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Wyświetlanie 1-15 z 27
Tytuł:
The importance of molecular identification methods of mosquitoes in epidemiology of vector-borne diseases
Autorzy:
Rydzanicz, K.
Lonc, E.
Becker, N.
Powiązania:
https://bibliotekanauki.pl/articles/5621.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
vector-borne disease
Culex
Anopheles
molecular identification
molecular method
mosquito
epidemiology
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Progress in the molecular methods for the detection and genetic characterization of Cryptosporidium in water samples
Autorzy:
Skotarczak, B
Powiązania:
https://bibliotekanauki.pl/articles/52184.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
protozoan parasite
Cryptosporidium
genetic characteristics
molecular method
detection
water sample
food-borne way
oocyst
infection control
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 1-8
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Advanced methods of bacteriological identification in a clinical microbiology laboratory
Autorzy:
Zukowska, M.E.
Powiązania:
https://bibliotekanauki.pl/articles/2098275.pdf
Data publikacji:
2020
Wydawca:
Instytut Medycyny Wsi
Tematy:
clinical microbiology
molecular method
modern method
multiplex polymerase chain reaction
real-time polymerase chain reaction
next generation sequencing
MALDI-TOF mass spectrometry
Opis:
Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory’s apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2021, 15, 2; 68-72
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykrywanie bakterii Salmonella metodami molekularnymi w produktach żywnościowych
Detection of Salmonella sp. in food using molecular methods
Autorzy:
Misiewicz, A.
Goncerzewicz, A.
Powiązania:
https://bibliotekanauki.pl/articles/796639.pdf
Data publikacji:
2013
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
produkty spozywcze
bakterie
Salmonella
wykrywanie drobnoustrojow
metody molekularne
lancuchowa reakcja polimerazy
food product
bacteria
microorganism detection
molecular method
polymerase chain reaction
Opis:
Bakterie z rodzaju Salmonella są najbardziej znanymi bakteriami patogennymi występującymi w przemyśle spożywczym. Powodują zakażenia prawie wszystkich produktów żywnościowych – od mięsnych, mlecznych, jajecznych po rośliny oleiste i pasze. Wykrycie i identyfi kacja bakterii Salmonella na podstawie tradycyjnej metody mikrobiologicznej, zgodnie z normą PN-EN 6579:2003, jest ciągle powszechnie stosowane w laboratoriach. Analiza ta jest czaso- i pracochłonna, jej wykonanie trwa około 5–7 dni. Wykorzystanie nowoczesnych technik biologii molekularnej, z etapem namnożenia i izolacji DNA, do uzyskania końcowego wyniku trwa znacznie krócej. W niniejszej pracy zastosowano metodę Real-Time PCR i klasyczny PCR do identyfi kacji bakterii Salmonella w różnych produktach żywnościowych. Określono czas potrzebny do wykonania tej analizy molekularnej. Do reakcji Real-Time PCR wykorzystano komercyjny kit. Klasyczną reakcję PCR prowadzono z użyciem starterów Sal465Li Sal142F, uzyskując właściwy produkt o wielkości 343 pz. We wszystkich badanych próbkach wykryto bakterie Salmonella. Wynik analiz molekularnych uzyskano w ciągu 21–25 godzin.
Pathogenic bacteria of Salmonella genus are the most common infections in food industry. They might to contaminate wide range of products, protein food e.g. meat, milk food, eggs and egg foods, plants and its preserves, fodder and feeds. Detection and identifi cation of Salmonella sp. are made using traditional methods corresponding with standard PN-EN 6579:2003. That method is commonly used in the microbiological laboratories its time consuming and laborious analysis. Using a modern molecular biology techniques allow to confi rm the Salmonella infections in 24-hours with enrichment and isolation steps. In our study were used Real-Time PCR and traditional PCR techniques to detect Salmonella pathogens from various foods. In addition we checked time of molecular analysis. In the study was used commercial kit to Real-Time PCR analysis and primer set Sal465L, Sal142F with are based on characteristic and solid genomic fragments of Salmonella genus. In every experiment we got positive results for food contaminated by Salmonella. The results were obtained in 21–25 hours.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2013, 573
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs
Autorzy:
Jablonski, A.
Zebek, S.
Kolacz, R.
Pejsak, Z.
