intestinal and urinary tract infections but lacking any consistent scientific evidence; moreover the information about the class of phenolics, their content and the potential bioactivity of such material is very scarce. Thus, in this context, we have set a research study in which we evaluated the profile and content of phenolics extracted from sweet-cherry stems through a conventional (70ºC and 20 min) and ultrasound assisted extraction (40 kHz, room temperature and 20 min). The extracts were phytochemically characterized by using an HPLC-DAD-UV/VIS system and assayed by an in vitro minimum inhibitory concentration (MIC) bioassay against Escherichia coli isolates. Simultaneously, the total antioxidant activities were measured using the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS•+) radical cation assay. Our results indicate that sweet-cherry stems have a high content of sakuranetin, ferulic acid, p-coumaric acid, p-coumaroylquinic acid, chlorogenic acid and its isomer neochlorogenic acid. Their average levels were highly affected by the extraction method used (p<0.001). The same trend was observed for total antioxidant activity and MIC values. The extracts produced with ultrasounds presented both, a higher total antioxidant activity and a lower minimum inhibitory concentration. Statistical analyses of our results showed a significant correlation (p<0.01) of total antioxidant activity and minimum inhibitory concentration with phenolics present in the extracts studied. Thus, we can conclude that cherry stems can be further exploited to purify compounds and produce coproducts with enhanced biologically added value for pharmaceutical industry.
Źródło:
Acta Biochimica Polonica; 2017, 64, 2; 265-271
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Silver-clay nanohybrid as a coating for improvement of the antibacterial properties of paper
Autorzy:
Alikhah, Tahereh
Babavand, Arash
Afra, Elyas
Ghasemian, Ali
Saraeian, Ahmadreza
Powiązania:
https://bibliotekanauki.pl/articles/342609.pdf
Data publikacji:
2020
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Drewna
Tematy:
paper coating
silver-clay nanohybrid
antibacterial property
Escherichia coli
Staphylococcus aureus
Opis:
This comparative study was carried out to investigate the effect of nanosilver, silver-clay nanohybrid, and silver-milled clay nanohybrid coatings on the antibacterial characteristics of paper. Nanosilver (25 ppm) was used as a singleand double-layer coating. The results demonstrated that treatment with pure nanosilver was more efficient in the reduction of microbial growth. Also, milling of clay enabled better maintenance of silver nanoparticles, and led to a greater decrease in bacterial growth than in the case of the original silver-clay nanohybrid. Evaluation of the sustainability of antibacterial characteristics confirmed that, although pure nanosilver treatment achieved better performance in the first 15 minutes than nanohybrid samples, the performance of the nanohybrids improved with the passing of time. As expected, the treatments decreased the brightness of paper, while the opacity increased significantly; pure nanosilver treatment led to lower brightness than the others, and the opacity was higher in the case of the silver-clay nanohybrid than with the other treatments.
Źródło:
Drewno. Prace Naukowe. Doniesienia. Komunikaty; 2020, 62, 205; 109-124
1644-3985
Pojawia się w:
Drewno. Prace Naukowe. Doniesienia. Komunikaty
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wound healing potential of Capparis spinosa against cutaneous wounds infected by Escherichia coli in a rat model
Możliwość leczenia ran ciętych zainfekowanych Escherichia coli za pomocą Capparis spinosa na modelu szczurzym
Autorzy:
Asheghian Amiri, I.
Moslemi, H.R.
Tehrani-Sharif, M.
Kafshdouzan, K.
Powiązania:
https://bibliotekanauki.pl/articles/72825.pdf
Data publikacji:
2015
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
wound
cutaneous wound
Escherichia coli
wound infection
treatment
Capparis spinosa
plant extract
ethanol extract
rat
animal model
Opis:
Wound infection has become a major medical problem in recent years. This study was conducted to evaluate the healing activity of Capparis spinosa against surgical wounds infected by Escherichia coli. Twenty male rats were divided into two groups. Excisions were created surgically on the animals’ skin and then infected with E coli. Group 1 was treated with C spinosa while Group 2 was untreated. Wound biopsy specimens were collected on days 5, 10 and 16 and analyzed. Results showed that the hydroxyproline content in treatment group was significantly higher in various post wounding days. Protein content increased gradually in ten days. Results of histopathological studies showed moderate to intense granulation tissue formation in treated group on day 10. The histopathological studies showed, that the new epidermis in treated group was thicker than in control group on day 16 post wounding. The present study has demonstrated that ethanol extract of C spinosa includes properties that accelerate wound healing activities.
