Wszystkie pola: DNA marker - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: β-Glucan-Mediated Alleviation of NaCl Stress in Ocimum basilicum L. in Relation to the Response of Antioxidant Enzymes and Assessment DNA Marker
Autorzy:: Alhasnawi, Arshad Naji
Powiązania:: https://bibliotekanauki.pl/articles/124951.pdf
Data publikacji:: 2019
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: NaCl-stress
beta-glucan
antioxidants
molecular markers
DNA variations
genetic stability
Opis:: Salinity is one of the most important abiotic stresses which can negatively affect the plant metabolic processes in the world. This can impact the plant production, either for economic or sustenance benefits. The salinity stress can cause many physiological and biochemical changes in the plants. β-glucans are important polysaccharides, which are present in the cell walls of various cereal grains. They protect the plant responses and occur in plant suspensions. In this study, the researchers attempted to investigate various physiological mechanisms and determine the role of the β-glucans in the NaCl-mediated stress conditions on the Ocimum basilicum L. seedlings. For this purpose, they carried out an experiment for assessing various shoot and root parameters along with the antioxidant enzyme activities, proline levels and the ISSR markers. When the seedlings were exposed to the NaCl stress conditions, they showed a significant decrease in the growth parameters and an increase in the antioxidant and proline levels compared to the control seedlings grown under normal saline conditions. On the other hand, the β-glucantreated seeds, when grown under the saline stress conditions, showed better growth parameters as well as high antioxidant enzyme activities and proline levels, compared to the control and NaCl-treated plants. Furthermore, a PCR analysis was carried out using the ISSR-marker technology, which could help in evaluating the DNA fingerprints and genetic variations in the plants. The results indicated that the exogenous application of the β-glucans could protect the antioxidant enzyme activities and protect the plants against the salinity stresses, without affecting the DNA-markers without affecting the genetic variations and could be a better choice for use in DNA-markers.
Źródło:: Journal of Ecological Engineering; 2019, 20, 8; 90-99
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: The use of bulk segregant analysis to identify a RAPD marker linked to the Mla locus of barley
Autorzy:: Czembor, Paweł Cz.
Czembor, Jerzy H.
Powiązania:: https://bibliotekanauki.pl/articles/2198932.pdf
Data publikacji:: 2004-06-20
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: barley
Blumeria graminis f. sp. hordei
bulked segregant analysis
DNA marker
Mla locus
RAPD
Opis:: Resistance to powdery mildew, Blumeria graminis f.sp. hordei, is a major goal of many barley breeding programs. Resistance conferred by genes located at Mla locus is commonly used by barley breeders for effective control of powdery mildew. The use of molecular markers may facilitate barley breeding for powdery mildew resistance. In this study, bulked segregant analysis (BSA) was used to determine random amplified polymorphic DNAs (RAPDs) markers linked to Mla locus. Thirty one homozygous (17 resistant and 14 susceptible) F3 families from a cross between variety Pallas and single plant line E 1059-1-1 carrying gene at Mla locus were used as plant material. A total of 385 random 10-mer primers were screened to identify polymorphism between the appropriate resistant and susceptible DNA bulks and parents in BSA analysis. Only one PCR marker OPAA3400 (primer sequence: 5’-TTAGCGCCCC-3’), amplified in polymerase chain reaction (PCR) proved close linkage and was positioned in distance of 10 cM from Mla locus with 5.0 LOD threshold.
Źródło:: Plant Breeding and Seed Science; 2004, 49; 41-49
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Evaluation of anthropogenic pollution in river water based on the genetic diversity of Aeromonas Hydrophila
Ocena zanieczyszczenia antropogenicznego wody rzecznej na podstwawie zróżnicowania genetycznego Aeromonas Hydrophila
Autorzy:: Korzekwa, K.
Gołaś, I.
Harnisz, M.
Powiązania:: https://bibliotekanauki.pl/articles/204566.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: woda rzeczna
zanieczyszczenie antropogeniczne
zróżnicowanie genetyczne
marker wielolekooporności
Aeromonas hydrophila
river water
aquaculture
DNA marker
genetic diversity
Opis:: Aeromonas hydrophila is a valuable indicator of the quality of water polluted by sewage and pathogens that pose a risk for humans and cold-blooded animals, including fi sh. The main aim of this research was to evaluate anthropogenic pollution of river water based on genetic diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR (ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A. hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi random and rep-BOX). None of the analyzed electrophoretic patterns was identical, implying that there were many sources of strain transmission. The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains, and drug resistance patterns for tetracycline, enrofl oxacin and erythromycin were determined. The most diverse A. hydrophila strains isolated from river water were susceptible to enrofl oxacine (HS = 0.27), whereas less diverse strains were susceptible to erythromycin (HS = 0.24). The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus) in the examined strains (resistant to three analyzed drugs) indicates that intensive fi sh cultivation affects the microbiological quality of river water.
Aeromonas hydrophila jest cennym wskaźnikiem jakości wody w przypadku zanieczyszczeń ściekami oraz mikroorganizmami względnie patogennymi dla człowieka i zwierząt zimnokrwistych, w tym ryb. Celem niniejszych badań była ocena zanieczyszczenia antropogenicznego na podstawie zróżnicowania genetycznego 82 szczepów A. hydrophila poprzez analizy RAPD, pół-przypadkowo amplifi kowanej klasy AP-PCR (ISJ) i konserwatywnego powtórzenia rep-BOX. Zróżnicowanie genetyczne A. hydrophila wyniosło HT = 0,28 (SD = 0,02) dla wszystkich markerów DNA (RAPD, pół-przypadkowe i rep-BOX). Wszystkie szczepy dla wszystkich markerów ujawniły indywidualny wzór elektroforetyczny, nie ujawniono jednego źródła rozprzestrzeniania się szczepów. U szczepów potwierdzono obecność genów aerolizyny (aerA), hemolizyny (ahh1) i kompleksu enzymów cytotoksycznych (AHCYTOGEN), jak również określono wzorzec oporności na tetracyklinę, enrofl oksacynę i erytromycynę. Najbardziej zróżnicowane okazały się szczepy A. hydrophila wrażliwe na enrofl oksacynę (HS = 0,27) a najmniej zróżnicowane były szczepy wrażliwe na erytromycynę (HS = 0,24). Wyselekcjonowany marker wielolekooporności (locus ISJ4-25, 1100 pz) obecny u szczepów (opornych na 3 rozpatrywane leki) świadczy o wpływie intensywnej hodowli ryb na jakość mikrobiologiczną wody rzecznej.
Źródło:: Archives of Environmental Protection; 2012, 38, 3; 41-50
2083-4772
2083-4810
Pojawia się w:: Archives of Environmental Protection
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Molecular identification of blast resistance genes in rice genotypes using gene-specific markers
Autorzy:: Al-Daej, M.I.
Ismail, M.
Rezk, A.A.
El-Malky, M.M.
Powiązania:: https://bibliotekanauki.pl/articles/80189.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: molecular identification
resistance gene
rice genotype
Oryza sativa
DNA marker
single-nucleotide polymorphism
simple sequence repeat
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Application of DNA markers against illegal logging as a new tool for the Forest Guard Service
Autorzy:: Nowakowska, J.A.
Powiązania:: https://bibliotekanauki.pl/articles/38611.pdf
Data publikacji:: 2011
Wydawca:: Instytut Badawczy Leśnictwa
Tematy:: application
DNA marker
DNA structure
wood
molecular identification
Forest Guard Service
tree species
determination
DNA profile
polymerase chain reaction
Opis:: DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).
Źródło:: Folia Forestalia Polonica. Series A . Forestry; 2011, 53, 2
0071-6677
Pojawia się w:: Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
Autorzy:: Wachowiak, W
Baczkiewicz, A.
Celinski, K.
Prus-Glowacki, W.
Powiązania:: https://bibliotekanauki.pl/articles/41357.pdf
Data publikacji:: 2004
Wydawca:: Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:: Scotch pine
Pinus sylvestris
Pinus mugo
dwarf pine
hybridization
DNA marker
mtDNA
trnV-rbcL region
chloroplast
DNA polymorphism
Opis:: Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
Źródło:: Dendrobiology; 2004, 51; 67-72
1641-1307
Pojawia się w:: Dendrobiology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: The preliminary stage of SCAR markers development for Ranunculus subgen. Batrachium
Autorzy:: Jopek, M.
Powiązania:: https://bibliotekanauki.pl/articles/80542.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
preliminary stage
SCAR molecular marker
Batrachium
aquatic plant
Ranunculaceae
aquatic environment
DNA marker
internal transcribed spacer
random amplified polymorphic DNA
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Rumex thyrsiflorus Fingerh. – sex ratios among seedlings and explants cultured in vitro
Autorzy:: Dziedzic, K.
Cygan, M.
Mizia, P.
Kwolek, D.
Slesak, H.
Powiązania:: https://bibliotekanauki.pl/articles/81088.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
Rumex thyrsiflorus
Rumex acetosa
sex ratio
sex chromosome
seedling
explant
in vitro culture
chromosome
scanning electron microscopy
DNA marker
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Frequent D-loop polymorphism in mtDNA enables genotyping of 1400-year-old human remains from Merowingian graves
Autorzy:: Zeller, M
Mirghomizadeh, F.
Wehner, H.D.
Blin, N.
Powiązania:: https://bibliotekanauki.pl/articles/2042042.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: X chromosome
ancient remains
amplification technique
Y chromosome
ancient DNA
mtDNA
remains
Merowingian culture
polymorphism
mitochondrial DNA
man
DNA marker
DNA extraction
DNA
Źródło:: Journal of Applied Genetics; 2000, 41, 4; 285-292
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Genetic variation of Picea abies in southern Germany as determined using isozyme and STS markers
Autorzy:: Konnert, M
Powiązania:: https://bibliotekanauki.pl/articles/40969.pdf
Data publikacji:: 2009
Wydawca:: Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:: international conference
Europe
forest ecosystem
plant breeding
tree
Norway spruce
Picea abies
genetic variation
Germany
isoenzyme
Bavaria
DNA marker
plant genetics
provenance
Opis:: Over 50 populations of Norway spruce from Bavaria were analysed at 23 isozyme gene loci. The mean genetic distances between these populations were quite small. A geographical grouping could not be observed, and discrimination between provenances from high and low altitudes was not identifiable using this marker type, either. The only difference between spruce populations from South Bavaria and those from Northeast Bavaria is in the presence of some distinct rare alleles. The highest values for the genetic diversity were detected for spruce stands in Northeast Bavaria (Frankonian Forest). Using STS markers, further genes of the nuclear genome of Picea abies can be dealt with. The genetic differences found on the basis of ten STS markers between different Picea abies seed lots and/or seedling populations are generally 2-3 times greater than those found by means of isozyme gene markers. DNA markers turned out to be an appropriate and substantial addition or even more a suitable alternative to isozyme markers for analysing genetic variation and testing provenance identity. Their advantages consist in a markedly wider variation as well as in the enlarged genome segments investigated.
Źródło:: Dendrobiology; 2009, 61 Supplement
1641-1307
Pojawia się w:: Dendrobiology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: A green light for plants
Autorzy:: Jasinski, M.
Powiązania:: https://bibliotekanauki.pl/articles/80884.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bioinformatics
climate change
demand
DNA marker
feed
food
food security
gene expression
global population
grain
green light
plant
plant biotechnology
plant genetics
plant science
worldwide consumption
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Description of DNA analysis techniques and their application in oat (Avena L.) genome research
Charakterystyka technik analiz DNA oraz ich wykorzystanie w badaniach owsa (Avena L.)
Autorzy:: Okon, S.
Kowalczyk, K.
Powiązania:: https://bibliotekanauki.pl/articles/26845.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: DNA analysis technique
application
oat
Avena
genome
molecular marker
crossbreeding efficiency
genetic map
hexaploid
diploid
plant species
crown rust
powdery mildew
plant resistance
DNA marker
polymerase chain reaction
Opis:: DNA markers are used not only to estimate genetic similarity and distance but also to select and identify desirable forms, to assess the adjustment of breeding material, to confirm crossbreeding efficiency, to determine seed purity, and to identify the genes which determine important functional traits. In the case of oat, DNA markers were used to construct and increase the density of genetic maps both in hexaploid and diploid species. The development of markers for some important traits provides a fast selection of genotypes containing dwarf genes as well as the resistance genes to crown rust and powdery mildew. Numerous analyses of genetic similarity between different species belonging to the genus Avena which are currently carried out may contribute to explaining the process of evolution within this genus and may also explain the development of particular species of oat.
Markery DNA znalazły zastosowanie nie tylko w ocenie podobieństwa lub dystansu genetycznego, ale również w selekcji i identyfikacji pożądanych form, ocenie wyrównania materiałów hodowlanych, potwierdzaniu skuteczności krzyżowań, ocenie czystości materiału siewnego czy też do identyfikacji genów, warunkujących ważne cechy użytkowe. U owsa posłużyły one między innymi do konstrukcji i zagęszczenia map genetycznych zarówno gatunków heksaploidalnych jak i diploidalnych. Opracowanie markerów dla niektórych cech użytkowych pozwala na szybką selekcję genotypów zawierających geny karłowatości, odporności na rdzę koronową czy mączniaka prawdziwego. Natomiast prowadzone liczne analizy podobieństwa genetycznego różnych gatunków z rodzaju Avena mogą przyczynić się do wyjaśnienia ewolucji w obrębie tego rodzaju jak również mogą wyjaśnić powstawanie poszczególnych gatunków owsa.
Źródło:: Acta Agrobotanica; 2012, 65, 1
0065-0951
2300-357X
Pojawia się w:: Acta Agrobotanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Pochodzenie drzewostanów świerkowych (Picea abies L. Karst.) z Puszczy Białowieskiej na tle regionu RDLP Białystok na podstawie analiz mitochondrialnego DNA
Origin of the Norway spruce (Picea abies L. Karst.) stands from Bialowieza Forest at the level of Bialystok Regional Directorate of the State Forests based on mitochondrial DNA analysis
Autorzy:: Nowakowska, J.A.
Łukaszewicz, J.
Borys, M.
Tereba, A.
Konecka, A.
Zawadzka, A.
Sułkowska, M.
Zajączkowski, P.
Powiązania:: https://bibliotekanauki.pl/articles/987121.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: lesnictwo
Puszcza Bialowieska
drzewostany swierkowe
drzewa lesne
swierk pospolity
Picea abies
markery genetyczne
DNA mitochondrialny
pochodzenie roslin
zmiennosc genetyczna
RDLP Bialystok
genetic diversity
mitochondrial dna marker
postglacial migration routes
białowieża forest
Opis:: Considered to be the most natural of all other Polish stands Picea abies in Białowieża Forest has suffered severely of bark beetle damages for decades. In order to find out some historical events in native Norway spruce stands diversity at regional level, we carried out the study on poly− morphism with DNA markers and performed Principal Coordinate Analysis (PCoA) on the basis of genetic distance matrix. Two ranges (i.e. Baltico−Nordic and Hercyno−Carpathian) of Norway spruce cover north−eastern and southern Poland, respectively. The spruceless zone separates the these two ranges in lowland Poland. From genetic point of view, the Baltico−Nordic P. abies populations mainly harbor mitochondrial haplotype ‘c', whereas the spruces from the Hercyno− −Carpathian range – haplotype ‘a'. Until recently, the Białowieża Forest has been considered to belong to the northern range of spruce occurrence in Europe. Seven populations from Biało− wieża Forest were studied (two of them from the Strict Reserve in Białowieski National Park) according to mitochondrial DNA diversity, and compared with genetic diversity found in 24 other populations located in Białystok Regional Directorate of the State Forests (north−eastern Poland). All studied spruce populations were more than 100 years old and were of natural origin. As result, the spruces from the Białowieża Forest harbored three mitochondrial haplotypes of the nad1 gene (‘c', ‘a' and ‘a1'), with the haplotype ‘a' being more frequently present (>51%) than the hap− lotype ‘c'. The spruce stands from Białowieża Forest have the highest gene diversity (h Nei=0.527) comparing to the other stands from the Białystok RDSF. The PCoA proved the particularity of the spruces from Białowieża Forest grouping them into one cluster of genetic similarity. Our data demonstrated that most of Norway spruces populations from Białystok RDSF harbor haplotype ‘c', which confirms their historical relationship with the Baltico−Nordic range of P. abies in Europe, while most spruces from Białowieża Forest have different historic origin, because they share high frequency of the southern haplotype ‘a'. It also turned out that Norway spruce pop− ulations from two different European ranges met in lowland Poland after the last glacial period, as proved by the presence of two mitochondrial haplotypes ‘a' and ‘c'. It can be assumed, that the spruceless zone was created by human activity in the past millennia. The results of all molecular analyses confirmed the unique character of spruces of Białowieża Forest, which is distinguished by the greater richness of the gene pool in comparison to the region of Białystok RDSF as well as good adaptation to local environmental conditions.
Źródło:: Sylwan; 2017, 161, 01; 40-51
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Markery DNA w hodowli jakosciowej rzepaku ozimego [Brassica napus L.] w aspekcie modyfikacji zawartosci kwasow tluszczowych
Autorzy:: Mikolajczyk, K
Bartkowiak-Broda, I.
Powiązania:: https://bibliotekanauki.pl/articles/834295.pdf
Data publikacji:: 2003
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: regulatory
rosliny oleiste
kwas laurynowy
hodowla jakosciowa
kwas palmitynowy
enzymy
kwas erukowy
kwas stearynowy
rzepak ozimy
kwas oleinowy
markery DNA
kwas mirystynowy
kwasy tluszczowe
Brassica napus
kwas linolenowy
regulatory enzyme
oil plant
lauric acid
qualitative breeding
palmitic acid
enzyme
erucic acid
stearic acid
winter rape
oleic acid
DNA marker
myristic acid
fatty acid
linolenic acid
Źródło:: Rośliny Oleiste - Oilseed Crops; 2003, 24, 1; 33-49
1233-8273
Pojawia się w:: Rośliny Oleiste - Oilseed Crops
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Pochodzenie świerka pospolitego (Picea abies (L.) Karst.) w Nadleśnictwie Kartuzy
Origin of Norway spruce (Picea abies (L.) Karst.) in the Kartuzy Forest District
Autorzy:: Lewandowski, A.
Szydlarski, M.
Litkowiec, M.
Powiązania:: https://bibliotekanauki.pl/articles/989990.pdf
Data publikacji:: 2014
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: lesnictwo
Nadlesnictwo Kartuzy
drzewa lesne
swierk pospolity
Picea abies
pochodzenie roslin
badania genetyczne
markery genetyczne
DNA mitochondrialny
origin
picea abies
mitochondrial marker
mitotype
Opis:: The origin of the Norway spruce in the Kartuzy Forest District was verified using the maternally inherited mitochondrial DNA marker mt15−D02 that shows distinct geographical distribution in Europe. We analyzed 462 trees from 26 populations. The majority of trees were of Alpine origin and they were present in all populations at a frequency of 6−90% (mean 59%). About 30% of trees were of the Carpathian origin and the least abundant (11%) were trees of north−eastern origin. The trees carrying north−eastern type of the mitochondial DNA marker were found only in 13 populations, but at a very different frequency (from 5 to 88%). The possibility of natural origin of Norway spruce in the area of Kartuzy Forest District was shortly discussed.
Źródło:: Sylwan; 2014, 158, 07; 509-515
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 16.