Powiązania:
https://bibliotekanauki.pl/articles/30649.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
animal disease
pig
animal infection
epidemiology
Haemophilus parasuis
genotyping
polymerase chain reaction
virulence
diagnosis
microorganism
DNA fragment
electrophoretic separation
environmental factor
molecular method
Opis:
Haemophilus parasuis belongs to opportunistic microorganisms of undefined virulence. The purpose of the studies was to compare suitability of PCR/RFLP in our modification and ERIC PCR for epidemiological study of domestic strains of H. parasuis. The results were evaluated taking into account two different aspects: suitability of the tests for isolating the highest possible number of clone groups and subjective evaluation of the method judged with respect to the following criteria: difficulty, availability of equipment and reagents as well as time and cost of the study. The results obtained in the present study show that the two methods used for typing of H. parasuis had high discriminatory power. Taking into account this parameter it can be concluded that ERIC PCR is more suitable than PCR/RFLP. This justifies the use of ERIC PCR for routine epidemiological analyses of mentioned pathogen. Taking into account the complexity of method used, ERIC-PCR based on random amplification of DNA, proved to be comparable to PCR/RFLP. The last mentioned technique is relatively less expensive and labour-consuming, especially when diagnostic PCR method is used for the epidemiological studies.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Theoretical study of kinetic isotope effects on hydrogen abstraction reactions
Autorzy:
Kurosaki, Y.
Powiązania:
https://bibliotekanauki.pl/articles/147000.pdf
Data publikacji:
2002
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
ab initio molecular orbital method
hyrogen abstraction reaction
potential energy surface
secondary isotope effect
transition state theory
tunneling
Opis:
Effects of isotopic substitutions of "spectator" hydrogens on rate constants have been theoretically examined for the CH3 + H2 ' CH4 + H (I) and C2H + H2 ' C2H2 + H (II) reactions using variational transition state theory with the multidimensional semiclassical tunneling correction. A small but significant secondary isotope effect was found for reaction (I) but almost no isotope effect was found for reaction (II). This is because the potential energy surface for reaction (II) has an "early" character.
Źródło:
Nukleonika; 2002, 47,suppl.1; 81-88
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermal Expansion of Explosive Molecular Crystals: Anisotropy and Molecular Stacking
Autorzy:
Qian, W.
Zhang, C.
Xiong, Y.
Zong, H.
Zhang, W.
Shu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/358262.pdf
Data publikacji:
2014
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
energetic material
anisotropic thermal expansion
molecular stacking
molecular dynamics simulation
density functional theory method
Opis:
Molecular dynamics simulations of three typical explosive crystals, octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), 1,1-diamino-2,2- dinitroethene (FOX-7) and 1,3,5-triamino-2,4,6-trinitrobenzene (TATB), were carried out under NPT ensemble and selected force field. The equilibrium structures at elevated temperatures were obtained, which show that the stacking behaviour of the molecules does not change with temperature. The coefficient of thermal expansion (CTE) values were calculated by linear fitting methods, and the results show that the CTE values are close to the experimental results and are anisotropic. The total energies of the cells expanding along each single crystallographic axis were calculated by the periodic density functional theory method, indicating that the energy change rates are anisotropic, and correlation equations of the energy change vs. CTE values were established. The essence of the anisotropy of the explosive crystal’s thermal expansion was compared and elucidated.
Źródło:
Central European Journal of Energetic Materials; 2014, 11, 1; 59-81
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial, DNA photocleavage and molecular docking studies of newly prepared Schiff-based macrocyclic complexes
Autorzy:
Mishra, Purti
Sethi, Pooja
Kumar Ramasamy, Selva
Saini, Adesh K.
Singh Tuli, Hardeep
Mittal, Divya
Trehan, Aarti
Powiązania:
https://bibliotekanauki.pl/articles/40422849.pdf
Data publikacji:
2024-03-30
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:
anti-bacterial
DFT
DNA photocleavage
molecular docking
template method
Opis:
Introduction and aim. At present, several microbial diseases are prominent and of concern worldwide. The intent of this study was to examine the antibacterial potential of newly synthesized tetradentate macrocyclic complexes against different bacterial strains. The macrocyclic scaffold has gained attention as a biologically active class of supramolecular chemistry due to its unique properties and ability to target various microorganisms. Thus, the goal of the present study was to develop a series of biologically active transition metal-based macrocycles. Material and methods. All macrocyclic compounds were synthesized by a template method and validated by molar conductivity, elemental studies, and spectral and magnetic studies. Antibacterial activities of all metal complexes were evaluated against Escherichia coli (MTCC 739) and Staphylococcus aureus (MTCC 731) bacterial strains by taking ampicillin as a standard reference drug. DNA photocleavage potential was explored using agarose gel electrophoresis. Results. Results revealed the formation of novel macrocyclic complexes via tetra nitrogen bond trapping of metals. Copper complexes have strong potential against S. aureus bacteria as copper and nickel both show good DNA photocleavage potential. Conclusion. The findings endorse the biomedical relevance of these macrocyclic scaffolds, suggesting avenues for further exploration in targeted drug delivery and potential clinical applications. The proposed octahedral geometry for the complexes enhances our understanding of their structural aspects. This research contributes substantively to the field, laying the foundation for future investigations in advanced antimicrobial design and application.
