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Tytuł:
β-Glucan-Mediated Alleviation of NaCl Stress in Ocimum basilicum L. in Relation to the Response of Antioxidant Enzymes and Assessment DNA Marker
Autorzy:
Alhasnawi, Arshad Naji
Powiązania:
https://bibliotekanauki.pl/articles/124951.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
NaCl-stress
beta-glucan
antioxidants
molecular markers
DNA variations
genetic stability
Opis:
Salinity is one of the most important abiotic stresses which can negatively affect the plant metabolic processes in the world. This can impact the plant production, either for economic or sustenance benefits. The salinity stress can cause many physiological and biochemical changes in the plants. β-glucans are important polysaccharides, which are present in the cell walls of various cereal grains. They protect the plant responses and occur in plant suspensions. In this study, the researchers attempted to investigate various physiological mechanisms and determine the role of the β-glucans in the NaCl-mediated stress conditions on the Ocimum basilicum L. seedlings. For this purpose, they carried out an experiment for assessing various shoot and root parameters along with the antioxidant enzyme activities, proline levels and the ISSR markers. When the seedlings were exposed to the NaCl stress conditions, they showed a significant decrease in the growth parameters and an increase in the antioxidant and proline levels compared to the control seedlings grown under normal saline conditions. On the other hand, the β-glucantreated seeds, when grown under the saline stress conditions, showed better growth parameters as well as high antioxidant enzyme activities and proline levels, compared to the control and NaCl-treated plants. Furthermore, a PCR analysis was carried out using the ISSR-marker technology, which could help in evaluating the DNA fingerprints and genetic variations in the plants. The results indicated that the exogenous application of the β-glucans could protect the antioxidant enzyme activities and protect the plants against the salinity stresses, without affecting the DNA-markers without affecting the genetic variations and could be a better choice for use in DNA-markers.
Źródło:
Journal of Ecological Engineering; 2019, 20, 8; 90-99
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metody AFLP do analizy DNA rzepaku ozimego.
Autorzy:
Matuszczak, M
Powiązania:
https://bibliotekanauki.pl/articles/833457.pdf
Data publikacji:
2002
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
rosliny oleiste
genetyka roslin
analiza DNA
markery molekularne
rzepak ozimy
oil plant
plant genetics
DNA analysis
molecular marker
winter rape
Źródło:
Rośliny Oleiste - Oilseed Crops; 2002, 23, 2; 255-265
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie markerów DNA w selekcji marchwi z cechą cytoplazmatycznej męskiej sterylności typu płatkowego
The use of DNA markers in the selection of carrot lines with cytoplasmic male sterility trait
Autorzy:
Szczechura, W.
Nowakowska, M.
Nowak, K.
Kamiński, P.
Nowak, R.
Kozik, E.U.
Powiązania:
https://bibliotekanauki.pl/articles/2119577.pdf
Data publikacji:
2019
Wydawca:
Instytut Ogrodnictwa
Tematy:
CMS
carrot
DNA markers
marker-assisted selection (MAS)
Opis:
Cytoplasmic male sterility (CMS) is a maternally inherited trait in which a plant is unable to produce functional pollen. It occurs in many plants, including carrots. The expression of this trait depends on environmental conditions, especially temperature – therefore, DNA markers become an excellent tool for selecting carrot genotypes with this feature. In this study, we used seventeen DNA markers available in the literature, linked with various mitochondrial DNA sequences that are associated with the CMS trait. Their amplification was tested on male sterile and male fertile plants. Only five markers – A321/392, I214/401/435, K751, N795, O790 – correctly identified the tested genotypes. They can be used as selection tools in carrot breeding programs.