Tytuł:: Assessment of 8-oxo-7,8-dihydro-2′-deoxyguanosine as a marker of oxidative DNA damage in gasoline filling station attendants
Autorzy:: Beerappa, Ravichandran
Venugopal, Dhananjayan
Sen, Somnath
Ambikapathy, Mala
Rao, Rajmohan H.
Powiązania:: https://bibliotekanauki.pl/articles/2179055.pdf
Data publikacji:: 2013-10-01
Wydawca:: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:: DNA damage
ELISA
petrol filling attendants
urinary 8-oxo-7
8-dihydro-2’-deoxyguanosine
Opis:: Objectives: The urinary excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) was used as a biomarker of oxidative DNA damage. The urinary 8-oxodG levels in petrol filling station attendants (exposed) at various petrol bunks were estimated as well as in the unexposed (cashier) population. Materials and Methods: A total of 100 workers (79 petrol fillers and 21 cashiers) aged from 20 to 41 years participated in the study. An informed consent was taken from each participant. Information on personal habits and health was obtained through a questionnaire. After shifts, urine samples were collected analyzed for 8-oxodG using enzyme-linked immunosorbent assay (ELISA). Results: Fifty-three percent of workers were in the 21-30 years age group. The maximum level of 8-oxodG was observed in the age group ≥ 41 years and the minimum in the age group of 31-40 years. The maximum level of 8-oxodG was observed among those workers who had ≥ 21 years of experience. The concentrations of 8-oxodG were significantly higher in petrol fillers than those in cashiers (p < 0.05). Conclusions: Despite the conflicting results obtained in our study it was shown that 8-oxodG is related to chemical exposure. Further research is needed embracing a bigger number of participants to highlight the correlations between the exposure and the effects.
Źródło:: International Journal of Occupational Medicine and Environmental Health; 2013, 26, 5; 780-789
1232-1087
1896-494X
Pojawia się w:: International Journal of Occupational Medicine and Environmental Health
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 17.