Źródło:
European Journal of Clinical and Experimental Medicine; 2024, 22, 1; 154-163
2544-2406
2544-1361
Pojawia się w:
European Journal of Clinical and Experimental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrostatics in computer-aided drug design
Autorzy:
Naray-Szabo, G.
Matyus, P.
Powiązania:
https://bibliotekanauki.pl/articles/1953955.pdf
Data publikacji:
1998
Wydawca:
Politechnika Gdańska
Tematy:
electrostatics
drug design
molecular electrostatic potential
molecular electrostatic field
similarity
pharmacophore
ComFa method
protein-ligand interaction
Opis:
Hydrogen bonds and charge-charge interactions, determined by molecular electrostatics, play essential role in biopolymer-ligand associations. Accordingly, electrostatics is crucial in the qualitative and quantitative characterisation of the binding of drugs to their target molecules. In the following, we will give an account on the role of molecular electrostatics in a drug design, laying emphasis on our own work. We will survey the most important computation methods of molecular electrostatic potentials, then outline basic aspects of molecular recognition: steric, electrostatic and hydrophobic complementarity. On the basis of the complementarity, we will also define molecular similarity and discuss various applications of these concepts to the treatment of protein-ligand interactions and a rational drug design. Special attention will be paid to a receptor mapping and to a comparative molecular field analysis, with some our recent applications. A further important point will be the molecular electrostatic field (potential gradient) as a hydrophobicity measure. We will argue that the hydrophobic complementarity and similarity can be treated on the basis of matching regions of the interacting molecules that are characterised by a similar magnitude of the electrostatic field. The concept of the electrostatic complementarity will be extended to enzyme-substrate interactions, providing a firm basis for the quantitative estimation of catalytic rate enhancement.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 1998, 2, 4; 551-562
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of the molecular dynamics method for modelling of mass transfer on the border of Ni-Al bimetal
Zastosowanie metody dynamiki molekularnej do modelowania transferu masy na granicy bimetalu Ni-Al
Autorzy:
Starostenkov, M.
Demina, I.
Popova, G.
Denisova, N.
Smolarz, A.
Powiązania:
https://bibliotekanauki.pl/articles/408811.pdf
Data publikacji:
2012
Wydawca:
Politechnika Lubelska. Wydawnictwo Politechniki Lubelskiej
Tematy:
modelowanie komputerowe
proces dyfuzji
wakans
metoda dynamiki molekularnej
atom międzywęzłowy
sieć krystaliczna
transport masy
method of computer modelling
diffusion process
vacancy
method of molecular dynamics
internodal atom
crystalline lattice
mass transfer
Opis:
One of the basic issues of examination of process of interaction of two-component alloys is the study of the mass transfer process in the presence of dot flaws: internodal atoms and vacancies. The study of this process in real experiments is impossible; therefore it is reasonable to apply computer modelling. The authors present a model of Ni-Al bimetal diffusion process produced by the method of molecular dynamics.
Jednym z podstawowych zagadnień analizy procesu interakcji stopów dwuskładnikowych jest badanie procesu wymiany masy w obecności defektów punktowych: atomów międzywęzłowych i wakansów. Badania tego procesu w rzeczywistych eksperymentach jest niemożliwe, dlatego uzasadnione jest zastosowanie modelowania komputerowego. Autorzy przedstawiają model procesu dyfuzji w bimetalu Ni-Al stworzony metodą dynamiki molekularnej.
Źródło:
Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska; 2012, 1; 36-38
2083-0157
2391-6761
Pojawia się w:
Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prediction of the Density of Energetic Co-crystals: a Way to Design High Performance Energetic Materials
Autorzy:
Zohari, Narges
Mohammadkhani, Faezeh Ghiasvand
Powiązania:
https://bibliotekanauki.pl/articles/357956.pdf
Data publikacji:
2020
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
energetic co-crystals
density
MLR method
artificial neural network
molecular design
Opis:
For designing a new energetic material with good performance, a knowledge of its density is required. In this study, the relationship between the densities of energetic co-crystals and their molecular structures was examined through a quantitative structure-property relationship (QSPR) method. The methodology of this research provides a new model which can relate the density of an energetic co-crystal to several molecular structural descriptors, which are calculated by Dragon software. It is indicated that the density of a co-crystal is a function of sp, OB, DU, nAT, as well as several non-additive structural parameters. The new recommended correlation was derived on the basis of the experimental densities of 50 co-crystals with various structures as a training set. The R2 or determination coefficient of the derived correlation was 0.937. This correlation provided a suitable estimate for a further 12 energetic co-crystals as a test set. Meanwhile, the predictive ability of the correlation was investigated through a cross validation method. Moreover, the new model has more reliability and performance for predicting the densities of energetic co-crystals compared to a previous one which was based on an artificial neural network approach. As a matter of fact, designing novel energetic co-crystals is possible by utilising the proposed method.