Źródło:
Zeszyty Naukowe Instytutu Ogrodnictwa; 2019, 27; 53-62
2300-5882
2391-8969
Pojawia się w:
Zeszyty Naukowe Instytutu Ogrodnictwa
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of random amplified polymorphic DNA [RAPD] assay for differentiation among isolates of Stagonospora spp. and Septoria tritici
Autorzy:
Czembor, P C
Arseniuk, E
Powiązania:
https://bibliotekanauki.pl/articles/2047271.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Stagonospora
Stagonospora avenae
Septoria tritici
random amplified polymorphic DNA
pathogen
polymerase chain reaction
DNA
molecular marker
fungal isolate
Stagonospora nodorum
genetic variation
Opis:
The genetic similarity of three species: Septoria tritici, Stagonospora nodorum and Stagonospora avenae f. sp. triticea - important pathogens in many cereal production areas worldwide was assessed by random amplified polymorphic DNA (RAPD) assay. In preliminary research DNA of 14, 9, and 7 monopyenidios- pore isolates of S. nodorum, S. tritici, and S. a. tritícea, respectively, were amplified by PCR with four primers. Afterwards the research was focused on three mono- pyenidiospore isolates from each species studied. The isolates of each species selected for the study varied in pathogenicity and were diverse geographically. PCR with the set of 14 selected primers resulted in 99 different bands, ranged from 180 to 2500 base pairs in length. Most primers in PCR (especially RAD11, RAD31, RAD32, RAD33) revealed uniform bands for isolates of S. a. tritícea, that allow to identify this species among the others. The cluster analysis using Unweighed Pair-Group Method with Averaging (UPGMA) revealed interspecies disagreement among the isolates ranging from 32 to 53%. The intraspecies disagreement ranges were 17-20%, 38-43%, 42-44% for S. avenae f. sp. triticea, S. nodorum and S. tritici, respectively. Cluster analysis classified isolates into three homogeneous clusters. Each cluster grouped isolates of one species according to their current taxonomie ranks based on spore size, colony morphology and host ranges. In addition, two of the clusters represented by isolates of S. nodorum and S. a. tritícea were distinctly separated at a lower linkage distance from the third one comprising isolates of S. tritici. A slight inconsistency found in grouping some isolates indicates that such groupings should be done with caution. The present study indicates that the PCR- RAPD assay is of a potential use in taxonomy of Stagonospora spp. and Septoria tritici as well as in molecular identification of casual disease agents.
Źródło:
Journal of Applied Genetics; 1996, 37, 3; 239-251
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transformation of wild Solanum species resistant to late blight by using reporter gene gfp and msh2 genes
Autorzy:
Rakosy-Tican, Lenuta
Aurori, Adriana
Aurori, Cristian M.
Ispas, Gabriela
Famelaer, Ivan
Powiązania:
https://bibliotekanauki.pl/articles/2198986.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Agrobacterium tumefaciens mediated transformation
DNA mismatch repair
gfp
nptII marker gene
Opis:
Green fluorescent protein (gfp) reporter gene and nptII marker gene were used to optimize Agrobacterium tumefaciens (agro) mediated transformation of wild Solanum genotypes resistant to late blight. Different genotypes of Solanum bulbocastanum, S. chacoense, S. microdontum and S. verrucosum were assessed for their regeneration ability on MS based media and for agro-mediated transformation. As the first step reporter genes were used to optimize transformation protocol for each species and then the transfer of genes involved in mismatch repair of DNA were attempted in Solanum chacoense. For transformation, either leaf or stem fragments were used. It was shown that gfp is a valuable and elegant tool for monitoring the efficiency of transformation or the occurrence of chimera in all genotypes. Transformation efficiency was dependent on a plant genotype. A number of genotypes have been successfully transformed and they expressed constitutively the bright green fluorescence of gfp without any side effects. The most recalcitrant species proved to be S. microdontum, which did not regenerate plants although different media and phytohormones had been used. The best protocol for S. chacoense transformation was also found to work in the transfer of msh2 genes. Msh2 isolated from Arabidopsis was used and transferred either as mutated (Apa) or antisense (As) gene. The integration of msh2-mutated gene into S. chacoense genome was demonstrated by PCR amplification and confirmed by RT-PCR for some of the putative transgenic clones. The implications of mismatch repair in homologous recombination and its importance for potato improvement are discussed. 
Źródło:
Plant Breeding and Seed Science; 2004, 50; 119-127
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of bulk segregant analysis to identify a RAPD marker linked to the Mla locus of barley
Autorzy:
Czembor, Paweł Cz.
Czembor, Jerzy H.
Powiązania:
https://bibliotekanauki.pl/articles/2198932.pdf
Data publikacji:
2004-06-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
barley
Blumeria graminis f. sp. hordei
bulked segregant analysis
DNA marker
Mla locus
RAPD
Opis:
Resistance to powdery mildew, Blumeria graminis f.sp. hordei, is a major goal of many barley breeding programs. Resistance conferred by genes located at Mla locus is commonly used by barley breeders for effective control of powdery mildew. The use of molecular markers may facilitate barley breeding for powdery mildew resistance. In this study, bulked segregant analysis (BSA) was used to determine random amplified polymorphic DNAs (RAPDs) markers linked to Mla locus. Thirty one homozygous (17 resistant and 14 susceptible) F3 families from a cross between variety Pallas and single plant line E 1059-1-1 carrying gene at Mla locus were used as plant material. A total of 385 random 10-mer primers were screened to identify polymorphism between the appropriate resistant and susceptible DNA bulks and parents in BSA analysis. Only one PCR marker OPAA3400 (primer sequence: 5’-TTAGCGCCCC-3’), amplified in polymerase chain reaction (PCR) proved close linkage and was positioned in distance of 10 cM from Mla locus with 5.0 LOD threshold.
Źródło:
Plant Breeding and Seed Science; 2004, 49; 41-49
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The preliminary stage of SCAR markers development for Ranunculus subgen. Batrachium
Autorzy:
Jopek, M.
