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Wyszukujesz frazę "DNA marker" wg kryterium: Wszystkie pola


Tytuł:
Frequent D-loop polymorphism in mtDNA enables genotyping of 1400-year-old human remains from Merowingian graves
Autorzy:
Zeller, M
Mirghomizadeh, F.
Wehner, H.D.
Blin, N.
Powiązania:
https://bibliotekanauki.pl/articles/2042042.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
X chromosome
ancient remains
amplification technique
Y chromosome
ancient DNA
mtDNA
remains
Merowingian culture
polymorphism
mitochondrial DNA
man
DNA marker
DNA extraction
DNA
Źródło:
Journal of Applied Genetics; 2000, 41, 4; 285-292
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isozyme and RAPD markers for the identification of pea, field bean and lupin cultivars
Autorzy:
Wolko, B
Swiecicki, W.K.
Kruszka, K.
Irzykowska, L.
Powiązania:
https://bibliotekanauki.pl/articles/2043430.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Lupinus angustifolius
legume crop
isoenzyme
morphological marker
electrophoresis
breeding selection
allozyme
Lupinus albus
seed
lupin cultivar
pea cultivar
field bean
polymorphism
Lupinus luteus
germ plasm
Vicia faba var.minor
enzyme system
cultivar identification
DNA
Pisum sativum
Źródło:
Journal of Applied Genetics; 2000, 41, 3; 151-165
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
Autorzy:
Wachowiak, W
Baczkiewicz, A.
Celinski, K.
Prus-Glowacki, W.
Powiązania:
https://bibliotekanauki.pl/articles/41357.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Scotch pine
Pinus sylvestris
Pinus mugo
dwarf pine
hybridization
DNA marker
mtDNA
trnV-rbcL region
chloroplast
DNA polymorphism
Opis:
Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
Źródło:
Dendrobiology; 2004, 51; 67-72
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie markerów DNA w selekcji marchwi z cechą cytoplazmatycznej męskiej sterylności typu płatkowego
The use of DNA markers in the selection of carrot lines with cytoplasmic male sterility trait
Autorzy:
Szczechura, W.
Nowakowska, M.
Nowak, K.
Kamiński, P.
Nowak, R.
Kozik, E.U.
Powiązania:
https://bibliotekanauki.pl/articles/2119577.pdf
Data publikacji:
2019
Wydawca:
Instytut Ogrodnictwa
Tematy:
CMS
carrot
DNA markers
marker-assisted selection (MAS)
Opis:
Cytoplasmic male sterility (CMS) is a maternally inherited trait in which a plant is unable to produce functional pollen. It occurs in many plants, including carrots. The expression of this trait depends on environmental conditions, especially temperature – therefore, DNA markers become an excellent tool for selecting carrot genotypes with this feature. In this study, we used seventeen DNA markers available in the literature, linked with various mitochondrial DNA sequences that are associated with the CMS trait. Their amplification was tested on male sterile and male fertile plants. Only five markers – A321/392, I214/401/435, K751, N795, O790 – correctly identified the tested genotypes. They can be used as selection tools in carrot breeding programs.
Źródło:
Zeszyty Naukowe Instytutu Ogrodnictwa; 2019, 27; 53-62
2300-5882
2391-8969
Pojawia się w:
Zeszyty Naukowe Instytutu Ogrodnictwa
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Powdery mildew resistance genes in wheat: verification of STS markers
Autorzy:
Stepien, L
Chen, Y.
Chelkowski, J.
Kowalczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/2048311.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Aegilops speltoides
randomly amplified polymorphic DNA analysis
powdery mildew
wheat
wheat cultivar
Sequence Tagged Site marker
resistance gene
Pm gene
Źródło:
Journal of Applied Genetics; 2001, 42, 4; 413-423
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia
Autorzy:
Sawicki, J.
Szczecinska, M.
Powiązania:
https://bibliotekanauki.pl/articles/57748.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Acutifolia
random amplified polymorphic DNA
Sphagnum
genetic similarity
molecular identification
molecular marker
polymerase chain reaction
genetic relationship
species identification
peat moss
chloroplast
nuclear genome
Opis:
RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.
Źródło:
Acta Societatis Botanicorum Poloniae; 2011, 80, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA fingerprints generated by R.18.1 DNA probe in two stocks of chickens: Green Legged Patridgenous [GLP] and Rhode Island Red [RIR]
Autorzy:
Rosochacki, S J
Hillel, J
Jaszczak, K
Zawadzka, M
Powiązania:
https://bibliotekanauki.pl/articles/2046824.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
multi-locus DNA probe
hybridization
animal breeding
poultry
molecular marker
DNA
chicken
Opis:
Hybridization of a multi-locus DNA probe, R 18.1, to genomic DNA from poultry showed a highly polymorphic fingerprint pattern. The detected DNA fingerprints are individually specific and differ between Green Legged Patridgenous (GLP) and Rhode Island Red (RIR) stock of chickens. The average numbers of detected bands in RIR were 18.68 and in GLP - 15.33, but the average band sharing levels were 0.619 and 0.431, respectively. The level of polymorphism may be connected possibly with a higher level of inbreeding in the examined stock of chicken.
