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    Wyświetlanie 1-5 z 5
    Tytuł:
    Disturbances of mouse pregnancy after injection of Ascaris homogenate during early organogenesis
    Autorzy:
    Blaszkowska, J
    Powiązania:
    https://bibliotekanauki.pl/articles/838767.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pregnancy
    teratogenicity
    tegumental homogenate
    mouse
    mice
    Ascaris
    Ascaris suum
    organogenesis
    homogenate
    embryotoxicity
    Opis:
    Administration of the Ascaris tegumental homogenate (0.6-1.2 g of Ascaris proteins/kg/day) at a early stage of organogenesis (5-9 days of pregnancy) had a harmful effect upon the developing mouse fetuses. It has been found that injection of the homogenate did not delay or prevent implantation, but causes a high rate of intrauterine deaths. The Ascaris homogenate significantly decreased the number of live fetuses per litter, increased the frequency of litter resorption, produced a delay in bone formation and induced pathological changes of fetal organs and tissues. The congenital malformations were noted in fetuses after injection of higher doses of Ascaris homogenate (exencephaly, craniomeningocele and intemal hydrocephalus). No malformations were noted in control groups and after injection of minimum dose of the homogenate. The symptoms that occurred after administration of the tegumental homogenate to pregnant mice included: decreased body weight gain (p<0.001) as compared to controls, vaginal hemorrhage, intrauterine resorption of litter and mortality. These signs suggest that the Ascaris homogenate causes matemal toxicity.
    Źródło:
    Annals of Parasitology; 2000, 46, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Disturbances of mouse pregnancy after injection of Ascaris homogenate during early organogenesis
    ZABURZENIA PRZEBIEGU CIĄŻY U MYSZY PO INIEKCJI HOMOGENATU Z ASCARIS W CZASIE WCZESNEJ ORGANOGENEZY
    Autorzy:
    Blaszkowska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148586.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    tegumental homogenate
    mouse
    mice
    Ascaris
    Ascaris suum
    organogenesis
    homogenate
    embryotoxicity
    pregnancy
    teratogenicity
    Opis:
    Administration of the Ascaris tegumental homogenate (0.6-1.2 g of Ascaris proteins/kg/day) at a early stage of organogenesis (5-9 days of pregnancy) had a harmful effect upon the developing mouse fetuses. It has been found that injection of the homogenate did not delay or prevent implantation, but causes a high rate of intrauterine deaths. The Ascaris homogenate significantly decreased the number of live fetuses per litter, increased the frequency of litter resorption, produced a delay in bone formation and induced pathological changes of fetal organs and tissues. The congenital malformations were noted in fetuses after injection of higher doses of Ascaris homogenate (exencephaly, craniomeningocele and intemal hydrocephalus). No malformations were noted in control groups and after injection of minimum dose of the homogenate. The symptoms that occurred after administration of the tegumental homogenate to pregnant mice included: decreased body weight gain (p<0.001) as compared to controls, vaginal hemorrhage, intrauterine resorption of litter and mortality. These signs suggest that the Ascaris homogenate causes matemal toxicity.
    Źródło:
    Wiadomości Parazytologiczne; 2000, 46, 3; 369-378
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of Ascaris trypsin inhibitor on proteolytic activity of lungs and kidneys of guinea pig with larval ascariosis and after ascarid homogenate administration
    Autorzy:
    Wojcik, A
    Kadlubowski, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/837331.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    trypsin inhibitor
    Ascaris
    ascarid homogenate
    ascariasis
    Ascaris suum
    guinea pig
    kidney
    larva
    lung
    proteolytic activity
    Opis:
    Trypsin inhibitor, isolated from Ascaris suum tegument reduces in vitro proteolytic activity (pH 7) of lungs and kidneys post-nuclear fractions of guinea pigs with larval ascariosis, pigs after subcutaneous ascarid homogenate administration as well as the activity in control groups. The comparative analysis of effect curves of trypsin inhibitor on the organ proteases revealed stronger reduction of the activity of post-nuclear lung proteases in ascariosis and of kidneys proteases after the injection of Ascaris homogenate than the inhibition of the fractions activity from respective control groups.