Powiązania:
https://bibliotekanauki.pl/articles/80542.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
preliminary stage
SCAR molecular marker
Batrachium
aquatic plant
Ranunculaceae
aquatic environment
DNA marker
internal transcribed spacer
random amplified polymorphic DNA
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
Autorzy:
Wachowiak, W
Baczkiewicz, A.
Celinski, K.
Prus-Glowacki, W.
Powiązania:
https://bibliotekanauki.pl/articles/41357.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Scotch pine
Pinus sylvestris
Pinus mugo
dwarf pine
hybridization
DNA marker
mtDNA
trnV-rbcL region
chloroplast
DNA polymorphism
Opis:
Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
Źródło:
Dendrobiology; 2004, 51; 67-72
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Somatic embryogenesis and genetic uniformity of regenerated cassava plants from low-temperature preserved secondary somatic cotyledons
Autorzy:
Opabode, J.T.
Ajibola, O.V.
Oyelakin, O.O.
Akinyemiju, O.A.
Powiązania:
https://bibliotekanauki.pl/articles/79828.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cassava
Manihot esculenta
somatic embryogenesis
plant regeneration
cotyledon
organogenesis
DNA extraction
RAPD marker
dehydration
low temperature
regeneration
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Śladami badań Władysława Jedlińskiego – określenie pochodzenia świerka na terenie Nadleśnictwa Skrwilno
Following the research of Wladyslaw Jedlinski – determination of the origin of spruce in the Skrwilno Forest District
Autorzy:
Lewandowski, A.
Litkowiec, M.
Fischer, A.
Powiązania:
https://bibliotekanauki.pl/articles/1008413.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Nadlesnictwo Skrwilno
drzewa lesne
swierk pospolity
Picea abies
pochodzenie roslin
markery genetyczne
DNA mitochondrialny
origin
picea abies
mitochondrial marker
Opis:
Origin of Norway spruce in the territory of Skrwilno Forest District was studied with the use of mitochondrial mt−D02 region inherited through maternal line. The former suppositions of Jedliński, who claimed that the origin of spruce in this territory was natural, have been confirmed. Concurrently, it has been established that in the majority of tree stands subject to our study there occurs spruce of Carpathian origin. The obtained results together with the palinological data indicate that the northern border of spruce range in Poland runs right through the territory of Skrwilno Forest District and not about 100 km to the south from this place as has been claimed so far.
Źródło:
Sylwan; 2012, 156, 09; 703-709
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rumex thyrsiflorus Fingerh. – sex ratios among seedlings and explants cultured in vitro
Autorzy:
Dziedzic, K.
Cygan, M.
Mizia, P.
Kwolek, D.
Slesak, H.
Powiązania:
https://bibliotekanauki.pl/articles/81088.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Rumex thyrsiflorus
Rumex acetosa
sex ratio
sex chromosome
seedling
explant
in vitro culture
chromosome
scanning electron microscopy
DNA marker
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Powdery mildew resistance genes in wheat: verification of STS markers
Autorzy:
Stepien, L
Chen, Y.
Chelkowski, J.
Kowalczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/2048311.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Aegilops speltoides
randomly amplified polymorphic DNA analysis
powdery mildew
wheat
wheat cultivar
Sequence Tagged Site marker
resistance gene
Pm gene
Źródło:
Journal of Applied Genetics; 2001, 42, 4; 413-423
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Postglacjalna migracja jodły pospolitej (Abies alba Mill.) do Polski - analiza na podstawie polimorfizmu mitochondrialnego DNA
Postglacial migration of silver fir (Abies alba Mill.) to Poland - analysis on the basis of mitochondrial DNA polymorphism
Autorzy:
Pawlaczyk, E. M.
Kroplewska, I.
Bobowicz, M. A.
Powiązania:
https://bibliotekanauki.pl/articles/991708.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
drzewa lesne
migracje roslin
okres polodowcowy
Polska
jodla pospolita
Picea abies
populacje roslin
badania molekularne
DNA mitochondrialny
polimorfizm DNA
silver fir (abies alba mill.)
refugium
postglacial migration
mitochondrial marker
Opis:
Mitochondrial DNA marker was applied to 10 populations of silver fir from Poland and one from Belarusia. These populations were located within and beyond the natural range of silver fir. The marker exhibited two highly conserved alleles (first – 230 bp and second – 150 bp) based on an insertion−deletion of 80 bp in the fourth intron of the mitochondrial nad5 gene. The geographical distribution of the maternally inherited mitochondrial variation is known to support the existence of at least two refugia with two recolonizing maternal lineages remaining largely separated throughout the range. Our results provide that in all studied populations the first allele was discovered. Therefore we postulate that the silver fir migrate to Poland from the refugium in western Europe (probably from central Italy).