Tytuł:: Postglacjalna migracja jodły pospolitej (Abies alba Mill.) do Polski - analiza na podstawie polimorfizmu mitochondrialnego DNA
Postglacial migration of silver fir (Abies alba Mill.) to Poland - analysis on the basis of mitochondrial DNA polymorphism
Autorzy:: Pawlaczyk, E. M.
Kroplewska, I.
Bobowicz, M. A.
Powiązania:: https://bibliotekanauki.pl/articles/991708.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: drzewa lesne
migracje roslin
okres polodowcowy
Polska
jodla pospolita
Picea abies
populacje roslin
badania molekularne
DNA mitochondrialny
polimorfizm DNA
silver fir (abies alba mill.)
refugium
postglacial migration
mitochondrial marker
Opis:: Mitochondrial DNA marker was applied to 10 populations of silver fir from Poland and one from Belarusia. These populations were located within and beyond the natural range of silver fir. The marker exhibited two highly conserved alleles (first – 230 bp and second – 150 bp) based on an insertion−deletion of 80 bp in the fourth intron of the mitochondrial nad5 gene. The geographical distribution of the maternally inherited mitochondrial variation is known to support the existence of at least two refugia with two recolonizing maternal lineages remaining largely separated throughout the range. Our results provide that in all studied populations the first allele was discovered. Therefore we postulate that the silver fir migrate to Poland from the refugium in western Europe (probably from central Italy).
Źródło:: Sylwan; 2013, 157, 06; 458-463
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 18.

