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    Wyświetlanie 1-16 z 16
    Tytuł:
    Cloning and heterologous expression of candidate DON-inactivating UDP-glucosyltranferases from rice and wheat in yeast.
    Autorzy:
    Schweiger, Wolfgang
    Steiner, Barbara
    Limmongkon, Apinun
    Brunner, Kurt
    Lemmens, Marc
    Berthiller, Franz
    Bürstmayr, Hermann
    Adam, Gerhard
    Powiązania:
    https://bibliotekanauki.pl/articles/2199622.pdf
    Data publikacji:
    2011-12-20
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    deoxynivalenol
    Fusarium graminearum
    phase II detoxification
    rice
    UDP-glucosyltranferase
    wheat
    Opis:
    Fusarium graminearum and related species causing Fusarium head blight of cereals and ear rot of maize produce the trichothecene toxin and virulence factor deoxynivalenol (DON). Plants can detoxify DON to a variable extent into deoxynivalenol-3-O-glucoside (D3G). We have previously reported the DON inactivat- ing glucosyltransferase (UGT) AtUGT73C5 from Arabidopsis thaliana (Poppenberger et al, 2003). Our goal was to identify UGT genes from monocotyledonous crop plants with this enzymatic activity. The two selected rice candidate genes with the highest sequence similarity with AtUGT73C5 were expressed in a toxin sensitive yeast strain but failed to protect against DON. A full length cDNA clone corresponding to a transcript derived fragment (TDF108) from wheat, which was reported to be specifically expressed in wheat genotypes contain- ing the quantitative trait locus Qfhs.ndsu-3BS for Fusarium spreading resistance (Steiner et al, 2009) was reconstructed. Only cDNAs with a few sequence deviations from TF108 could be cloned. However, toxin sensitive yeast strains expressing this wheat UGT cDNA did not show a resistant phenotype. The main diffi- culty in generating full length cDNAs for functional validation by heterologous expression in yeast is the enormous number of the UGT superfamily members in plants, with 107 UGT genes plus some pseudogenes in Arabidopsis thaliana and about 150 putative UGT genes in grasses. We conclude that neither sequence simi- larity nor inducibility are good predictors of substrate specificity.  
    Źródło:
    Plant Breeding and Seed Science; 2011, 64; 105-112
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Education in a Changing Technological Environment
    Autorzy:
    Banse, Gerhard
    Powiązania:
    https://bibliotekanauki.pl/articles/29520295.pdf
    Data publikacji:
    2006-12-31
    Wydawca:
    Wydawnictwo Adam Marszałek
    Tematy:
    information-based society
    technology-based society
    culture of society
    interaction of technology and culture
    social patterns
    IT security
    Opis:
    The main ideas of this article are the following: (1) Education in a knowledge-based society takes place in a technology-based society. Therefore, we must pay attention to the technological possibilities and changes. (2) On the one hand, the technological “reality” forms the prevailing culture. On the other hand, the cultural patterns of action and using influence this technological “reality”, change it, adapt it to the cultural “environment” (interaction of technology and culture). (3) These actual processes of change include two (ideal) forms: (a) the adjustment of existing cultural resp. social patterns to the new technological possibilities (persistence of the “traditions”); (b) the recombination of existing and the appearance or arising of (complete) new cultural resp. social patterns (surprises, mostly no foreseeable/ predictable). (4) Cultural changes are not an automatic resp. direct result of technological possibilities. The use of these possibilities depends on the competencies, the values a. s. o. of the user(s); this is fundamentally influenced by the existing culture. – On this basis, the article at first will show some trends and problems in the current development of ICTs. Secondly some cultural and social implications of these trends will be discussed (examples: “evaluation and selection of a / the technological solution”, “important criteria of IT-security”, “security/safety culture” and “tacit presence”). Some conclusions finish the reflections.
    Źródło:
    The New Educational Review; 2006, 10; 53-62
    1732-6729
    Pojawia się w:
    The New Educational Review
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Embedded Lifelong Learning: Demands on the Integration of Learning into the Daily Routine
    Autorzy:
    Glahn, Christian
    Gassler, Gerhard
    Hug, Theo
    Powiązania:
    https://bibliotekanauki.pl/articles/28409151.pdf
    Data publikacji:
    2005-03-31
    Wydawca:
    Wydawnictwo Adam Marszałek
    Tematy:
    Lifelong learning
    learner’s motivation
    flexibility of learning
    information and communication technologies
    micro activities
    Opis:
    The Research Studio eLearning Environments has developed the “Learning Pulse”. The “Learning Pulse” makes use of a PUSH technology and supports repetitive learning activities by utilizing ICT. The PUSH technology is based on external initiation of a learning activity. This terminology is derived from webapplication programming. This article reflects on the background and the relationship between the “Learning Pulse” and lifelong learning, focusing on aspects of motivation, flexibility, and learner dropouts. In the second part the idea behind the “Learning Pulse” is compared with the settings in which “conventional” self directed learning takes place. Based on these assumptions it is described how the PUSH approach can be used for embedding learning activities into the daily routine of a learner.
    Źródło:
    The New Educational Review; 2005, 5; 243-254
    1732-6729
    Pojawia się w:
    The New Educational Review
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-16 z 16

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