Źródło:
Sylwan; 2013, 157, 06; 458-463
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pochodzenie świerka pospolitego (Picea abies (L.) Karst.) w Nadleśnictwie Kartuzy
Origin of Norway spruce (Picea abies (L.) Karst.) in the Kartuzy Forest District
Autorzy:
Lewandowski, A.
Szydlarski, M.
Litkowiec, M.
Powiązania:
https://bibliotekanauki.pl/articles/989990.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Nadlesnictwo Kartuzy
drzewa lesne
swierk pospolity
Picea abies
pochodzenie roslin
badania genetyczne
markery genetyczne
DNA mitochondrialny
origin
picea abies
mitochondrial marker
mitotype
Opis:
The origin of the Norway spruce in the Kartuzy Forest District was verified using the maternally inherited mitochondrial DNA marker mt15−D02 that shows distinct geographical distribution in Europe. We analyzed 462 trees from 26 populations. The majority of trees were of Alpine origin and they were present in all populations at a frequency of 6−90% (mean 59%). About 30% of trees were of the Carpathian origin and the least abundant (11%) were trees of north−eastern origin. The trees carrying north−eastern type of the mitochondial DNA marker were found only in 13 populations, but at a very different frequency (from 5 to 88%). The possibility of natural origin of Norway spruce in the area of Kartuzy Forest District was shortly discussed.
Źródło:
Sylwan; 2014, 158, 07; 509-515
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pochodzenie drzewostanów świerkowych (Picea abies L. Karst.) z Puszczy Białowieskiej na tle regionu RDLP Białystok na podstawie analiz mitochondrialnego DNA
Origin of the Norway spruce (Picea abies L. Karst.) stands from Bialowieza Forest at the level of Bialystok Regional Directorate of the State Forests based on mitochondrial DNA analysis
Autorzy:
Nowakowska, J.A.
Łukaszewicz, J.
Borys, M.
Tereba, A.
Konecka, A.
Zawadzka, A.
Sułkowska, M.
Zajączkowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/987121.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Puszcza Bialowieska
drzewostany swierkowe
drzewa lesne
swierk pospolity
Picea abies
markery genetyczne
DNA mitochondrialny
pochodzenie roslin
zmiennosc genetyczna
RDLP Bialystok
genetic diversity
mitochondrial dna marker
postglacial migration routes
białowieża forest
Opis:
Considered to be the most natural of all other Polish stands Picea abies in Białowieża Forest has suffered severely of bark beetle damages for decades. In order to find out some historical events in native Norway spruce stands diversity at regional level, we carried out the study on poly− morphism with DNA markers and performed Principal Coordinate Analysis (PCoA) on the basis of genetic distance matrix. Two ranges (i.e. Baltico−Nordic and Hercyno−Carpathian) of Norway spruce cover north−eastern and southern Poland, respectively. The spruceless zone separates the these two ranges in lowland Poland. From genetic point of view, the Baltico−Nordic P. abies populations mainly harbor mitochondrial haplotype ‘c', whereas the spruces from the Hercyno− −Carpathian range – haplotype ‘a'. Until recently, the Białowieża Forest has been considered to belong to the northern range of spruce occurrence in Europe. Seven populations from Biało− wieża Forest were studied (two of them from the Strict Reserve in Białowieski National Park) according to mitochondrial DNA diversity, and compared with genetic diversity found in 24 other populations located in Białystok Regional Directorate of the State Forests (north−eastern Poland). All studied spruce populations were more than 100 years old and were of natural origin. As result, the spruces from the Białowieża Forest harbored three mitochondrial haplotypes of the nad1 gene (‘c', ‘a' and ‘a1'), with the haplotype ‘a' being more frequently present (>51%) than the hap− lotype ‘c'. The spruce stands from Białowieża Forest have the highest gene diversity (h Nei=0.527) comparing to the other stands from the Białystok RDSF. The PCoA proved the particularity of the spruces from Białowieża Forest grouping them into one cluster of genetic similarity. Our data demonstrated that most of Norway spruces populations from Białystok RDSF harbor haplotype ‘c', which confirms their historical relationship with the Baltico−Nordic range of P. abies in Europe, while most spruces from Białowieża Forest have different historic origin, because they share high frequency of the southern haplotype ‘a'. It also turned out that Norway spruce pop− ulations from two different European ranges met in lowland Poland after the last glacial period, as proved by the presence of two mitochondrial haplotypes ‘a' and ‘c'. It can be assumed, that the spruceless zone was created by human activity in the past millennia. The results of all molecular analyses confirmed the unique character of spruces of Białowieża Forest, which is distinguished by the greater richness of the gene pool in comparison to the region of Białystok RDSF as well as good adaptation to local environmental conditions.
Źródło:
Sylwan; 2017, 161, 01; 40-51
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł

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