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Tytuł:
Investigating Mechanisms of Radiation-Induced DNA Damage Using Low-Energy Photons
Autorzy:
Folkard, M.
Prise, K. M.
Powiązania:
https://bibliotekanauki.pl/articles/2044651.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.-a
Opis:
Central to any mechanistic biophysical model of radiation damage to DNA is the relationship between the amount and distribution of energy deposited in the DNA helix and the subsequent production of DNA damage. It is now clear from a number of studies that the minimum energy required to produce bond breaks in DNA is significantly lower than might be expected. For example, some biophysical models have assumed that it takes several 10s of eV to produce a double-strand break in DNA. However, using low-energy photons, we have shown that energy depositions as low as 7 eV can induce double-strand break and that this is enhanced when the DNA is hydrated, showing that free radical damage is also important.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 265-271
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Single Proton Hit Facility at the IFJ PAN in Cracow
Autorzy:
Polak, W.
Lekki, J.
Veselov, O.
Stachura, Z.
Styczeń, J.
Powiązania:
https://bibliotekanauki.pl/articles/2044772.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.Gi
87.50.-a
Opis:
This work presents the final development stage and optimization results of the single proton hit facility in Cracow. The setup parameters: proton beam energy 2 MeV, hit resolution 30μm, intensity ≈1000 proton/s, proton registration efficiency 100%, blanking efficiency 99.8%. On-line observation allows targeting cells inside a Petri dish mounted at a 3D precise moving stage. To target a particular cell a semiautomatic cell visualization and recognition system is used.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 417-420
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Understanding Radiation Damage to Cells Using Microbeams
Autorzy:
Folkard, M.
Prise, K. M.
Shao, C.
Gilchrist, S.
Schettino, G.
Michette, A. G.
Vojnovic, B.
Powiązania:
https://bibliotekanauki.pl/articles/2044649.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
41.75.Ak
87.50.-a
Opis:
Cellular micro-irradiation techniques provide unique experimental opportunities for understanding how ionizing radiation interacts with living cells and tissues. Using microbeams, it is possible to deliver precise doses of radiation to selected individual cells, or sub-cellular targets in vitro. This technique continues to be applied to the investigation of a number of phenomena currently of great interest to the radiobiological community. In particular, it is the study of so-called "non-targeted" effects (where cells are seen to respond indirectly to ionizing radiation) that are benefiting most from the use of microbeam approaches.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 257-264
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The GSI Heavy Ion Microbeam: A Tool for the Investigation of Cellular Response to High LET Radiations
Autorzy:
Barberet, Ph.
Heiss, M.
Du, G.
Fischer, B. E.
Taucher-Scholz, G.
Powiązania:
https://bibliotekanauki.pl/articles/2044671.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
41.75.Ak
87.50.-a
Opis:
Since the mid 1990's, an increasing number of charged particle microbeams have been designed to deliver a preset number of ions to individual living cells with the micron resolution. These tools provide a powerful technique to investigate the cellular response to low doses of radiations. During the last years, the single ion hit facility in operation on the GSI microbeam since 1987 has been upgraded for the irradiation of individual living cells in vitro. This setup presents two main peculiarities compared to the microbeams used up to now for cell irradiation. First, the beam's micrometric size is obtained by magnetic focusing and not by a simple collimation. This allows obtaining a smaller beam spot, a better defined linear energy transfer, and a high irradiation throughput. Then, the GSI microbeam is able to focus ions from carbon to uranium with energies between 1.4 MeV/u to 11.4 MeV/u. The range of accessible linear energy transfer is thus considerably extended compared to light ions microbeam in operation today. The design of the GSI microbeam is described, including the beam control, the online cell localisation, the cell dish designed specifically for microbeam irradiation, and the cell irradiation procedures. Experimental tests performed to check the global aiming accuracy as well as the first cellular irradiations are presented.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 329-334
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability
Autorzy:
Miszczyk, J.
Panek, A.
Rawojć, K.
Swakoń, J.