Tytuł:: Molekularna identyfikacja gatunków z rodzaju Abies na podstawie polimorfizmu DNA mitochondrialnego
Molecular identification of species from Abies genus based on the mitochondrial DNA polymorphism
Autorzy:: Pawlaczyk, E.M.
Staniak, J.
Maliński, T.
Bobowicz, M.A.
Powiązania:: https://bibliotekanauki.pl/articles/989708.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: lesnictwo
drzewa lesne
genetyka roslin
jodla
Abies
gatunki roslin
identyfikacja
haplotypy
DNA mitochondrialny
polimorfizm DNA
abies species
haplotype
capillary electrophoresis
mitochondrial marker
Opis:: The plant material was collected on 34 individuals growing in the Dendrological Garden of Poznań University of Life Sciences (52°25'32,95" N 16°53'39,83" E) and Botanical Garden of Adam Mickiewicz University in Poznań (52°25'11,70" N 16°52'55,07" E). The species for this study originated from Europe, Asia Minor, central and eastern Asia and North America and included: Abies alba, Abies cephalonica, Abies cilicica, Abies equi−trojani, Abies sibirica, Abies koreana, Abies pinsapo, Abies ×insignis, Abies bornmulleriana, Abies homolepsis, Abies holophylla, Abies grandis, Abies concolor, Abies concolor var. violacea, Abies concolor var. lowiana, Abies nordmanniana, Abies ×arnoldiana, Abies nephrolepis and Abies balsamea. The aim of this study was to define the species haplotypes (the length of allele) on the basis of nad5−4 mitochondrial DNA marker detected by capillary electrophoresis. This marker has been suggested as an easy−to−use tool to distinguish species of the Abies genus and it could be species−specific. Seven different haplotypes were identified. The first one appears in the species from Europe, Asia and North America. The second one was detected in firs from Europe and Asia Minor. A. cephalonica and A. sibirica were identified by the third haplotype, which occurs also in A. alba from the Balkan region. The fourth haplotype is characteristic for species from Asia and North America. The fifth and sixth haplotypes were identified in A. pinsapo and A. numidica. The seventh haplotype was detected only in A. holophylla. Applied marker is a very useful for verification of fir species especially allopatric species, less for parapatric ones. This marker is more helpful to exclude the species than to precisely identify them.
Źródło:: Sylwan; 2015, 159, 08; 675-683
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 19.

