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Wyświetlanie 1-15 z 43
Tytuł:
Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase
Autorzy:
Li, Shu
Chen, Ming
Li, Gang
Yan, Yong
Yu, Hai
Zhan, Yu
Peng, Zi
Wang, Jin
Lin, Min
Powiązania:
https://bibliotekanauki.pl/articles/1040841.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Zymomonas mobilis
His296
levansucrase
levan
transfructosylation activity
hydrolysis activity
Opis:
His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge and length. The substitutions of His296 with Arg or Trp retained partial hydrolysis and transfructosylation activities. The positively charged Lys substitution resulted in a 2.5-fold increase of sucrose hydrolysis. Substitutions with short (Cys or Ser), negatively charged (Glu) or polar (Tyr) amino acids virtually abolished both the activities. Analysis of transfructosylation products indicated that the mutants synthesized different oligosaccharides, suggesting that amino acid substitutions of His296 strongly affected both the enzyme activity and transfructosylation products.
Źródło:
Acta Biochimica Polonica; 2008, 55, 1; 201-206
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toluene Mono-nitration in a Semi-batch Reactor
Autorzy:
Chen, L. P.
Chen, W. P.
Liu, Y.
Peng, J. H.
Liu, R. H.
Powiązania:
https://bibliotekanauki.pl/articles/358853.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
toluene mono-nitration
apparent activation energy
semi-batch reaction
thermal hazard
Opis:
Toluene mono-nitration in a semi-batch reactor was investigated with reaction calorimeter (RC1). The mixed acid HNO3/H2SO4/H2O (wt%) was used in proportion 13/66/21, which is similar to industrial parameters. The exothermic rates at different reaction temperatures were compared, and then the curves of heat generation rate after dosing were analyzed. The Maximal Temperature attainable by runaway of the desired Synthetic Reaction (MTSR) under different conditions were calculated for the course hazard evaluation. The results showed that the average reaction heat of mono-nitration was between 169.07~177.11 kJ mol-1, and the special heat of reactant was about 2 kJ kg-1 K-1. Average Ea of second order kinetic was about 30 kJ mol-1, and reaction rate was 10-4 mol s-1 l-1 order of magnitude when the strength of sulphuric acid was about 73.5%. It was also found that when temperature or stirring speed increases, the potential heat accumulation decreases.
Źródło:
Central European Journal of Energetic Materials; 2008, 5, 2; 37-47
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori
Autorzy:
Pan, Ye
Xia, Hengchuan
Lü, Peng
Chen, KePing
Yao, Qin
Chen, Huiqin
Gao, Lu
He, Yuanqing
Wang, Lin
Powiązania:
https://bibliotekanauki.pl/articles/1040486.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
serpin-2
Bombyx mori
bioinformatics
subcellular location
qPCR
Opis:
Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 671-677
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity
Autorzy:
Liu, Dong
Chen, Xing
Xiong, Ren
Ning, Ya
Li, Ping
Peng, Yan
Liu, Ping
Zhao, Yan
Yang, Nan
Zhou, Yuan
Powiązania:
https://bibliotekanauki.pl/articles/1039650.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
dexamethasone
ROCK1
fasudil
non-genomic effects
Opis:
Objective. To explore the effect of dexamethasone (DEX) on monocyte adhesion function and its underlying mechanism. Methods. The effects of DEX and fasudil on adhesion of cultured U937 monocytes to human umbilical vein endothelial cells (HUVEC) following stimulation with phorbol myristate acetate (PMA) were studied; Changes in the Rho-associated coiled-coil protein kinase 1 (ROCK1) protein content and activity were evaluated. Results. DEX and fasudil significantly inhibited U937 cell adhesion rates under PMA stimulation and inhibited ROCK1 activity. Mifepristone (RU-486) and cycloheximide (CHX) did not alter these effects of DEX. Conclusions. DEX interferes with the adhesion function of U937 cells through the inhibition of ROCK1 activity.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 557-560