Infekcje ran stały się ostatnio dużym problemem medycznym. W prezentowanym artykule przedstawiono badania przeprowadzone w celu określenia działania Capparis spinosa przeciwko infekcjom ran pooperacyjnych przez Escherichia coli. Dwadzieścia szczurów płci męskiej podzielono na dwie grupy. Skórę zwierząt nacięto operacyjnie, a następnie ranę zainfekowano E coli. Grupę 1 leczono C spinosa, grupa 2 nie była leczona. Biopsję ran przeprowadzono 5., 10. i 16. dnia, a następnie poddano analizie. Wyniki wykazały, że zawartość hydroksyproliny w grupie leczonej była wyraźnie wyższa w poszczególnych dniach następujących po powstaniu rany. Zawartość białka rosła stopniowo przez 10 dni. Obserwacje histopatologiczne wskazywały na umiarkowaną do intensywnej granulację tkanki w grupie leczonej 10. dnia. Na ich podstawie stwierdzono również, że nowa epiderma w grupie leczonej była grubsza niż w grupie kontrolnej po 16 dniach od zranienia. Prezentowane badania wskazują, że wyciąg etanolowy z C spinosa ma właściwości przyspieszające gojenie się ran.
Źródło:
Herba Polonica; 2015, 61, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial activity of Tribulus terrestris methanol extract against clinical isolates of Escherichia coli
Działanie antybakteryjne wyciągu etanolowego z Tribulus terrestris na kliniczne izolaty Escherichia coli
Autorzy:
Batoei, S.
Mahboubi, M.
Yari, R.
Powiązania:
https://bibliotekanauki.pl/articles/72055.pdf
Data publikacji:
2016
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Opis:
Introduction: Tribulus terrestris L. is traditionally used for treatment of urinary tract infections. Escherichia coli, as the most prominent agent of urinary tract infections, can be sensitive to T. terrestris extract. Objectives: The aim of this study was to evaluate the antibacterial activity of T. terrestris methanol extract against clinical isolates of E. coli from urinary tract infections. Saponins were determined as main constituents of T. terrestris methanol extract. Methods: The antibacterial activities of T. terrestris methanol extract were evaluated by micro-broth dilution assay. The synergistic effects of T. terrestris methanol extract were screened with gentamicin by micro titer plate and disc diffusion assays. The isobologram curve was figured and the Fractional Inhibitory Concentration Index (FICI) was determined. Results: The saponin content of T. terrestris methanol extract was 54% (w/w). The means of MIC and MBC values for E. coli clinical isolates (n=51) were 3.5±0.27 and 7.4±0.5 mg/ml while these amounts were 3.9±1.3 and 6.4±1.8 μg/ml for gentamicin. T. terrestris methanol extract and gentamicin had synergistic effect with FICI equal to 0.1375. Conclusion: Therefore, T. terrestris can be applicable as alternative treatment in management of urinary tract infections.