Tytuł:: Zastosowanie metody AFLP do analizy DNA rzepaku ozimego.
Autorzy:: Matuszczak, M
Powiązania:: https://bibliotekanauki.pl/articles/833457.pdf
Data publikacji:: 2002
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: rosliny oleiste
genetyka roslin
analiza DNA
markery molekularne
rzepak ozimy
oil plant
plant genetics
DNA analysis
molecular marker
winter rape
Źródło:: Rośliny Oleiste - Oilseed Crops; 2002, 23, 2; 255-265
1233-8273
Pojawia się w:: Rośliny Oleiste - Oilseed Crops
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 20.

Tytuł:: DNA fingerprints generated by R.18.1 DNA probe in two stocks of chickens: Green Legged Patridgenous [GLP] and Rhode Island Red [RIR]
Autorzy:: Rosochacki, S J
Hillel, J
Jaszczak, K
Zawadzka, M
Powiązania:: https://bibliotekanauki.pl/articles/2046824.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: multi-locus DNA probe
hybridization
animal breeding
poultry
molecular marker
DNA
chicken
Opis:: Hybridization of a multi-locus DNA probe, R 18.1, to genomic DNA from poultry showed a highly polymorphic fingerprint pattern. The detected DNA fingerprints are individually specific and differ between Green Legged Patridgenous (GLP) and Rhode Island Red (RIR) stock of chickens. The average numbers of detected bands in RIR were 18.68 and in GLP - 15.33, but the average band sharing levels were 0.619 and 0.431, respectively. The level of polymorphism may be connected possibly with a higher level of inbreeding in the examined stock of chicken.
Źródło:: Journal of Applied Genetics; 1997, 38, 2; 173-178
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 21.

Tytuł:: Integrated statistical and rule-mining techniques for dna methylation and gene expression data analysis
Autorzy:: Mallik, S.
Mukhopadhyay, A.
Maulik, U.
Powiązania:: https://bibliotekanauki.pl/articles/1396742.pdf
Data publikacji:: 2013
Wydawca:: Społeczna Akademia Nauk w Łodzi. Polskie Towarzystwo Sieci Neuronowych
Tematy:: statistical analysis
gene marker
methylation
genetic algorithm
DNA
Opis:: For determination of the relationships among significant gene markers, statistical analysis and association rule mining are considered as very useful protocols. The first protocol identifies the significant differentially expressed/methylated gene markers, whereas the second one produces the interesting relationships among them across different types of samples or conditions. In this article, statistical tests and association rule mining based approaches have been used on gene expression and DNA methylation datasets for the prediction of different classes of samples (viz., Uterine Leiomyoma/class-formersmoker and uterine myometrium/class-neversmoker). A novel rule-based classifier is proposed for this purpose. Depending on sixteen different rule-interestingness measures, we have utilized a Genetic Algorithm based rank aggregation technique on the association rules which are generated from the training set of data by Apriori association rule mining algorithm. After determining the ranks of the rules, we have conducted a majority voting technique on each test point to estimate its class-label through weighted-sum method. We have run this classifier on the combined dataset using 4-fold cross-validations, and thereafter a comparative performance analysis has been made with other popular rulebased classifiers. Finally, the status of some important gene markers has been identified through the frequency analysis in the evolved rules for the two class-labels individually to formulate the interesting associations among them.
Źródło:: Journal of Artificial Intelligence and Soft Computing Research; 2013, 3, 2; 101-115
2083-2567
2449-6499
Pojawia się w:: Journal of Artificial Intelligence and Soft Computing Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 22.

Tytuł:: Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland
Autorzy:: Nowakowska, J A
Powiązania:: https://bibliotekanauki.pl/articles/40976.pdf
Data publikacji:: 2009
Wydawca:: Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:: international conference
Europe
forest ecosystem
plant breeding
tree
Norway spruce
Picea abies
Polska
mitochondrial DNA
nuclear DNA
plant population
microsatellite marker
post-glacial migration
Opis:: The natural stands of Norway spruce in Poland are split between the southern and the northeastern parts of the country. Two so-called "spruceless" zones separate the northern spruce locations from those in the south, one "spruceless" zone is situated in Central Poland, and the other one in the Beskid Mts. Mitochondrial (STS) and nuclear (SSR) markers were used to perform the genetic identification of Norway spruce. Four different variants of haplotypes, "a", "b", "c" and "d", were found to occur in the nad1 locus of STS markers. Populations from the northern range of Picea abies distribution in Poland harboured exclusively haplotypes "c" and "d", except for the Białowieża population which had haplotypes "a" and "c". Populations from the "spruceless" zones contained four types of haplotypes whilst those from southern Poland were mostly composed of haplotype "a". High mean gene diversity was observed for both STS and SSR markers (HT = 0.529, and HT = 0.851, respectively). The total genetic differentiation of Norway spruce populations was very low (FST= 0.088). Two main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers. The distribution of the genotypes was scattered and did not show any connection with the spatial distribution of P. abies in Poland. Only the mtDNA markers were able to differentiate the northern populations of Norway spruce from the southern ones, proving the historical separation between the Baltico-Nordic and the Hercyno-Carpathian ranges of P. abies in Poland. By contrast, the microsatellite data suggested an overlap between the genotypes due to the human manipulation of Norway spruce stands in the past.
Źródło:: Dendrobiology; 2009, 61 Supplement
1641-1307
Pojawia się w:: Dendrobiology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 23.