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stigmasterol blocks cartilage degradation in rabbit model of osteoarthritis
Autorzy:
Chen, Wei-Ping
Yu, Chong
Hu, Peng-Fei
Bao, Jia-Peng
Tang, Jing-Li
Wu, Li-Dong
Powiązania:
https://bibliotekanauki.pl/articles/1039645.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
matrix metalloproteinases
stigmasterol
osteoarthritis
Opis:
Stigmasterol has been shown exihbit anti-osteoarthritic properties in vitro studies. However, the in vivo effects of stigmasterol on cartilage are still unclear. This study investigated the anti-osteoarthritic properties of stigmasterol on cartilage degradation in a rabbit model of osteoarthritis (OA). Twenty rabbits underwent bilateral anterior cruciate ligament transection (ACLT) to induce OA. Five rabbits were used as normal control. Two weeks after operation, the rabbits were randomly divided into two groups. Each group of 10 rabbits received intra-articular injection with 0.3 ml of stigmasterol in left knees and vehicle in right knees, once weekly. Group 1 was killed 6 weeks after ACLT and 2 were sacrificed 9 weeks after ACLT. The knee joints were assessed by gross morphology, histology and gene expression analysis. We found that expression of genes encoding matrix metalloproteinases (MMPs) was significantly higher while tissue inhibitors of metalloproteinase (TIMP)-1 was significantly lower in the both joints of the two OA groups compared to normal contrals. Stigmasterol reduced the cartilage degradation as assessed by histological analysis and markedly suppressed MMPs expression both in group 1 and group 2. Our results suggest that stigmasterol may be considered as a possible therapeutical agent in the treatment of OA.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 537-541
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dft investigation of a high energy density polynitro compound, 2,2’-Bis(trinitromethyl)-5,5’-azo-1,2,3,4- tetrazole
Autorzy:
Lin, H.
Zhu, S. G.
Chen, P. Y.
Li, K.
Li, H. Z.
Peng, X. H.
Powiązania:
https://bibliotekanauki.pl/articles/358030.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
polynitro
electronic structure
thermodynamic properties
crystal structure
detonation performance
stability
Opis:
A novel polynitro compound, 2,2’-bis(trinitromethyl)-5,5’-azo- 1,2,3,4-tetrazole, was designed and investigated at the DFT-B3LYP/6-31G(d) level. Its properties, such as electronic structure, IR spectrum, heat of formation, thermodynamic properties and crystal structure, were predicted. This compound is most likely to crystallize in the P21 space group, and the corresponding cell parameters are Z = 2, a = 5.46 Å, b = 9.72 Å, c = 14.05 Å, α = 90°, β = 90°, γ = 90°. In addition, the detonation velocity and pressure were also estimated by using the empirical Kamlet-Jacobs equations, and were predicted to be 8.28 km/s and 31.61 GPa respectively. The oxygen balance of this compound is +13.79%, which indicates that it could serve as an oxidizer. Bond dissociation energy calculations show that the C(13)-N(21)O2 and C(14)-N(30)O2 bonds are the locations of thermal decomposition and that this compounds meets the thermal stability requirements as an exploitable explosive. Keywords: polynitro, electronic structure, thermodynamic properties, crystal structure, detonation performance, stability.
Źródło:
Central European Journal of Energetic Materials; 2013, 10, 3; 325-338
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Late Glacial and Holocene vegetation and climate history of an alpine wetland on the Qinghai-Tibetan Plateau
Autorzy:
Liu, D. L.
Chen, G.
Lai, Z.
Weis, H.
Zhou, G.
Peng, M.
