Wszystkie pola: DNA gel electrophoresis - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Study of Changes in Genetic Diversities and Hepatic Histological Structures of Two Ichthyofauna as a Consequence of Water Pollution in Two Different Areas of West Bengal
Autorzy:: Lahiri, Annesha
Das, Sohini
Datta, Rahul Kumar
Powiązania:: https://bibliotekanauki.pl/articles/1193627.pdf
Data publikacji:: 2017
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: DNA gel electrophoresis
Labeo bata
Labeo rohita
Water pollution
fish liver
histopathology
ichthyofauna
physicochemical parameters
Opis:: Water pollution affects biodiversity harshly. An attempt was made to find out the effect of aquatic pollution on the tissues of the fish, taking two different types of carps from two different areas of West Bengal (Barrackpore in North 24 Parganas and Raiganj in North Dinajpur). The carps used were Labeo rohita (major carp) and Labeo bata (minor carp) and were sampled from two different ponds within 1 km of each locality. The physicochemical parameters of the respective ponds were estimated from which the fishes were collected and comparisons were made. Liver tissues were taken from the fishes for histological and molecular analysis. From histological studies, it was found that there were some irregularities in the liver tissue structures which might be due to water pollution. DNA isolation from fish liver followed by gel electrophoresis was done to check whether water pollution affect the integrity of the extracted genomic DNA of the fish samples.
Źródło:: World Scientific News; 2017, 71; 132-138
2392-2192
Pojawia się w:: World Scientific News
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: DNA double-strand break rejoining in radioadapted human lymphocytes: evaluation by neutral comet assay and pulse-field gel electrophoresis
Autorzy:: Wojewódzka, M.
Buraczewska, I.
Szumiel, I.
Grądzka, I.
Powiązania:: https://bibliotekanauki.pl/articles/146153.pdf
Data publikacji:: 2006
Wydawca:: Instytut Chemii i Techniki Jądrowej
Tematy:: human lymphocytes
radioadaptation
DNA double-strand break rejoining
neutral comet assay
pulsefield gel electrophoresis
Opis:: Adaptive response (AR), an enhanced resistance to a high dose of ionising radiation acquired after pretreatment with a very low dose, was estimated in normal human lymphocytes. The question posed was whether the extent of radioadaptation, assessed by micronucleus test, would be related to the rate of DNA double-strand break (DSB) rejoining. Phytohemagglutinin-stimulated G1-lymphocytes from 5 healthy male volunteers were pre-treated (or not) with an adaptive (5 cGy) dose of X-rays, followed by a higher (5 or 10 Gy) challenge dose after 20-22 h. DSB rejoining after the challenge dose was monitored with the use of two methods: neutral comet assay, modified to reduce the contribution of single-strand breaks (SSBs) and thermolabile sites, and pulse-field gel electrophoresis (PFGE), specific for DSBs. At the level of micronuclei, an AR was observed in lymphocytes of 3 of 5 donors. Up to 60 min, comet assay showed no statistically significant differences in DNA break rejoining between adapted and non-adapted lymphocytes, independently of AR appearance. PFGE gave similar results, although in three donors it revealed secondary increases in DSBs levels at 30 min and/or 60 min post-irradiation in the adapted vs. the non-adapted samples. Failure to demonstrate changes in DSBs rejoining rate in the adapted lymphocytes could be due to diversity of AR intensity/timing at the level of DNA repair in not fully homogenous cell populations. Also, “rare” DNA cuts characteristic of early apoptosis/necrosis could overlap the process of DNA break rejoining.
Źródło:: Nukleonika; 2006, 51, 4; 185-191
0029-5922
1508-5791
Pojawia się w:: Nukleonika
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Electrophoretic seed albumin patterns in the Vicia sativa L. aggregate
Autorzy:: Przybylska, J
Zimniak-Przybylska, Z.
Krajewski, P.
Powiązania:: https://bibliotekanauki.pl/articles/2043428.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: polyacrylamide gel electrophoresis
genetic resource
chromosome number
Vicia sativa
variation
DNA polymorphism
vetch
seed albumin
electrophoresis
karyotype
isoenzyme variation
taxonomy
Źródło:: Journal of Applied Genetics; 2000, 41, 3; 139-149
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Fractionation of DNA isolated from lymphocytes of patients under therapy with the antiblastic drug cis-diamminedichloroplatinum(II) using pulsed field gel electrophoresis
Autorzy:: Jaruga, Paweł
Zastawny, Tomasz
Tujakowski, Jerzy
Oliński, Ryszard
Powiązania:: https://bibliotekanauki.pl/articles/1045507.pdf
Data publikacji:: 1993
Wydawca:: Polskie Towarzystwo Biochemiczne
Źródło:: Acta Biochimica Polonica; 1993, 40, 1; 60-62
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Comparison of the effects of bleomycin and ionizing radiation in two sublines of murine lymphoma L5178Y
Autorzy:: Kruszewski, M.
Zaim, J.
Grądzka, I.
Szumiel, I.
Powiązania:: https://bibliotekanauki.pl/articles/147561.pdf
Data publikacji:: 2001
Wydawca:: Instytut Chemii i Techniki Jądrowej
Tematy:: bleomycin
comet assay
DNA damage
gamma radiation
single cell gel electrophoresis
Opis:: We compared the effects of bleomycin (BLM) and ionizing radiation on two sublines of murine lymphoma L5178Y (LY): LY-R, radiation resistant and LY-S, radiation sensitive. This radiosensitivity difference is related to the ability to rejoin DNA double strand breaks. LY-S cells were about two times more sensitive to BLM than LY-R, similarly as in the case of sensitivity to X rays. Since there was no difference in the P-glycoprotein-related drug transport system between the sublines, it could be expected that the enhanced sensitivity of LY-S cells to BLM was caused by the DNA repair defect. Growth disturbances in BLM treated cell populations were proportional to the lethal effect and their duration was observed until elimination of dead cells (3-6 days after 50 ěM BLM, 1 h at 37oC). There was no slow growth phase accompanied by normal viability, as previously described for X-irradiated LY-S cells. Initial DNA damage, estimated with the single cell gel electrophoresis method was linearly related to BLM dose in LY-S cells; in LY-R cells - in the low dose range (up to 10 ěM) - there was more damage than in LY-S cells, however, at higher doses the dose - effect curves became identical. The doseeffect relationship for ă rays was linear and identical in both cell sublines. DNA damage distribution in BLM treated cells was much less uniform as compared to that in irradiated cells and indicated the presence of cells with severely damaged DNA, a feature typical for BLM action in vitro.
Źródło:: Nukleonika; 2001, 46, 3; 81-86
0029-5922
1508-5791
Pojawia się w:: Nukleonika
Dostawca treści:: Biblioteka Nauki

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