Wstęp: Tribulus terrestris L. jest tradycyjnie stosowany w leczeniu zakażeń układu moczowego. Escherichia coli, jako główny czynnik zakażeń tego układu, może wykazywać wrażliwość na działanie ekstraktu z T. terrestris.Cel: Celem badań było określenie aktywności przeciwbakteryjnej ekstraktu metanolowego uzyskanego z ziela T. terrestris w stosunku do szczepów E. coli izolowanych od osób z zakażeniami układu moczowego. Metody: Działanie przeciwbakteryjne ekstraktu metanolowego z T. terrestris określano metodą rozcieńczeń w pożywce płynnej. Działanie synergistyczne ekstraktu metanolowego z T. terrestris z gentamycyną badano metodą krążków bibułowych w podłożu agarowym. Na tej podstawie wyznaczono krzywą izobolograficzną oraz ułamkowe stężenie hamujace dla obu substancji (Fractional Inhibitory Concentration Index – FICI). Wyniki: Zawartość saponin w ekstrakcie metanolowym z T. terrestris wynosiła 54% (w/w). Średnie wartości MIC i MBC dla szczepów klinicznych E. coli (n=51) wynosiły odpowiednio 3,5±0,27, 7,4±0,5 mg/ml, a dla gentamycyny odpowiednio 3,9±1,3 i 6,4±1,8 µg/ml. Ekstrakt metanolowy i gentamycyna wykazywały działanie synergistyczne na poziomie FICI równego 0,1375. Wniosek: Wykazano, że ekstrakt metanolowy z ziela T. terrestris, może być stosowany jako alternatywny środek do leczenia zakażeń układu moczowego.
Źródło:
Herba Polonica; 2016, 62, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The expression level of a recombinant lipase predicted in silico by different codon optimization algorithms
Autorzy:
Bello, Adebayo J.
Igwo-Ezikpe, Miriam
Toye, Emuejevoke T.
Okpuzor, Joy
Powiązania:
https://bibliotekanauki.pl/articles/1046552.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
COOL
Codon optimization
Cohnella
Escherichia coli
ExpOptimizer
JCat
algorithm
in silico
lipase
presyndocon
protein expression
Opis:
Optimizing codons of gene of interest has offered a value-added way to increase heterologous expression of proteins. Different algorithms have been designed to achieve this purpose. These algorithms make use of parameters such as codon Adaptation index (CAI), codon context (CC), percentage guanine-cytosine (GC) content and RNA instability for predicting optimal codons responsible for increased protein expression. Lipase 3646 is an enzyme with great potential for industrial applications. This enzymes has been described to possess thermostable properties with relative stability in high alkaline pH and at different concentrations of organic solvents and inhibitors. This research therefore used JCat, Codon optimization online (COOL), presyndocon and ExpOptimizer algorithms to predict expression level of lipase 3646 enzyme in silico by optimizing its gene coding sequence. The results showed that there were variations in the CAI generated by the algorithms for the same 3646 DNA sequence which suggests that each algorithm is specific for its own generated CAI. COOL algorithm prediction based on other parameters showed good results for potential expression of the lipase. Thus, we recommend COOL algorithm for codon optimization of the lipase 3646 gene for industrial applications.
Źródło:
World Scientific News; 2019, 137; 31-41
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Guanylin and related peptides
Autorzy:
Beltowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/69588.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
gastrointestinal tract
lymphoguanylin
uroguanylin
peptide
guanylin
Escherichia coli
cystic fibrosis
protein kinase G
enterotoxin
diarrhea
Źródło:
Journal of Physiology and Pharmacology; 2001, 52, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of rpoS in the regulation of glyoxalase III in Escherichia coli.
Autorzy:
Benov, Ludmil
Sequeira, Fatima
Beema, Anees
Powiązania:
https://bibliotekanauki.pl/articles/1041571.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
methylglyoxal
stationary phase
glyoxalases
RpoS
Opis:
Methylglyoxal is an endogenous electrophile produced in Escherichia coli by the enzyme methylglyoxal synthase to limit the accumulation of phosphorylated sugars. In enteric bacteria methylglyoxal is detoxified by the glutathione-dependent glyoxalase I/II system, by glyoxalase III, and by aldehyde reductase and alcohol dehydrogenase. Here we demonstrate that glyoxalase III is a stationary-phase enzyme. Its activity reached a maximum at the entry into the stationary phase and remained high for at least 20 h. An rpoS- mutant displayed normal glyoxalase I and II activities but was unable to induce glyoxalase III in stationary phase. It thus appears that glyoxalase III is regulated by rpoS and might be important for survival of non-growing E. coli cultures.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 857-860
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sery dojrzewające jako środowisko rozwoju patogenów
Ripened cheeses as pathogens growth environment
Autorzy:
Berthold, A.