    Źródło:
    Annals of Parasitology; 2001, 47, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of Ascaris trypsin inhibitor on proteolytic activity of lungs and kidneys of guinea pig with larval ascariosis and after ascarid homogenate administration
    DZIAŁANIE INHIBITORA TRYPSYNY ASCARIS NA AKTYWNOŚĆ PROTEOLITYCZNĄ PŁUC I NEREK ŚWINEK MORSKICH Z ASKARYDOZĄ LARWALNĄ I PO PODANIU HOMOGENATU GLISTY
    Autorzy:
    Wójcik, A.
    Kadłubowski, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148472.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    trypsin inhibitor
    Ascaris
    ascarid homogenate
    ascariasis
    Ascaris suum
    guinea pig
    kidney
    larva
    lung
    proteolytic activity
    Opis:
    Trypsin inhibitor, isolated from Ascaris suum tegument reduces in vitro proteolytic activity (pH 7) of lungs and kidneys post-nuclear fractions of guinea pigs with larval ascariosis, pigs after subcutaneous ascarid homogenate administration as well as the activity in control groups. The comparative analysis of effect curves of trypsin inhibitor on the organ proteases revealed stronger reduction of the activity of post-nuclear lung proteases in ascariosis and of kidneys proteases after the injection of Ascaris homogenate than the inhibition of the fractions activity from respective control groups.
    Źródło:
    Wiadomości Parazytologiczne; 2001, 47, 4; 711-716
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Purification and characterisation of ferritin from the Baltic blue mussel Mytilus trossulus
    Autorzy:
    Potrykus, J.
    Kosakowska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/47730.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    Baltic Sea
    Baltic blue mussel
    Gdansk Gulf
    Mytilus trossulus
    acidification
    ammonium sulphate
    bovine serum albumin
    cadmium
    content
    heavy metal
    homogenate
    iron
    mussel
    protein
    purification
    thermal denaturation
    Opis:
    Baltic blue mussels Mytilus trossulus were collected from the Gulf of Gdańsk (southern Baltic Sea) in order to isolate ferritin from its soft tissues, as well as to purify and characterise this protein. Proteins were isolated from the inner organs of M. trossulus (hepatopancreas, gills and soft tissue residue) by thermal denaturation (70◦C) and acidification (pH 4.5) of the homogenates, followed by ammonium sulphate ((NH4)2SO4) fractionation. The ferritin was then separated by ultracentrifugation (100 000×g, 120 min.). The protein content in the purified homogenates was determined by the Lowry method using bovine serum albumin (BSA) and horse spleen ferritin (HSF) as standards. PAGE-SDS and Western blotting analysis permitted identification of ferritin in the purified preparations. Additionally, the purified homogenates and mussel soft tissue were analysed for their heavy metal contents (especially cadmium and iron) in a Video 11 E atomic absorption spectrophotometer, following wet digestion of the samples (HNO3/HClO4). The electrophoregrams showed that the inner organs of M. trossulus contained ferritin, which, like plant ferritin, is characterised by the presence of subunits in the electrophoregram in the 26.6–28.0 kDa range. The highest ferritin content was recorded in the hepatopancreas, followed by the gills and the soft tissue residue. With regard to the sampling stations, the highest content of ferritin was noted in the animals sampled off Sopot (station D3), and in those collected by a diver off Jastarnia (W1) and Gdynia (W4). Ferritin isolated from the inner organs of mussels collected from these stations also contained the largest quantities of heavy metals (Cd and Fe). Ferritin isolated from the inner organs of mussels collected by a diver from wrecks – sites where the concentrations of iron and other trace metals in the sea water are high – contained higher quantities of heavy metals (Cd and Fe) than the ferritin isolated from the inner organs of mussels collected with the drag. This confirms that ferritin is a protein able to store and transport not only iron, but also, though to a lesser extent, some other heavy metals, including cadmium.
    Źródło:
    Oceanologia; 2009, 51, 4
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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