Powiązania:
https://bibliotekanauki.pl/articles/2059730.pdf
Data publikacji:
2013
Wydawca:
Państwowy Instytut Geologiczny – Państwowy Instytut Badawczy
Tematy:
Late Glacial
Holocene
Qinghai-Tibetan Plateau
alpine wetland
optically stimulated luminescence
pollen analysis
Opis:
This study provides a vegetation and climate history of an alpine wetland in the centre of China’s Qinghai–Tibetan Plateau (QTP) over the last 14.8 ka. High resolution climate proxies include vegetation (pollen), geochemical (Ba, Rb, δ13C) and physical (particle size and magnetic susceptibility) data; optically stimulated luminescence (OSL) and AMS radiocarbon dating were used to reconstruct the environmental changes in the wetland ecosystem. Based on physical and geochemical proxies, the sucession divided into five stratigraphic units encompassing time intervals of 14.76–14.31, 14.31–13.17, 13.17–4.47, 4.47–0.51 and 0.51–0 ka. The average sedimentation rate of all these units was 0.098 mm/a. The study showed that this ecosystem was sensitive to climate change.
Źródło:
Geological Quarterly; 2013, 57, 2; 261--268
1641-7291
Pojawia się w:
Geological Quarterly
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of recombinant expression of Bombyx mori bidensovirus ns1 using a modified vector
Autorzy:
Li, Guohui
Li, Mangmang
Wang, Peng
Hu, Zhaoyang
Yao, Qin
Tang, Qi
Chen, Keping
Powiązania:
https://bibliotekanauki.pl/articles/1039216.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
BmBDV
NS1
baculovirus expression vector
Sf9
egfp
Opis:
ns1 gene of Bombyx mori bidensovirus (BmBDV) consisted of 951 nucleotides encoding a deduced 316-amino aicd protein. In this study, the gene was cloned and fused in frame with a N-terminal 6×His tag under control of the polyhedrin promoter, which was transposed into the mini-attTn7 locus of a modified baculovirus vector. Transfection of Sf-9 cells with the resulting recombinant DNA was performed to prepare recombinant virus and the resultant supernatant of transfection with fluorescent signal was harvested. Western blot analysis revealed that NS1 protein was successfully expressed in Sf9 cells infected with the recombinant virus and was confirmed by LC-MS/MS analysis. Moreover, the expressed NS1 is a phosphorylated protein and the phosphorylation site is Thr-184. These results showed that the activity of BmBDV NS1 may be regulated by phosphorylation.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 787-794
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Preparation of Soluble Cellouronic Acid Sodium Salt by 4-Acetamide-TEMPO Mediated Oxidation of Ultrasound-Pretreated Parenchyma Cellulose from Bagasse Pith
Autorzy:
Gao, X.
Chen, K.
Zhang, H.
Peng, L.
Powiązania:
https://bibliotekanauki.pl/articles/177150.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ultrasonic pretreatment
bagasse pith
parenchyma cellulose
cellouronic acid sodium salt
4-acetamide-TEMPO
Opis:
The parenchyma cellulose isolated from bagasse pith was used as an alternative resource for preparation of water-soluble cellouronic acid sodium salt (CAS). The influence of ultrasound treatment on the cellulose was investigated for obtaining CAS by regioselective oxidization using 4-acetamide-TEMPO and NaClO with NaClO2 as a primary oxidant in an aqueous buffer at pH 6.0. The yield, carboxylate content and polymerization degree (DP) of CAS were measured as a function of ultrasonic power, agitating time and cellulose consistency by an orthogonal test. The ultrasound-treated conditions were further improved by discussion of ultrasonic power, the most important factor influencing the yield and DP. An optimized CAS yield of 72.9% with DP value (DPv) of 212 was found when the ultrasonic strength is 550 W, agitating time is 3 h and cellulose consistency is 2.0%. The oxidation reactivity of cellulose was improved by ultrasonic irradiation, whereas no significant changes in crystallinity of cellulose were measured after ultrasonic treatment. Moreover, the ultrasound treatment has a greater effect on yielding CAS from parenchyma cellulose than from bagasse fibrous’ one. The CAS was further characterized by Fourier transform infrared spectroscopy (FT-IR) and Scanning electron microscopy (SEM).