Tytuł:: Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker
Autorzy:: Mullainathan, L.
Sridevi, A.
Umavathi, S.
Sanjai Gandhi, E.
Powiązania:: https://bibliotekanauki.pl/articles/11592.pdf
Data publikacji:: 2014
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: genetic variation
mutant
chilli
Capsicum annuum
random amplified polymorphic DNA analysis
RAPD marker
spice
Opis:: The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
Źródło:: International Letters of Natural Sciences; 2014, 06
2300-9675
Pojawia się w:: International Letters of Natural Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 24.

Tytuł:: Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam
Autorzy:: Ho, V.T.
Ngo, Q.N.
Powiązania:: https://bibliotekanauki.pl/articles/79939.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Dimocarpus longan
longan
fruit
Vietnam
genetic conservation
morphological characteristics
chemical characteristics
SCAR marker
random amplified polymorphic DNA analysis
molecular marker
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 25.

Tytuł:: Use of random amplified polymorphic DNA [RAPD] assay for differentiation among isolates of Stagonospora spp. and Septoria tritici
Autorzy:: Czembor, P C
Arseniuk, E
Powiązania:: https://bibliotekanauki.pl/articles/2047271.pdf
Data publikacji:: 1996
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Stagonospora
Stagonospora avenae
Septoria tritici
random amplified polymorphic DNA
pathogen
polymerase chain reaction
DNA
molecular marker
fungal isolate
Stagonospora nodorum
genetic variation
Opis:: The genetic similarity of three species: Septoria tritici, Stagonospora nodorum and Stagonospora avenae f. sp. triticea - important pathogens in many cereal production areas worldwide was assessed by random amplified polymorphic DNA (RAPD) assay. In preliminary research DNA of 14, 9, and 7 monopyenidios- pore isolates of S. nodorum, S. tritici, and S. a. tritícea, respectively, were amplified by PCR with four primers. Afterwards the research was focused on three mono- pyenidiospore isolates from each species studied. The isolates of each species selected for the study varied in pathogenicity and were diverse geographically. PCR with the set of 14 selected primers resulted in 99 different bands, ranged from 180 to 2500 base pairs in length. Most primers in PCR (especially RAD11, RAD31, RAD32, RAD33) revealed uniform bands for isolates of S. a. tritícea, that allow to identify this species among the others. The cluster analysis using Unweighed Pair-Group Method with Averaging (UPGMA) revealed interspecies disagreement among the isolates ranging from 32 to 53%. The intraspecies disagreement ranges were 17-20%, 38-43%, 42-44% for S. avenae f. sp. triticea, S. nodorum and S. tritici, respectively. Cluster analysis classified isolates into three homogeneous clusters. Each cluster grouped isolates of one species according to their current taxonomie ranks based on spore size, colony morphology and host ranges. In addition, two of the clusters represented by isolates of S. nodorum and S. a. tritícea were distinctly separated at a lower linkage distance from the third one comprising isolates of S. tritici. A slight inconsistency found in grouping some isolates indicates that such groupings should be done with caution. The present study indicates that the PCR- RAPD assay is of a potential use in taxonomy of Stagonospora spp. and Septoria tritici as well as in molecular identification of casual disease agents.
Źródło:: Journal of Applied Genetics; 1996, 37, 3; 239-251
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 26.

Tytuł:: A new RAPD marker identifying restorer lines for CMS ogura system
Nowy marker typu RAPD identyfikujacy linie restorery dla systemu CMS ogura
Autorzy:: Furguth, A
Bartkowiak-Broda, I.
Powiązania:: https://bibliotekanauki.pl/articles/834103.pdf
Data publikacji:: 2005
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: oilseed rape
CMS-ogura system
restorer line
hybrid
winter oilseed rape
Brassica napus
random amplified polymorphic DNA
Rfo restorer gene
molecular marker
Źródło:: Rośliny Oleiste - Oilseed Crops; 2005, 26, 2; 595-602
1233-8273
Pojawia się w:: Rośliny Oleiste - Oilseed Crops
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 27.

Tytuł:: Zastosowanie markerów DNA w selekcji marchwi z cechą cytoplazmatycznej męskiej sterylności typu płatkowego
The use of DNA markers in the selection of carrot lines with cytoplasmic male sterility trait
Autorzy:: Szczechura, W.
Nowakowska, M.
Nowak, K.
Kamiński, P.
Nowak, R.
Kozik, E.U.
Powiązania:: https://bibliotekanauki.pl/articles/2119577.pdf
Data publikacji:: 2019
Wydawca:: Instytut Ogrodnictwa
Tematy:: CMS
carrot
DNA markers
marker-assisted selection (MAS)
Opis:: Cytoplasmic male sterility (CMS) is a maternally inherited trait in which a plant is unable to produce functional pollen. It occurs in many plants, including carrots. The expression of this trait depends on environmental conditions, especially temperature – therefore, DNA markers become an excellent tool for selecting carrot genotypes with this feature. In this study, we used seventeen DNA markers available in the literature, linked with various mitochondrial DNA sequences that are associated with the CMS trait. Their amplification was tested on male sterile and male fertile plants. Only five markers – A321/392, I214/401/435, K751, N795, O790 – correctly identified the tested genotypes. They can be used as selection tools in carrot breeding programs.
Źródło:: Zeszyty Naukowe Instytutu Ogrodnictwa; 2019, 27; 53-62
2300-5882
2391-8969
Pojawia się w:: Zeszyty Naukowe Instytutu Ogrodnictwa
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 28.

Tytuł:: Powdery mildew resistance genes in wheat: verification of STS markers
Autorzy:: Stepien, L
Chen, Y.
Chelkowski, J.
Kowalczyk, K.
Powiązania:: https://bibliotekanauki.pl/articles/2048311.pdf
Data publikacji:: 2001
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Aegilops speltoides
randomly amplified polymorphic DNA analysis
powdery mildew
wheat
wheat cultivar
Sequence Tagged Site marker
resistance gene
Pm gene
Źródło:: Journal of Applied Genetics; 2001, 42, 4; 413-423
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 29.