Źródło:
Central European Journal of Energetic Materials; 2020, 17, 1; 31-48
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prediction of binding affinities for DNA intercalators by molecular dynamics simulations
Autorzy:
Mazerski, J.
Powiązania:
https://bibliotekanauki.pl/articles/1965824.pdf
Data publikacji:
2001
Wydawca:
Politechnika Gdańska
Tematy:
molecular modelling
free energy of binding
intercalation into DNA
linear responce method
free energy perturbation method
Opis:
The computer modelling and simulation methods are widely used in rational drug design to obtain information necessary for understanding interactions between a ligand (drug molecule) and its cellular macromolecular target on molecular level. The determination of free energies of binding for ligand-target complexes is one of the crucial points in those studies. In recent years several methods have been proposed to solve this problem. The majority of them use molecular dynamics (MD) simulations. Two, most popular methods: (i) a free energy perturbation method (FEP), and (ii) a linear response (LR) method, are shortly presented in this paper together with their limitations and advantages. In this work I presented the first attempt to use LR approach to 10 anti-tumour agents able to intercalate into DNA. The LR relationship obtained in the present study indicated that in the system studied the electrostatic term has no influence on the free energy of binding. The relationship is now successfully used in our research group in further molecular modelling studies concerning DNA intercalators with similar structure.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2001, 5, 3; 301-310
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
2D simulations of liquid percolation through model porous media: preliminary MD and DPD results
Autorzy:
Rychcik, M.
Bośko, J.
Rybicki, J.
Alda, W.
Dzwinel, W.
Mancini, G.
Fioretti, L.
Powiązania:
https://bibliotekanauki.pl/articles/1964080.pdf
Data publikacji:
2001
Wydawca:
Politechnika Gdańska
Tematy:
2D flow simulations
particle methods
molecular dynamics
dissipative particle dynamics method
Opis:
In the paper we make a short overview of computer models based on particle approach, which can be suitable for the simulation of fluid flow through porous media. We concentrate on Molecular Dynamics (MD) and Dissipative Particle Dynamics (DPD) methods. We describe main features of our simulation programs, and present and discuss preliminary results of MD and DPD simulations of 2D fluid flow through a simple model rigid porous media. The paper aims at the evaluation of the applicability of MD and DPD methods for simulations of liquid flows in media of complicated geometry.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2001, 5, 1; 85-97
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Novel Correlation for Predicting the Density of Tetrazole–N-oxide Salts as Green Energetic Materials through Their Molecular Structure
Autorzy:
Zohari, N.
Bajestani, I. R.
Powiązania:
https://bibliotekanauki.pl/articles/358082.pdf
Data publikacji:
2018
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
tetrazole-N-oxide energetic compounds
density
correlation
multiple linear regression method
molecular structure
Opis:
In this study, a new and reliable model is derived for predicting the density of tetrazole N-oxide salts using molecular structure descriptors through multiple linear regression methods (MLR). The proposed model is based on the elemental, functional group and type of molecule descriptors. The coefficient of determination (R2) of the model was 0.9321 for 36 energetic tetrazole N-oxides with different molecular structures as an exploratoryset. The predictive ability of this model has been checked using a cross validation method (Q2 LOO = 0.9325 and Q2 LMO = 0.9324). The new correlation had a root mean square deviation (RMSD) of 0.033 g·cm−3 and an average absolute deviation (AAD) of 0.025 g·cm−3. This correlation also gave good predicted results for a further 10 energetic tetrazole N-oxides as a test set. The new reliable model can also be appliedfor designing novel energetic tetrazole N-oxides.
Źródło:
Central European Journal of Energetic Materials; 2018, 15, 4; 629-651
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis
Autorzy:
Przybylska, A.
Fiedler, Z.
Obrepalska-Steplowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/66435.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
PCR-RFLP method
Frankliniella occidentalis
Frankliniella intonsa
Frankliniella pallida
Frankliniella tenuicornis
thrip
molecular diagnostics
pest control
detection
Opis:
Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on the quarantined list of the European Plant Protection Organization. The rapid detection and differentiation between more and less harmful Frankliniella species is, therefore, important in order to combat the pests at the time of their appearance. In this study, we have undertaken to develop a method of detecting F. occidentalis, F. intonsa, F. pallida, and F. tenuicornis. The protocol is based on PCR amplification of ITS1 rDNA fragments of these insects using universal primers pair giving products of slightly distinct length for studied insects. Restriction enzymes digestion which is easy to interpret, allows for visible differentiation of all these Frankliniella species. The method was shown to be species-specific and sensitive. Even single specimens in either the larvae or adult stage could be distinguished.
Źródło:
Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
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