Źródło:
Archives of Acoustics; 2014, 39, 2; 267-275
0137-5075
Pojawia się w:
Archives of Acoustics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on the Optical and EPR Spectral Data and the Local Structure for the Tetragonal $Nb^{4+}$ Center in Glasses
Autorzy:
Wu, Xiao-Xuan
Yu, Xin-Peng
Zheng, Wen-Chen
Powiązania:
https://bibliotekanauki.pl/articles/1402323.pdf
Data publikacji:
2015-07
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
76.30.He
71.70.Ch
78.40.Pg
78.55.Qr
Opis:
Two optical bands and four EPR (or spin-Hamiltonian) parameters (g factors $g_{∥}$, $g_{⊥}$ and hyperfine structure constants $A_{∥}$, $A_{⊥}$) of the tetragonally-compressed $Nb^{4+}$ octahedral center in glasses are calculated from two methods, the complete diagonalization (of energy matrix) method and the perturbation theory method. Both methods are founded on the two-spin-orbit-parameter model where both the contribution to the spectral data from the spin-orbit parameter of central $d^{n}$ ion and that of ligand ion via covalence effect are considered. The calculated results from both the complete diagonalization (of energy matrix) method and perturbation theory method show reasonable agreement with the experimental values. The signs and possible misprint for the observed hyperfine structure constants $A_{∥}$ and $A_{⊥}$ are suggested, and the local structure of the tetragonal $Nb^{4+}$ center in glasses due to the Jahn-Teller effect is gained from the calculations. The results are discussed.
Źródło:
Acta Physica Polonica A; 2015, 128, 1; 72-75
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrostatic Hazards Assessment of Nitramine Explosives: Resistivity, Charge Accumulation and Discharge Sensitivity
Autorzy:
Peng, Q.
Cao, W.
Zhou, W.
He, Z.
Jiang, W.
Chen, W.
Powiązania:
https://bibliotekanauki.pl/articles/358757.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
nitramine explosives
electrostatic hazards
resistivity
charging characteristics
electrostatic discharge sensitivity
Opis:
The electrostatic hazards of nitramine explosives (RDX, HMX) were assessed in this paper. The resistivities of different particle-size RDX and HMX were tested by a device designed and manufactured according to the standard ISO/IEC 80079-20-2:2016. This work shows that the resistivities of uncompacted RDX and HMX increase as the particle size decreases. Charging characteristics test experiments were also carried out using a so-called sieve method. Using this method, the influence of aperture size on charge accumulation of RDX was studied, and the characteristics of electrostatic accumulation of different particle-size RDX and HMX sieved with 50 mesh standard sieve were compared. The results show that the absolute value of the charge accumulation increases as the mesh number increases (i.e. the aperture size decreases), and increases as the particle size is decreased, indicating that nano-sized RDX and nano-sized HMX accumulate static electricity more easily than conventional micron-sized ones. Finally, the electrostatic discharge (ESD) sensitivity of nano-sized RDX and nano-sized HMX was investigated. Nano-sized nitramine explosives were found to have a higher ESD sensitivity than micron-sized ones.
Źródło:
Central European Journal of Energetic Materials; 2016, 13, 3; 755-769
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Abnormal phase removing method in phase measuring profilometry
Autorzy:
Xu, X.
Cao, Y.
Chen, C.
Cao, S.
Peng, K.
Powiązania:
https://bibliotekanauki.pl/articles/173504.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
information optics
phase measuring profilometry
phase shifting algorithm
phase error
phase auto-extracting
Opis:
An abnormal phase removing method in phase measuring profilometry is proposed. In the five equal shifting phase steps algorithm, the shifting phase might be extracted from the deformed patterns captured by CCD camera. But there may be some errors introduced by a digital fringe projector and CCD camera in these deformed patterns. The impurity of the deformed patterns may lead to four classes of abnormalities when extracting the shifting phase. These abnormalities may cause the wrong shifting phase extraction by which the reconstructed object might be misshapen or anamorphic, or even in failure. By this proposed method, the above abnormalities can be removed, and the shifting phase can be auto-extracted precisely from the impure deformed patterns without knowing its value. Experimental results verify the feasibility and effectiveness of the proposed method.