Prasanna, P.
Rydygier, M.
Gałaś, A.
Powiązania:
https://bibliotekanauki.pl/articles/1398817.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.-a
87.53.-j
87.53.Bn
Opis:
Particle radiotherapy such as the one using proton beams, provides a successful treatment approach in many cancer types. However, the cellular and molecular mechanisms by which proton irradiation induces cell death, particularly in a human peripheral blood lymphocyte model has not been examined in detail. Comparative studies of the biological effects, such as cell death, of particle therapy versus conventional X-rays treatment are of utmost importance. Here, we compared the viability of human peripheral blood lymphocyte following in vitro irradiation with protons (therapeutic 60 MeV proton beam) and photon beam (250 kV, X-rays), by applying separate doses within the range of 0.3-4.0 Gy. Cell viability was assessed 1 and 4 h after irradiation with protons and X-rays by the FITC-Annexin V labelling procedure (Apoptotic & Necrotic & Healthy Cells Quantification Kit, Biotium). Results showed that irradiation with both radiation types reduced the number of viable cells in a dose-dependent manner, as assessed as a function of the duration of post-irradiation time. Protons proved more fatal to the cells treated than X-ray photons. This demonstrates a difference in cell viability after irradiation with protons and photons in a human peripheral blood lymphocyte model.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 222-225
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Pulsed High-Voltage Stimulation οn Pholiota Nameko Mushroom Yield
Autorzy:
Takaki, K.
Yamazaki, N.
Mukaigawa, S.
Fujiwara, T.
Kofujita, H.
Takahasi, K.
Narimatsu, M.
Nagane, K.
Powiązania:
https://bibliotekanauki.pl/articles/1807916.pdf
Data publikacji:
2009-06
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.-a
52.90.+z
82.39.Rt
Opis:
A pulsed high voltage was applied to logs for mushroom cultivation to investigate an effect of pulse high voltage on mushroom growth. Inductive energy storage system was utilized to construct a pulsed power generator with compact size. Copper fuse of 0.03 mm diameter was used as an opening switch to interrupt large circuit current in short time. The voltage charged in primary energy storage capacitor was multiplied using secondary energy storage inductor. The output voltage of the pulsed power generator was 120 kV with 100 ns pulse width at 14 kV charging voltage of 0.22 μF primary energy storage capacitor and 15 μH secondary energy storage inductor. This pulsed high voltage was applied to 90 cm length logs inoculated Pholiota nameko fungus. The yield of Pholiota nameko mushrooms was successfully improved with the pulse voltage stimulation. The total weight of the cropped Pholiota nameko after the pulse voltage stimulation was 6.3 kg for fifteen logs and this value was 1.5 times larger than 4.3 kg of total weight without the stimulation. The number of logs with lower than 0.27 kg cropped Pholiota nameko weight which was an average value for control group was only one in fifteen, whereas four logs in control group showed almost zero weight of cropped Pholiota nameko.
Źródło:
Acta Physica Polonica A; 2009, 115, 6; 1062-1065
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of magnetite composite on the amount of double strand breaks induced with X-rays
Autorzy:
Wiecheć, A.
Nowicka, K.
Błażewicz, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1065182.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.-a
87.53.-j
81.05.Mh
81.05.Ni
81.05.Pj
81.05.Qk
75.50.Bb
Opis:
The aim of this study was to find out if polylactide (PLA) modified with magnetite might affect the amount of DNA double strand breaks induced with X-rays. The human osteosarcoma cells (MG63) were seeded on the polystyrene cell culture dishes (PS), PLA and PLA modified with magnetite substrates. The double strand breaks were analyzed after X-ray irradiation (dose rate 2 Gy/min), in the first day of culturing. The number of double strand breaks increased in the PLA modified with magnetite, for example after 1 Gy of X-rays irradiation, double strand breaks/cell equaled: 24.5 vs. 17.5 and 17.3, for PLA modified with magnetite vs. PLA and PS, p < 0.0003. We conclude that PLA modified with magnetite changed the number of double strand breaks induced with X-rays. However, more research is needed to confirm that such composite might be considered as radiosensitizer in radiotherapy.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 174-175
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The UV-C induced cell death in human malignant melanoma and normal fibroblasts
Autorzy:
Panek, A.