Źródło:
Journal of Applied Genetics; 1997, 38, 2; 173-178
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Transformation of wild Solanum species resistant to late blight by using reporter gene gfp and msh2 genes
Autorzy:
Rakosy-Tican, Lenuta
Aurori, Adriana
Aurori, Cristian M.
Ispas, Gabriela
Famelaer, Ivan
Powiązania:
https://bibliotekanauki.pl/articles/2198986.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Agrobacterium tumefaciens mediated transformation
DNA mismatch repair
gfp
nptII marker gene
Opis:
Green fluorescent protein (gfp) reporter gene and nptII marker gene were used to optimize Agrobacterium tumefaciens (agro) mediated transformation of wild Solanum genotypes resistant to late blight. Different genotypes of Solanum bulbocastanum, S. chacoense, S. microdontum and S. verrucosum were assessed for their regeneration ability on MS based media and for agro-mediated transformation. As the first step reporter genes were used to optimize transformation protocol for each species and then the transfer of genes involved in mismatch repair of DNA were attempted in Solanum chacoense. For transformation, either leaf or stem fragments were used. It was shown that gfp is a valuable and elegant tool for monitoring the efficiency of transformation or the occurrence of chimera in all genotypes. Transformation efficiency was dependent on a plant genotype. A number of genotypes have been successfully transformed and they expressed constitutively the bright green fluorescence of gfp without any side effects. The most recalcitrant species proved to be S. microdontum, which did not regenerate plants although different media and phytohormones had been used. The best protocol for S. chacoense transformation was also found to work in the transfer of msh2 genes. Msh2 isolated from Arabidopsis was used and transferred either as mutated (Apa) or antisense (As) gene. The integration of msh2-mutated gene into S. chacoense genome was demonstrated by PCR amplification and confirmed by RT-PCR for some of the putative transgenic clones. The implications of mismatch repair in homologous recombination and its importance for potato improvement are discussed. 
Źródło:
Plant Breeding and Seed Science; 2004, 50; 119-127
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molekularna identyfikacja gatunków z rodzaju Abies na podstawie polimorfizmu DNA mitochondrialnego
Molecular identification of species from Abies genus based on the mitochondrial DNA polymorphism
Autorzy:
Pawlaczyk, E.M.
Staniak, J.
Maliński, T.
Bobowicz, M.A.
Powiązania:
https://bibliotekanauki.pl/articles/989708.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
drzewa lesne
genetyka roslin
jodla
Abies
gatunki roslin
identyfikacja
haplotypy
DNA mitochondrialny
polimorfizm DNA
abies species
haplotype
capillary electrophoresis
mitochondrial marker
Opis:
The plant material was collected on 34 individuals growing in the Dendrological Garden of Poznań University of Life Sciences (52°25'32,95" N 16°53'39,83" E) and Botanical Garden of Adam Mickiewicz University in Poznań (52°25'11,70" N 16°52'55,07" E). The species for this study originated from Europe, Asia Minor, central and eastern Asia and North America and included: Abies alba, Abies cephalonica, Abies cilicica, Abies equi−trojani, Abies sibirica, Abies koreana, Abies pinsapo, Abies ×insignis, Abies bornmulleriana, Abies homolepsis, Abies holophylla, Abies grandis, Abies concolor, Abies concolor var. violacea, Abies concolor var. lowiana, Abies nordmanniana, Abies ×arnoldiana, Abies nephrolepis and Abies balsamea. The aim of this study was to define the species haplotypes (the length of allele) on the basis of nad5−4 mitochondrial DNA marker detected by capillary electrophoresis. This marker has been suggested as an easy−to−use tool to distinguish species of the Abies genus and it could be species−specific. Seven different haplotypes were identified. The first one appears in the species from Europe, Asia and North America. The second one was detected in firs from Europe and Asia Minor. A. cephalonica and A. sibirica were identified by the third haplotype, which occurs also in A. alba from the Balkan region. The fourth haplotype is characteristic for species from Asia and North America. The fifth and sixth haplotypes were identified in A. pinsapo and A. numidica. The seventh haplotype was detected only in A. holophylla. Applied marker is a very useful for verification of fir species especially allopatric species, less for parapatric ones. This marker is more helpful to exclude the species than to precisely identify them.
