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Wyszukujesz frazę "Chen, Keping" wg kryterium: Wszystkie pola


Wyświetlanie 1-4 z 4
Tytuł:
Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction
Autorzy:
Chen, Huiqing
Li, Yijia
Chen, Keping
Yao, Qin
Li, Guohui
Wang, Lin
Powiązania:
https://bibliotekanauki.pl/articles/1040312.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
calorie restriction
Bombyx mori
two-dimensional gel electrophoresis
proteomic analysis
MALDI-TOF/TOF MS
Opis:
Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 505-511
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of recombinant expression of Bombyx mori bidensovirus ns1 using a modified vector
Autorzy:
Li, Guohui
Li, Mangmang
Wang, Peng
Hu, Zhaoyang
Yao, Qin
Tang, Qi
Chen, Keping
Powiązania:
https://bibliotekanauki.pl/articles/1039216.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
BmBDV
NS1
baculovirus expression vector
Sf9
egfp
Opis:
ns1 gene of Bombyx mori bidensovirus (BmBDV) consisted of 951 nucleotides encoding a deduced 316-amino aicd protein. In this study, the gene was cloned and fused in frame with a N-terminal 6×His tag under control of the polyhedrin promoter, which was transposed into the mini-attTn7 locus of a modified baculovirus vector. Transfection of Sf-9 cells with the resulting recombinant DNA was performed to prepare recombinant virus and the resultant supernatant of transfection with fluorescent signal was harvested. Western blot analysis revealed that NS1 protein was successfully expressed in Sf9 cells infected with the recombinant virus and was confirmed by LC-MS/MS analysis. Moreover, the expressed NS1 is a phosphorylated protein and the phosphorylation site is Thr-184. These results showed that the activity of BmBDV NS1 may be regulated by phosphorylation.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 787-794
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor
Autorzy:
Zhu, Minfeng
Chen, Keping
Wang, Yong
Guo, Zhongjian
Yin, Huijuan
Yao, Qin
Chen, Huiqin
Powiązania:
https://bibliotekanauki.pl/articles/1040735.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
BmAdipoR
bioinformatics study
subcellular localization
real-time quantitative PCR
BmNPV
Opis:
In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences and similar structure to the human adiponectin receptor (AdipoR). Structural analysis of the translated cDNA suggested it encoded a membrane protein with seven transmembrane domains. BmAdipoR was found to be expressed in multiple tissues and highly expressed in Malpighian tubules, fat body and testis. BmNPV (Bombyx mori nucleopolyhedrovirus) bacmid system combined with confocal microscopy revealed that BmAdipoR was targeted to the cell membrane. We also found that infection with BmNPV did not have an effect on BmAdipoR mRNA quantity in the midgut of susceptible Bombyx mori strain (306) at 48 h, but BmAdipoR mRNA quantity increased significantly at 72 h. We concluded that BmAdipoR gene was a membrane protein ubiquitously expressed in Bombyx mori tissues and that its expression was altered by treating with BmNPV.
Źródło:
Acta Biochimica Polonica; 2008, 55, 2; 241-249
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori
Autorzy:
Pan, Ye
Xia, Hengchuan
Lü, Peng
Chen, KePing
Yao, Qin
Chen, Huiqin
Gao, Lu
He, Yuanqing
Wang, Lin
Powiązania:
https://bibliotekanauki.pl/articles/1040486.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
serpin-2
Bombyx mori
bioinformatics
subcellular location
qPCR
Opis:
Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 671-677
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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