Wiecheć, A.
Powiązania:
https://bibliotekanauki.pl/articles/1065193.pdf
Data publikacji:
2016-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.W-
87.14.gk
Opis:
Differences in cellular death between melanoma (Me45) cells and fibroblasts (CCL-110) were investigated after irradiation with UV-C (1.5-15 J/m²) and incubation for up to 48 h. The role of DNA double strand breaks in this process was assessed. Decrease of the Me45 cells viability began about 6 h after irradiation. The fibroblasts viability negatively correlated with the dose applied, since necrosis within this cell population began immediately after irradiation. The enhanced apoptosis of fibroblasts was observed between 6 and 24 h, while for melanoma cells, high level of apoptotic cells was still detected after 48 h. Statistically significant correlation between the percentage of apoptotic cells and DSBs was estimated for both cell lines. The melanoma cells responded differently to the UV-C radiation than did the fibroblasts. These differences were explained by deficiency of the necrotic processes as well as the delay of apoptotic melanoma response to UV-C damage.
Źródło:
Acta Physica Polonica A; 2016, 129, 2; 172-173
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Unfocused/Weakly Focused Pressure Pulse Sources for Pain Therapy: Measurements in Water and in a Dry Test Bench
Autorzy:
Ueberle, F.
Jamshidi-Rad, A.
Powiązania:
https://bibliotekanauki.pl/articles/1383801.pdf
Data publikacji:
2015-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
43.80.Gx
43.20.Px
87.50.yt
87.50.yk
Opis:
Unfocused/weakly focused pressure pulses (UPP) are generated by an "air-gun" like mechanism, with a projectile accelerated by pressurized air and impinging on a metal applicator. They were introduced in 1998 for the treatment of orthopedic soft tissue pain. The patient side of the applicator (a circular piston of 6-30 mm diameter) releases single pressure pulses of 2-10 MPa and 4-5 μs duration. Up today, there is no standard for the measurement of UPP sources (UPPS), so data are often reported on the basis of the focused lithotripter standard IEC61846. The purpose of this research is to establish methods to reliably measure UPPS acoustic parameters and establish a parameter set based on definitions from focused lithotripsy sources as applicable. Therefore, acoustic characteristics and wave fields of the pressure pulses of UPPS from different manufacturers were measured in a water bath and in a dry test bench. It was demonstrated by comparison with optical hydrophones that piezoelectric hydrophones are appropriate for the measurements of UPP. For on-axis measurements at a fixed distance, measurements in the dry test bench can replace water-bath measurements. Additionally, the dry test bench allows for reliable results at pulse rates >1 Hz.
Źródło:
Acta Physica Polonica A; 2015, 127, 1; 135-137
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synergistic Effect of ELF-Magnetic Field and IR-Radiation on Tinnitus Patients
Autorzy:
Hojan-Jezierska, D.
Pankowska, M.
Wicher, A.
Szymiec, E.
Kraśnik, M.
Knapkiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/1490490.pdf
Data publikacji:
2012-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.50.C-
87.50.ct
Opis:
The LED light therapy and magnetostimulation is an innovative method of treatment used in Viofor JPS System. Simultaneous application of both types of electromagnetic radiation increases the therapy effectiveness by the synergy effect. It is recommended in analgesic, anti-inflammatory treatment, and to support immune system of the organism. The mechanism of biological effect of variable magnetic fields and light are of similar nature at the tissue level. When applying magnetostimulation and light together, it shortens the time of therapy. The LED light therapy and magnetostimulation is supposed to be an alternative supporting method to therapies used to treat tinnitus so far.
Źródło:
Acta Physica Polonica A; 2012, 121, 1A; A-096-A-099
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-10 z 51

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