Źródło:
Optica Applicata; 2017, 47, 1; 85-95
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
HBx and SP1 upregulate DKK1 expression
Autorzy:
Peng, Hong
Li, Yongguo
Liu, Yunzhi
Zhang, Jingnan
Chen, Ke
Huang, Ailong
Tang, Hua
Powiązania:
https://bibliotekanauki.pl/articles/1038681.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
HCC
HBV
DKK1
SP1
Opis:
Numerous evidences suggested that the hepatitis B virus (HBV) was recognized as an important factor in the development of hepatocellular carcinoma (HCC). Dickkopf-1 (DKK1) recently was reported to be involved in the progress of HCC. HBV may regulate DKK1 expression in hematoma carcinogenesis. Here, we demonstrated that HBV could regulate DKK1 promoter activity which resulted in upregulation of its mRNA and protein expression in several HBV existing cell lines, and HBx played a prominent role in this process. Transcription factor binding site search result showed that there is a SP1 site in DKK1 promoter region. Luciferase assay showed that overexpression of SP1 could increase DKK1 promoter activity in a dose dependent manner. Accordingly, siRNA inhibition of SP1 expression reduced DKK1 promoter activity and decreased the expression of DKK1 protein.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 35-39
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Photo-oxidative Aging Behaviors of Acrylic Coatings during UV Irradiation Investigated by Positron Annihilation Spectroscopy
Autorzy:
Zhao, Xipo
Cai, Xiaolin
Liu, Chang
Chen, Ting
He, Chunqing
Fang, Pengfei
Peng, Shaoxian
Powiązania:
https://bibliotekanauki.pl/articles/1033253.pdf
Data publikacji:
2017-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
Acrylic coating
UV irradiation
positron annihilation spectroscopy
Opis:
Slow positron annihilation spectroscopy, contact angle and scanning electron microscope (SEM) were employed to investigate the surface polarity and morphologies as functions of aging time in acrylic coatings during UV-a irradiation. The S parameter plots of each exposed sample are lower than that of the virgin sample in the whole range of E. The S parameter plots become lower as the exposing longer time, indicated that the irradiation generated more polar groups and increased the oxidation layer depth. Combined with surface morphology and the surface energy of acrylic coating, the variation of contact angle, and surface energy, polar component and dispersive component is related with surface polarity and morphologies.
Źródło:
Acta Physica Polonica A; 2017, 132, 5; 1527-1530
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of mechanical flotation cell and cyclonic microbubble flotation column in terms of separation performance for fine graphite
Autorzy:
Bu, X.
Zhang, T.
Chen, Y.
Peng, Y.
Xie, G.
Wu, E.
Powiązania:
https://bibliotekanauki.pl/articles/110791.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
mechanical flotation
column flotation
Fuerstenau upgrading curve
graphite
Opis:
Comparison of flotation performance between the flotation column and mechanical flotation was carried out to promote the grade and economic value of the graphite ore (15.40% ash content). The ash content of the concentrate of the mechanical flotation was 10.77% at the yield of 79.34%. In contrast, the yield of the concentrate of the column flotation was increased to 88.93% with 10.55% ash content. Comparative study of the Fuerstenau upgrading curves indicated that the column flotation was more efficient for cleaning the graphite ore in the presence of the centrifugal force field, nanobubbles (generated by hydrodynamic cavitation), and the thicker froth layer in comparison with the mechanical flotation.
Źródło:
Physicochemical Problems of Mineral Processing; 2018, 54, 3; 732-740
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 43

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