Źródło:
Sylwan; 2015, 159, 08; 675-683
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Postglacjalna migracja jodły pospolitej (Abies alba Mill.) do Polski - analiza na podstawie polimorfizmu mitochondrialnego DNA
Postglacial migration of silver fir (Abies alba Mill.) to Poland - analysis on the basis of mitochondrial DNA polymorphism
Autorzy:
Pawlaczyk, E. M.
Kroplewska, I.
Bobowicz, M. A.
Powiązania:
https://bibliotekanauki.pl/articles/991708.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
drzewa lesne
migracje roslin
okres polodowcowy
Polska
jodla pospolita
Picea abies
populacje roslin
badania molekularne
DNA mitochondrialny
polimorfizm DNA
silver fir (abies alba mill.)
refugium
postglacial migration
mitochondrial marker
Opis:
Mitochondrial DNA marker was applied to 10 populations of silver fir from Poland and one from Belarusia. These populations were located within and beyond the natural range of silver fir. The marker exhibited two highly conserved alleles (first – 230 bp and second – 150 bp) based on an insertion−deletion of 80 bp in the fourth intron of the mitochondrial nad5 gene. The geographical distribution of the maternally inherited mitochondrial variation is known to support the existence of at least two refugia with two recolonizing maternal lineages remaining largely separated throughout the range. Our results provide that in all studied populations the first allele was discovered. Therefore we postulate that the silver fir migrate to Poland from the refugium in western Europe (probably from central Italy).
Źródło:
Sylwan; 2013, 157, 06; 458-463
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Somatic embryogenesis and genetic uniformity of regenerated cassava plants from low-temperature preserved secondary somatic cotyledons
Autorzy:
Opabode, J.T.
Ajibola, O.V.
Oyelakin, O.O.
Akinyemiju, O.A.
Powiązania:
https://bibliotekanauki.pl/articles/79828.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cassava
Manihot esculenta
somatic embryogenesis
plant regeneration
cotyledon
organogenesis
DNA extraction
RAPD marker
dehydration
low temperature
regeneration
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Description of DNA analysis techniques and their application in oat (Avena L.) genome research
Charakterystyka technik analiz DNA oraz ich wykorzystanie w badaniach owsa (Avena L.)
Autorzy:
Okon, S.
Kowalczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/26845.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
DNA analysis technique
application
oat
Avena
genome
molecular marker
crossbreeding efficiency
genetic map
hexaploid
diploid
plant species
crown rust
powdery mildew
plant resistance
DNA marker
polymerase chain reaction
Opis:
DNA markers are used not only to estimate genetic similarity and distance but also to select and identify desirable forms, to assess the adjustment of breeding material, to confirm crossbreeding efficiency, to determine seed purity, and to identify the genes which determine important functional traits. In the case of oat, DNA markers were used to construct and increase the density of genetic maps both in hexaploid and diploid species. The development of markers for some important traits provides a fast selection of genotypes containing dwarf genes as well as the resistance genes to crown rust and powdery mildew. Numerous analyses of genetic similarity between different species belonging to the genus Avena which are currently carried out may contribute to explaining the process of evolution within this genus and may also explain the development of particular species of oat.
Markery DNA znalazły zastosowanie nie tylko w ocenie podobieństwa lub dystansu genetycznego, ale również w selekcji i identyfikacji pożądanych form, ocenie wyrównania materiałów hodowlanych, potwierdzaniu skuteczności krzyżowań, ocenie czystości materiału siewnego czy też do identyfikacji genów, warunkujących ważne cechy użytkowe. U owsa posłużyły one między innymi do konstrukcji i zagęszczenia map genetycznych zarówno gatunków heksaploidalnych jak i diploidalnych. Opracowanie markerów dla niektórych cech użytkowych pozwala na szybką selekcję genotypów zawierających geny karłowatości, odporności na rdzę koronową czy mączniaka prawdziwego. Natomiast prowadzone liczne analizy podobieństwa genetycznego różnych gatunków z rodzaju Avena mogą przyczynić się do wyjaśnienia ewolucji w obrębie tego rodzaju jak również mogą wyjaśnić powstawanie poszczególnych gatunków owsa.
Źródło:
Acta Agrobotanica; 2012, 65, 1
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers
Analiza zróżnicowania genetycznego wśród genotypów Arnica montana L. za pomocą markerów RAPD
Autorzy:
Okoń, S.
Paczos-Grzęda, E.
Łoboda, M.
Sugier, D.
Powiązania:
https://bibliotekanauki.pl/articles/11542962.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
DNA polymorphism
identification
genetic diversity
Arnica montana
genotype
RAPD marker
medicinal plant
molecular analysis
Opis:
Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.