Tytuł:: Transformation of wild Solanum species resistant to late blight by using reporter gene gfp and msh2 genes
Autorzy:: Rakosy-Tican, Lenuta
Aurori, Adriana
Aurori, Cristian M.
Ispas, Gabriela
Famelaer, Ivan
Powiązania:: https://bibliotekanauki.pl/articles/2198986.pdf
Data publikacji:: 2004-12-20
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: Agrobacterium tumefaciens mediated transformation
DNA mismatch repair
gfp
nptII marker gene
Opis:: Green fluorescent protein (gfp) reporter gene and nptII marker gene were used to optimize Agrobacterium tumefaciens (agro) mediated transformation of wild Solanum genotypes resistant to late blight. Different genotypes of Solanum bulbocastanum, S. chacoense, S. microdontum and S. verrucosum were assessed for their regeneration ability on MS based media and for agro-mediated transformation. As the first step reporter genes were used to optimize transformation protocol for each species and then the transfer of genes involved in mismatch repair of DNA were attempted in Solanum chacoense. For transformation, either leaf or stem fragments were used. It was shown that gfp is a valuable and elegant tool for monitoring the efficiency of transformation or the occurrence of chimera in all genotypes. Transformation efficiency was dependent on a plant genotype. A number of genotypes have been successfully transformed and they expressed constitutively the bright green fluorescence of gfp without any side effects. The most recalcitrant species proved to be S. microdontum, which did not regenerate plants although different media and phytohormones had been used. The best protocol for S. chacoense transformation was also found to work in the transfer of msh2 genes. Msh2 isolated from Arabidopsis was used and transferred either as mutated (Apa) or antisense (As) gene. The integration of msh2-mutated gene into S. chacoense genome was demonstrated by PCR amplification and confirmed by RT-PCR for some of the putative transgenic clones. The implications of mismatch repair in homologous recombination and its importance for potato improvement are discussed.
Źródło:: Plant Breeding and Seed Science; 2004, 50; 119-127
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 30.

Tytuł:: Somatic embryogenesis and genetic uniformity of regenerated cassava plants from low-temperature preserved secondary somatic cotyledons
Autorzy:: Opabode, J.T.
Ajibola, O.V.
Oyelakin, O.O.
Akinyemiju, O.A.
Powiązania:: https://bibliotekanauki.pl/articles/79828.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cassava
Manihot esculenta
somatic embryogenesis
plant regeneration
cotyledon
organogenesis
DNA extraction
RAPD marker
dehydration
low temperature
regeneration
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 31.

Tytuł:: In vitro and molecular characterization using ISSR markers of Glycyrrhiza glabra L.
Autorzy:: El-Hameid, A.A.
El-Kheir, Z.A.
Abdel-Hady, M.
Helmy, W.
Powiązania:: https://bibliotekanauki.pl/articles/80908.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: licorice
Glycyrrhiza glabra
callus induction
genomic DNA
ISSR marker
molecular characteristics
polymerase chain reaction
kinetin
Murashige medium
Skoog's medium
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 32.

Tytuł:: A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia
Autorzy:: Sawicki, J.
Szczecinska, M.
Powiązania:: https://bibliotekanauki.pl/articles/57748.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: Acutifolia
random amplified polymorphic DNA
Sphagnum
genetic similarity
molecular identification
molecular marker
polymerase chain reaction
genetic relationship
species identification
peat moss
chloroplast
nuclear genome
Opis:: RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.
Źródło:: Acta Societatis Botanicorum Poloniae; 2011, 80, 3
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 33.

Tytuł:: Cryptic rearrangements of chromosome 12 in testicular germ cell tumors with or without the specific i[12p] marker
Autorzy:: Grygalewicz, B
Pienkowska-Grela, B.
Woroniecka, R.
Powiązania:: https://bibliotekanauki.pl/articles/2043149.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: aberration
in situ
isochromosome
cytogenetic analysis
microdissection
testicular germ cell tumour
karyotype
cell culture
nonseminoma
fluorescence
hybridization
seminoma
chromosome 12
DNA
Źródło:: Journal of Applied Genetics; 2000, 41, 2; 123-131
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 34.

Tytuł:: Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers
Analiza zróżnicowania genetycznego wśród genotypów Arnica montana L. za pomocą markerów RAPD
Autorzy:: Okoń, S.
Paczos-Grzęda, E.
Łoboda, M.
Sugier, D.
Powiązania:: https://bibliotekanauki.pl/articles/11542962.pdf
Data publikacji:: 2014
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: DNA polymorphism
identification
genetic diversity
Arnica montana
genotype
RAPD marker
medicinal plant
molecular analysis
Opis:: Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.
Arnica montana L. jest jedną z najcenniejszych roślin zielarskich wykorzystywanych w medycynie, farmacji i przemyśle kosmetycznym. W dostępnej literaturze liczba doniesień związanych z analizą molekularną arniki jest znikoma, dlatego też celem prezentowanych badań była optymalizacja procesu izolacji DNA ze świeżych liści oraz identyfikacja zróżnicowania genetycznego oparta na markerach RAPD. W prezentowanej pracy w celu uzyskania czystego DNA do izolacji wykorzystano zestaw DNA Plant & Fungi DNA Purification Kit (Euro) oraz oczyszczanie za pomocą długiej elektroforezy w żelu agarozowym. Spośród testowanych 40 starterów RPAD do analiz wybrano 12 generujących stabilne i polimorficzne wzory prążków. Wyselekcjonowane startery amplifikowały 120 fragmentów, spośród których 111 (92,5%) było polimorficznych. Wykorzystujac markery RAPD utworzono matryce podobieństwa genetycznego. średnia wartość podobieństwa analizowanych genotypów wynosiła 0.886. Najwyższy współczynnik podobieństwa genetycznego oszacowano pomiędzy genotypami GA17 i GA18, które ulokowały się blisko siebie na uzyskanym dendrogramie.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 63-71
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Tytuł:: A fast and effective protocol for obtaining genetically diverse stevia (Stevia rebaudiana Bertoni) regenerants through indirect organogenesis
Szybki i efektywny protokół otrzymywania zróżnicowanych genetycznie regenerantów stewii (Stevia rebaudiana Bertoni) drogą pośredniej organogenezy
Autorzy:: Dyduch-Siemińska, M.
Powiązania:: https://bibliotekanauki.pl/articles/13925501.pdf
Data publikacji:: 2021
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: stevia
Stevia rebaudiana
micropropagation
molecular marker
random amplified polymorphic DNA
shoot regeneration
somaclonal variation
Źródło:: Agronomy Science; 2021, 76, 4; 47-62
2544-4476
2544-798X
Pojawia się w:: Agronomy Science
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Śladami badań Władysława Jedlińskiego – określenie pochodzenia świerka na terenie Nadleśnictwa Skrwilno
Following the research of Wladyslaw Jedlinski – determination of the origin of spruce in the Skrwilno Forest District
Autorzy:: Lewandowski, A.
Litkowiec, M.
Fischer, A.
Powiązania:: https://bibliotekanauki.pl/articles/1008413.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: lesnictwo
Nadlesnictwo Skrwilno
drzewa lesne
swierk pospolity
Picea abies
pochodzenie roslin
markery genetyczne
DNA mitochondrialny
origin
picea abies
mitochondrial marker
Opis:: Origin of Norway spruce in the territory of Skrwilno Forest District was studied with the use of mitochondrial mt−D02 region inherited through maternal line. The former suppositions of Jedliński, who claimed that the origin of spruce in this territory was natural, have been confirmed. Concurrently, it has been established that in the majority of tree stands subject to our study there occurs spruce of Carpathian origin. The obtained results together with the palinological data indicate that the northern border of spruce range in Poland runs right through the territory of Skrwilno Forest District and not about 100 km to the south from this place as has been claimed so far.
Źródło:: Sylwan; 2012, 156, 09; 703-709
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 37.

