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Wyszukujesz frazę "Acanthamoeba strains" wg kryterium: Wszystkie pola

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    Wyświetlanie 1-8 z 8
    Tytuł:
    Amoebicidal or amoebostatic influence of disinfectants used in health facilities and laboratories on corneal strains of Acanthamoeba
    Autorzy:
    Baltaza, Wanda
    Padzik, Marcin
    Szaflik, Jacek P.
    Dybicz, Monika
    Hendiger, Edyta
    Chomicz, Lidia
    Powiązania:
    https://bibliotekanauki.pl/articles/972267.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    in vitro dynamics
    influence of disinfectants
    Acanthamoeba strains
    Opis:
    Different Acanthamoeba species are amphizoic organisms distributed in wide range of habitats in natural and man-made environments; they are also detected on surfaces of equipment and accessories in health facilities. Some strains of the amoebae are causative agents of the vision-threatening human disease Acanthamoeba keratitis, mainly reported in contact lens wearers. An exceptional high resistance of Acanthamoeba trophozoites and particularly cysts to chemicals, disinfectants and drugs is believed as influencing difficulty resulting in unsuccessful therapeutic management. As Acanthamoeba keratitis is the serious medical problem worldwide, different chemicals with possible activity against environmental and clinical Acanthamoeba strains are tested. In our study, selected disinfectants used in health care settings and laboratories were tested and their efficacy against the corneal strains Acanthamoeba castellanii and A. polyphaga , and environmental A. castellanii Neff strain was assessed. Comparative assessment of results of the assays show that, apart from amoebistatic effects, the disinfectants indicated expected cysticidal efficacy.
    Źródło:
    Annals of Parasitology; 2017, 63, 3; 162-172
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of in vitro dynamics of pathogenic Acanthamoeba strains originating from contact lens wearers with infectious keratitis
    Autorzy:
    Padzik, M.
    Starosciak, B.
    Szaflik, J.P.
    Pietruczuk- Padzik, A.
    Siczek, P.
    Chomicz, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/5537.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Opis:
    Recently, incidents of Acanthamoeba keratitis, the vision-threatening eye disease, are reported with increasing frequency worldwide, particularly in contact lens wearers. In our study, the retrospective assessment of in vitro dynamics of subsequent pathogenic Acanthamoeba isolates cultured at 24°C, detected in Polish contact lens wearers with keratitis is presented and results compared with those of environmental A. castellanii Neff strain. There were delayed the proper diagnosis that influenced prolonged and severe course of this eye disease and treatment difficulties. The corneal material was examined directly to visualize developmental amoeba stages for diagnose verification, microbiologically tested for the specific identification of bacteriae and fungi, and in vitro grown in culture medium in temperature 24°C. Among twenty-six keratitis incidents analyzed, eleven were cases of Acanthamoeba keratitis; in the six of them, Acanthamoeba strains and concomitant bacterial and/or fungal infectious agents were detected. In vitro assays showed variability in population density of several clinical strains in the exponential growth phase expressed in various range of overall amoeba number and different proportion between trophozoites and cysts. The clear influence of temperature on the in vitro cultivation of the amoebae was observed: statistically significant lower population dynamics was revealed by most of pathogenic clinical isolates in comparison with those showed by environmental strain. The in vitro monitoring of dynamics of Acanthamoeba strains isolated from infected eyes may be helpful for diagnostics verification, especially in mixed infectious keratitis.
    Źródło:
    Annals of Parasitology; 2016, 62, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative examination on selected amphizoic amoebae in terms of their in vitro temperature tolerance - a possible indirect marker of potential pathogenicity of Acanthamoeba strains
    Autorzy:
    Baltaza, W.
    Padzik, M.
    Szaflik, J.P.
    Dybicz, M.
    Grochowska, A.
    Kuryłowicz, A.
    Chomicz, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/5852.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Źródło:
    Annals of Parasitology; 2018, 64, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of in vitro effects of selected physical and chemical agents on detected in Poland Acanthamoeba strains - factors of increasing threats for public health
    Autorzy:
    Baltaza, W.
    Padzik, M.
    Szaflik, J.P.
    Perkowski, K.
    Dybicz, M.
    Chomicz, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/6390.pdf
    Data publikacji:
    2019
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Źródło:
    Annals of Parasitology; 2019, 65, 1
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains
    Autorzy:
    Padzik, M.
    Hendiger, E.B.
    Żochowska, A.
    Szczepaniak, J.
    Baltaza, W.
    Pietruczuk-Padzik, A.
    Olędzka, G.
    Chomicz, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2082951.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Acanthamoeba keratitis
    contact lens solutions
    silver/gold nanoparticles
    Acanthamoeba trophozoites
    anti-amoebic in
    vitro effect
    Opis:
    Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 1; 198-202
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of chlorhexidine disinfectant in vitro effect on environmental and ocular Acanthamoeba strains, the amoebic agents of human keratitis - an emerging sight-threatening corneal disease in Poland
    Autorzy:
    Padzik, M.
    Baltaza, W.
    Szaflik, J.P.
    Hendiger, E.
    Dybicz, M.
    Chomicz, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/6211.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Źródło:
    Annals of Parasitology; 2018, 64, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of Acanthamoeba keratitis – a growing threat to human health worldwide
    Autorzy:
    Padzik, M.
    Baltaza, W.
    Conn, D.B.
    Szaflik, J.P.
    Chomicz, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081938.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    in vitro
    Acanthamoeba Keratitis
    A. polyphaga
    T4 genotype
    A. castellanii Neff
    povidone iodine
    chlorhexidine digluconate
    toyocamycin effects
    Opis:
    Introduction. Free-living amoebae, ubiquitous in outer environments, in predisposing circumstances may exist as parasites, infectious agents of Acanthamoeba keratitis. In recent decades, the vision-threatening corneal infection is a growing human health threat worldwide, including Poland. The applied therapy is often ineffective due to diagnostic mistakes, various pathogenicity of Acanthamoeba strains and high resistance of cysts to drugs; many agents with possible anti-amoebic activity are still being tested. In the presented study, selected chemicals are investigated in terms of their in vitro effect on corneal and environmental Acanthamoeba strains. Materials and method. Samples of a corneal isolate from a patient with severe Acanthamoeba keratitis,of assessed on the basis of genotype associations of 18S rRNA and the type strain, Acanthamoeba castellanii Neff cultivated in bacteria-free condition, were exposed to povidone iodine, chlorhexidine digluconate or toyocamycin. In vitro population dynamics of the strains were monitored and compared to those of control cultures. Results. All chemicals showed anti-amoebic effects with different degrees of effectiveness. Significant differences were observed in the in vitro population dynamics, and the morpho-physiological status of A. castellanii Neff T4 and corneal strains determined as A. polyphaga T4 genotype, exposed to povidone iodine or toyocamycin, in comparison with chlorhexidine taken as reference. Conclusions. Time-dependent amoebstatic in vitro effects were demonstrated for all agents, in particular, the results of assays with povidone iodine are promising. No significant stimulation of encystation appeared; however, as cysticidal efficacy of chemicals is expected, complementary research is needed on different Acanthamoeba strains with modified agent concentrations and method application.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 725-731
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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