Pluta, A.
Dolińska, M.
Powiązania:
https://bibliotekanauki.pl/articles/228836.pdf
Data publikacji:
2008
Wydawca:
Wyższa Szkoła Menedżerska w Warszawie
Tematy:
sery podpuszczkowe
zatrucia pokarmowe
Liseria monocytogenes
Salmonella spp.
Escherichia coli
Staphylococcus aureus
rennet cheeses
foot poisonings
Opis:
Celem artykułu jest omówienie możliwości rozwoju w serach podpuszczkowych dojrzewających drobnoustrojów patogennych, takich jak: Listeria monocytogenes, Escherichia coli. Salmonella sp. oraz Staphylococcus aureus. Przeanalizowano wpływ różnych czynników technologicznych wpływających na rozwój patogenów w serach. Dokonano przeglądu piśmiennictwa dotyczącego jakości mikrobiologicznej serów rynkowych. Przedstawiono przypadki zatruć pokarmowych wywołanych spożyciem serów dojrzęwająacych, zanieczyszczonych drobnoustrojami chorobotwórczymi.
The aim of the study was to review the possibilities of growth in rennet cheeses pathogenic microorganisms, such as: Listeria monocytogenes, Escherichia coli, Salmonella sp. and Staphylococcus aureus. The influence of some factors on the growth of pathogenic bacteria was analysed. The review of the literature concerning the microbiological quality of market cheeses was made. The cases of food pisonings, caused by consumption of rennet cheeses, contaminated with pathogenic bacteria were shown.
Źródło:
Postępy Techniki Przetwórstwa Spożywczego; 2008, 1; 79-84
0867-793X
2719-3691
Pojawia się w:
Postępy Techniki Przetwórstwa Spożywczego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.
Autorzy:
Bicka, Leokadia
Kuźmak, Jacek
Kozaczyńska, Bożena
Płucienniczak, Andrzej
Skorupska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1044191.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
bovine leukemia virus
fusion protein p24
immunoblotting assay
gag gene cloning
Opis:
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 227-232
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Escherichia coli O104:H4 outbreak - have we learnt a lesson from it?
Autorzy:
Bloch, Sylwia
Felczykowska, Agnieszka
Nejman-Faleńczyk, Bożena
Powiązania:
https://bibliotekanauki.pl/articles/1039632.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
detection methods
Escherichia coli O104:H4
STEC
mobile genetic elements
outbreak
Opis:
Shiga toxin-producing Escherichia coli (STEC) strains belong to the group of pathogens that cause bloody diarrhea and hemorrhagic colitis with often severe complications. The main problem with human pathogenic E. coli strains, including STEC, is a wide spectrum of phenotypes and clinical manifestations. It is related to a variety of exchangeable genetic elements, like plasmids, bacteriophages, transposons and pathogenicity islands, that take part in horizontal gene transfer which influences creation of new dangerous bacterial strains. A good example of this phenomenon is a novel Shiga toxin-producing E. coli O104:H4 serotype that was associated with a widespread and severe foodborne disease outbreak in Germany in 2011. The O104:H4 strain was created by a number of horizontal gene transfer events between two distinct pathogens, resulting in the emergence of the new, atypical strain. That outbreak proved that also rare and unusual serotypes of STEC may be a significant risk factor and that the procedures recommended for STEC detection were not suitable to deal with this kind of pathogens. With respect to new combinations of chromosomal and extrachromosomal elements in susceptible bacterial hosts, epidemics and frequent human infections caused by STEC strains, we suggest that more attention should be paid to the development and improvement of diagnostic methods. It is difficult to determine STEC bacteria by general microbiological, biochemical and immunological assays, because strains can vary dramatically in their phenotypic and serotypic properties. It is postulated that standardized genetic tests, based on detection of features most frequently presented by STEC, particularly those located on easily exchangeable elements (such as Shiga toxin-encoding phages), can be more adequate for STEC detection.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 483-488
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 209

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies