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Tytuł:
Enzymatic activity and biotypes of Candida fungi isolated from the surfaces of mobile phones and hands
Autorzy:
Kordecka, A.
Krajewska-Kułak, E.
Łukaszuk, C.
Kraszyński, M.
Kraszyńska, B.
Powiązania:
https://bibliotekanauki.pl/articles/1918454.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Candida
hands
telephone
API Zym
Opis:
Introduction: The secretion of hydrolytic enzymes is a factor facilitating pathogenic fungi invasion into the tissues. Purpose: To assess hydrolytic activity and biotypes of Candida strains isolated from samples collected from the surfaces of mobile phones and the hands of their owners. Materials and methods: The study included 175 mobile telephones and hands. The API ZYM test was used to assess enzymatic activity; biotyping was performed according to Williamson’s classification. Results: Among the strains isolated from hand surfaces, the highest activity was shown for C. albicans (acid phosphatase, esterase), C. glabrata (leucine arylamidase, acid phosphatase, esterase), and C. krusei (acid phosphatase). Of the strains isolated from phone surfaces, the highest activity was shown for C. albicans (leucine arylamidase, acid phosphatase), C. glabrata (esterase, leucine arylamidase, esterase lipase), and C. krusei (acid phosphatase). Biotypes G, B and F were dominant for all types of fungi, both for strains isolated from phones and hand surfaces. Additionally, biotype A was dominant for C. krusei. Conclusions: C. albicans, C. glabrata, and C. krusei showed activity for all hydrolytic enzymes. The strongest correlation between the hydrolytic activity of fungi isolated from hand and phone surfaces was shown for C. albicans.
Źródło:
Progress in Health Sciences; 2017, 7(1); 18-30
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część I. Techniki fenotypowe
Methods for species identification of genus Candida fungi. Part I. Phenotypic techniques
Autorzy:
Gnat, Sebastian
Powiązania:
https://bibliotekanauki.pl/articles/22323965.pdf
Data publikacji:
2022
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
metody fenotypowe
czynniki chorobotwórcze
grzyby chorobotwórcze
identification
phenotypic methods
morphology examination
Opis:
The incidence of Candida spp. infections has increased in recent years due to the widespread use of broad-spectrum antibiotics and the growing numbers of immunocompromised individuals. Despite the predominance of Candida albicans, non-albicans Candida species, such as Candida glabrata, Candida tropicalis, Candida guilliermondii, Candida dubliniensis, Candida parapsilosis, and Candida krusei are emerging as both, colonizers and pathogens, that can cause superficial and systemic infections. Currently, there are various methods for identifying yeasts from clinical samples. Conventional methods for identification of Candida species are based on morphological and physiological attributes. Although phenotypic identification methods are cost effective and practical procedures for routine discrimination of isolates in the clinical microbiology laboratory, the accurate identification of all isolates. Moreover, given the variability in the phenotypic characteristics and other irregularities in the taxonomy of the genus Candida, identification based on traditional methods has become less reliable. Important progress has been made with various studies on the phenotypic characteristics of these species using commercially available identification systems. The aim of this review is to summarize the methods available for identification of Candida species. The first part of the article presents the characteristics of phenotypic methods and quick identification techniques, including systems based on the use of chromogenic media.
Źródło:
Życie Weterynaryjne; 2022, 97, 02; 94-104
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część II. Techniki molekularne
Methods for species identification of genus Candida fungi. Part II. Molecular techniques
Autorzy:
Gnat, Sebastian
Powiązania:
https://bibliotekanauki.pl/articles/22326536.pdf
Data publikacji:
2022
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
metody molekularne
test PCR
metoda multiplex PCR
metoda nested-PCR
metoda real time PCR
fluorescencyjna hybrydyzacja in situ
spektrometria mas MALDI-TOF MS
czynniki chorobotwórcze
grzyby chorobotwórcze
łańcuchowa reakcja polimerazy
piroliza ze spektometrią mas
identification
molecular techniques
MALDI-TOF MS
Opis:
A rapid and accurate identification of the Candida is crucial for clinical treatment of local and systemic candidiasis. Premature diagnosis of invasive fungal infections is problematic because most clinical signs are non-specific and cultures are often negative or become positive too late for the initiation of effective antifungal therapy. Therefore, studies have been performed to improve molecular techniques for the diagnosis of candidiasis. Today, molecular strategies, such as PCR and non-PCR based methods are used to complement conventional laboratory approach. They provide accurate results in much short time of 1.5–3 h. Given the high accuracy and time saving with molecular typing techniques it is likely that most of these methods could improve routine clinical laboratory identification of Candida yeast species. However, further studies are needed for standardization of such procedures. This article presents an overview and discussion on molecular identification methods in the context of their application for the diagnosis of Candida fungi. Particular attention has been focused on the advantages and limitations of the indicated methods and the possibilities of their implementation for routine use in clinical laboratories.
Źródło:
Życie Weterynaryjne; 2022, 97, 03; 167-174
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania nad przydatnością niektórych pożywek używanych w pracowni mykologicznej do wykonania testu filamentacji dla grzybów z rodzaju Candida
Izuchenie prigodnosti nekotorykh pitatelnykh sred upotrebljaemykh v mukologicheskojj laboratorii po vypolnenii testa filamentacii dlja gribkov tipa Candida
Investigations on the usefulness of certain media used in mycological laboratories for filamentation test for Candida fungi
Autorzy:
Pawlik, B.
Budak, A.
Powiązania:
https://bibliotekanauki.pl/articles/2189421.pdf
Data publikacji:
1974
Wydawca:
Polskie Towarzystwo Diagnostyki Laboratoryjnej
Źródło:
Diagnostyka Laboratoryjna; 1974, 10, 2; 147-151
0867-4043
Pojawia się w:
Diagnostyka Laboratoryjna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część III. Systemy automatyczne i testy rzadko wykorzystywane
Methods of species identification of fungi of the genus Candida. Part III. Automated systems and rarely used tests
Autorzy:
Gnat, Sebastian
Powiązania:
https://bibliotekanauki.pl/articles/22354371.pdf
Data publikacji:
2022
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
systemy automatyczne
system Vitek YBC
system Vitek 2 ID-YST
system Abbott Quantum II
test Tween 80
test wykrywania (1,3)-beta-D-glukanu
test wykrywania galaktomannanu
laboratoria weterynaryjne
czynniki chorobotwórcze
grzyby chorobotwórcze
system identyfikacji drobnoustrojów
Candida fungi
diagnosis
automatic systems
rapid methods
veterinary laboratories
Opis:
It is imperative that pathogenic agents are identified accurately to the species level. The implementation of automatic systems for the identification of Candida yeasts is associated with a large number of tests performed by a given unit, which certainly has a further impact on the price of a single test and the use of devices. Hence, automatic systems are widely used in high-throughput laboratories, e.g. in commercial laboratories with a wide range of activities. Veterinary clinics with their own microbiological unit usually use classical identification methods or semi-automatic systems based on biochemical tests for independent interpretation of the results. Nevertheless, the increased number of invasive procedures, inherent in prolonged hospitalization of animals, and the use of broad-spectrum antibiotic therapy, leads to the conclusion that using automatic fungal identification systems in veterinary laboratories is only a matter of time, and their implementation may leave the classical techniques obsolete. This article provides an overview and discussion of automatic systems as well as new, rapid methods of diagnosing Candida fungi. Particular attention has been focused on Vitek YBC, Vitek 2 ID-YST, Abbot Quantum II, and Microbial ID systems.
Źródło:
Życie Weterynaryjne; 2022, 97, 04; 253-257
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biofilm tworzony przez grzyby - struktura, quorum sensing, zmiany morfogenetyczne, oporność na leki
Biofilm caused by fungi - structure, quorum sensing, morphogenetic changes, resistance to drugs
Autorzy:
Nowak, M.
Kurnatowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/2143486.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Candida albicans
zjawisko quorum sensing
biofilmy grzybicze
grzyby
biofilmy
lekoopornosc
leki przeciwgrzybicze
struktura
zmiany morfogenetyczne
Opis:
Formation of fungal biofilms in patients with implanted biomedical prosthesis constitutes very serious clinical problems. The biofilm can lead to dysfunction of implanted material and can be a reservoir for chronic and systemic infections. Numerous investigations demonstrated differences in quantity and structure of biofilms that had been formed by various species of fungi belonged to Candida genus. Stages of biofilm formations had been examined carefully in in vitro conditions. Biofilm formation begin with adhesion of fungi to the surface, microcolonies are formed subsequently. At the end of the process, extracellular material is excreted, and its formula, that is various in different fungi Candida species, contribute to its resistance to antifungal drugs. Farnesol and tyrosol are two quorum-sensing molecules. They are acting inversely, regulating formation of „germ tubes” and influencing morphogenetic conversion between yeast and filamentous forms, which plays a very important role in pathogenicity and formation of biofilm. Drug resistance of fungi from Candida has been shown to create a very important clinical problem. Many experiments in vitro confirm significantly lower activity of antifungal drugs toward Candida biofilm than toward Candida, in the form of planctonic cells. Surprisingly, some non-steroidal anti-inflammatory drugs can inhibit biofilm formation.
Źródło:
Wiadomości Parazytologiczne; 2009, 55, 1; 19-25
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of Candida spp. frequency in the oral cavity ontocenosis of healthy individuals in different age groups
Autorzy:
Szymańska, Jolanta
Wójtowicz, Agnieszka
Malm, Anna
Powiązania:
https://bibliotekanauki.pl/articles/972336.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
fungi
Candida spp
Candida albicans
Opis:
Introduction. Among the microorganisms colonizing the oral cavity ontocenosis, an important role is played by fungi: mainly yeast-like fungi of the Candida genus, and especially by the species C. albicans. Objective. The aim of this study was to evaluate the frequency of oral colonization by Candida species in healthy individuals of different age groups, and to identify the species of the isolates. Materials and method. The fungi were isolated from the oral cavities of 654 healthy individuals of both genders, representing different age groups, and the species of the isolates were determined. Initial identification of the yeast-like fungi was made based on the macroscopic appearance of the colonies on Sabouraud’s medium, and the growth of coloured colonies on ChromAgar Candida medium. API 20 C AUX microtest (bioMerieux) was used to identify the most frequently found species of Candida spp. Results. The frequency of Candida isolation from the oral cavity ontocenosis in healthy individuals of different age groups was assessed as 30.6%. The oral cavity ontocenosis was colonized mainly by the yeast-like fungi of C. albicans species: they were found in 24.5% of the tested population, while the frequency of oral cavity colonization by non-albicans Candida spp. was 6.1%. Seven species of non-albicans Candida spp. were identified: C. glabrata, C. inconspicua, C. famata, C. tropicalis, C. parapsilosis, C. lusitaniae, and C. kefyr. Conclusions. In the Polish population, 30.6% of the oral cavity ontocenosis is colonized by Candida yeast-like fungi, while C. albicans is the fungus that colonizes oral cavity ontocenosis most frequently. The frequency of oral cavity ontocenosis colonization with Candida spp (including C. albicans) increases with age.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2016, 10, 2; 91-94
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characteristics of growth of yeasts and yeast-like fungi on chromogenic medium CHROMagar Candida (GRASO)
Autorzy:
Góralska, K.
Powiązania:
https://bibliotekanauki.pl/articles/2143359.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
growth
yeast
yeast-like fungi
chromogenic medium
CHROMagar Candida medium
fungal infection
diagnosis
therapy
prophylaxis
rapid diagnosis
Candida
medium property
species list
colour
isolate
Opis:
Early detection and correct diagnosis of fungal infection is important for further therapy and prophylaxis. Currently, there are a number of proposals related to rapid diagnostic tests. To those we can include chromogenic media, such as CHROMagar® Candida (GRASO). This medium has been developed to differentiate four species of genus Candida. Its use in laboratory has opened up possibilities for growing not only the genus Candida, but also other fungi. The study suggests that medium CHROMagar® Candida can provide an excellent pre-test, or be used as complementary to biochemical tests. Establishing a full template of colours would enable more efficient and fuller use of the medium properties, especially in the discrimination of the teleomorphic form from anamorphic.
Źródło:
Wiadomości Parazytologiczne; 2011, 57, 3; 143-149
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of electromagnetic waves on susceptibility of fungi of the genus Candida to miconazole
Autorzy:
Kurnatowski, P
Klimiuk, C.
Glowacka, A.
Powiązania:
https://bibliotekanauki.pl/articles/838869.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
miconazole
electromagnetic wave
susceptibility
pathogenic fungi
Candida
fungi
Opis:
Increasing use of elctromagnetic fields (EF) in the treatment of various diseases may have potential impacts on fungi - possible aetiological factors of concomitant mycoses. The aim of this study was to investigate the effects of EF on miconazole susceptibility among fungi of the genus Candida showing confirmed pathogenicity in humans. Fifteen Candida strains obtained from patients were used and their susceptibility to miconazole was determined by diffusion in agar gel. Antifungal activity of miconazole was assessed as minimal inhibitory concentration (MIC) which was calculated for Candida strains not exposed to EF (control) and EF-exposed (3 experiments). In the majority of cases the susceptibility of Candida species to miconazole decreased (higher MICs) after the first week of EF exposure, regardless of the EF's parameters. This was followed by an increase in susceptibility (lower MICs) after the second week of exposure to EF of 2 mT intensity and frequency 3 Hz (experiment I) and an EF of 9 mT intensity and 12.5 Hz frequency (experiment II) relative to control. An increase in susceptibility (lower MICs) was observed in the second week of exposure, whatever the parameters of the EF. The application of low intensity, low frequency EF for a period of at least two weeks may be beneficial in the treatment of mycoses caused by pathogenic fungi of Candida genus.
Źródło:
Annals of Parasitology; 2003, 49, 1
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
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