Arnica montana L. jest jedną z najcenniejszych roślin zielarskich wykorzystywanych w medycynie, farmacji i przemyśle kosmetycznym. W dostępnej literaturze liczba doniesień związanych z analizą molekularną arniki jest znikoma, dlatego też celem prezentowanych badań była optymalizacja procesu izolacji DNA ze świeżych liści oraz identyfikacja zróżnicowania genetycznego oparta na markerach RAPD. W prezentowanej pracy w celu uzyskania czystego DNA do izolacji wykorzystano zestaw DNA Plant & Fungi DNA Purification Kit (Euro) oraz oczyszczanie za pomocą długiej elektroforezy w żelu agarozowym. Spośród testowanych 40 starterów RPAD do analiz wybrano 12 generujących stabilne i polimorficzne wzory prążków. Wyselekcjonowane startery amplifikowały 120 fragmentów, spośród których 111 (92,5%) było polimorficznych. Wykorzystujac markery RAPD utworzono matryce podobieństwa genetycznego. średnia wartość podobieństwa analizowanych genotypów wynosiła 0.886. Najwyższy współczynnik podobieństwa genetycznego oszacowano pomiędzy genotypami GA17 i GA18, które ulokowały się blisko siebie na uzyskanym dendrogramie.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 63-71
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of DNA markers against illegal logging as a new tool for the Forest Guard Service
Autorzy:
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/38611.pdf
Data publikacji:
2011
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
application
DNA marker
DNA structure
wood
molecular identification
Forest Guard Service
tree species
determination
DNA profile
polymerase chain reaction
Opis:
DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2011, 53, 2
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pochodzenie drzewostanów świerkowych (Picea abies L. Karst.) z Puszczy Białowieskiej na tle regionu RDLP Białystok na podstawie analiz mitochondrialnego DNA
Origin of the Norway spruce (Picea abies L. Karst.) stands from Bialowieza Forest at the level of Bialystok Regional Directorate of the State Forests based on mitochondrial DNA analysis
Autorzy:
Nowakowska, J.A.
Łukaszewicz, J.
Borys, M.
Tereba, A.
Konecka, A.
Zawadzka, A.
Sułkowska, M.
Zajączkowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/987121.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Puszcza Bialowieska
drzewostany swierkowe
drzewa lesne
swierk pospolity
Picea abies
markery genetyczne
DNA mitochondrialny
pochodzenie roslin
zmiennosc genetyczna
RDLP Bialystok
genetic diversity
mitochondrial dna marker
postglacial migration routes
białowieża forest
Opis:
Considered to be the most natural of all other Polish stands Picea abies in Białowieża Forest has suffered severely of bark beetle damages for decades. In order to find out some historical events in native Norway spruce stands diversity at regional level, we carried out the study on poly− morphism with DNA markers and performed Principal Coordinate Analysis (PCoA) on the basis of genetic distance matrix. Two ranges (i.e. Baltico−Nordic and Hercyno−Carpathian) of Norway spruce cover north−eastern and southern Poland, respectively. The spruceless zone separates the these two ranges in lowland Poland. From genetic point of view, the Baltico−Nordic P. abies populations mainly harbor mitochondrial haplotype ‘c', whereas the spruces from the Hercyno− −Carpathian range – haplotype ‘a'. Until recently, the Białowieża Forest has been considered to belong to the northern range of spruce occurrence in Europe. Seven populations from Biało− wieża Forest were studied (two of them from the Strict Reserve in Białowieski National Park) according to mitochondrial DNA diversity, and compared with genetic diversity found in 24 other populations located in Białystok Regional Directorate of the State Forests (north−eastern Poland). All studied spruce populations were more than 100 years old and were of natural origin. As result, the spruces from the Białowieża Forest harbored three mitochondrial haplotypes of the nad1 gene (‘c', ‘a' and ‘a1'), with the haplotype ‘a' being more frequently present (>51%) than the hap− lotype ‘c'. The spruce stands from Białowieża Forest have the highest gene diversity (h Nei=0.527) comparing to the other stands from the Białystok RDSF. The PCoA proved the particularity of the spruces from Białowieża Forest grouping them into one cluster of genetic similarity. Our data demonstrated that most of Norway spruces populations from Białystok RDSF harbor haplotype ‘c', which confirms their historical relationship with the Baltico−Nordic range of P. abies in Europe, while most spruces from Białowieża Forest have different historic origin, because they share high frequency of the southern haplotype ‘a'. It also turned out that Norway spruce pop− ulations from two different European ranges met in lowland Poland after the last glacial period, as proved by the presence of two mitochondrial haplotypes ‘a' and ‘c'. It can be assumed, that the spruceless zone was created by human activity in the past millennia. The results of all molecular analyses confirmed the unique character of spruces of Białowieża Forest, which is distinguished by the greater richness of the gene pool in comparison to the region of Białystok RDSF as well as good adaptation to local environmental conditions.
Źródło:
Sylwan; 2017, 161, 01; 40-51
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł

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