Tytuł:: Molecular studies in osteogenesis imperfecta [OI] II. Evaluation of intragenic polymorphic sites in COL1A1 and COL1A2 loci
Autorzy:: Kostyk, E
Sucharski, P.
Pietrzyk, J.J.
Kruczek, A.
Powiązania:: https://bibliotekanauki.pl/articles/2044212.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: intragenic polymorphic site
polymorphism
haplotype
DNA isolation
COL1A1 gene
electrophoresis
collagen
COL1A2 gene
molecular marker
osteogenesis imperfecta
Opis:: The goal of the study was to evaluate intragenic polymorphic sites in COL1A1 and COL1A2 loci. For COL1A1 the following intragenic markers were used: PCR-RFLP (COL1A1), G/A polymorphism in exon 45 of COL1A1 and C/T polymorphism in +88 position of COL1A1 non-translatable 3’ end. For COL1A2 PCR-VNTR was analyzed. 17 families were examined (6 of the "simplex" type and 11 of the "multiple" type). In 8 out of 11 "multiplex" families the segregation of the markers revealed correlation with OI, whereas the other 3 were non-informative. The method was not useful in "simplex" families.
Źródło:: Journal of Applied Genetics; 1998, 39, 4; 349-365
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Charakterystyka samosiewów rzepaku ozimego (Brassica napus L.) za pomocą markerów RAPD
Characteristics of winter oilseed rape (Brassica napus L.) volunteers with the use of RAPD markers
Autorzy:: Bocianowski, Jan
Lieesch, Alina
Bartkowiak-Broda, Iwona
Popławska, Wiesława
Powiązania:: https://bibliotekanauki.pl/articles/41491656.pdf
Data publikacji:: 2008
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: markery molekularne RAPD
polimorfizm DNA
rzepak ozimy (Brassica napus L.)
samosiewy
molecular marker RAPD
polymorphism DNA
winter oilseed rape (Brassica napus L.)
volunteers
Opis:: Celem pracy była ocena podobieństwa genetycznego (GS) pomiędzy samosiewami oraz odmianami rzepaku i rzepiku, a także określenie związku markerów molekularnych z cechami fenotypowymi i genotypem. Badania obejmowały potomstwo 31 samosiewów pobranych z plantacji rzepaku ozimego podwójnie ulepszonego w sezonie 2005/2006 w trzech województwach Polski północnej. Jako wzorce wybrano odmiany rzepaku uprawiane na plantacjach, z których pobrano samosiewy: Californium, Castille, Lisek i Rasmus oraz odmianę rzepiku ozimego (B. campestris) Ludowy. Charakterystykę samosiewów rzepaku wykonano za pomocą 431 markerów molekularnych typu RAPD. Dendrogram utworzony w oparciu o miarę GS Nei i Li (1979) rozdzielił badane genotypy na dwie zasadnicze grupy: samosiewy o morfotypie rzepaku i odmiany wzorcowe rzepaku oraz grupę obejmującą rośliny o morfotypie rzepiku i odmianę rzepiku Ludowy. Stwierdzono także istotny związek pomiędzy grupami markerów RAPD a cechami fenotypowymi i poziomem ploidalności. Otrzymano 21 markerów RAPD występujących wyłącznie w roślinach rzepiko¬podobnych o podwyższonej zawartości kwasu erukowego oraz 59 markerów charakterystycznych dla roślin w typie rzepaku o niskiej zawartości kwasu erukowego.
This study aimed to estimate genetic similarity (GS) and relationships between molecular markers and phenotypic traits and between molecular markers and genotype. The investigations included progenies of 31 volunteers collected from winter oilseed rape fields in the growing season 2005/2006. The plantations were selected in three Voivodships in northern Poland. Winter oilseed rape cultivars Californium, Castille, Lisek, Rasmus, cultivated in the fields of origin of volunteers, and winter turnip rape (B. campestris) Ludowy were chosen as standards. The volunteers of winter oilseed rape have been investigated through 431 RAPD markers. Dendrogram based on Nei and Li (1979) coefficient grouped genotypes in two clusters. The first one was represented by oilseed rape-like plants and standard oilseed rape cultivars; the second group consisted of turnip rape-like plants and turnip rape cultivar Ludowy. Significant associations between molecular markers, phenotypic traits and genotypes were found. Twenty-one of the molecular markers were specific for the turnip rape-like plants with high erucic acid content, and 59 markers were specific for oilseed rape-like plants with low erucic acid content.
Źródło:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2008, 249; 183-192
0373-7837
2657-8913
Pojawia się w:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:: Biblioteka Nauki